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The map label for this gene is gacA [H]

Identifier: 118581881

GI number: 118581881

Start: 3843486

End: 3844124

Strand: Reverse

Name: gacA [H]

Synonym: Ppro_3481

Alternate gene names: 118581881

Gene position: 3844124-3843486 (Counterclockwise)

Preceding gene: 118581882

Following gene: 118581880

Centisome position: 95.91

GC content: 60.72

Gene sequence:

>639_bases
GTGAAGAGAATCTTGATTGTTGATGACCACACCATCGTCAGGCAGGGAGTGATCAGGGTCCTGCAGGATATCCTGGAACA
GCCCGTGGAGTTCCATGAGGCCTGCACCGGGAGACAGACCCTGGATATGGCCTGCTCGTGCGAGTACAACCTTGTGCTGC
TCGATATCTCGCTGCCGGACCAAAACGGCCTGAACGTGCTCAAGGCATTGCAGCAGAAGCACCCCCGGCTGCCCGTCATC
ATGGTGAGTACCCACCCCGAGGAGCACTATGCCGTCAGGGCCTTGCGGGCAGGGGCCTCCGGTTATGTCAACAAGGGTGA
CGCGGCGGTTGTGCTGAAGGAGGCGATCGAAAAGGTGCTGGCTGGTCGGCGCTACGTGACCCCCTCCCAGGCCGAGTTGC
TGGCCGATGCCCTCTGCGACGGGGCAGAGCCGGCAGTGCTGCACCATGCCCTCTCGGATCGCGAGTATCAGCTGGCCTGC
ATGATGGCGGCCGGCAAGACGCTGACCGAGATTGCCAGGGAACTCTCCCTGAGCGTGAAAACCGTCAGCACCTACCGCAC
GCGGGTTCTTGAGAAGCTTCACTTGAGGAGCACGGCCGATATCATCAACTACTGCATTCAGAACGGGCTTACCCTGTAG

Upstream 100 bases:

>100_bases
GTGCCCGGATCTGCCGGGGAGAGCTGACCGTTGGCGGAGCAGAGGGAGGCGGTACGCTGCTGCGCGTGACGGTTCCGCTG
CAGAGCGGGGAGTGAATGTC

Downstream 100 bases:

>100_bases
GAATCCTCCTACGTGTTTTTCTCCTCTTTTCCCACCTGTGATAGAGACTTGCACTGATTCACACATGACGACAAATCGTA
TTTAATGAATCCTTTGCTTC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKRILIVDDHTIVRQGVIRVLQDILEQPVEFHEACTGRQTLDMACSCEYNLVLLDISLPDQNGLNVLKALQQKHPRLPVI
MVSTHPEEHYAVRALRAGASGYVNKGDAAVVLKEAIEKVLAGRRYVTPSQAELLADALCDGAEPAVLHHALSDREYQLAC
MMAAGKTLTEIARELSLSVKTVSTYRTRVLEKLHLRSTADIINYCIQNGLTL

Sequences:

>Translated_212_residues
MKRILIVDDHTIVRQGVIRVLQDILEQPVEFHEACTGRQTLDMACSCEYNLVLLDISLPDQNGLNVLKALQQKHPRLPVI
MVSTHPEEHYAVRALRAGASGYVNKGDAAVVLKEAIEKVLAGRRYVTPSQAELLADALCDGAEPAVLHHALSDREYQLAC
MMAAGKTLTEIARELSLSVKTVSTYRTRVLEKLHLRSTADIINYCIQNGLTL
>Mature_212_residues
MKRILIVDDHTIVRQGVIRVLQDILEQPVEFHEACTGRQTLDMACSCEYNLVLLDISLPDQNGLNVLKALQQKHPRLPVI
MVSTHPEEHYAVRALRAGASGYVNKGDAAVVLKEAIEKVLAGRRYVTPSQAELLADALCDGAEPAVLHHALSDREYQLAC
MMAAGKTLTEIARELSLSVKTVSTYRTRVLEKLHLRSTADIINYCIQNGLTL

Specific function: Forms part of a two-component regulatory system gacA/gacA(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL) [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=31.9047619047619, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1786747, Length=201, Percent_Identity=29.3532338308458, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1790102, Length=203, Percent_Identity=33.0049261083744, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1788712, Length=208, Percent_Identity=27.8846153846154, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=29.1866028708134, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=38.6792452830189, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788546, Length=208, Percent_Identity=24.5192307692308, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787473, Length=185, Percent_Identity=31.8918918918919, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23481; Mature: 23481

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILIVDDHTIVRQGVIRVLQDILEQPVEFHEACTGRQTLDMACSCEYNLVLLDISLPD
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCEEEEEEEECCC
QNGLNVLKALQQKHPRLPVIMVSTHPEEHYAVRALRAGASGYVNKGDAAVVLKEAIEKVL
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AGRRYVTPSQAELLADALCDGAEPAVLHHALSDREYQLACMMAAGKTLTEIARELSLSVK
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
TVSTYRTRVLEKLHLRSTADIINYCIQNGLTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRILIVDDHTIVRQGVIRVLQDILEQPVEFHEACTGRQTLDMACSCEYNLVLLDISLPD
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCEEEEEEEECCC
QNGLNVLKALQQKHPRLPVIMVSTHPEEHYAVRALRAGASGYVNKGDAAVVLKEAIEKVL
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AGRRYVTPSQAELLADALCDGAEPAVLHHALSDREYQLACMMAAGKTLTEIARELSLSVK
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
TVSTYRTRVLEKLHLRSTADIINYCIQNGLTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002569 [H]