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The map label for this gene is mnhA1 [H]

Identifier: 118581932

GI number: 118581932

Start: 3902875

End: 3904317

Strand: Reverse

Name: mnhA1 [H]

Synonym: Ppro_3532

Alternate gene names: 118581932

Gene position: 3904317-3902875 (Counterclockwise)

Preceding gene: 118581933

Following gene: 118581931

Centisome position: 97.41

GC content: 60.43

Gene sequence:

>1443_bases
ATGAAAACCAACATCATTGTGTGGATGGCTGTTTTCGTTCCGATCGCGGGAGCTTTTCTACTTCCCCTCATGGGCCAGCT
GTCCAAGGGGCTCAGAAACCTGGGCGCCCTTGCCCTGGTTACCTCTTCCCTGATCTGCTCGTCATCCCTGGTTCCCTTGG
TGCTGGCCGGCGGGACGGAGAGCATCTCCATTCCCTTTCTCGGGGGGAACAACCTCTTTCTGGCGGACAGCCTGGCGGTC
TTCATGGCCATTGTCTCCATGCTGGTGGGTGCCGTCATCGTGCTCTACTCCATGGGCTACATCAGCCATTACGATAACCA
GAACGAATACTATTTCATGGTGGTGCTGTTCCTCGGCTCCATGATGGGGATCGTCTACTCGGCCAACCTGATCCTGATCT
ACCTGTTCTGGGAGATCACCGCCATCGCCTGCTGGCGCCTGATCGGATTCTTCCGCGAGCCGCTCTGCGTGGTCAGGGCC
GACAAGGCCTTCCTGATGACCGGCTTCGGCGCCTTTGTCATGCTGATCGGCTTCATCATGATCCACGGCCAGTACGGCTC
CTTCGACCTGCAGGTGATCAAGAGCGCCTCGGCAGCCAACCCGGTGGCCAATCTGGCCGTGCTGCTGATCCTCTTCGGCA
TCCTCTCCAAATCGGCTACCTTCCCCCTGCACACCTGGCTTCCCGACGCGGGCGTGGCCCCCACGCCGGTGACCGCCCTG
CTGCACGCCGCGGTGCTGGTCAAGATCGGCGTGTACGTCTATGCCAGGCTGTTTGTTGCCTCGTTCACGGTGGACCCGGC
CTGGAACAGCGTCGTGCCGATGATCGCCGGCATCAGCGCCCTGGTCTCGGGCGGGGCTGCCCTGATCGAGACCGACATGA
AACGCATCATCGCCTACTCCACGGTCAGCCAGTTGGGGTTCATCCTGCTGGGGCTTTCCATCGGCAATCCCCTGGGATTC
GCCGGAGGACTATTGTACATCCTGATGCACAGCATCGCCAAGGGGGGCATCTTCCTCTGTGCCGGTGTGGTGGAGCAGAA
GCTGCACGTAAAAGACATCACCAAGCTGGGGGGGCTGATCAAGACCATGCCGGTGACCGCGGTTTCCTTCCTGCTCTGCT
CCTTTTCAGTCATGGGCATTCCCCCCTTCGGCGGATTCTTCAGCAAGTACATGGTCATCGCCGGAGCGATCAGGAGCGGC
GACTACCTGCTGACGACCATCTTCATCCTGGGGGCCTTCCTGACGGTGATCTATCTGTTCAGGATCTTTGCCATGATCTT
CCTGGGTGAGCCCAAGACCACTCCCGTTGCCGAGGGTGCCCCGGTCATGCTGGTGAGCGTCGCCCTGCTGGCGCTGCTCT
CCCTGGCAGGAGGGCTGCTGATCAGTTATCCCTCGCTCTATGTCCAGACCGCCGTACAGCAGATCATGGGGGTGACGCCA
TGA

Upstream 100 bases:

>100_bases
GTTGGAATTGGAATCACACGTCATTTAGCGGGTCATCTTTTCACCATCAGGGAAACCACGGAACCAGATCGTTCTCTTGC
GGGGGAGGGTGGAGTACGTC

Downstream 100 bases:

>100_bases
CGGAAAGCATGATGATGTGCGCCATACTGCTTCCGGCCCTGTGTGGATCAGTGGCACTGGTTCTCCCCAAGGGGGGGCAG
GGCGCACTGGCCCTGCTGGC

Product: NADH dehydrogenase (quinone)

Products: NAD+; ubiquinol [C]

Alternate protein names: Mnh complex subunit A1 [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKTNIIVWMAVFVPIAGAFLLPLMGQLSKGLRNLGALALVTSSLICSSSLVPLVLAGGTESISIPFLGGNNLFLADSLAV
FMAIVSMLVGAVIVLYSMGYISHYDNQNEYYFMVVLFLGSMMGIVYSANLILIYLFWEITAIACWRLIGFFREPLCVVRA
DKAFLMTGFGAFVMLIGFIMIHGQYGSFDLQVIKSASAANPVANLAVLLILFGILSKSATFPLHTWLPDAGVAPTPVTAL
LHAAVLVKIGVYVYARLFVASFTVDPAWNSVVPMIAGISALVSGGAALIETDMKRIIAYSTVSQLGFILLGLSIGNPLGF
AGGLLYILMHSIAKGGIFLCAGVVEQKLHVKDITKLGGLIKTMPVTAVSFLLCSFSVMGIPPFGGFFSKYMVIAGAIRSG
DYLLTTIFILGAFLTVIYLFRIFAMIFLGEPKTTPVAEGAPVMLVSVALLALLSLAGGLLISYPSLYVQTAVQQIMGVTP

Sequences:

>Translated_480_residues
MKTNIIVWMAVFVPIAGAFLLPLMGQLSKGLRNLGALALVTSSLICSSSLVPLVLAGGTESISIPFLGGNNLFLADSLAV
FMAIVSMLVGAVIVLYSMGYISHYDNQNEYYFMVVLFLGSMMGIVYSANLILIYLFWEITAIACWRLIGFFREPLCVVRA
DKAFLMTGFGAFVMLIGFIMIHGQYGSFDLQVIKSASAANPVANLAVLLILFGILSKSATFPLHTWLPDAGVAPTPVTAL
LHAAVLVKIGVYVYARLFVASFTVDPAWNSVVPMIAGISALVSGGAALIETDMKRIIAYSTVSQLGFILLGLSIGNPLGF
AGGLLYILMHSIAKGGIFLCAGVVEQKLHVKDITKLGGLIKTMPVTAVSFLLCSFSVMGIPPFGGFFSKYMVIAGAIRSG
DYLLTTIFILGAFLTVIYLFRIFAMIFLGEPKTTPVAEGAPVMLVSVALLALLSLAGGLLISYPSLYVQTAVQQIMGVTP
>Mature_480_residues
MKTNIIVWMAVFVPIAGAFLLPLMGQLSKGLRNLGALALVTSSLICSSSLVPLVLAGGTESISIPFLGGNNLFLADSLAV
FMAIVSMLVGAVIVLYSMGYISHYDNQNEYYFMVVLFLGSMMGIVYSANLILIYLFWEITAIACWRLIGFFREPLCVVRA
DKAFLMTGFGAFVMLIGFIMIHGQYGSFDLQVIKSASAANPVANLAVLLILFGILSKSATFPLHTWLPDAGVAPTPVTAL
LHAAVLVKIGVYVYARLFVASFTVDPAWNSVVPMIAGISALVSGGAALIETDMKRIIAYSTVSQLGFILLGLSIGNPLGF
AGGLLYILMHSIAKGGIFLCAGVVEQKLHVKDITKLGGLIKTMPVTAVSFLLCSFSVMGIPPFGGFFSKYMVIAGAIRSG
DYLLTTIFILGAFLTVIYLFRIFAMIFLGEPKTTPVAEGAPVMLVSVALLALLSLAGGLLISYPSLYVQTAVQQIMGVTP

Specific function: Mnh complex is a Na(+)/H(+) antiporter involved in Na(+) excretion [H]

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=404, Percent_Identity=30.6930693069307, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI251831116, Length=265, Percent_Identity=27.5471698113208, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788614, Length=424, Percent_Identity=32.5471698113208, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1788829, Length=385, Percent_Identity=31.9480519480519, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788827, Length=427, Percent_Identity=30.4449648711944, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788831, Length=375, Percent_Identity=31.2, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788613, Length=462, Percent_Identity=25.974025974026, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI2367154, Length=396, Percent_Identity=27.7777777777778, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI145693160, Length=347, Percent_Identity=25.0720461095101, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 51113; Mature: 51113

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNIIVWMAVFVPIAGAFLLPLMGQLSKGLRNLGALALVTSSLICSSSLVPLVLAGGTE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
SISIPFLGGNNLFLADSLAVFMAIVSMLVGAVIVLYSMGYISHYDNQNEYYFMVVLFLGS
EEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
MMGIVYSANLILIYLFWEITAIACWRLIGFFREPLCVVRADKAFLMTGFGAFVMLIGFIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHH
IHGQYGSFDLQVIKSASAANPVANLAVLLILFGILSKSATFPLHTWLPDAGVAPTPVTAL
HCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHH
LHAAVLVKIGVYVYARLFVASFTVDPAWNSVVPMIAGISALVSGGAALIETDMKRIIAYS
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TVSQLGFILLGLSIGNPLGFAGGLLYILMHSIAKGGIFLCAGVVEQKLHVKDITKLGGLI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHH
KTMPVTAVSFLLCSFSVMGIPPFGGFFSKYMVIAGAIRSGDYLLTTIFILGAFLTVIYLF
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RIFAMIFLGEPKTTPVAEGAPVMLVSVALLALLSLAGGLLISYPSLYVQTAVQQIMGVTP
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTNIIVWMAVFVPIAGAFLLPLMGQLSKGLRNLGALALVTSSLICSSSLVPLVLAGGTE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
SISIPFLGGNNLFLADSLAVFMAIVSMLVGAVIVLYSMGYISHYDNQNEYYFMVVLFLGS
EEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
MMGIVYSANLILIYLFWEITAIACWRLIGFFREPLCVVRADKAFLMTGFGAFVMLIGFIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHH
IHGQYGSFDLQVIKSASAANPVANLAVLLILFGILSKSATFPLHTWLPDAGVAPTPVTAL
HCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHH
LHAAVLVKIGVYVYARLFVASFTVDPAWNSVVPMIAGISALVSGGAALIETDMKRIIAYS
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TVSQLGFILLGLSIGNPLGFAGGLLYILMHSIAKGGIFLCAGVVEQKLHVKDITKLGGLI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHH
KTMPVTAVSFLLCSFSVMGIPPFGGFFSKYMVIAGAIRSGDYLLTTIFILGAFLTVIYLF
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RIFAMIFLGEPKTTPVAEGAPVMLVSVALLALLSLAGGLLISYPSLYVQTAVQQIMGVTP
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA