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The map label for this gene is rlmI [H]

Identifier: 118581940

GI number: 118581940

Start: 3912462

End: 3913691

Strand: Direct

Name: rlmI [H]

Synonym: Ppro_3540

Alternate gene names: 118581940

Gene position: 3912462-3913691 (Clockwise)

Preceding gene: 118581939

Following gene: 118581941

Centisome position: 97.62

GC content: 67.56

Gene sequence:

>1230_bases
ATGGGCATGGATACGAGGATTGTGGGGCCGGAAACGGTGCGCATGCTGGAACTGGGACACCCCTGGATCATAGCCGACAG
CCACACCAGGCGCTGGCCGTCTGGCCAGGCTGGCCAGACGGTGGCGCTGACCGATGCCACGGGACGTTTTCTGGCAACCG
CCCTGCTGGACCCGGACGACCGCATCGTGGCACGGGTGCTGTCGCATGAGCGGATACGGCTGGATGCCGCCTGGATCAGG
CGTCGCCTGGAGGCTAGCCTTACCCTGCGCCGCGACCACGCCGACCTGGGGGAGAGCGACGCCTTCCGGCTGGTGAACGC
CGAGGGGGACGGCCTGCCCGGCCTGACGGTGGATTGCTACGGCCAGTTCCTGATGGTGCAGCTCTACTGCGCCGGCTGGC
GCCCCCATCTGAAGACCCTGTCCGGAGAGCTGATGGAGCTGCTGCAGCCCCGCGGCATCTACGAGAAGAGCAGGCCGCAG
AAGACAAGGGAGCTGGAGGCGGCAAGAGACGACAAGCGCTACGGCCGGCTGCTCTCGGGCGAGTCGGCGCCCCAGCGGCT
GCAGGTGCGCGAGAACGGCCTCTCCTTCCTGGTCAGCCTGGAGCAGGGCCTGAATACCGGCCTGTTCCTGGACCAGCGCC
GCAACCGGCGCGACCTGATGAACCGGGTGGAGGGGAAGCGGGTGCTGAACCTGTTCGCCTACACCGGCGCCTTTTCCGTG
GCCGCCGCGTCGGCCGGCGCCCGGGTGACCAGCGTGGACGTGTCGGCCGGCTACCTGGAATGGGCCAGGGACAACTTCGG
CATCAACCGGCTCAACCCAAAGCGCCACGAGTTCATCACCGGCGACTGCCTGGCGGTGCTGTGCCAGCTTGCCCAAAAAG
GGCAGGGCTTTGACGTGATCCTGATGGATCCCCCCTCCTTCTCCACCACCTCCAAGAGTCGCTTCACCACCCGCGGCGGC
ACGGCGGAGCTGGTGGCGGCGGCGTTGCCGCTTCTGGCCGATGGCGGGCTACTGATCGCCTCCTCCAACCACCAGAAGCT
GGAGGTGAACGACTATCTCAAGGAGTTGCGCCGCGGCGCGCTCATGGCCGGTTGCGAGCTGCGGGTAATTTCCCTGTCCG
GCCAGCCGGAAGATTTCCCCTATGGGGTCTGCTTTCCCGAGGGGCGCTACCTGAAGTACGCGGTCTGCGTCGTCAGCCGG
GAGCAGGGGCGCGTGCGCCGCTCCGTCTGA

Upstream 100 bases:

>100_bases
TGGAGATAGACCTGGAGGATTTCGGCCAGGCTGAGCAGCGGCTGGAGGCGTTCCTGGCCGAGCTCAAGAAACCTGCGCCA
GGGGATGGCGAGGGGTGACA

Downstream 100 bases:

>100_bases
CCTTTGGCACCATATCACGATAAAGGAGATCTCCCTGTGTTAAGTGAAAAGAGTAAACAGCTGCGGCTCTGGTTGACCAA
GACCCTTAAAAACGCCCCCC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MGMDTRIVGPETVRMLELGHPWIIADSHTRRWPSGQAGQTVALTDATGRFLATALLDPDDRIVARVLSHERIRLDAAWIR
RRLEASLTLRRDHADLGESDAFRLVNAEGDGLPGLTVDCYGQFLMVQLYCAGWRPHLKTLSGELMELLQPRGIYEKSRPQ
KTRELEAARDDKRYGRLLSGESAPQRLQVRENGLSFLVSLEQGLNTGLFLDQRRNRRDLMNRVEGKRVLNLFAYTGAFSV
AAASAGARVTSVDVSAGYLEWARDNFGINRLNPKRHEFITGDCLAVLCQLAQKGQGFDVILMDPPSFSTTSKSRFTTRGG
TAELVAAALPLLADGGLLIASSNHQKLEVNDYLKELRRGALMAGCELRVISLSGQPEDFPYGVCFPEGRYLKYAVCVVSR
EQGRVRRSV

Sequences:

>Translated_409_residues
MGMDTRIVGPETVRMLELGHPWIIADSHTRRWPSGQAGQTVALTDATGRFLATALLDPDDRIVARVLSHERIRLDAAWIR
RRLEASLTLRRDHADLGESDAFRLVNAEGDGLPGLTVDCYGQFLMVQLYCAGWRPHLKTLSGELMELLQPRGIYEKSRPQ
KTRELEAARDDKRYGRLLSGESAPQRLQVRENGLSFLVSLEQGLNTGLFLDQRRNRRDLMNRVEGKRVLNLFAYTGAFSV
AAASAGARVTSVDVSAGYLEWARDNFGINRLNPKRHEFITGDCLAVLCQLAQKGQGFDVILMDPPSFSTTSKSRFTTRGG
TAELVAAALPLLADGGLLIASSNHQKLEVNDYLKELRRGALMAGCELRVISLSGQPEDFPYGVCFPEGRYLKYAVCVVSR
EQGRVRRSV
>Mature_408_residues
GMDTRIVGPETVRMLELGHPWIIADSHTRRWPSGQAGQTVALTDATGRFLATALLDPDDRIVARVLSHERIRLDAAWIRR
RLEASLTLRRDHADLGESDAFRLVNAEGDGLPGLTVDCYGQFLMVQLYCAGWRPHLKTLSGELMELLQPRGIYEKSRPQK
TRELEAARDDKRYGRLLSGESAPQRLQVRENGLSFLVSLEQGLNTGLFLDQRRNRRDLMNRVEGKRVLNLFAYTGAFSVA
AASAGARVTSVDVSAGYLEWARDNFGINRLNPKRHEFITGDCLAVLCQLAQKGQGFDVILMDPPSFSTTSKSRFTTRGGT
AELVAAALPLLADGGLLIASSNHQKLEVNDYLKELRRGALMAGCELRVISLSGQPEDFPYGVCFPEGRYLKYAVCVVSRE
QGRVRRSV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=385, Percent_Identity=33.5064935064935, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1787181, Length=260, Percent_Identity=33.4615384615385, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45461; Mature: 45329

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMDTRIVGPETVRMLELGHPWIIADSHTRRWPSGQAGQTVALTDATGRFLATALLDPDD
CCCCCEEECHHHEEEEECCCCEEEECCCCCCCCCCCCCCEEEEECCCCCEEEHHHCCCCH
RIVARVLSHERIRLDAAWIRRRLEASLTLRRDHADLGESDAFRLVNAEGDGLPGLTVDCY
HHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHH
GQFLMVQLYCAGWRPHLKTLSGELMELLQPRGIYEKSRPQKTRELEAARDDKRYGRLLSG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ESAPQRLQVRENGLSFLVSLEQGLNTGLFLDQRRNRRDLMNRVEGKRVLNLFAYTGAFSV
CCCCHHHHHHHCCCEEEEEHHHCCCCCEEECCCCCHHHHHHHHCHHHHHHHHHHHCHHHH
AAASAGARVTSVDVSAGYLEWARDNFGINRLNPKRHEFITGDCLAVLCQLAQKGQGFDVI
HHHCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCHHHEECHHHHHHHHHHHHCCCCCEEE
LMDPPSFSTTSKSRFTTRGGTAELVAAALPLLADGGLLIASSNHQKLEVNDYLKELRRGA
EECCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHHHHHCC
LMAGCELRVISLSGQPEDFPYGVCFPEGRYLKYAVCVVSREQGRVRRSV
EEECCEEEEEEECCCCCCCCEEEECCCCCEEEEEEEEEECCCCCHHCCC
>Mature Secondary Structure 
GMDTRIVGPETVRMLELGHPWIIADSHTRRWPSGQAGQTVALTDATGRFLATALLDPDD
CCCCEEECHHHEEEEECCCCEEEECCCCCCCCCCCCCCEEEEECCCCCEEEHHHCCCCH
RIVARVLSHERIRLDAAWIRRRLEASLTLRRDHADLGESDAFRLVNAEGDGLPGLTVDCY
HHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHH
GQFLMVQLYCAGWRPHLKTLSGELMELLQPRGIYEKSRPQKTRELEAARDDKRYGRLLSG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ESAPQRLQVRENGLSFLVSLEQGLNTGLFLDQRRNRRDLMNRVEGKRVLNLFAYTGAFSV
CCCCHHHHHHHCCCEEEEEHHHCCCCCEEECCCCCHHHHHHHHCHHHHHHHHHHHCHHHH
AAASAGARVTSVDVSAGYLEWARDNFGINRLNPKRHEFITGDCLAVLCQLAQKGQGFDVI
HHHCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCHHHEECHHHHHHHHHHHHCCCCCEEE
LMDPPSFSTTSKSRFTTRGGTAELVAAALPLLADGGLLIASSNHQKLEVNDYLKELRRGA
EECCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHHHHHCC
LMAGCELRVISLSGQPEDFPYGVCFPEGRYLKYAVCVVSREQGRVRRSV
EEECCEEEEEEECCCCCCCCEEEECCCCCEEEEEEEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA