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The map label for this gene is cofH [H]

Identifier: 118582017

GI number: 118582017

Start: 3999126

End: 4000199

Strand: Reverse

Name: cofH [H]

Synonym: Ppro_3618

Alternate gene names: 118582017

Gene position: 4000199-3999126 (Counterclockwise)

Preceding gene: 118582018

Following gene: 118582016

Centisome position: 99.81

GC content: 60.52

Gene sequence:

>1074_bases
ATGACACAAACCAGCACCCATGAAATACCAGCCACACACCACCGTCTGGAGCGGGACGAAGCGCTCCAACTCCTGAAACA
TGCCGATCTGCTGGCGTTGGGCAGGGAGGCGGATGGAATACGACGGCGCTATCATCCTGAAAACAGGGTTACCTTTGTCG
TTGACCGCAATGTTAACTACACCAACATCTGCGAATCCCGCTGCTCGTTCTGCGCATTCTACCGGGACGCCGCTGCTAAA
GACGCCTACGTTCTGACGCCGGACGAGATCTTCACCAAGATAACTGAACTGGTTGAGCTGGAAGGCACCCAACTGCTTAT
GCAGGGGGGGCTCAATCCTTCCCTCACCATCGATTACTTCGAGGAGCTGTTCCGCGAGATCGGCACGCGCTTTCCCTCGG
TGCAGAACCACTCGCTCTCACCTGCCGAAATCACCTGCATAGCAAGGGTTTCCGGACTGACCATCGATACCGCGCTCGAA
CGGCTGCATCGTGCCGGGCTTGATTCCATCCCGGGGGGGGGCGCCGAGATCCTGGTGGATGAGGTGCGCGGCGCTATATC
CCCCAACAAGATCGGCTGGCGCCAGTGGAGCAGGGTGATGCTCAAGGCGGCCAGGCTGGGCATGCCCACCACGGCCACCA
TGATGTTCGGCTGCGGAGAGCGGCTGGAAGACATCGTGGAGCATCTGTTCCGCGTGCGCGAACTGCAGGATGACGGCGGA
TCGTTCACCGCCTTTATCCCCTGGACCTACCAGCCCGGTAACACCGAGCTGGGGGGCGAAACCGCTACCGGAGTCGAATA
CCTGAAGGTCCTGGCTCTGTCGCGGATCGTGCTGGACAACATCCCCAACATCCAGGCCAGTTGGGTCACCCAGGGGGCCA
AGCTGGCGCAGGTTTCGCTCTCCTTTGGCGCAAACGATCTGGGGGGCACCATGCTGGAGGAAAACGTCGTGGCAGCCGCC
GGATGCCGCTTCCGCATGTCCCGCGACCAGATGGTGGCCCTGATCGCCGGGGCGGGATTCAAGGCCGCCCAACGCACGAC
AACGTACAAAATACTGAGGGAGTTTAGCGTTTGA

Upstream 100 bases:

>100_bases
AGTGCAGGATCGGCACGGACTCGGGGCTGCCCCGCGAATGACCTGCCGCGTCTTGAGGGAACGGATCTTGGGAACTACAA
ACAGAGAAATCGCAGAGTGA

Downstream 100 bases:

>100_bases
CATCACTGGAACAGCGGGCGGGGGCCTGCGAAATGATAACCGACGGAGGGAGGCTGGCCGAAGTGGCCGGCAGTATCAAT
TCCGAACGAGAAGTTGCCGT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTQTSTHEIPATHHRLERDEALQLLKHADLLALGREADGIRRRYHPENRVTFVVDRNVNYTNICESRCSFCAFYRDAAAK
DAYVLTPDEIFTKITELVELEGTQLLMQGGLNPSLTIDYFEELFREIGTRFPSVQNHSLSPAEITCIARVSGLTIDTALE
RLHRAGLDSIPGGGAEILVDEVRGAISPNKIGWRQWSRVMLKAARLGMPTTATMMFGCGERLEDIVEHLFRVRELQDDGG
SFTAFIPWTYQPGNTELGGETATGVEYLKVLALSRIVLDNIPNIQASWVTQGAKLAQVSLSFGANDLGGTMLEENVVAAA
GCRFRMSRDQMVALIAGAGFKAAQRTTTYKILREFSV

Sequences:

>Translated_357_residues
MTQTSTHEIPATHHRLERDEALQLLKHADLLALGREADGIRRRYHPENRVTFVVDRNVNYTNICESRCSFCAFYRDAAAK
DAYVLTPDEIFTKITELVELEGTQLLMQGGLNPSLTIDYFEELFREIGTRFPSVQNHSLSPAEITCIARVSGLTIDTALE
RLHRAGLDSIPGGGAEILVDEVRGAISPNKIGWRQWSRVMLKAARLGMPTTATMMFGCGERLEDIVEHLFRVRELQDDGG
SFTAFIPWTYQPGNTELGGETATGVEYLKVLALSRIVLDNIPNIQASWVTQGAKLAQVSLSFGANDLGGTMLEENVVAAA
GCRFRMSRDQMVALIAGAGFKAAQRTTTYKILREFSV
>Mature_356_residues
TQTSTHEIPATHHRLERDEALQLLKHADLLALGREADGIRRRYHPENRVTFVVDRNVNYTNICESRCSFCAFYRDAAAKD
AYVLTPDEIFTKITELVELEGTQLLMQGGLNPSLTIDYFEELFREIGTRFPSVQNHSLSPAEITCIARVSGLTIDTALER
LHRAGLDSIPGGGAEILVDEVRGAISPNKIGWRQWSRVMLKAARLGMPTTATMMFGCGERLEDIVEHLFRVRELQDDGGS
FTAFIPWTYQPGNTELGGETATGVEYLKVLALSRIVLDNIPNIQASWVTQGAKLAQVSLSFGANDLGGTMLEENVVAAAG
CRFRMSRDQMVALIAGAGFKAAQRTTTYKILREFSV

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39557; Mature: 39425

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTSTHEIPATHHRLERDEALQLLKHADLLALGREADGIRRRYHPENRVTFVVDRNVNY
CCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCCH
TNICESRCSFCAFYRDAAAKDAYVLTPDEIFTKITELVELEGTQLLMQGGLNPSLTIDYF
HHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEHHHH
EELFREIGTRFPSVQNHSLSPAEITCIARVSGLTIDTALERLHRAGLDSIPGGGAEILVD
HHHHHHHHHCCCCCCCCCCCCHHEEEEEHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
EVRGAISPNKIGWRQWSRVMLKAARLGMPTTATMMFGCGERLEDIVEHLFRVRELQDDGG
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCC
SFTAFIPWTYQPGNTELGGETATGVEYLKVLALSRIVLDNIPNIQASWVTQGAKLAQVSL
CEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEE
SFGANDLGGTMLEENVVAAAGCRFRMSRDQMVALIAGAGFKAAQRTTTYKILREFSV
ECCCCCCCCHHHHHCHHHHCCCEEECCHHHEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQTSTHEIPATHHRLERDEALQLLKHADLLALGREADGIRRRYHPENRVTFVVDRNVNY
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCCH
TNICESRCSFCAFYRDAAAKDAYVLTPDEIFTKITELVELEGTQLLMQGGLNPSLTIDYF
HHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEHHHH
EELFREIGTRFPSVQNHSLSPAEITCIARVSGLTIDTALERLHRAGLDSIPGGGAEILVD
HHHHHHHHHCCCCCCCCCCCCHHEEEEEHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
EVRGAISPNKIGWRQWSRVMLKAARLGMPTTATMMFGCGERLEDIVEHLFRVRELQDDGG
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCC
SFTAFIPWTYQPGNTELGGETATGVEYLKVLALSRIVLDNIPNIQASWVTQGAKLAQVSL
CEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEE
SFGANDLGGTMLEENVVAAAGCRFRMSRDQMVALIAGAGFKAAQRTTTYKILREFSV
ECCCCCCCCHHHHHCHHHHCCCEEECCHHHEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]