Click here to switch to the map view.

The map label for this gene is mlhB [H]

Identifier: 118581941

GI number: 118581941

Start: 3913728

End: 3914633

Strand: Direct

Name: mlhB [H]

Synonym: Ppro_3541

Alternate gene names: 118581941

Gene position: 3913728-3914633 (Clockwise)

Preceding gene: 118581940

Following gene: 118581942

Centisome position: 97.65

GC content: 67.22

Gene sequence:

>906_bases
GTGTTAAGTGAAAAGAGTAAACAGCTGCGGCTCTGGTTGACCAAGACCCTTAAAAACGCCCCCCCGCCCGCTTCGATCCA
GCAGGCGCGGGCGATCGTCGAATTCGGCGCCTCCCGGGCCGTCCTGCCTGCCGGCACCACGGTGGAACCGGTCAAGGCCG
GCGCGGTTCGGGCCGAATGGGTGCGCGCCGAGAATGTGGCCACGGACAGGGTGATCATGTACCTCCATGGCGGCGGCTTC
AGCAAGTGTTCCTGCAATACGGCCCGCATGACCGTGGCCCAGATATCGGCGGCCACGGGATGCAGCGTCCTGTCGGTTGA
ATACCGCCTGGCGCCGGAGAACCCCTTCCCCGCCGCCCTGGAAGATGCCCTGGCCGCCTACCGCTGGCTGCTGGAGCAGG
GCCATGACCCGCAGAAGCTGGTCCTGATGGGGGATTCCTCCGGCGGCGGTCTGGCGGTCTCCACCCTGGTCAGCCTGAAG
GCCGATGGCGACAGGCTCCCCTGTGCAGCGGTGCTGCTCTCCCCCTGGGTCGACCTGTCCTGCAGCGGGGAATCCATCAC
CAGCCGTGCCGAGATCGACCCCTGGATGACCGAGCAAGAGTGCCGCGCCTGCGCCGAGATGTACGCGCCGGGCGCCGACC
TGAAAAACCCCCTGATCTCTCCGATTCATGCCGACCTGCAGGGGTTGCCGCCGCTGTTGATTCATGTGGGGACCGACGAG
ATCCTGCTGGACGACTCCCAGCGCCTGGCCGGGAAGGCCAGGGAGGCCGGGGTGGAGGTGACCATGCACGTACAGGAGGG
GATGTGGCACGTGTGGCACTACTTCGCCCCCCACTTGCCGGAAGGGGTCGAGGCCATCGGCCAGGTGGGCGACTACGTCC
GCAGCCGCATGGTCGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
CTACCTGAAGTACGCGGTCTGCGTCGTCAGCCGGGAGCAGGGGCGCGTGCGCCGCTCCGTCTGACCTTTGGCACCATATC
ACGATAAAGGAGATCTCCCT

Downstream 100 bases:

>100_bases
GGCCGGGAAGCGCCGGGGCACACACCCCGCGTGGGTATAGGCTCTTTTGGCAGACAGGGAAAGGGGACTGTTGAGAGAGG
GGCTGACTGAACCTAGAATG

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLSEKSKQLRLWLTKTLKNAPPPASIQQARAIVEFGASRAVLPAGTTVEPVKAGAVRAEWVRAENVATDRVIMYLHGGGF
SKCSCNTARMTVAQISAATGCSVLSVEYRLAPENPFPAALEDALAAYRWLLEQGHDPQKLVLMGDSSGGGLAVSTLVSLK
ADGDRLPCAAVLLSPWVDLSCSGESITSRAEIDPWMTEQECRACAEMYAPGADLKNPLISPIHADLQGLPPLLIHVGTDE
ILLDDSQRLAGKAREAGVEVTMHVQEGMWHVWHYFAPHLPEGVEAIGQVGDYVRSRMVAGA

Sequences:

>Translated_301_residues
MLSEKSKQLRLWLTKTLKNAPPPASIQQARAIVEFGASRAVLPAGTTVEPVKAGAVRAEWVRAENVATDRVIMYLHGGGF
SKCSCNTARMTVAQISAATGCSVLSVEYRLAPENPFPAALEDALAAYRWLLEQGHDPQKLVLMGDSSGGGLAVSTLVSLK
ADGDRLPCAAVLLSPWVDLSCSGESITSRAEIDPWMTEQECRACAEMYAPGADLKNPLISPIHADLQGLPPLLIHVGTDE
ILLDDSQRLAGKAREAGVEVTMHVQEGMWHVWHYFAPHLPEGVEAIGQVGDYVRSRMVAGA
>Mature_301_residues
MLSEKSKQLRLWLTKTLKNAPPPASIQQARAIVEFGASRAVLPAGTTVEPVKAGAVRAEWVRAENVATDRVIMYLHGGGF
SKCSCNTARMTVAQISAATGCSVLSVEYRLAPENPFPAALEDALAAYRWLLEQGHDPQKLVLMGDSSGGGLAVSTLVSLK
ADGDRLPCAAVLLSPWVDLSCSGESITSRAEIDPWMTEQECRACAEMYAPGADLKNPLISPIHADLQGLPPLLIHVGTDE
ILLDDSQRLAGKAREAGVEVTMHVQEGMWHVWHYFAPHLPEGVEAIGQVGDYVRSRMVAGA

Specific function: Lactone hydrolase that is required for the degradation of monocyclic monoterpenes such as menthol and carvone. Catalyzes the ring opening of lactones formed during degradation of monocyclic monoterpenes. Can hydrolyze epsilon-caprolactone, the lactone der

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=155, Percent_Identity=28.3870967741935, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=238, Percent_Identity=26.4705882352941, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533410, Length=107, Percent_Identity=39.2523364485981, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI115533412, Length=107, Percent_Identity=39.2523364485981, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=154, Percent_Identity=32.4675324675325, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24656084, Length=101, Percent_Identity=35.6435643564356, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24656076, Length=101, Percent_Identity=35.6435643564356, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130169, Length=101, Percent_Identity=35.6435643564356, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.83 [H]

Molecular weight: Translated: 32317; Mature: 32317

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEKSKQLRLWLTKTLKNAPPPASIQQARAIVEFGASRAVLPAGTTVEPVKAGAVRAEW
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCHHCCCHHHHH
VRAENVATDRVIMYLHGGGFSKCSCNTARMTVAQISAATGCSVLSVEYRLAPENPFPAAL
HHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHH
EDALAAYRWLLEQGHDPQKLVLMGDSSGGGLAVSTLVSLKADGDRLPCAAVLLSPWVDLS
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEHHEECCCCCCCCCHHHHHHCCCCCEE
CSGESITSRAEIDPWMTEQECRACAEMYAPGADLKNPLISPIHADLQGLPPLLIHVGTDE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCC
ILLDDSQRLAGKAREAGVEVTMHVQEGMWHVWHYFAPHLPEGVEAIGQVGDYVRSRMVAG
EEECCCHHHHHHHHHCCCEEEEEHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure
MLSEKSKQLRLWLTKTLKNAPPPASIQQARAIVEFGASRAVLPAGTTVEPVKAGAVRAEW
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCHHCCCHHHHH
VRAENVATDRVIMYLHGGGFSKCSCNTARMTVAQISAATGCSVLSVEYRLAPENPFPAAL
HHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHH
EDALAAYRWLLEQGHDPQKLVLMGDSSGGGLAVSTLVSLKADGDRLPCAAVLLSPWVDLS
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEHHEECCCCCCCCCHHHHHHCCCCCEE
CSGESITSRAEIDPWMTEQECRACAEMYAPGADLKNPLISPIHADLQGLPPLLIHVGTDE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCC
ILLDDSQRLAGKAREAGVEVTMHVQEGMWHVWHYFAPHLPEGVEAIGQVGDYVRSRMVAG
EEECCCHHHHHHHHHCCCEEEEEHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA