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The map label for this gene is hemD [H]

Identifier: 118581774

GI number: 118581774

Start: 3725531

End: 3727060

Strand: Reverse

Name: hemD [H]

Synonym: Ppro_3374

Alternate gene names: 118581774

Gene position: 3727060-3725531 (Counterclockwise)

Preceding gene: 118581775

Following gene: 118581773

Centisome position: 92.99

GC content: 65.88

Gene sequence:

>1530_bases
GTGAAATCTGAAACGAGAGGAATAGTATATCTTGTGGGATCCGGCCCCGGCGATCCGGGACTGATCACCCTGCGCGGCAT
GGAGTGCCTGCGCCAGGCAGAGGTGGTGGTGTACGACTACCTGGCCAACGAACTGCTCCTCAACCATGCCCCGGAATCGG
CGGAGCGGATCTACGCCGGAAAGATCGGCGGTCGCCATAACCAGGGGCAGGATGAGATCAATGCCCTGCTGGTGCGGAAG
GGGAGTGAGGGGAAGATCGTGGTTCGCCTCAAGGGGGGCGATCCCTTCGTGTTCGGTCGGGGAGGAGAGGAGTGCGAGGC
TCTGCGCGAGGCGGGGATTCCCTTCCAGGTCGTGCCGGGAGTCACCGCGGCCGTGGGCGCTGCGGCCTACGCCGGCATTC
CCCTCACCCACCGCGAGTACACCGCCTCGGTCACGCTGGTCGCCGGCCAGGAGGGGAAGGACAAGGACGAGTCCAACATC
GACTGGGAGCGCCTCTCCCACGGCAACGGCACGGTTGTCTTCTACATGGGGGTCACCACCCTGCGTCGCAACATGGAGCG
CCTGATACAGCACGGCAAGAGCGCCGACACGCCGGTGGCCCTGGTGCGTTGGGGCACCACGCCGAAGCAGCAGGTGCTGA
CCGGCACCCTGGCGGATATCGCCGACCGGGCCGAGAAGAGCGCCTTCAAGCCGCCGGCGGTAACCGTGGTGGGTGACGTG
GTCAGGCTGAGGGACAAGCTGGCCTGGTTCGACGGCCGTCCGCTCTTCGGCAAGAGGATCATGGTCACCCGCGCCGCCGT
CCAGGCGGGGGAGTTTGCCGGCATGCTGGCCCAGCGGGGAGCGGCGGTGATCGAATGTCCCACCATCCGCCTGGTGGAAC
CGGAACAGTGGCAGCCGCTGGACGCGGCCCTGCGCGACATCTCCGGCTATGACTGGATTGTGCTCACCTCGGCCAACGCG
GTGCGCTGCTTCTTCAGGCGCCTGGACGCCCTTGGCCTGGACGCCCGTAGCCTGGCCGGTTGTCGGGTCTGCGCCGTGGG
TCCGAAAACCGCCGCAAAGATCAGGAACTTTGGCATCAATGCCGATCTGGTCTCCTCCGACTACAAGGCCGAGGGAGTGG
TAGCCGAATTTGCCAAGCTGGACATCCGCGGCAAGAAGATCCTCTACCCCTGCGCGGACCGGGCCCGCGACCTGATTCCC
CGGGAGCTGGGCACCATGGGCGCCCAGGTCGATGCCCCCATCGCCTACCGCACGGTCCTTCCGGACAGGCTGCCGCCCGA
GGCGGTGTTTGCCCTGGAGAAGCGCTCCGTGGACTGCATCACCTTCACCTCATCCTCCACGGTGCAGAATCTTGCCAGCA
TGCTGGGCGAGGACCGCATGTTGGACATGCTCAAGGGAGTGGCCATCGCTTCCATCGGACCGATCACCACGCGCAGCTGC
CGCGCCCTGGGACTCAAGGTGGATATCGAGCCTGCCGAATACACCCTGGATGCCCTGACCGACGCCATCGAAGCCTTCTT
GACGGCCTGA

Upstream 100 bases:

>100_bases
ATCAGCGGTCCGGTTGATCAATGTGAGCACCTGGGGGAGGAACTGGCCGACCGCCTGCTCTCCCGGGGGGGCAGGGCAAT
TCTGGAGGAGGTTTACCAGC

Downstream 100 bases:

>100_bases
TGGAGCTGGGTTATAGAGTAAATGGCTGAGAACAATCTTGAGGAAGGGAGACATATGCAGCCTGTCCAGGCACTGATATC
CGGCAAGGTGCGCCTCCCTT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKSETRGIVYLVGSGPGDPGLITLRGMECLRQAEVVVYDYLANELLLNHAPESAERIYAGKIGGRHNQGQDEINALLVRK
GSEGKIVVRLKGGDPFVFGRGGEECEALREAGIPFQVVPGVTAAVGAAAYAGIPLTHREYTASVTLVAGQEGKDKDESNI
DWERLSHGNGTVVFYMGVTTLRRNMERLIQHGKSADTPVALVRWGTTPKQQVLTGTLADIADRAEKSAFKPPAVTVVGDV
VRLRDKLAWFDGRPLFGKRIMVTRAAVQAGEFAGMLAQRGAAVIECPTIRLVEPEQWQPLDAALRDISGYDWIVLTSANA
VRCFFRRLDALGLDARSLAGCRVCAVGPKTAAKIRNFGINADLVSSDYKAEGVVAEFAKLDIRGKKILYPCADRARDLIP
RELGTMGAQVDAPIAYRTVLPDRLPPEAVFALEKRSVDCITFTSSSTVQNLASMLGEDRMLDMLKGVAIASIGPITTRSC
RALGLKVDIEPAEYTLDALTDAIEAFLTA

Sequences:

>Translated_509_residues
MKSETRGIVYLVGSGPGDPGLITLRGMECLRQAEVVVYDYLANELLLNHAPESAERIYAGKIGGRHNQGQDEINALLVRK
GSEGKIVVRLKGGDPFVFGRGGEECEALREAGIPFQVVPGVTAAVGAAAYAGIPLTHREYTASVTLVAGQEGKDKDESNI
DWERLSHGNGTVVFYMGVTTLRRNMERLIQHGKSADTPVALVRWGTTPKQQVLTGTLADIADRAEKSAFKPPAVTVVGDV
VRLRDKLAWFDGRPLFGKRIMVTRAAVQAGEFAGMLAQRGAAVIECPTIRLVEPEQWQPLDAALRDISGYDWIVLTSANA
VRCFFRRLDALGLDARSLAGCRVCAVGPKTAAKIRNFGINADLVSSDYKAEGVVAEFAKLDIRGKKILYPCADRARDLIP
RELGTMGAQVDAPIAYRTVLPDRLPPEAVFALEKRSVDCITFTSSSTVQNLASMLGEDRMLDMLKGVAIASIGPITTRSC
RALGLKVDIEPAEYTLDALTDAIEAFLTA
>Mature_509_residues
MKSETRGIVYLVGSGPGDPGLITLRGMECLRQAEVVVYDYLANELLLNHAPESAERIYAGKIGGRHNQGQDEINALLVRK
GSEGKIVVRLKGGDPFVFGRGGEECEALREAGIPFQVVPGVTAAVGAAAYAGIPLTHREYTASVTLVAGQEGKDKDESNI
DWERLSHGNGTVVFYMGVTTLRRNMERLIQHGKSADTPVALVRWGTTPKQQVLTGTLADIADRAEKSAFKPPAVTVVGDV
VRLRDKLAWFDGRPLFGKRIMVTRAAVQAGEFAGMLAQRGAAVIECPTIRLVEPEQWQPLDAALRDISGYDWIVLTSANA
VRCFFRRLDALGLDARSLAGCRVCAVGPKTAAKIRNFGINADLVSSDYKAEGVVAEFAKLDIRGKKILYPCADRARDLIP
RELGTMGAQVDAPIAYRTVLPDRLPPEAVFALEKRSVDCITFTSSSTVQNLASMLGEDRMLDMLKGVAIASIGPITTRSC
RALGLKVDIEPAEYTLDALTDAIEAFLTA

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=247, Percent_Identity=49.3927125506073, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322922, Length=236, Percent_Identity=32.2033898305085, Blast_Score=116, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54969; Mature: 54969

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSETRGIVYLVGSGPGDPGLITLRGMECLRQAEVVVYDYLANELLLNHAPESAERIYAG
CCCCCCEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEE
KIGGRHNQGQDEINALLVRKGSEGKIVVRLKGGDPFVFGRGGEECEALREAGIPFQVVPG
CCCCCCCCCHHHHEEEEEEECCCCEEEEEEECCCEEEECCCCHHHHHHHHCCCCEEECCC
VTAAVGAAAYAGIPLTHREYTASVTLVAGQEGKDKDESNIDWERLSHGNGTVVFYMGVTT
CHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHCCCCCEEEEEECHHH
LRRNMERLIQHGKSADTPVALVRWGTTPKQQVLTGTLADIADRAEKSAFKPPAVTVVGDV
HHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VRLRDKLAWFDGRPLFGKRIMVTRAAVQAGEFAGMLAQRGAAVIECPTIRLVEPEQWQPL
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCH
DAALRDISGYDWIVLTSANAVRCFFRRLDALGLDARSLAGCRVCAVGPKTAAKIRNFGIN
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHCCCEEEECCCHHHHHHHHCCCC
ADLVSSDYKAEGVVAEFAKLDIRGKKILYPCADRARDLIPRELGTMGAQVDAPIAYRTVL
CEEECCCCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEECC
PDRLPPEAVFALEKRSVDCITFTSSSTVQNLASMLGEDRMLDMLKGVAIASIGPITTRSC
CCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHCHHHCCCCCCCCCCH
RALGLKVDIEPAEYTLDALTDAIEAFLTA
HEEEEEEECCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSETRGIVYLVGSGPGDPGLITLRGMECLRQAEVVVYDYLANELLLNHAPESAERIYAG
CCCCCCEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEE
KIGGRHNQGQDEINALLVRKGSEGKIVVRLKGGDPFVFGRGGEECEALREAGIPFQVVPG
CCCCCCCCCHHHHEEEEEEECCCCEEEEEEECCCEEEECCCCHHHHHHHHCCCCEEECCC
VTAAVGAAAYAGIPLTHREYTASVTLVAGQEGKDKDESNIDWERLSHGNGTVVFYMGVTT
CHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHCCCCCEEEEEECHHH
LRRNMERLIQHGKSADTPVALVRWGTTPKQQVLTGTLADIADRAEKSAFKPPAVTVVGDV
HHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VRLRDKLAWFDGRPLFGKRIMVTRAAVQAGEFAGMLAQRGAAVIECPTIRLVEPEQWQPL
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCH
DAALRDISGYDWIVLTSANAVRCFFRRLDALGLDARSLAGCRVCAVGPKTAAKIRNFGIN
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHCCCEEEECCCHHHHHHHHCCCC
ADLVSSDYKAEGVVAEFAKLDIRGKKILYPCADRARDLIPRELGTMGAQVDAPIAYRTVL
CEEECCCCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEECC
PDRLPPEAVFALEKRSVDCITFTSSSTVQNLASMLGEDRMLDMLKGVAIASIGPITTRSC
CCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHCHHHCCCCCCCCCCH
RALGLKVDIEPAEYTLDALTDAIEAFLTA
HEEEEEEECCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]