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The map label for this gene is mrpA [H]

Identifier: 118581931

GI number: 118581931

Start: 3901046

End: 3902878

Strand: Reverse

Name: mrpA [H]

Synonym: Ppro_3531

Alternate gene names: 118581931

Gene position: 3902878-3901046 (Counterclockwise)

Preceding gene: 118581932

Following gene: 118581930

Centisome position: 97.38

GC content: 62.3

Gene sequence:

>1833_bases
ATGACGGAAAGCATGATGATGTGCGCCATACTGCTTCCGGCCCTGTGTGGATCAGTGGCACTGGTTCTCCCCAAGGGGGG
GCAGGGCGCACTGGCCCTGCTGGCAACGCTCGCCAACCTCCTGATCTGTCTGTGGATGTTCGGCCATGAGGTGCTGCTCT
CCCTCCCCTGGGCCGGTTTCGGCATGCTGTTCGAGCTGCGCCTGTACCATTTCAGCGCCTTCATCCTGGCCGCCGCCGCC
GGTTTTGCCCTTCTGGTCTCCCTCTACTCCCTGCACTTCATGGCCAGGCGCAGCCACACCAACCAGTTCTATGCCTACCT
GCTGATTTCCCTGGGGATGGTCAACGGCGCGGTGCTGGCCGACAACCTGATCCTGATGCTCTTCTTCTGGGAAGGACTGC
TGTGCACCATTTTCGGATTCATCGTCATCGGCAGCGGGGAATCATTCCGTTCGGCCATCAAGGCCTTTGTCCTGATCGGC
GTATCCGACATCCTGATGATGGTCGGTATCGGCATGACCGGCTTTTTGGCAGGCACCATGACCATCAGCCGCATCCACCT
CAGCCCCGATTCGGCACTGGCCGGAGCCGCTTTCCTGCTGCTGGTGATCGGCGCAACATCCAAGGCCGGCAGCATGCCCT
TCCACAGCTGGATTCCGGACGCGGCGGTGGATGCACCGCTCCCCTTCATGGCCCTGATGCCGGCGGCCCTGGAGAAGCTT
TTGGGGATCTACTTCCTCTCCCGCATCACCCTGGACCTGTTCGCCCTCTCCCCCGCTTCCTGGCTCAGCCCGCTGCTCAT
GACCCTGGGGGTGGTCACCATCCTGCTGGCGGTCATGATGGCCCTGATCCAGAAGGACTACAAGCGGCTGCTCTCCTACC
ACGCCATCAGCCAGGTTGGCTACATGATCCTGGGCATCGGCACTGCGGTCCCGGTGGGGATTGTTGGCGGCATCTTCCAC
ATGATTAACCACGCCATGTACAAGAGCTGCCTGTTCCTGACCAGCGGCTCGGTGGAGGCCCAGACCGGCACCACCGACCT
GAAGAAGCTGGGTGGCATGGGCATGCGCATGCCGATCACCTTCATCTGCTTCATCATCGCCGCCGCCGCCATATCCGGCG
TGCCCCCCTTCAACGGCTTCTTCTCCAAGGAACTGATCTACGACGGCGCCCTGGAGCGGCACTGGATTTTCTACGCGGGC
GCCCTGGCCGGATCGTTCCTGACCGCGGCATCATTCCTCAAGCTGGGACATGCGGTCTTTCTCGGCAAGGCCGAGGATGA
GGCTTCCTTCTCCCTGGTCAGGGAGGCGCCGCTCTCCATGCTGATCCCCATGGTCACTATCGCCGGTGGATGCGTCCTCT
TCGGGGTGGCCAACCGGCTTCCGCTGGACACCCTGATCGTCCCAATCATCGGCCCCCAGCTGGCGGCCGGGCACCATTTC
GGCGGATTCCCCCAGAACCGCTTCCTGGTATTCATGACCATGCTGGTGCTCTTCGCTGCCTGGATCAACCACCTCTACGG
CGTCAGGAAGAGTGGCACGGGAGTCGGGGCGTCCGATCACATCCACTACTCGCCCCTGCTGCATCCGGTCTACGAGCGTG
CCGAACGGCGCCTGTTCGATCCCTACGACCTGGGCATGAAAGTTGTCCAGGGCTTCTCCCGGCTGGCGTGGTGGATCGAT
CGCGCCAACGACTGGCTGTTCAATGTCATGGCGGTGCGGACGGCGCAGTTCTGCTCCCGTTCCCTGTCCGCGCTGCATAA
CGGCAGTTACGCCACCTACATGGCCTGGGTGGCCATTGGCCTGATCCTGATGGGGCTGTACCTGAATGTGTGA

Upstream 100 bases:

>100_bases
TCGCCCTGCTGGCGCTGCTCTCCCTGGCAGGAGGGCTGCTGATCAGTTATCCCTCGCTCTATGTCCAGACCGCCGTACAG
CAGATCATGGGGGTGACGCC

Downstream 100 bases:

>100_bases
CACAAGGAGGATAGTGGGATGAGCTCGCTTCTGCTTGTTCTTCCGTTGTTAGGCGTAATCGCATCGAACCTCCCCCTGGG
GCGTTCCACGAGGCGGGCCT

Product: NADH dehydrogenase (quinone)

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTESMMMCAILLPALCGSVALVLPKGGQGALALLATLANLLICLWMFGHEVLLSLPWAGFGMLFELRLYHFSAFILAAAA
GFALLVSLYSLHFMARRSHTNQFYAYLLISLGMVNGAVLADNLILMLFFWEGLLCTIFGFIVIGSGESFRSAIKAFVLIG
VSDILMMVGIGMTGFLAGTMTISRIHLSPDSALAGAAFLLLVIGATSKAGSMPFHSWIPDAAVDAPLPFMALMPAALEKL
LGIYFLSRITLDLFALSPASWLSPLLMTLGVVTILLAVMMALIQKDYKRLLSYHAISQVGYMILGIGTAVPVGIVGGIFH
MINHAMYKSCLFLTSGSVEAQTGTTDLKKLGGMGMRMPITFICFIIAAAAISGVPPFNGFFSKELIYDGALERHWIFYAG
ALAGSFLTAASFLKLGHAVFLGKAEDEASFSLVREAPLSMLIPMVTIAGGCVLFGVANRLPLDTLIVPIIGPQLAAGHHF
GGFPQNRFLVFMTMLVLFAAWINHLYGVRKSGTGVGASDHIHYSPLLHPVYERAERRLFDPYDLGMKVVQGFSRLAWWID
RANDWLFNVMAVRTAQFCSRSLSALHNGSYATYMAWVAIGLILMGLYLNV

Sequences:

>Translated_610_residues
MTESMMMCAILLPALCGSVALVLPKGGQGALALLATLANLLICLWMFGHEVLLSLPWAGFGMLFELRLYHFSAFILAAAA
GFALLVSLYSLHFMARRSHTNQFYAYLLISLGMVNGAVLADNLILMLFFWEGLLCTIFGFIVIGSGESFRSAIKAFVLIG
VSDILMMVGIGMTGFLAGTMTISRIHLSPDSALAGAAFLLLVIGATSKAGSMPFHSWIPDAAVDAPLPFMALMPAALEKL
LGIYFLSRITLDLFALSPASWLSPLLMTLGVVTILLAVMMALIQKDYKRLLSYHAISQVGYMILGIGTAVPVGIVGGIFH
MINHAMYKSCLFLTSGSVEAQTGTTDLKKLGGMGMRMPITFICFIIAAAAISGVPPFNGFFSKELIYDGALERHWIFYAG
ALAGSFLTAASFLKLGHAVFLGKAEDEASFSLVREAPLSMLIPMVTIAGGCVLFGVANRLPLDTLIVPIIGPQLAAGHHF
GGFPQNRFLVFMTMLVLFAAWINHLYGVRKSGTGVGASDHIHYSPLLHPVYERAERRLFDPYDLGMKVVQGFSRLAWWID
RANDWLFNVMAVRTAQFCSRSLSALHNGSYATYMAWVAIGLILMGLYLNV
>Mature_609_residues
TESMMMCAILLPALCGSVALVLPKGGQGALALLATLANLLICLWMFGHEVLLSLPWAGFGMLFELRLYHFSAFILAAAAG
FALLVSLYSLHFMARRSHTNQFYAYLLISLGMVNGAVLADNLILMLFFWEGLLCTIFGFIVIGSGESFRSAIKAFVLIGV
SDILMMVGIGMTGFLAGTMTISRIHLSPDSALAGAAFLLLVIGATSKAGSMPFHSWIPDAAVDAPLPFMALMPAALEKLL
GIYFLSRITLDLFALSPASWLSPLLMTLGVVTILLAVMMALIQKDYKRLLSYHAISQVGYMILGIGTAVPVGIVGGIFHM
INHAMYKSCLFLTSGSVEAQTGTTDLKKLGGMGMRMPITFICFIIAAAAISGVPPFNGFFSKELIYDGALERHWIFYAGA
LAGSFLTAASFLKLGHAVFLGKAEDEASFSLVREAPLSMLIPMVTIAGGCVLFGVANRLPLDTLIVPIIGPQLAAGHHFG
GFPQNRFLVFMTMLVLFAAWINHLYGVRKSGTGVGASDHIHYSPLLHPVYERAERRLFDPYDLGMKVVQGFSRLAWWIDR
ANDWLFNVMAVRTAQFCSRSLSALHNGSYATYMAWVAIGLILMGLYLNV

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=403, Percent_Identity=26.7990074441687, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI1788614, Length=421, Percent_Identity=31.5914489311164, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788827, Length=422, Percent_Identity=29.6208530805687, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788829, Length=349, Percent_Identity=32.6647564469914, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788831, Length=205, Percent_Identity=34.6341463414634, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI2367154, Length=296, Percent_Identity=33.445945945946, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1788613, Length=344, Percent_Identity=26.453488372093, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 66053; Mature: 65922

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESMMMCAILLPALCGSVALVLPKGGQGALALLATLANLLICLWMFGHEVLLSLPWAGF
CCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
GMLFELRLYHFSAFILAAAAGFALLVSLYSLHFMARRSHTNQFYAYLLISLGMVNGAVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHH
DNLILMLFFWEGLLCTIFGFIVIGSGESFRSAIKAFVLIGVSDILMMVGIGMTGFLAGTM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TISRIHLSPDSALAGAAFLLLVIGATSKAGSMPFHSWIPDAAVDAPLPFMALMPAALEKL
EEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHH
LGIYFLSRITLDLFALSPASWLSPLLMTLGVVTILLAVMMALIQKDYKRLLSYHAISQVG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YMILGIGTAVPVGIVGGIFHMINHAMYKSCLFLTSGSVEAQTGTTDLKKLGGMGMRMPIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCHHHH
FICFIIAAAAISGVPPFNGFFSKELIYDGALERHWIFYAGALAGSFLTAASFLKLGHAVF
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LGKAEDEASFSLVREAPLSMLIPMVTIAGGCVLFGVANRLPLDTLIVPIIGPQLAAGHHF
EECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCC
GGFPQNRFLVFMTMLVLFAAWINHLYGVRKSGTGVGASDHIHYSPLLHPVYERAERRLFD
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PYDLGMKVVQGFSRLAWWIDRANDWLFNVMAVRTAQFCSRSLSALHNGSYATYMAWVAIG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LILMGLYLNV
HHHHHHHHCC
>Mature Secondary Structure 
TESMMMCAILLPALCGSVALVLPKGGQGALALLATLANLLICLWMFGHEVLLSLPWAGF
CCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
GMLFELRLYHFSAFILAAAAGFALLVSLYSLHFMARRSHTNQFYAYLLISLGMVNGAVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHH
DNLILMLFFWEGLLCTIFGFIVIGSGESFRSAIKAFVLIGVSDILMMVGIGMTGFLAGTM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TISRIHLSPDSALAGAAFLLLVIGATSKAGSMPFHSWIPDAAVDAPLPFMALMPAALEKL
EEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHH
LGIYFLSRITLDLFALSPASWLSPLLMTLGVVTILLAVMMALIQKDYKRLLSYHAISQVG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YMILGIGTAVPVGIVGGIFHMINHAMYKSCLFLTSGSVEAQTGTTDLKKLGGMGMRMPIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCHHHH
FICFIIAAAAISGVPPFNGFFSKELIYDGALERHWIFYAGALAGSFLTAASFLKLGHAVF
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LGKAEDEASFSLVREAPLSMLIPMVTIAGGCVLFGVANRLPLDTLIVPIIGPQLAAGHHF
EECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCC
GGFPQNRFLVFMTMLVLFAAWINHLYGVRKSGTGVGASDHIHYSPLLHPVYERAERRLFD
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PYDLGMKVVQGFSRLAWWIDRANDWLFNVMAVRTAQFCSRSLSALHNGSYATYMAWVAIG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LILMGLYLNV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]