Click here to switch to the map view.

The map label for this gene is ybjS [C]

Identifier: 118581519

GI number: 118581519

Start: 3420905

End: 3421900

Strand: Direct

Name: ybjS [C]

Synonym: Ppro_3118

Alternate gene names: 118581519

Gene position: 3420905-3421900 (Clockwise)

Preceding gene: 118581518

Following gene: 118581520

Centisome position: 85.35

GC content: 69.08

Gene sequence:

>996_bases
TTGAGGGCACTGGTCACCGGTGGCGGCGGTTTTCTGGGGGGCGCCATCGCGCGCATGCTGCGGGAGCGGGGAGACCAGGT
GCGCAGCTTCTCCCGGGGAGAGTACCCACATCTGGCCCGACTGGGGGTGGAGCAGTGCCGCGGCGATCTGACAGACCGGG
AGGCCCTGCTGGGGGCGGCCCGGGGGTGCGACATCGTCTTCCACGTGGCCGCCAAAGCTGGGGTCTGGGGGAGTTATGAC
GAGTTCCACCGCCCCAACGTCATCGGCACCCAGAACGTGCTTGCCGCCTGCACGAGCCTGGGCATCTCCCGCCTGGTCTT
CACCAGTTCCCCCAGCGTGGTCTTCGACGGACGGGACGTGGAGGGGGGGGACGAGTCACTCCCCTACCCGTCCCGTTACC
ACGCCCATTACCCGCGCACCAAGGCCCTGGCCGAGCGGATGGTGCTGGCCGCCAACAGCCCCGCGTTGGCCACGGTCTCG
TTACGGCCGCACCTGATCTGGGGGCCGGGGGACAACCATCTGGTGCCGCGCATCCTGGCCAGGGCACGGGCGGGCAAACT
CCGCCGCATCGGCTCGTGCCCCTGCCTGGTGGACACGGTCTACGTGGACAACGCGGCCCAGGCCCACCTGCTGGCCGCCG
ACCGTCTTACCCCCGGAGCGGCGCTCTGCGGCAGGGCCTATTTCATCTCCAATGGCGAGCCGATTCCTCTGTGGGAGATG
GTCAACCGTATTCTGGCTGCCGCCGACCTGGCGCCGGTGGAGAGAAGCATCCCTCCCTGGCTGGCCTATGTTGCGGGCTC
CCTGTGCGAATCCCTCTGGGGATGGCTGGGACGGAAAAACGAGCCCCCCATGACCCGCTTCGTGGCCCGGGAGCTGGCCA
CGGCCCACTGGTTCGACATCTCCGCTGCCCGCCGCGATCTGGGGTATCAGCCGGAAGTCACCCTGGAGGAGGGGTTCCGG
CGCCTCCGGGAATGGCTCTCCCGCAGCCGGATCTGA

Upstream 100 bases:

>100_bases
TACTCAAGATCCTCTTCCACCCCTCCTTCCCGGTGGACATCCGCCACAACGCCAAGATCTTCCGCGAAAAGCTGGCAATC
TGGGCCGCGGAGCAGTCCCG

Downstream 100 bases:

>100_bases
CCTTCCCATGGCCACCTCCCCACGCCAGCACTCCCCGCTGTCTCTCCCTCCCCAAGGCGACATCCATCTCCTGCTCGTCC
GCCTCTCCAGCATAACCGGG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRALVTGGGGFLGGAIARMLRERGDQVRSFSRGEYPHLARLGVEQCRGDLTDREALLGAARGCDIVFHVAAKAGVWGSYD
EFHRPNVIGTQNVLAACTSLGISRLVFTSSPSVVFDGRDVEGGDESLPYPSRYHAHYPRTKALAERMVLAANSPALATVS
LRPHLIWGPGDNHLVPRILARARAGKLRRIGSCPCLVDTVYVDNAAQAHLLAADRLTPGAALCGRAYFISNGEPIPLWEM
VNRILAAADLAPVERSIPPWLAYVAGSLCESLWGWLGRKNEPPMTRFVARELATAHWFDISAARRDLGYQPEVTLEEGFR
RLREWLSRSRI

Sequences:

>Translated_331_residues
MRALVTGGGGFLGGAIARMLRERGDQVRSFSRGEYPHLARLGVEQCRGDLTDREALLGAARGCDIVFHVAAKAGVWGSYD
EFHRPNVIGTQNVLAACTSLGISRLVFTSSPSVVFDGRDVEGGDESLPYPSRYHAHYPRTKALAERMVLAANSPALATVS
LRPHLIWGPGDNHLVPRILARARAGKLRRIGSCPCLVDTVYVDNAAQAHLLAADRLTPGAALCGRAYFISNGEPIPLWEM
VNRILAAADLAPVERSIPPWLAYVAGSLCESLWGWLGRKNEPPMTRFVARELATAHWFDISAARRDLGYQPEVTLEEGFR
RLREWLSRSRI
>Mature_331_residues
MRALVTGGGGFLGGAIARMLRERGDQVRSFSRGEYPHLARLGVEQCRGDLTDREALLGAARGCDIVFHVAAKAGVWGSYD
EFHRPNVIGTQNVLAACTSLGISRLVFTSSPSVVFDGRDVEGGDESLPYPSRYHAHYPRTKALAERMVLAANSPALATVS
LRPHLIWGPGDNHLVPRILARARAGKLRRIGSCPCLVDTVYVDNAAQAHLLAADRLTPGAALCGRAYFISNGEPIPLWEM
VNRILAAADLAPVERSIPPWLAYVAGSLCESLWGWLGRKNEPPMTRFVARELATAHWFDISAARRDLGYQPEVTLEEGFR
RLREWLSRSRI

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=325, Percent_Identity=36.6153846153846, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI8393516, Length=325, Percent_Identity=36.6153846153846, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI116268111, Length=330, Percent_Identity=32.7272727272727, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI310132178, Length=330, Percent_Identity=33.6363636363636, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI310113012, Length=330, Percent_Identity=33.6363636363636, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI239745448, Length=330, Percent_Identity=33.6363636363636, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI260763931, Length=352, Percent_Identity=30.3977272727273, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI4504509, Length=352, Percent_Identity=30.3977272727273, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI4504507, Length=353, Percent_Identity=30.028328611898, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI19923621, Length=352, Percent_Identity=32.9545454545455, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI218563686, Length=162, Percent_Identity=38.8888888888889, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI218563684, Length=162, Percent_Identity=38.8888888888889, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI87081792, Length=347, Percent_Identity=30.835734870317, Blast_Score=131, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17570557, Length=329, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193211272, Length=334, Percent_Identity=27.2455089820359, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17509709, Length=283, Percent_Identity=26.5017667844523, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321437, Length=347, Percent_Identity=33.1412103746398, Blast_Score=156, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 36318; Mature: 36318

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALVTGGGGFLGGAIARMLRERGDQVRSFSRGEYPHLARLGVEQCRGDLTDREALLGAA
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
RGCDIVFHVAAKAGVWGSYDEFHRPNVIGTQNVLAACTSLGISRLVFTSSPSVVFDGRDV
CCCCEEEEEHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEECCCCC
EGGDESLPYPSRYHAHYPRTKALAERMVLAANSPALATVSLRPHLIWGPGDNHLVPRILA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCHHHHHHH
RARAGKLRRIGSCPCLVDTVYVDNAAQAHLLAADRLTPGAALCGRAYFISNGEPIPLWEM
HHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHCCEEEECCCCCCCHHHH
VNRILAAADLAPVERSIPPWLAYVAGSLCESLWGWLGRKNEPPMTRFVARELATAHWFDI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SAARRDLGYQPEVTLEEGFRRLREWLSRSRI
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRALVTGGGGFLGGAIARMLRERGDQVRSFSRGEYPHLARLGVEQCRGDLTDREALLGAA
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
RGCDIVFHVAAKAGVWGSYDEFHRPNVIGTQNVLAACTSLGISRLVFTSSPSVVFDGRDV
CCCCEEEEEHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEECCCCC
EGGDESLPYPSRYHAHYPRTKALAERMVLAANSPALATVSLRPHLIWGPGDNHLVPRILA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCHHHHHHH
RARAGKLRRIGSCPCLVDTVYVDNAAQAHLLAADRLTPGAALCGRAYFISNGEPIPLWEM
HHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHCCEEEECCCCCCCHHHH
VNRILAAADLAPVERSIPPWLAYVAGSLCESLWGWLGRKNEPPMTRFVARELATAHWFDI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SAARRDLGYQPEVTLEEGFRRLREWLSRSRI
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]