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The map label for this gene is glnG [H]

Identifier: 118581880

GI number: 118581880

Start: 3842645

End: 3843331

Strand: Reverse

Name: glnG [H]

Synonym: Ppro_3480

Alternate gene names: 118581880

Gene position: 3843331-3842645 (Counterclockwise)

Preceding gene: 118581881

Following gene: 118581879

Centisome position: 95.89

GC content: 55.17

Gene sequence:

>687_bases
TTGATTAGAGCAGCCAAGGGGCTGATTGCTGTCATCGATGATGATCCGCTGGTGTTGCAGTACCTTGATACGGCTCTCTC
CCTGGCCGGGTATCGTGTGCTCCCCTTTTCCAACGGGGAATCTGCCCTCGAGGCCATGGATGCGGTTCATGTTGACGTCT
TCCTGAGCGATGTGTGCATGCCCGGCATGGACGGCCTGGAACTGTTGGAGGCCATTCGCCGGCGGGACAGTGAAGCACCG
GTCATTCTGATAACCGCTTTCCCCGGTTTCGATCTGGCGGTTTCCGCGATCAAGAAAGGAGCCTATGACTTCATCGTCAA
GCCTTTCAAGAACGAGTACCTGCTGCACGCCATCGAGAGGGGTATCAATTATCGACGGCTGAAAAATATCGAGAAGAGCT
ATCGGCAGGAGCTGGAATGGACGGTTGTTCTGCGTTCAGCCGAGCTGGCCGATGCTCTGGGCAAGTTGAAGAGCATGAGC
ATGGAGACGATTTCCCGCCTGACCTCAGCGGCGGAACTGAGGGATGAGGACACGGGGAAACATATCGCACGTATCGGGCT
CTATGCGGAACTGCTGGCGGGTGAATTGGGAATGAACAGCGATTTTGTCGAGACTATCCGCGTTGCTAGGAGTCTGTCGG
GCTTAACGCATTCAGCCGTCATCTCCAGGGCGTTTTCAATTGGTTAA

Upstream 100 bases:

>100_bases
TGATTCACACATGACGACAAATCGTATTTAATGAATCCTTTGCTTCTCCGGAGCGACTGGGCACAAGCCGTCCAGTCGCC
GGACGCCGAGGAGGTCCTGT

Downstream 100 bases:

>100_bases
CCTGCTGTATTTATTGAATAAGTCGTCAGCTTTAGCTTGCGTTTTCTTGGTGTATCCCGTATATTTGTCCGGTAATATCA
ACTGGTTAGGTATCGGGGTT

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MIRAAKGLIAVIDDDPLVLQYLDTALSLAGYRVLPFSNGESALEAMDAVHVDVFLSDVCMPGMDGLELLEAIRRRDSEAP
VILITAFPGFDLAVSAIKKGAYDFIVKPFKNEYLLHAIERGINYRRLKNIEKSYRQELEWTVVLRSAELADALGKLKSMS
METISRLTSAAELRDEDTGKHIARIGLYAELLAGELGMNSDFVETIRVARSLSGLTHSAVISRAFSIG

Sequences:

>Translated_228_residues
MIRAAKGLIAVIDDDPLVLQYLDTALSLAGYRVLPFSNGESALEAMDAVHVDVFLSDVCMPGMDGLELLEAIRRRDSEAP
VILITAFPGFDLAVSAIKKGAYDFIVKPFKNEYLLHAIERGINYRRLKNIEKSYRQELEWTVVLRSAELADALGKLKSMS
METISRLTSAAELRDEDTGKHIARIGLYAELLAGELGMNSDFVETIRVARSLSGLTHSAVISRAFSIG
>Mature_228_residues
MIRAAKGLIAVIDDDPLVLQYLDTALSLAGYRVLPFSNGESALEAMDAVHVDVFLSDVCMPGMDGLELLEAIRRRDSEAP
VILITAFPGFDLAVSAIKKGAYDFIVKPFKNEYLLHAIERGINYRRLKNIEKSYRQELEWTVVLRSAELADALGKLKSMS
METISRLTSAAELRDEDTGKHIARIGLYAELLAGELGMNSDFVETIRVARSLSGLTHSAVISRAFSIG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=168, Percent_Identity=35.1190476190476, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788550, Length=143, Percent_Identity=32.8671328671329, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=33.6206896551724, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789402, Length=101, Percent_Identity=33.6633663366337, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788905, Length=109, Percent_Identity=33.9449541284404, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=161, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=121, Percent_Identity=33.0578512396694, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 25091; Mature: 25091

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRAAKGLIAVIDDDPLVLQYLDTALSLAGYRVLPFSNGESALEAMDAVHVDVFLSDVCM
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
PGMDGLELLEAIRRRDSEAPVILITAFPGFDLAVSAIKKGAYDFIVKPFKNEYLLHAIER
CCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHH
GINYRRLKNIEKSYRQELEWTVVLRSAELADALGKLKSMSMETISRLTSAAELRDEDTGK
CCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
HIARIGLYAELLAGELGMNSDFVETIRVARSLSGLTHSAVISRAFSIG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIRAAKGLIAVIDDDPLVLQYLDTALSLAGYRVLPFSNGESALEAMDAVHVDVFLSDVCM
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
PGMDGLELLEAIRRRDSEAPVILITAFPGFDLAVSAIKKGAYDFIVKPFKNEYLLHAIER
CCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHH
GINYRRLKNIEKSYRQELEWTVVLRSAELADALGKLKSMSMETISRLTSAAELRDEDTGK
CCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
HIARIGLYAELLAGELGMNSDFVETIRVARSLSGLTHSAVISRAFSIG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]