Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 118581884

GI number: 118581884

Start: 3847640

End: 3849049

Strand: Direct

Name: gltX

Synonym: Ppro_3484

Alternate gene names: 118581884

Gene position: 3847640-3849049 (Clockwise)

Preceding gene: 118581877

Following gene: 118581885

Centisome position: 96.0

GC content: 64.18

Gene sequence:

>1410_bases
ATGTCCGATATCCGCGTCCGTTTCGCACCCAGCCCCACCGGCTACCTCCACGTGGGAGGAGCCCGCACGGCCCTGTTCAA
CTGGCTCTTGGCCAGGAAGCAGGGGGGCACCTTCATCCTGCGCATCGAGGATACCGATGTGGAGCGTTCCACCCAGGAAT
CGGTGGATGCCATCCTGCAGGGAATGGAGTGGCTGGGGCTTGACTGGGACGAGGGCCCCTTCTACCAGACCGACAACTTC
CCGCTCTACCGGGAGTACATCCAGAAGCTGCTGGACGAGGGGAAGGCCTACCGCTGCTACTGCACGGCCGAGGAACTGGA
GGCAAAGCGCGAACTGGCCATGAAGGAAGGGCGCAAGCCCAAGTATGACGGCACCTGCCGCGACCTGACGGAACAGCCCG
CAGACCGGCCGTTTGTGGTCCGCTTCCGTGCACCCCAGGACGGCGGAGCCACCTCCTTCAACGACCTGATCAAGGGGACC
ATCACCTTCCCCAACGACGAACTGGACGACCTGATCGTCCAGCGCAGCGACGGCACGCCCACCTACAACTTCTGCGTAGT
GATCGACGACGCCATCATGAAGATCACCACCGTCATCCGCGGTGACGACCATGTCAACAACACTCCGCGCCAGGTCCAGC
TCTACCAGGCCCTGGGCTTTCCCGTACCGCAGTTCGCCCACGTCCCCATGATCCTGGGCAGCGACAAGGCGCGCCTCTCC
AAGCGCCACGGCGCCACCAGCGTGATTGCCTACCGCGATATGGGCTTCCTGCCCGAGGCGCTGATGAACTACCTGGTACG
TCTGGGGTGGAGCCACGGCGACGACGAGATCTTCAGCCGCGACGAGATGGTGGCCAAGTTCGACATCTCAAACGTGGGGC
GCTCGCCCAGCGTCTTCAACCCGGACAAGCTGCTCTGGCTGAACGCCCACTACATAAAGCACGGCGACCCGCAACGTCTG
GCCGACCTGCTGGTGCCGTTCCTGAAAATGCGGGGCGTTGATCCGGCCAACGGCGGGCCCCAGCTGGCGGTAGCCATCAA
AACCCTGCAGGAGCGCGCCCGCACCATGCTGGAGATGGCCGATTCGGCCCTGTTCTACTACCACGCCCCCGAATCCTACG
ACAGGACCGCCCTGGCCAAGTTCGAGAAGGAGCACCTGCTGGCGGTGTATGCCACCGTGGCGGAGAAACTCTCCGCTGCC
ACGGCGGTCACGGCAGCCGAATTCGACGCGCTCTTCAAGGAGATCTGCGCCGAAAAGGGGTGGAAGATGCCCCAGGTGGG
CCAACCGGTGCGCATCGCCCTCTCCGGCGGCACCCATGCCCCGGGCATCGGCGAGATCATCGTCACCCTGGGCAAAGAGG
AGACCATCCAACGCATCCAGCGGGCGCGGGAGTTCGTGGAGAAGCAGTAG

Upstream 100 bases:

>100_bases
CAACATGGAGAAAATGCTTTTTGAGACCACCCGTCGGTGCTATAGTGTTCGGTCGCTAATCAAAAGCACGCCTGCATGGC
ACAGAGAGGGAGATACAGTC

Downstream 100 bases:

>100_bases
CAACGCCGCTCTTGTTCGTTCGCGTACACACGTGGTGCGCCGTTGACGGGCACGCCGACGAAAGGGGTAGACAGTGCCAT
GTCCATCTTCACCCTGAAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSDIRVRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVERSTQESVDAILQGMEWLGLDWDEGPFYQTDNF
PLYREYIQKLLDEGKAYRCYCTAEELEAKRELAMKEGRKPKYDGTCRDLTEQPADRPFVVRFRAPQDGGATSFNDLIKGT
ITFPNDELDDLIVQRSDGTPTYNFCVVIDDAIMKITTVIRGDDHVNNTPRQVQLYQALGFPVPQFAHVPMILGSDKARLS
KRHGATSVIAYRDMGFLPEALMNYLVRLGWSHGDDEIFSRDEMVAKFDISNVGRSPSVFNPDKLLWLNAHYIKHGDPQRL
ADLLVPFLKMRGVDPANGGPQLAVAIKTLQERARTMLEMADSALFYYHAPESYDRTALAKFEKEHLLAVYATVAEKLSAA
TAVTAAEFDALFKEICAEKGWKMPQVGQPVRIALSGGTHAPGIGEIIVTLGKEETIQRIQRAREFVEKQ

Sequences:

>Translated_469_residues
MSDIRVRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVERSTQESVDAILQGMEWLGLDWDEGPFYQTDNF
PLYREYIQKLLDEGKAYRCYCTAEELEAKRELAMKEGRKPKYDGTCRDLTEQPADRPFVVRFRAPQDGGATSFNDLIKGT
ITFPNDELDDLIVQRSDGTPTYNFCVVIDDAIMKITTVIRGDDHVNNTPRQVQLYQALGFPVPQFAHVPMILGSDKARLS
KRHGATSVIAYRDMGFLPEALMNYLVRLGWSHGDDEIFSRDEMVAKFDISNVGRSPSVFNPDKLLWLNAHYIKHGDPQRL
ADLLVPFLKMRGVDPANGGPQLAVAIKTLQERARTMLEMADSALFYYHAPESYDRTALAKFEKEHLLAVYATVAEKLSAA
TAVTAAEFDALFKEICAEKGWKMPQVGQPVRIALSGGTHAPGIGEIIVTLGKEETIQRIQRAREFVEKQ
>Mature_468_residues
SDIRVRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVERSTQESVDAILQGMEWLGLDWDEGPFYQTDNFP
LYREYIQKLLDEGKAYRCYCTAEELEAKRELAMKEGRKPKYDGTCRDLTEQPADRPFVVRFRAPQDGGATSFNDLIKGTI
TFPNDELDDLIVQRSDGTPTYNFCVVIDDAIMKITTVIRGDDHVNNTPRQVQLYQALGFPVPQFAHVPMILGSDKARLSK
RHGATSVIAYRDMGFLPEALMNYLVRLGWSHGDDEIFSRDEMVAKFDISNVGRSPSVFNPDKLLWLNAHYIKHGDPQRLA
DLLVPFLKMRGVDPANGGPQLAVAIKTLQERARTMLEMADSALFYYHAPESYDRTALAKFEKEHLLAVYATVAEKLSAAT
AVTAAEFDALFKEICAEKGWKMPQVGQPVRIALSGGTHAPGIGEIIVTLGKEETIQRIQRAREFVEKQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=489, Percent_Identity=29.4478527607362, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI62241042, Length=246, Percent_Identity=27.6422764227642, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=52.1551724137931, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI226510925, Length=244, Percent_Identity=35.2459016393443, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1786895, Length=121, Percent_Identity=31.404958677686, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=484, Percent_Identity=31.198347107438, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324540, Length=492, Percent_Identity=31.9105691056911, Blast_Score=266, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6321192, Length=232, Percent_Identity=27.5862068965517, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=212, Percent_Identity=32.5471698113208, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24665321, Length=490, Percent_Identity=32.4489795918367, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI17864488, Length=299, Percent_Identity=26.0869565217391, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24649466, Length=217, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PELPD (A1AUQ6)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_903134.1
- ProteinModelPortal:   A1AUQ6
- SMR:   A1AUQ6
- STRING:   A1AUQ6
- GeneID:   4572651
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3484
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   A1AUQ6
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52730; Mature: 52598

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIRVRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVERSTQESVDAILQ
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHH
GMEWLGLDWDEGPFYQTDNFPLYREYIQKLLDEGKAYRCYCTAEELEAKRELAMKEGRKP
CHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCC
KYDGTCRDLTEQPADRPFVVRFRAPQDGGATSFNDLIKGTITFPNDELDDLIVQRSDGTP
CCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHEEEEECCCCCHHHHHEECCCCCC
TYNFCVVIDDAIMKITTVIRGDDHVNNTPRQVQLYQALGFPVPQFAHVPMILGSDKARLS
CEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEHHHHHCCCCCCCCCCCEEECCCHHHHH
KRHGATSVIAYRDMGFLPEALMNYLVRLGWSHGDDEIFSRDEMVAKFDISNVGRSPSVFN
HHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHCCCHHEEEEEHHHCCCCCCCCC
PDKLLWLNAHYIKHGDPQRLADLLVPFLKMRGVDPANGGPQLAVAIKTLQERARTMLEMA
CCEEEEEEEHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DSALFYYHAPESYDRTALAKFEKEHLLAVYATVAEKLSAATAVTAAEFDALFKEICAEKG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WKMPQVGQPVRIALSGGTHAPGIGEIIVTLGKEETIQRIQRAREFVEKQ
CCCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDIRVRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVERSTQESVDAILQ
CCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHH
GMEWLGLDWDEGPFYQTDNFPLYREYIQKLLDEGKAYRCYCTAEELEAKRELAMKEGRKP
CHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCC
KYDGTCRDLTEQPADRPFVVRFRAPQDGGATSFNDLIKGTITFPNDELDDLIVQRSDGTP
CCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHEEEEECCCCCHHHHHEECCCCCC
TYNFCVVIDDAIMKITTVIRGDDHVNNTPRQVQLYQALGFPVPQFAHVPMILGSDKARLS
CEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEHHHHHCCCCCCCCCCCEEECCCHHHHH
KRHGATSVIAYRDMGFLPEALMNYLVRLGWSHGDDEIFSRDEMVAKFDISNVGRSPSVFN
HHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHCCCHHEEEEEHHHCCCCCCCCC
PDKLLWLNAHYIKHGDPQRLADLLVPFLKMRGVDPANGGPQLAVAIKTLQERARTMLEMA
CCEEEEEEEHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DSALFYYHAPESYDRTALAKFEKEHLLAVYATVAEKLSAATAVTAAEFDALFKEICAEKG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WKMPQVGQPVRIALSGGTHAPGIGEIIVTLGKEETIQRIQRAREFVEKQ
CCCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA