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The map label for this gene is miaA

Identifier: 118581646

GI number: 118581646

Start: 3564552

End: 3565493

Strand: Reverse

Name: miaA

Synonym: Ppro_3245

Alternate gene names: 118581646

Gene position: 3565493-3564552 (Counterclockwise)

Preceding gene: 118581647

Following gene: 118581644

Centisome position: 88.96

GC content: 66.67

Gene sequence:

>942_bases
TTGAACGTGTCCAACGCCCCCCGCTTCAACCTGCTGACCATCCTTGGTCCCACCGCCTCGGGCAAGACCCGGCTGGCCGT
TAATCTGGCCCGGGAACTGGGGGGGGAGATCATCTCCGCCGACTCCCGCCAGGTTTTCCGGCGCATGGACATCGGCACCG
GCAAGGACCTGCACGAGTACGGCGAGGTCCACCACCACCTGATCGACATCCTGGAGCCGGGGGAGGAGTTCAGCGTGTTC
GCCTTCCAGCGGCTGTTCCTTGAGGCTGTGGGGGATATTTGCGGTCGGGGGCGCTTGCCGCTCCTCTGCGGCGGCACCGG
CATGTACCTGGACGCGGCGCTCAGGCGTTACCGCATGCATGAGGTCCCCGAGGACCGGGAGTGGCGGGCTTCTCTGGAGG
GGGTCGGCGACGGGGAGTTGGCGTCCCGCCTGCGTGAATTCCGCCCCGGGCTGCACAACAGCACCGACCTCGTGGACCGG
CAACGCACCATCCGCGCCCTGGAGATAGCCCGCTTCCAGGCGGACTGCGCAGGGGATGACGAACCGTTTCCCGATCTGCG
CCCCCTGGTGATCGGCATCCGCTGGGAGCGGGCCGAACTGCGCCGCAGGATTACGGAGCGGCTGCGCCAGCGCCTGGAGA
GCGGAATGATCGAGGAGGTGCGGCGGCTGAACGACGGGGGGGTGCCCTGGGAGCGGCTGGACTACTACGGCCTGGAGTAC
CGCTTCGTGGGGATGTATCTGCGGGATGAACTTAGCCGCAACGACCTGTTCCAGAAGCTGAACAGCGCCATCCATGACTT
CGCCAAGCGCCAGGAGACCTGGTTCCGCAGGATGGAGCGCAACGGGGTGGCGATCAACTGGGTCGACGGTGGGGGAGGTC
CCCTGAGCGAGGCACGGCGGGTGATTCTGGACAACAGCTACCATCTTGCAACCGGCAGGTGA

Upstream 100 bases:

>100_bases
GCCTGGAGATCGCCTTCCCCAGCCGCAGCATCTATATCCGTGGCGGTGAAGATCGGTTTGAACCGGAGACAGGGCAACTC
CCCCGTGAGGCAGAAACGAA

Downstream 100 bases:

>100_bases
GGCTGTCCGTAGCGAGCGACTTATCCATGATCCGTCGTGGTTCCCGGCGAAATGCAGCGGAAGGCAGCCGGACTGTCTGA
GCCCGGAGGGCGAGTTTCCG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 2; DMAPP:tRNA dimethylallyltransferase 2; DMATase 2; Isopentenyl-diphosphate:tRNA isopentenyltransferase 2; IPP transferase 2; IPPT 2; IPTase 2

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNVSNAPRFNLLTILGPTASGKTRLAVNLARELGGEIISADSRQVFRRMDIGTGKDLHEYGEVHHHLIDILEPGEEFSVF
AFQRLFLEAVGDICGRGRLPLLCGGTGMYLDAALRRYRMHEVPEDREWRASLEGVGDGELASRLREFRPGLHNSTDLVDR
QRTIRALEIARFQADCAGDDEPFPDLRPLVIGIRWERAELRRRITERLRQRLESGMIEEVRRLNDGGVPWERLDYYGLEY
RFVGMYLRDELSRNDLFQKLNSAIHDFAKRQETWFRRMERNGVAINWVDGGGGPLSEARRVILDNSYHLATGR

Sequences:

>Translated_313_residues
MNVSNAPRFNLLTILGPTASGKTRLAVNLARELGGEIISADSRQVFRRMDIGTGKDLHEYGEVHHHLIDILEPGEEFSVF
AFQRLFLEAVGDICGRGRLPLLCGGTGMYLDAALRRYRMHEVPEDREWRASLEGVGDGELASRLREFRPGLHNSTDLVDR
QRTIRALEIARFQADCAGDDEPFPDLRPLVIGIRWERAELRRRITERLRQRLESGMIEEVRRLNDGGVPWERLDYYGLEY
RFVGMYLRDELSRNDLFQKLNSAIHDFAKRQETWFRRMERNGVAINWVDGGGGPLSEARRVILDNSYHLATGR
>Mature_313_residues
MNVSNAPRFNLLTILGPTASGKTRLAVNLARELGGEIISADSRQVFRRMDIGTGKDLHEYGEVHHHLIDILEPGEEFSVF
AFQRLFLEAVGDICGRGRLPLLCGGTGMYLDAALRRYRMHEVPEDREWRASLEGVGDGELASRLREFRPGLHNSTDLVDR
QRTIRALEIARFQADCAGDDEPFPDLRPLVIGIRWERAELRRRITERLRQRLESGMIEEVRRLNDGGVPWERLDYYGLEY
RFVGMYLRDELSRNDLFQKLNSAIHDFAKRQETWFRRMERNGVAINWVDGGGGPLSEARRVILDNSYHLATGR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=25.8064516129032, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1790613, Length=303, Percent_Identity=33.003300330033, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25144712, Length=115, Percent_Identity=33.0434782608696, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=23.5294117647059, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24649830, Length=242, Percent_Identity=28.9256198347107, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA2_PELPD (A1AU18)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902896.1
- ProteinModelPortal:   A1AU18
- SMR:   A1AU18
- STRING:   A1AU18
- GeneID:   4572481
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3245
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   IYYGLEY
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35962; Mature: 35962

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSNAPRFNLLTILGPTASGKTRLAVNLARELGGEIISADSRQVFRRMDIGTGKDLHEY
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHCCCCCCCHHHH
GEVHHHLIDILEPGEEFSVFAFQRLFLEAVGDICGRGRLPLLCGGTGMYLDAALRRYRMH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHH
EVPEDREWRASLEGVGDGELASRLREFRPGLHNSTDLVDRQRTIRALEIARFQADCAGDD
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EPFPDLRPLVIGIRWERAELRRRITERLRQRLESGMIEEVRRLNDGGVPWERLDYYGLEY
CCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCC
RFVGMYLRDELSRNDLFQKLNSAIHDFAKRQETWFRRMERNGVAINWVDGGGGPLSEARR
EEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
VILDNSYHLATGR
HHHCCCEEECCCC
>Mature Secondary Structure
MNVSNAPRFNLLTILGPTASGKTRLAVNLARELGGEIISADSRQVFRRMDIGTGKDLHEY
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHCCCCCCCHHHH
GEVHHHLIDILEPGEEFSVFAFQRLFLEAVGDICGRGRLPLLCGGTGMYLDAALRRYRMH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHH
EVPEDREWRASLEGVGDGELASRLREFRPGLHNSTDLVDRQRTIRALEIARFQADCAGDD
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EPFPDLRPLVIGIRWERAELRRRITERLRQRLESGMIEEVRRLNDGGVPWERLDYYGLEY
CCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCC
RFVGMYLRDELSRNDLFQKLNSAIHDFAKRQETWFRRMERNGVAINWVDGGGGPLSEARR
EEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
VILDNSYHLATGR
HHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA