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The map label for this gene is selU [H]

Identifier: 118581939

GI number: 118581939

Start: 3911410

End: 3912459

Strand: Direct

Name: selU [H]

Synonym: Ppro_3539

Alternate gene names: 118581939

Gene position: 3911410-3912459 (Clockwise)

Preceding gene: 118581934

Following gene: 118581940

Centisome position: 97.59

GC content: 64.48

Gene sequence:

>1050_bases
ATGCCTGAAACGTTATCATTCACACCGGAGCTGCTGGACAGCCACTGTATCGTCGATGTCAGGACGCCACTGGAGTTCAT
TGAGGACCACCTGCCGGGGGCCGTCAATGTTCCGATCCTGACCGATCAGGAGCGGGTGGAGATCGGCACCATCCACAACC
GGATCGGGCCGCGGGAGGCCCGCATGCGCGGACTGGAGCTGACCTGCGGCCGCTTCGCCGACATGGTGCGTCAGGTGTTT
GGCCTTGCCCAGGGGCGGCCGACACTGGTCTACTGCTGGCGTGGAGGGCTGCGCAGCCGCTCCATGGCAGTCCTGCTGGA
GGCAACTGGCCAGCGGGTGTTCCAGCTTGCCGGCGGCTACAAGTCCTTCCGCAATCATGTCAACGATTACTTCCAGTGTT
TTGAGCCGCAGGCTCCCCTGATCGTCCTGCACGGCATGACCGGAACCGGCAAGACGACCTTTCTCAACCGCCTCGACCGC
GGGAAGTGGACTCCCATCGACCTGGAGGGGTTGGCCTGCCACCGCGGCTCGGCCTTCGGCTCCCTGGGACTCGAACAGGA
CCTGAGCCAGAAGCGCTTCGACACGCTGCTCTGGGAAGCCTTCCGTAAGGCGCCAGTCGACCGCCCCATCGTCCTGGAGG
GGGAGAGCCAGCGCATCGGCAGGATCATGCTCCCCGGACGGCTGTATGAGGCCATGTCCGACGGCTACCGCATCTGGTGC
AGCGCCAGCCTGGAGACCCGCGTCAAACGCCTGGCTGAGGAGTATGCCCGCGAGGAGTACCGTGACGAGATGGCCGCGTC
GCTCACCCGCATCAGGAAGAAGCTGGGTGGTCCCCACTACGCCGAACTGTCCGGGATGCTTGAGCGCTGGGATGTGGAGG
GGCTGGGACGTGGGTTGATCGAGCAGTACTACGACAAGCTCTACTACAAGCACCGCCCCTGGGAGCCGGATCTGGAGATA
GACCTGGAGGATTTCGGCCAGGCTGAGCAGCGGCTGGAGGCGTTCCTGGCCGAGCTCAAGAAACCTGCGCCAGGGGATGG
CGAGGGGTGA

Upstream 100 bases:

>100_bases
CCGAAGCGAATCAAGGAGTAAGCCATGGACTTTTCAGTTACTTCCCTGTTGTCAGCAGGGCGTGAGTAGCCTATACTCGC
CGGCCGGAGAGTACAAGCAG

Downstream 100 bases:

>100_bases
CAATGGGCATGGATACGAGGATTGTGGGGCCGGAAACGGTGCGCATGCTGGAACTGGGACACCCCTGGATCATAGCCGAC
AGCCACACCAGGCGCTGGCC

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MPETLSFTPELLDSHCIVDVRTPLEFIEDHLPGAVNVPILTDQERVEIGTIHNRIGPREARMRGLELTCGRFADMVRQVF
GLAQGRPTLVYCWRGGLRSRSMAVLLEATGQRVFQLAGGYKSFRNHVNDYFQCFEPQAPLIVLHGMTGTGKTTFLNRLDR
GKWTPIDLEGLACHRGSAFGSLGLEQDLSQKRFDTLLWEAFRKAPVDRPIVLEGESQRIGRIMLPGRLYEAMSDGYRIWC
SASLETRVKRLAEEYAREEYRDEMAASLTRIRKKLGGPHYAELSGMLERWDVEGLGRGLIEQYYDKLYYKHRPWEPDLEI
DLEDFGQAEQRLEAFLAELKKPAPGDGEG

Sequences:

>Translated_349_residues
MPETLSFTPELLDSHCIVDVRTPLEFIEDHLPGAVNVPILTDQERVEIGTIHNRIGPREARMRGLELTCGRFADMVRQVF
GLAQGRPTLVYCWRGGLRSRSMAVLLEATGQRVFQLAGGYKSFRNHVNDYFQCFEPQAPLIVLHGMTGTGKTTFLNRLDR
GKWTPIDLEGLACHRGSAFGSLGLEQDLSQKRFDTLLWEAFRKAPVDRPIVLEGESQRIGRIMLPGRLYEAMSDGYRIWC
SASLETRVKRLAEEYAREEYRDEMAASLTRIRKKLGGPHYAELSGMLERWDVEGLGRGLIEQYYDKLYYKHRPWEPDLEI
DLEDFGQAEQRLEAFLAELKKPAPGDGEG
>Mature_348_residues
PETLSFTPELLDSHCIVDVRTPLEFIEDHLPGAVNVPILTDQERVEIGTIHNRIGPREARMRGLELTCGRFADMVRQVFG
LAQGRPTLVYCWRGGLRSRSMAVLLEATGQRVFQLAGGYKSFRNHVNDYFQCFEPQAPLIVLHGMTGTGKTTFLNRLDRG
KWTPIDLEGLACHRGSAFGSLGLEQDLSQKRFDTLLWEAFRKAPVDRPIVLEGESQRIGRIMLPGRLYEAMSDGYRIWCS
ASLETRVKRLAEEYAREEYRDEMAASLTRIRKKLGGPHYAELSGMLERWDVEGLGRGLIEQYYDKLYYKHRPWEPDLEID
LEDFGQAEQRLEAFLAELKKPAPGDGEG

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=325, Percent_Identity=31.6923076923077, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 39779; Mature: 39648

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETLSFTPELLDSHCIVDVRTPLEFIEDHLPGAVNVPILTDQERVEIGTIHNRIGPREA
CCCCCCCCHHHHCCCCEEEECCHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHCCCCHHH
RMRGLELTCGRFADMVRQVFGLAQGRPTLVYCWRGGLRSRSMAVLLEATGQRVFQLAGGY
HHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCH
KSFRNHVNDYFQCFEPQAPLIVLHGMTGTGKTTFLNRLDRGKWTPIDLEGLACHRGSAFG
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCC
SLGLEQDLSQKRFDTLLWEAFRKAPVDRPIVLEGESQRIGRIMLPGRLYEAMSDGYRIWC
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHCCCEEEEE
SASLETRVKRLAEEYAREEYRDEMAASLTRIRKKLGGPHYAELSGMLERWDVEGLGRGLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHH
EQYYDKLYYKHRPWEPDLEIDLEDFGQAEQRLEAFLAELKKPAPGDGEG
HHHHHHHHHCCCCCCCCCEECHHHHCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PETLSFTPELLDSHCIVDVRTPLEFIEDHLPGAVNVPILTDQERVEIGTIHNRIGPREA
CCCCCCCHHHHCCCCEEEECCHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHCCCCHHH
RMRGLELTCGRFADMVRQVFGLAQGRPTLVYCWRGGLRSRSMAVLLEATGQRVFQLAGGY
HHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCH
KSFRNHVNDYFQCFEPQAPLIVLHGMTGTGKTTFLNRLDRGKWTPIDLEGLACHRGSAFG
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCC
SLGLEQDLSQKRFDTLLWEAFRKAPVDRPIVLEGESQRIGRIMLPGRLYEAMSDGYRIWC
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHCCCEEEEE
SASLETRVKRLAEEYAREEYRDEMAASLTRIRKKLGGPHYAELSGMLERWDVEGLGRGLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHH
EQYYDKLYYKHRPWEPDLEIDLEDFGQAEQRLEAFLAELKKPAPGDGEG
HHHHHHHHHCCCCCCCCCEECHHHHCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA