Click here to switch to the map view.

The map label for this gene is mrpB [H]

Identifier: 118581620

GI number: 118581620

Start: 3537615

End: 3538022

Strand: Direct

Name: mrpB [H]

Synonym: Ppro_3219

Alternate gene names: 118581620

Gene position: 3537615-3538022 (Clockwise)

Preceding gene: 118581619

Following gene: 118581621

Centisome position: 88.26

GC content: 65.2

Gene sequence:

>408_bases
ATGTATTCCCTGATCCTTGCCACCGCCATACGGATCCTGCTACCACTGATGCTGCTGTTCTCCCTCTTCCTGCTGCTGCG
GGGGCACAACGAGCCTGGGGGGGGCTTCGCGGGAGGACTGGTGGCGGCGGCCGCCTTCGCGCTCTACAGTCTGGCCCACG
GGGAGCAGGAGGGACGCCGGCTGCTGCGGGTGGATCCCCTCCGGCTGGTCGCAACGGGTCTGCTGATGGCGCTGGCGAGC
GGACTGCTCTCGCTCCCCATGGGGCTTCCCTTTCTCACCGCCGCCTGGAGCAGCGTGCCGGTTCCGGGTATCGGCCACGT
CGGCACACCGCTCCTGTTCGACGCAGGAGTGTATGTACTGGTCGTTGGGATTGCCCTTTTGATTATCTTCACGCTGATGG
AGGAGTGA

Upstream 100 bases:

>100_bases
TCCGTGCCCTGGATACGCTGGGAGAAATCACCGTCCTGGCGGTAGCGGCCCTGGGGGTATACGGATTGCTGAAGAAACGG
TCCGGGGAGGATAGCTAACC

Downstream 100 bases:

>100_bases
ATGGAAAGCGTACTGGCGGTGGTCATTGCCGGACTGTACACCACGGGCATGTACCTGATGGTGCGGCGGAGCATCGTCAA
GATGGTCTTCGGTCTGGCCC

Product: putative monovalent cation/H+ antiporter subunit B

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit B; Multiple resistance and pH homeostasis protein B [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MYSLILATAIRILLPLMLLFSLFLLLRGHNEPGGGFAGGLVAAAAFALYSLAHGEQEGRRLLRVDPLRLVATGLLMALAS
GLLSLPMGLPFLTAAWSSVPVPGIGHVGTPLLFDAGVYVLVVGIALLIIFTLMEE

Sequences:

>Translated_135_residues
MYSLILATAIRILLPLMLLFSLFLLLRGHNEPGGGFAGGLVAAAAFALYSLAHGEQEGRRLLRVDPLRLVATGLLMALAS
GLLSLPMGLPFLTAAWSSVPVPGIGHVGTPLLFDAGVYVLVVGIALLIIFTLMEE
>Mature_135_residues
MYSLILATAIRILLPLMLLFSLFLLLRGHNEPGGGFAGGLVAAAAFALYSLAHGEQEGRRLLRVDPLRLVATGLLMALAS
GLLSLPMGLPFLTAAWSSVPVPGIGHVGTPLLFDAGVYVLVVGIALLIIFTLMEE

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG2111

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhB subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005281
- InterPro:   IPR007182 [H]

Pfam domain/function: PF04039 MnhB [H]

EC number: 1.6.5.3

Molecular weight: Translated: 14193; Mature: 14193

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSLILATAIRILLPLMLLFSLFLLLRGHNEPGGGFAGGLVAAAAFALYSLAHGEQEGRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCH
LLRVDPLRLVATGLLMALASGLLSLPMGLPFLTAAWSSVPVPGIGHVGTPLLFDAGVYVL
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
VVGIALLIIFTLMEE
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MYSLILATAIRILLPLMLLFSLFLLLRGHNEPGGGFAGGLVAAAAFALYSLAHGEQEGRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCH
LLRVDPLRLVATGLLMALASGLLSLPMGLPFLTAAWSSVPVPGIGHVGTPLLFDAGVYVL
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
VVGIALLIIFTLMEE
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]