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The map label for this gene is murF [H]

Identifier: 118581693

GI number: 118581693

Start: 3629597

End: 3631006

Strand: Reverse

Name: murF [H]

Synonym: Ppro_3293

Alternate gene names: 118581693

Gene position: 3631006-3629597 (Counterclockwise)

Preceding gene: 118581694

Following gene: 118581692

Centisome position: 90.59

GC content: 67.52

Gene sequence:

>1410_bases
ATGTTCAGCATTGCGGAGATAGCCGCTGCCACCGCGGGCGGCTTCCAGGGTGACCGGTCGGCCCGGGTGAGCGGGGTCTC
CACCGATTCGCGCACGGTCGCGGCTGGCCAGCTGTTCGTGCCGCTTAGGGGAGAGCGCTTCGACGGCCACGACTTCATCC
CCCAGGTGGCTGCCAAGGGAGTTGCCGCCGTGCTGGCTGACCAGAGCTTCGACGGGGAGATTCCCCCCGGACTGACCGTT
GTCAGGGTCGTGGATACGCTGGTCGCCCTGGGTGACCTGGCAGCCGCCTACCGCAGGCGCTTTGACCTGCCGCTGGTCGG
TATCACCGGCAGCAACGGCAAGACCACCACCAAGGAGATGCTGGCAACCATCCTGGAGCAGACCGGACCGGGGCTGAAGA
CGGCCGGCAACCTGAACAACCTGATCGGCTTGCCGCAGATGCTGTTCAGGCTTGATGCCTCCCATGGCTGGGCCGTGCTG
GAGATGGGCATGAGCGAGCCGGGCGAGATCGACCGCCTGGCCGAGGTCGCCGCCCCCCAGACCGGTATCGTGCTCAACGC
CTGTCCCGCACACCTGGAGAGCATGGGGAGCGTTGAAGGGGTGGCCAGGGCAAAGGGAGAGCTGTTGCTGCGCCTCCCCG
CCGGAGGGTGCGCCATCATCAATGCCGACGATCCCCTGATCGCCGGACAGCCATCACCCCCAGGGGTGCGGCGACTCACC
TTCGGCCTGGGCGACGCCGACGTGCGCGCCACGGAGATCACCTCGCTGGGAATCGGCGGCCAGAGCTTCCTGCTTCACCT
GGGAGAAATTGTCCTTCCCGTGGTCTTGTGGTCATACGGCAGGCACGCCGTCTATAACGCCCTGGCAGCGGCTGCGGCCG
CCCATGGCTTGGGTATCGCGGCCGAACTGATCCAGAGTGGCCTGGAACGCTTCCGCCCCTACGACAAACGCTTCCAGCTG
GAAGATGTGGGCGGCCTGGTGCTGATCGATGACAGCTACAACGCCAACCCCGCCTCCATGGAGGCGGCCCTGACCACCCT
GGGGGAGCTGAAGGGGGGAGGGCGGGCCTTCGTGGCCCTGGGGGACATGCTGGAGATGGGGAGCAACGAAGCGGAGCTGC
ACCGTGCCCTGGGAGTACGGGCCGCCGCGGTGGCGGACCGGCTCTACCTCTGCGGCCCCTTGATGGCCCACGCGGCGGTG
GGGGCCTTGTCGGCCGGAATGGTTGAGGAGCAGGTCATGCGCATCCCGGACCACGACGAGATCGCTGCCAGCATCCTGCG
TCAGGCGCGTCCGGGAGATTTCGTGCTGGTCAAGGGATCGCGCGGCATGCGCATGGAAAAGGTCGCCGAGGCGATCCGGG
GCCGGACGTTCCTAGGGAGACGGCCCCCTGTGGCTGACCCCACAGCGTAA

Upstream 100 bases:

>100_bases
CGGCAAGGGACATGAGGATTACCAGATCATCGGCAGGACGAAGCATCACTTCGATGATCGCGAAGAGGCCAGGCTGGCGC
TGGCGGAAAAGGAAGGGTAG

Downstream 100 bases:

>100_bases
CGGATTTTTGTAAATCTTGGCGCCACAGCCCCCACGGTGGGCTCGGGCACATACGGAGTAACCCATGCTGTATCACCTGC
TCTATCCATTAGCCGGCGAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MFSIAEIAAATAGGFQGDRSARVSGVSTDSRTVAAGQLFVPLRGERFDGHDFIPQVAAKGVAAVLADQSFDGEIPPGLTV
VRVVDTLVALGDLAAAYRRRFDLPLVGITGSNGKTTTKEMLATILEQTGPGLKTAGNLNNLIGLPQMLFRLDASHGWAVL
EMGMSEPGEIDRLAEVAAPQTGIVLNACPAHLESMGSVEGVARAKGELLLRLPAGGCAIINADDPLIAGQPSPPGVRRLT
FGLGDADVRATEITSLGIGGQSFLLHLGEIVLPVVLWSYGRHAVYNALAAAAAAHGLGIAAELIQSGLERFRPYDKRFQL
EDVGGLVLIDDSYNANPASMEAALTTLGELKGGGRAFVALGDMLEMGSNEAELHRALGVRAAAVADRLYLCGPLMAHAAV
GALSAGMVEEQVMRIPDHDEIAASILRQARPGDFVLVKGSRGMRMEKVAEAIRGRTFLGRRPPVADPTA

Sequences:

>Translated_469_residues
MFSIAEIAAATAGGFQGDRSARVSGVSTDSRTVAAGQLFVPLRGERFDGHDFIPQVAAKGVAAVLADQSFDGEIPPGLTV
VRVVDTLVALGDLAAAYRRRFDLPLVGITGSNGKTTTKEMLATILEQTGPGLKTAGNLNNLIGLPQMLFRLDASHGWAVL
EMGMSEPGEIDRLAEVAAPQTGIVLNACPAHLESMGSVEGVARAKGELLLRLPAGGCAIINADDPLIAGQPSPPGVRRLT
FGLGDADVRATEITSLGIGGQSFLLHLGEIVLPVVLWSYGRHAVYNALAAAAAAHGLGIAAELIQSGLERFRPYDKRFQL
EDVGGLVLIDDSYNANPASMEAALTTLGELKGGGRAFVALGDMLEMGSNEAELHRALGVRAAAVADRLYLCGPLMAHAAV
GALSAGMVEEQVMRIPDHDEIAASILRQARPGDFVLVKGSRGMRMEKVAEAIRGRTFLGRRPPVADPTA
>Mature_469_residues
MFSIAEIAAATAGGFQGDRSARVSGVSTDSRTVAAGQLFVPLRGERFDGHDFIPQVAAKGVAAVLADQSFDGEIPPGLTV
VRVVDTLVALGDLAAAYRRRFDLPLVGITGSNGKTTTKEMLATILEQTGPGLKTAGNLNNLIGLPQMLFRLDASHGWAVL
EMGMSEPGEIDRLAEVAAPQTGIVLNACPAHLESMGSVEGVARAKGELLLRLPAGGCAIINADDPLIAGQPSPPGVRRLT
FGLGDADVRATEITSLGIGGQSFLLHLGEIVLPVVLWSYGRHAVYNALAAAAAAHGLGIAAELIQSGLERFRPYDKRFQL
EDVGGLVLIDDSYNANPASMEAALTTLGELKGGGRAFVALGDMLEMGSNEAELHRALGVRAAAVADRLYLCGPLMAHAAV
GALSAGMVEEQVMRIPDHDEIAASILRQARPGDFVLVKGSRGMRMEKVAEAIRGRTFLGRRPPVADPTA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=441, Percent_Identity=38.0952380952381, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1786279, Length=253, Percent_Identity=30.8300395256917, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49015; Mature: 49015

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSIAEIAAATAGGFQGDRSARVSGVSTDSRTVAAGQLFVPLRGERFDGHDFIPQVAAKG
CCCHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEECEEEEEECCCCCCCCHHHHHHHHHH
VAAVLADQSFDGEIPPGLTVVRVVDTLVALGDLAAAYRRRFDLPLVGITGSNGKTTTKEM
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
LATILEQTGPGLKTAGNLNNLIGLPQMLFRLDASHGWAVLEMGMSEPGEIDRLAEVAAPQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCC
TGIVLNACPAHLESMGSVEGVARAKGELLLRLPAGGCAIINADDPLIAGQPSPPGVRRLT
CCEEEECCHHHHHHHCCHHHHHHCCCCEEEEECCCCEEEECCCCCEECCCCCCCCCEEEE
FGLGDADVRATEITSLGIGGQSFLLHLGEIVLPVVLWSYGRHAVYNALAAAAAAHGLGIA
ECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHH
AELIQSGLERFRPYDKRFQLEDVGGLVLIDDSYNANPASMEAALTTLGELKGGGRAFVAL
HHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
GDMLEMGSNEAELHRALGVRAAAVADRLYLCGPLMAHAAVGALSAGMVEEQVMRIPDHDE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IAASILRQARPGDFVLVKGSRGMRMEKVAEAIRGRTFLGRRPPVADPTA
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFSIAEIAAATAGGFQGDRSARVSGVSTDSRTVAAGQLFVPLRGERFDGHDFIPQVAAKG
CCCHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEECEEEEEECCCCCCCCHHHHHHHHHH
VAAVLADQSFDGEIPPGLTVVRVVDTLVALGDLAAAYRRRFDLPLVGITGSNGKTTTKEM
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
LATILEQTGPGLKTAGNLNNLIGLPQMLFRLDASHGWAVLEMGMSEPGEIDRLAEVAAPQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCC
TGIVLNACPAHLESMGSVEGVARAKGELLLRLPAGGCAIINADDPLIAGQPSPPGVRRLT
CCEEEECCHHHHHHHCCHHHHHHCCCCEEEEECCCCEEEECCCCCEECCCCCCCCCEEEE
FGLGDADVRATEITSLGIGGQSFLLHLGEIVLPVVLWSYGRHAVYNALAAAAAAHGLGIA
ECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHH
AELIQSGLERFRPYDKRFQLEDVGGLVLIDDSYNANPASMEAALTTLGELKGGGRAFVAL
HHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
GDMLEMGSNEAELHRALGVRAAAVADRLYLCGPLMAHAAVGALSAGMVEEQVMRIPDHDE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IAASILRQARPGDFVLVKGSRGMRMEKVAEAIRGRTFLGRRPPVADPTA
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]