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The map label for this gene is pleD [H]

Identifier: 118581773

GI number: 118581773

Start: 3723959

End: 3725476

Strand: Reverse

Name: pleD [H]

Synonym: Ppro_3373

Alternate gene names: 118581773

Gene position: 3725476-3723959 (Counterclockwise)

Preceding gene: 118581774

Following gene: 118581772

Centisome position: 92.95

GC content: 60.54

Gene sequence:

>1518_bases
ATGCAGCCTGTCCAGGCACTGATATCCGGCAAGGTGCGCCTCCCTTCCCCGCCACTGATTGCCGTCAAGATTCTGGAAGA
GATTCGCAGGGATGACTTTACCTTTCAGGACCTGTCCTACCTGATCGAGTCCGATCCGGCCCTGACGGCGCGGGTGCTTA
AGGCGGCGAACTCATCGTTCTTCTGCCCCGGCAACAGGGTGACCAGCATCGAGCGGGCCCTGGCCCTGCTGGGATCGAAC
GCCGTCACCAACATCGCCCTCTCCTTTGTCATCGTCACCGGATTTCAGCCCGGATCGACGGGGAGCTTCGACACCACCAC
CTTCTGGCGGCGTGCGTTGACCACGGCCGTTGCCGCGGAACTGATCTCCACGCTGGTGGGGAGTCAGGACCGCGACATCT
TCGTCATCGCCCTGCTCCAGGACATCGGCATCCTGGTCATGCACGGCAATGACCCCCAGGAGTACCAGAGAGTCTTTCAG
TTGAGAACGGAACAGGGGATGGGGCTGTGCGAGGCTGAACGCCAGATATTCGGCTTCAGTCACCAGGAGCTGGGGGCGGC
TCTGCTGAAGAGCTGGCAGATTCCCGAGGAGATGTACCTGCCCATCAAGTATCACCATGCAACTGATGCCGTTCCCGACC
AGTACCGGCAACAGCACGACATCCTCTGCGTCGCCGACTGTCTGGCCTCCTTTCATTGCGGAACCCAGGACGCGGACAAG
ATCAGGCGCGGCAGACACATCCTCTATGAGACCTTCGGCCTGCTGGGAAAAGAGGTCGAGGCACTAATCGAGGCGGTTGG
CACCAGGTCTTTCGAGGTGCTGGCCTTCTTCGAGATCGCGCCGGACGCCATGCGGCCGCTGGAACTGATCCTCCAGGAGG
CCAACGAGCAGCTGAGCAGCCTGCACGATGCCTATGACCTGCAGGCCATCGAACTGCAACAGGCCAGGGAGAAGGCTGAA
AAACGAGTCCGGGAGCTGCACGACACCAACGTCATGCATCGTGAACTGGCCTTCCGGGACGGCCTGACCGGGGTCTACAA
TCGCCGCTTCTTCCAGGAAGCCCTGGACAAGGAGCTGATGCGGGCCCAGCGCTATAACCGCCAGTTCTCGCTGGTAATCT
TCGATCTGGACAACTTCAAGACCGTCAACGACACCCACGGCCACACCGTCGGCGACCTGCTGCTGATCAACGTCAGCAAG
ACCGTGCAGGATATCCTGCGCACGACCGATATCTTCGCCCGCTACGGTGGAGACGAGTTTGTCATCGTCATGCCCGAAAC
CGACTTGTCCAGCGCCATAGCCGTGGCCGAGAACCTGCGCGCCCGGATCCAGGGGCTGACCACCAGCGTGGGTAACGTCT
TCATCAGGACCACCATCAGCATCGGCCTGACGAGCTACATCGCCACCCTGGGTAACAGGAGCCGCGAGCAGATCATCGAC
ATCGCCGACAAGGCGCTGTACATAGCCAAACACAGCGGGAAAAACAGCACCCGAGCCCTGAAGTTTCCCGATAAATAG

Upstream 100 bases:

>100_bases
CCTGGATGCCCTGACCGACGCCATCGAAGCCTTCTTGACGGCCTGATGGAGCTGGGTTATAGAGTAAATGGCTGAGAACA
ATCTTGAGGAAGGGAGACAT

Downstream 100 bases:

>100_bases
CCGTCCTGTCGCAAAAAGCGCTGCCGTCCATGGGGAGAGGCGCCGTGAATCCTTTTATACCAGGGTCTCAGGGCGCTTTT
CTTTTGCCTCATTCCGCGGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MQPVQALISGKVRLPSPPLIAVKILEEIRRDDFTFQDLSYLIESDPALTARVLKAANSSFFCPGNRVTSIERALALLGSN
AVTNIALSFVIVTGFQPGSTGSFDTTTFWRRALTTAVAAELISTLVGSQDRDIFVIALLQDIGILVMHGNDPQEYQRVFQ
LRTEQGMGLCEAERQIFGFSHQELGAALLKSWQIPEEMYLPIKYHHATDAVPDQYRQQHDILCVADCLASFHCGTQDADK
IRRGRHILYETFGLLGKEVEALIEAVGTRSFEVLAFFEIAPDAMRPLELILQEANEQLSSLHDAYDLQAIELQQAREKAE
KRVRELHDTNVMHRELAFRDGLTGVYNRRFFQEALDKELMRAQRYNRQFSLVIFDLDNFKTVNDTHGHTVGDLLLINVSK
TVQDILRTTDIFARYGGDEFVIVMPETDLSSAIAVAENLRARIQGLTTSVGNVFIRTTISIGLTSYIATLGNRSREQIID
IADKALYIAKHSGKNSTRALKFPDK

Sequences:

>Translated_505_residues
MQPVQALISGKVRLPSPPLIAVKILEEIRRDDFTFQDLSYLIESDPALTARVLKAANSSFFCPGNRVTSIERALALLGSN
AVTNIALSFVIVTGFQPGSTGSFDTTTFWRRALTTAVAAELISTLVGSQDRDIFVIALLQDIGILVMHGNDPQEYQRVFQ
LRTEQGMGLCEAERQIFGFSHQELGAALLKSWQIPEEMYLPIKYHHATDAVPDQYRQQHDILCVADCLASFHCGTQDADK
IRRGRHILYETFGLLGKEVEALIEAVGTRSFEVLAFFEIAPDAMRPLELILQEANEQLSSLHDAYDLQAIELQQAREKAE
KRVRELHDTNVMHRELAFRDGLTGVYNRRFFQEALDKELMRAQRYNRQFSLVIFDLDNFKTVNDTHGHTVGDLLLINVSK
TVQDILRTTDIFARYGGDEFVIVMPETDLSSAIAVAENLRARIQGLTTSVGNVFIRTTISIGLTSYIATLGNRSREQIID
IADKALYIAKHSGKNSTRALKFPDK
>Mature_505_residues
MQPVQALISGKVRLPSPPLIAVKILEEIRRDDFTFQDLSYLIESDPALTARVLKAANSSFFCPGNRVTSIERALALLGSN
AVTNIALSFVIVTGFQPGSTGSFDTTTFWRRALTTAVAAELISTLVGSQDRDIFVIALLQDIGILVMHGNDPQEYQRVFQ
LRTEQGMGLCEAERQIFGFSHQELGAALLKSWQIPEEMYLPIKYHHATDAVPDQYRQQHDILCVADCLASFHCGTQDADK
IRRGRHILYETFGLLGKEVEALIEAVGTRSFEVLAFFEIAPDAMRPLELILQEANEQLSSLHDAYDLQAIELQQAREKAE
KRVRELHDTNVMHRELAFRDGLTGVYNRRFFQEALDKELMRAQRYNRQFSLVIFDLDNFKTVNDTHGHTVGDLLLINVSK
TVQDILRTTDIFARYGGDEFVIVMPETDLSSAIAVAENLRARIQGLTTSVGNVFIRTTISIGLTSYIATLGNRSREQIID
IADKALYIAKHSGKNSTRALKFPDK

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=38.6503067484663, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=38.5542168674699, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=34.3558282208589, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=34.375, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=35.4037267080745, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=29.7142857142857, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=28.3333333333333, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=127, Percent_Identity=35.4330708661417, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=28.8461538461538, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=184, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=28.4810126582279, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=30.188679245283, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 56653; Mature: 56653

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPVQALISGKVRLPSPPLIAVKILEEIRRDDFTFQDLSYLIESDPALTARVLKAANSSF
CCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCE
FCPGNRVTSIERALALLGSNAVTNIALSFVIVTGFQPGSTGSFDTTTFWRRALTTAVAAE
ECCCCCHHHHHHHHHHHCCCHHHHHHEEEHEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
LISTLVGSQDRDIFVIALLQDIGILVMHGNDPQEYQRVFQLRTEQGMGLCEAERQIFGFS
HHHHHHCCCCCCEEEEEEEHHCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
HQELGAALLKSWQIPEEMYLPIKYHHATDAVPDQYRQQHDILCVADCLASFHCGTQDADK
HHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHH
IRRGRHILYETFGLLGKEVEALIEAVGTRSFEVLAFFEIAPDAMRPLELILQEANEQLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LHDAYDLQAIELQQAREKAEKRVRELHDTNVMHRELAFRDGLTGVYNRRFFQEALDKELM
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RAQRYNRQFSLVIFDLDNFKTVNDTHGHTVGDLLLINVSKTVQDILRTTDIFARYGGDEF
HHHHHCCEEEEEEEECCCCCCCCCCCCCEECCEEEEECHHHHHHHHHHHHHHHHCCCCEE
VIVMPETDLSSAIAVAENLRARIQGLTTSVGNVFIRTTISIGLTSYIATLGNRSREQIID
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCHHHHHH
IADKALYIAKHSGKNSTRALKFPDK
HHHHHEEEEECCCCCCCEEECCCCC
>Mature Secondary Structure
MQPVQALISGKVRLPSPPLIAVKILEEIRRDDFTFQDLSYLIESDPALTARVLKAANSSF
CCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCE
FCPGNRVTSIERALALLGSNAVTNIALSFVIVTGFQPGSTGSFDTTTFWRRALTTAVAAE
ECCCCCHHHHHHHHHHHCCCHHHHHHEEEHEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
LISTLVGSQDRDIFVIALLQDIGILVMHGNDPQEYQRVFQLRTEQGMGLCEAERQIFGFS
HHHHHHCCCCCCEEEEEEEHHCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
HQELGAALLKSWQIPEEMYLPIKYHHATDAVPDQYRQQHDILCVADCLASFHCGTQDADK
HHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHH
IRRGRHILYETFGLLGKEVEALIEAVGTRSFEVLAFFEIAPDAMRPLELILQEANEQLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LHDAYDLQAIELQQAREKAEKRVRELHDTNVMHRELAFRDGLTGVYNRRFFQEALDKELM
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RAQRYNRQFSLVIFDLDNFKTVNDTHGHTVGDLLLINVSKTVQDILRTTDIFARYGGDEF
HHHHHCCEEEEEEEECCCCCCCCCCCCCEECCEEEEECHHHHHHHHHHHHHHHHCCCCEE
VIVMPETDLSSAIAVAENLRARIQGLTTSVGNVFIRTTISIGLTSYIATLGNRSREQIID
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCHHHHHH
IADKALYIAKHSGKNSTRALKFPDK
HHHHHEEEEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA