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The map label for this gene is murE [H]

Identifier: 118581694

GI number: 118581694

Start: 3631007

End: 3632617

Strand: Reverse

Name: murE [H]

Synonym: Ppro_3294

Alternate gene names: 118581694

Gene position: 3632617-3631007 (Counterclockwise)

Preceding gene: 118581695

Following gene: 118581693

Centisome position: 90.63

GC content: 64.56

Gene sequence:

>1611_bases
ATGGATCAGGTTCGTACCCTCGGCATGAGGGAGCCTGTCCGCCCGTACTGCACCCCTTCGGGAGACCGTATGAAGCTTAC
CCAGTTGCTGGCAGCGCTTGACAGCCATGACCTTCATGGCGATGGCGCAGTGGAGATCAAGGCCCTGACCTGCGACTCGC
GTGGCATTGTCCCGGATTCGCTCTTCTTTGCCCTGCGGGGGGCCGCCATGGACGGCCACGACTTCATACCGGCGGCCGTG
CAGGCCGGCGCCGTCGCGGTGGTGCTGGAGGACGAGAGCCGTGCGCCTGCCGCCATTCCCTGGGTCAGGGTCAGGGACGG
GCGCAGCGCCATGGCCTGGATGGCGGCGGAATTCCACGGCAACCCCACGTCCCGTCTGCCGCTGATCGGCATAACCGGCA
CCAACGGCAAGACCACCACCACCTATCTGATCGAGGCGATCCTCCGTGCGGCCGGTATTCCGGCGGCGGTGCTGGGCACC
ATCAGCTACCGCTTTGGCGACACGGTCATCACCGCTTCCCACACCACACCGGAATCCACCGAGTTGCAGGCCGCCTTCAC
GCAACTGGCCGCAGCCGGCGCCAAGGCCTTTGTGATGGAGGTCTCCTCCCATGCCCTGGAGCAGAAGCGGGTGGACGGCT
GCCACTTCGATGTGGGTATTTTCAGCAACCTGACCCGCGATCACCTGGACTACCATGTCACCATGGAGAGCTACCGGGAA
GCCAAACTGCGCCTGTTCAGCGAGCTTCTGAAACCGACGGCGGATAAGCCGTTGCGCCGTGCGGCCATCAACATGGACGA
CGAACAGGGTGCAACGGTTGCCGCCCGGGCCGCCTGCCCTGTCATCGGCTACGGAATTTGCCGGGGCGATGTGCGGGCCG
AGGAACTCTCCCTGAGTGTTGACGGCATAAGGGCCAGGCTGGTCACCCCCAAAGGCTCCATCGACGTATCATCGCGCCTG
CTGGGGCGCTTCAACCTCTCCAACATCCTGGCGGCTGTGTCCGCCGGCATTGCCCTGGATCTGCCGCTGGAGGCGATCCG
AGCCGGCATCGAAGGGCATGCCACCGTGCCGGGGCGCATGGAGCGGGTGGACAATCGCTGCGGGATCACGCTGCTGGTGG
ACTATGCCCACACCGGCGATGCCCTGGAGAACGTGCTGACCACCCTGAAGGAACTGGCACAGGGGCGTATCATCACCGTT
TTCGGCTGCGGCGGCGACCGAGATCCGGGTAAACGCCCGATCATGGGGAGGATCGCTGCCAGCATGAGCGACCTGGCCAT
CGTCACCTCGGACAACCCCAGAACTGAGGATCCCTTCTCCATCCTGGAGCAGATCAGGGGAGGCATTACCCCTCTGGGGA
TCAGGGAGTACGGCGTGGATGAACTGGCGGACCGCTCAGGGAAGGGGTTGGCCGAAGGCGCTGATAAGGGGTTCGTGTCG
CTGGAGAACCGTCGCGAGGCCATCAGCCTGGCGGTGCGTCTGGCGCGACCGGGGGATATCGTGCTCCTGGCCGGCAAGGG
ACATGAGGATTACCAGATCATCGGCAGGACGAAGCATCACTTCGATGATCGCGAAGAGGCCAGGCTGGCGCTGGCGGAAA
AGGAAGGGTAG

Upstream 100 bases:

>100_bases
TCATGGAAAAACGGAACATCAACATCAGGCTGTTGGGCAGCGGCCGCGCCATGGAACAGAACCCCCCTCCGGGGCAGCCT
ATCAGGAGTGTTGACGAAGT

Downstream 100 bases:

>100_bases
ATGTTCAGCATTGCGGAGATAGCCGCTGCCACCGCGGGCGGCTTCCAGGGTGACCGGTCGGCCCGGGTGAGCGGGGTCTC
CACCGATTCGCGCACGGTCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MDQVRTLGMREPVRPYCTPSGDRMKLTQLLAALDSHDLHGDGAVEIKALTCDSRGIVPDSLFFALRGAAMDGHDFIPAAV
QAGAVAVVLEDESRAPAAIPWVRVRDGRSAMAWMAAEFHGNPTSRLPLIGITGTNGKTTTTYLIEAILRAAGIPAAVLGT
ISYRFGDTVITASHTTPESTELQAAFTQLAAAGAKAFVMEVSSHALEQKRVDGCHFDVGIFSNLTRDHLDYHVTMESYRE
AKLRLFSELLKPTADKPLRRAAINMDDEQGATVAARAACPVIGYGICRGDVRAEELSLSVDGIRARLVTPKGSIDVSSRL
LGRFNLSNILAAVSAGIALDLPLEAIRAGIEGHATVPGRMERVDNRCGITLLVDYAHTGDALENVLTTLKELAQGRIITV
FGCGGDRDPGKRPIMGRIAASMSDLAIVTSDNPRTEDPFSILEQIRGGITPLGIREYGVDELADRSGKGLAEGADKGFVS
LENRREAISLAVRLARPGDIVLLAGKGHEDYQIIGRTKHHFDDREEARLALAEKEG

Sequences:

>Translated_536_residues
MDQVRTLGMREPVRPYCTPSGDRMKLTQLLAALDSHDLHGDGAVEIKALTCDSRGIVPDSLFFALRGAAMDGHDFIPAAV
QAGAVAVVLEDESRAPAAIPWVRVRDGRSAMAWMAAEFHGNPTSRLPLIGITGTNGKTTTTYLIEAILRAAGIPAAVLGT
ISYRFGDTVITASHTTPESTELQAAFTQLAAAGAKAFVMEVSSHALEQKRVDGCHFDVGIFSNLTRDHLDYHVTMESYRE
AKLRLFSELLKPTADKPLRRAAINMDDEQGATVAARAACPVIGYGICRGDVRAEELSLSVDGIRARLVTPKGSIDVSSRL
LGRFNLSNILAAVSAGIALDLPLEAIRAGIEGHATVPGRMERVDNRCGITLLVDYAHTGDALENVLTTLKELAQGRIITV
FGCGGDRDPGKRPIMGRIAASMSDLAIVTSDNPRTEDPFSILEQIRGGITPLGIREYGVDELADRSGKGLAEGADKGFVS
LENRREAISLAVRLARPGDIVLLAGKGHEDYQIIGRTKHHFDDREEARLALAEKEG
>Mature_536_residues
MDQVRTLGMREPVRPYCTPSGDRMKLTQLLAALDSHDLHGDGAVEIKALTCDSRGIVPDSLFFALRGAAMDGHDFIPAAV
QAGAVAVVLEDESRAPAAIPWVRVRDGRSAMAWMAAEFHGNPTSRLPLIGITGTNGKTTTTYLIEAILRAAGIPAAVLGT
ISYRFGDTVITASHTTPESTELQAAFTQLAAAGAKAFVMEVSSHALEQKRVDGCHFDVGIFSNLTRDHLDYHVTMESYRE
AKLRLFSELLKPTADKPLRRAAINMDDEQGATVAARAACPVIGYGICRGDVRAEELSLSVDGIRARLVTPKGSIDVSSRL
LGRFNLSNILAAVSAGIALDLPLEAIRAGIEGHATVPGRMERVDNRCGITLLVDYAHTGDALENVLTTLKELAQGRIITV
FGCGGDRDPGKRPIMGRIAASMSDLAIVTSDNPRTEDPFSILEQIRGGITPLGIREYGVDELADRSGKGLAEGADKGFVS
LENRREAISLAVRLARPGDIVLLAGKGHEDYQIIGRTKHHFDDREEARLALAEKEG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=493, Percent_Identity=36.9168356997972, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1786274, Length=384, Percent_Identity=27.8645833333333, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 57515; Mature: 57515

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQVRTLGMREPVRPYCTPSGDRMKLTQLLAALDSHDLHGDGAVEIKALTCDSRGIVPDS
CCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCHH
LFFALRGAAMDGHDFIPAAVQAGAVAVVLEDESRAPAAIPWVRVRDGRSAMAWMAAEFHG
HHHHHHCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCC
NPTSRLPLIGITGTNGKTTTTYLIEAILRAAGIPAAVLGTISYRFGDTVITASHTTPEST
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHEECCCEEEEECCCCCCCH
ELQAAFTQLAAAGAKAFVMEVSSHALEQKRVDGCHFDVGIFSNLTRDHLDYHVTMESYRE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHCEEEEEHHHHHH
AKLRLFSELLKPTADKPLRRAAINMDDEQGATVAARAACPVIGYGICRGDVRAEELSLSV
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEHHHCCCCCEEHEECCCCCHHHEEEEE
DGIRARLVTPKGSIDVSSRLLGRFNLSNILAAVSAGIALDLPLEAIRAGIEGHATVPGRM
CCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCHHH
ERVDNRCGITLLVDYAHTGDALENVLTTLKELAQGRIITVFGCGGDRDPGKRPIMGRIAA
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHC
SMSDLAIVTSDNPRTEDPFSILEQIRGGITPLGIREYGVDELADRSGKGLAEGADKGFVS
CCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCEE
LENRREAISLAVRLARPGDIVLLAGKGHEDYQIIGRTKHHFDDREEARLALAEKEG
EHHHHHHEEEEEEEECCCCEEEEECCCCCCEEEECCCHHCCCCHHHHHEEEECCCC
>Mature Secondary Structure
MDQVRTLGMREPVRPYCTPSGDRMKLTQLLAALDSHDLHGDGAVEIKALTCDSRGIVPDS
CCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCHH
LFFALRGAAMDGHDFIPAAVQAGAVAVVLEDESRAPAAIPWVRVRDGRSAMAWMAAEFHG
HHHHHHCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCC
NPTSRLPLIGITGTNGKTTTTYLIEAILRAAGIPAAVLGTISYRFGDTVITASHTTPEST
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHEECCCEEEEECCCCCCCH
ELQAAFTQLAAAGAKAFVMEVSSHALEQKRVDGCHFDVGIFSNLTRDHLDYHVTMESYRE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHCEEEEEHHHHHH
AKLRLFSELLKPTADKPLRRAAINMDDEQGATVAARAACPVIGYGICRGDVRAEELSLSV
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEHHHCCCCCEEHEECCCCCHHHEEEEE
DGIRARLVTPKGSIDVSSRLLGRFNLSNILAAVSAGIALDLPLEAIRAGIEGHATVPGRM
CCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCHHH
ERVDNRCGITLLVDYAHTGDALENVLTTLKELAQGRIITVFGCGGDRDPGKRPIMGRIAA
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHC
SMSDLAIVTSDNPRTEDPFSILEQIRGGITPLGIREYGVDELADRSGKGLAEGADKGFVS
CCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCEE
LENRREAISLAVRLARPGDIVLLAGKGHEDYQIIGRTKHHFDDREEARLALAEKEG
EHHHHHHEEEEEEEECCCCEEEEECCCCCCEEEECCCHHCCCCHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA