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The map label for this gene is nreB [H]

Identifier: 118581882

GI number: 118581882

Start: 3844130

End: 3845599

Strand: Reverse

Name: nreB [H]

Synonym: Ppro_3482

Alternate gene names: 118581882

Gene position: 3845599-3844130 (Counterclockwise)

Preceding gene: 118581883

Following gene: 118581881

Centisome position: 95.95

GC content: 62.59

Gene sequence:

>1470_bases
ATGGCGTCCGAACGAACCCTGAGGATGGAGAGTGAACGCAAACGGGGTGAAGATACCTTTCATTTCCTGGCATCGAGGCT
GCCTGACACGGTGGTGGCGCGCTTCGACCGCGCTCTCCGCCATATCTATGTCTCCGACTCCATCGAGGCGGTCACGGGAA
AACCATGGCAATTCTTCATCGGCAGGACCAACCGCGACCTGGGCATGCCGGAAGAGCTGGTGCAGCAGTGGGATGCTGCA
CTGCATTCGGTGTGGGATACCGGACAGGCGCGGCAGATTGAATTCACCTACCCCGCGCCGGACAGCAGGCTGATCTATTA
CGAGTGCCAGCTGATTCCGGAACTGTCCGACAATGGCACGATCAGTTCGGTCGTCAGCATCAGTCGCGATATCAGCGAAC
GTAAGCGGTCCCAACTCCTGCTGCAGCAGAGCGAGGAGAAATTCAGGATGACCTTCGAGGGGGCCTCGGACGCCATCTTC
TGGGCCGACGCCGACAGCGGCATACTCATCAACTGCAACCGGGCCGCCGAAGCGATGCTGGAGCTGCCGCGTGAGGAGAT
CATCGGCAGGCATCAGACGGTTCTGCACCCTCCCGAGATGGCGGAGCATTTCGCCGCCCAGTTCAGGAGGACCGTTGCCC
AGCATAGAGAGGTTGACAACGCCGAGGCGATCGTTGTCTCCAAGAGCGGCAGAAGGATCCCGGTGCACATCAAACACTCC
GTCACCCGGGTGGGAGAGATGAGCATCATGCAAGGGCTGTTCAGGGACATCAGCGAGCGTGTCAGGGCCGAGGAGCAGCT
GGTGGAGCTCAACCAGAAGCTGCGTGCCCTGAGTGATCATCTGCAGACGGTCCAGGAGCGGGAACGGCTCGCCATGGCGC
GCGACATCCATGACGAGATCGGACAGAGTCTGACCGTGCTGAAGTTGGATCTGGAATGGATTGCGCACAGGCTGCCGGCG
AAAGAGAGCGCCGTGCAAGAACGGGTGAACGAGATGCGCTGGAACATCGAGCAGCTGACCTCGGCGGTCCAGCGGATCGC
CGCCGATCTGCGCCCGCCGCTGCTGGACAGCCTGGGGCTCGCCGCCGCCATCGACTGGCATGTGGCTGAATTCAGCAGGC
GCAGCGGCATCGAGTGTTTCGTCATGCTCAACGAGGAGGTCGACCCCCTTGAGCCGCAGCTGGCAACCGCGGTTATGCGC
ATAGTCCAGGAGGGGCTCACCAACGTTCTCCGTCATGCCCGCGCCACGGAGGTCAGCGTCTCGCTCTGCAAACGGGACGG
CCAACTCGTGCTGGAGATTGGGGACAATGGTCGCGGCATCAGGCCGGAGGAGATCGAATCGCCGCAGGCCTACGGCCTGA
TGGGGATGCAGGAGCGTGCCCGGATCTGCCGGGGAGAGCTGACCGTTGGCGGAGCAGAGGGAGGCGGTACGCTGCTGCGC
GTGACGGTTCCGCTGCAGAGCGGGGAGTGA

Upstream 100 bases:

>100_bases
GCGGCGATTCACCCTGCGCGAGGATGAGTTCCCCAAGACAACCACCCGCAAGATCAAGCGCTTCGCCGTGGGGGCCGATA
TTGACACCGGGGAGTAGATC

Downstream 100 bases:

>100_bases
ATGTCGTGAAGAGAATCTTGATTGTTGATGACCACACCATCGTCAGGCAGGGAGTGATCAGGGTCCTGCAGGATATCCTG
GAACAGCCCGTGGAGTTCCA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MASERTLRMESERKRGEDTFHFLASRLPDTVVARFDRALRHIYVSDSIEAVTGKPWQFFIGRTNRDLGMPEELVQQWDAA
LHSVWDTGQARQIEFTYPAPDSRLIYYECQLIPELSDNGTISSVVSISRDISERKRSQLLLQQSEEKFRMTFEGASDAIF
WADADSGILINCNRAAEAMLELPREEIIGRHQTVLHPPEMAEHFAAQFRRTVAQHREVDNAEAIVVSKSGRRIPVHIKHS
VTRVGEMSIMQGLFRDISERVRAEEQLVELNQKLRALSDHLQTVQERERLAMARDIHDEIGQSLTVLKLDLEWIAHRLPA
KESAVQERVNEMRWNIEQLTSAVQRIAADLRPPLLDSLGLAAAIDWHVAEFSRRSGIECFVMLNEEVDPLEPQLATAVMR
IVQEGLTNVLRHARATEVSVSLCKRDGQLVLEIGDNGRGIRPEEIESPQAYGLMGMQERARICRGELTVGGAEGGGTLLR
VTVPLQSGE

Sequences:

>Translated_489_residues
MASERTLRMESERKRGEDTFHFLASRLPDTVVARFDRALRHIYVSDSIEAVTGKPWQFFIGRTNRDLGMPEELVQQWDAA
LHSVWDTGQARQIEFTYPAPDSRLIYYECQLIPELSDNGTISSVVSISRDISERKRSQLLLQQSEEKFRMTFEGASDAIF
WADADSGILINCNRAAEAMLELPREEIIGRHQTVLHPPEMAEHFAAQFRRTVAQHREVDNAEAIVVSKSGRRIPVHIKHS
VTRVGEMSIMQGLFRDISERVRAEEQLVELNQKLRALSDHLQTVQERERLAMARDIHDEIGQSLTVLKLDLEWIAHRLPA
KESAVQERVNEMRWNIEQLTSAVQRIAADLRPPLLDSLGLAAAIDWHVAEFSRRSGIECFVMLNEEVDPLEPQLATAVMR
IVQEGLTNVLRHARATEVSVSLCKRDGQLVLEIGDNGRGIRPEEIESPQAYGLMGMQERARICRGELTVGGAEGGGTLLR
VTVPLQSGE
>Mature_488_residues
ASERTLRMESERKRGEDTFHFLASRLPDTVVARFDRALRHIYVSDSIEAVTGKPWQFFIGRTNRDLGMPEELVQQWDAAL
HSVWDTGQARQIEFTYPAPDSRLIYYECQLIPELSDNGTISSVVSISRDISERKRSQLLLQQSEEKFRMTFEGASDAIFW
ADADSGILINCNRAAEAMLELPREEIIGRHQTVLHPPEMAEHFAAQFRRTVAQHREVDNAEAIVVSKSGRRIPVHIKHSV
TRVGEMSIMQGLFRDISERVRAEEQLVELNQKLRALSDHLQTVQERERLAMARDIHDEIGQSLTVLKLDLEWIAHRLPAK
ESAVQERVNEMRWNIEQLTSAVQRIAADLRPPLLDSLGLAAAIDWHVAEFSRRSGIECFVMLNEEVDPLEPQLATAVMRI
VQEGLTNVLRHARATEVSVSLCKRDGQLVLEIGDNGRGIRPEEIESPQAYGLMGMQERARICRGELTVGGAEGGGTLLRV
TVPLQSGE

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=203, Percent_Identity=30.5418719211823, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788812, Length=207, Percent_Identity=27.536231884058, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082311, Length=277, Percent_Identity=29.9638989169675, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55319; Mature: 55187

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASERTLRMESERKRGEDTFHFLASRLPDTVVARFDRALRHIYVSDSIEAVTGKPWQFFI
CCCCCCCCCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
GRTNRDLGMPEELVQQWDAALHSVWDTGQARQIEFTYPAPDSRLIYYECQLIPELSDNGT
ECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCC
ISSVVSISRDISERKRSQLLLQQSEEKFRMTFEGASDAIFWADADSGILINCNRAAEAML
HHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEECCCCCEEEEEECCCCEEEECCHHHHHHH
ELPREEIIGRHQTVLHPPEMAEHFAAQFRRTVAQHREVDNAEAIVVSKSGRRIPVHIKHS
HCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEHHH
VTRVGEMSIMQGLFRDISERVRAEEQLVELNQKLRALSDHLQTVQERERLAMARDIHDEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQSLTVLKLDLEWIAHRLPAKESAVQERVNEMRWNIEQLTSAVQRIAADLRPPLLDSLGL
CCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHCCH
AAAIDWHVAEFSRRSGIECFVMLNEEVDPLEPQLATAVMRIVQEGLTNVLRHARATEVSV
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLCKRDGQLVLEIGDNGRGIRPEEIESPQAYGLMGMQERARICRGELTVGGAEGGGTLLR
HHHHCCCCEEEEECCCCCCCCCHHCCCCCHHCCCCHHHHHHHHCCCEEECCCCCCCEEEE
VTVPLQSGE
EEEECCCCC
>Mature Secondary Structure 
ASERTLRMESERKRGEDTFHFLASRLPDTVVARFDRALRHIYVSDSIEAVTGKPWQFFI
CCCCCCCCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
GRTNRDLGMPEELVQQWDAALHSVWDTGQARQIEFTYPAPDSRLIYYECQLIPELSDNGT
ECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCC
ISSVVSISRDISERKRSQLLLQQSEEKFRMTFEGASDAIFWADADSGILINCNRAAEAML
HHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEECCCCCEEEEEECCCCEEEECCHHHHHHH
ELPREEIIGRHQTVLHPPEMAEHFAAQFRRTVAQHREVDNAEAIVVSKSGRRIPVHIKHS
HCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEHHH
VTRVGEMSIMQGLFRDISERVRAEEQLVELNQKLRALSDHLQTVQERERLAMARDIHDEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQSLTVLKLDLEWIAHRLPAKESAVQERVNEMRWNIEQLTSAVQRIAADLRPPLLDSLGL
CCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHCCH
AAAIDWHVAEFSRRSGIECFVMLNEEVDPLEPQLATAVMRIVQEGLTNVLRHARATEVSV
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLCKRDGQLVLEIGDNGRGIRPEEIESPQAYGLMGMQERARICRGELTVGGAEGGGTLLR
HHHHCCCCEEEEECCCCCCCCCHHCCCCCHHCCCCHHHHHHHHCCCEEECCCCCCCEEEE
VTVPLQSGE
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA