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The map label for this gene is lcfB [H]

Identifier: 118581518

GI number: 118581518

Start: 3419274

End: 3420908

Strand: Direct

Name: lcfB [H]

Synonym: Ppro_3117

Alternate gene names: 118581518

Gene position: 3419274-3420908 (Clockwise)

Preceding gene: 118581506

Following gene: 118581519

Centisome position: 85.31

GC content: 67.09

Gene sequence:

>1635_bases
ATGAGTTCACATCTCGTCAACATCGCAGCCCATCTCCCCCGCATGGCCGATCTCCAGCCGGACACCCCGGCCATCATCTT
CCCCCGTAACGACATCCAGCTCAGCTTCCGGGAGCTGGACGAGCTCTCCGACCGCATCGCCCACGGCCTGACCGCCATCG
GCATCAGCCGCGGCGTACGCACCGTGCTGATGGTTCCGCCAGGGCCGGAATTCTTCGCCCTCACCTTCGCCCTGTTCAAG
GTGGCAGCGGTACCGGTACTGGTGGATCCCGGCCTGGGCGTCAAGAACCTGAAGACCTGCCTGGACGAGGCCGAGCCCCA
GGCCTTCATCGGCATAACCAAGGCGCACCTGGCCCGACTGCTGTTCGGCTGGGGCAAGGGGAGCATCCATACCCTGGTCA
CCGTGGGCAGACGCCTGCTCTGGCGGGGCGCCACCCTCTCCAGCCTGATCGCCGACAGCGACGAGGAGCCGTTCCCCCCG
GCCAGGACCGGCCGGGGAGAGACCGCAGCCATCCTCTTCACCAGCGGCAGCACCGGCCCCCCCAAGGGAGCGGTGTACAA
CCACGGCAACTTCGCCGCCCAGGTCAGCGCCCTGCGCCAATCCTACGGCATCCGTCCGGGGGAGATCGACCTGCCCACCT
TCCCGCTCTTCGCCCTCTTCGCCCCTGCCCTGGGCATGACCGCCGTGATCCCCGAGATGGACTTCACCCGTCCCGGCTCG
GTCAACCCGGCCATTATCATTTCCACCATCCAGCAGTATGGCGCCACCACCATGTTCGGCTCCCCGGCCCTGATTGATCG
GGTCAGCCGCTACGGCGCCCAACAGGGAATCACGCTCCCGAGCCTGCGGCGGGTCATCTCCGCCGGCGCGCCGGTTCCGG
CGGCGGTCATGGAGCGCTTCAGCGACATGCTCGACCCGGAGACGGAGATATTCACCCCCTACGGCGCAACCGAGGCGCTG
CCGGTCTGCTCCATCGGCAGCAGCGAGGTGCTGGCCGAGACCCGCCATCTGACCGAGCAGGGGCGCGGGGTCTGCGTCGG
CCTGCCGGTTGCGGGGATAGATCTGGCCATCATCCCCATCAGCGACACCCCCATCCGTCACTGGGGCGACGACCTGGCGC
TCCCCGACTGGGAGGTGGGGGAGATCACCGTCAAGGGTCCCCAGGTGACCGCCTCCTACCTGAACCGCCCCGAAGCCACC
AGCCTGGCCAAGATCCCCGATCCCTCGGGCGGCTTCTGGCACCGCATGGGAGACCTAGGCTACCTGGACGGGGAGGGGAG
GGTCTGGTTCTGCGGCCGCAAGGCCCACCGGGTGATCACCCCCATGGATACGCTCTACACCATCCCGGTGGAGGGGGTCT
TCAACAGCCATCCCCAGGTGAAGCGCAGTGCCCTGGTGGGACTGGGCAAACCGGGCAGGCAGCAGCCGGTGCTCTGCGTG
GAAACGGAGACCGGGGTCAGCCAGGAGGAGCATGGCCGCATCAGGCGCGAACTTATCGCCCTGGGGAGCAAATATCCCCA
CACCAAGTCCATACTCAAGATCCTCTTCCACCCCTCCTTCCCGGTGGACATCCGCCACAACGCCAAGATCTTCCGCGAAA
AGCTGGCAATCTGGGCCGCGGAGCAGTCCCGTTGA

Upstream 100 bases:

>100_bases
CTTCGTCCGCCACGACCACAAGCGCTTGCATTGCCCCGGCCGGGTGAGTATATTCAGCCAGATCAACCCTTTCGGAATAC
CCTTTATTCAAAGGCACGCC

Downstream 100 bases:

>100_bases
GGGCACTGGTCACCGGTGGCGGCGGTTTTCTGGGGGGCGCCATCGCGCGCATGCTGCGGGAGCGGGGAGACCAGGTGCGC
AGCTTCTCCCGGGGAGAGTA

Product: peptide synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSSHLVNIAAHLPRMADLQPDTPAIIFPRNDIQLSFRELDELSDRIAHGLTAIGISRGVRTVLMVPPGPEFFALTFALFK
VAAVPVLVDPGLGVKNLKTCLDEAEPQAFIGITKAHLARLLFGWGKGSIHTLVTVGRRLLWRGATLSSLIADSDEEPFPP
ARTGRGETAAILFTSGSTGPPKGAVYNHGNFAAQVSALRQSYGIRPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRPGS
VNPAIIISTIQQYGATTMFGSPALIDRVSRYGAQQGITLPSLRRVISAGAPVPAAVMERFSDMLDPETEIFTPYGATEAL
PVCSIGSSEVLAETRHLTEQGRGVCVGLPVAGIDLAIIPISDTPIRHWGDDLALPDWEVGEITVKGPQVTASYLNRPEAT
SLAKIPDPSGGFWHRMGDLGYLDGEGRVWFCGRKAHRVITPMDTLYTIPVEGVFNSHPQVKRSALVGLGKPGRQQPVLCV
ETETGVSQEEHGRIRRELIALGSKYPHTKSILKILFHPSFPVDIRHNAKIFREKLAIWAAEQSR

Sequences:

>Translated_544_residues
MSSHLVNIAAHLPRMADLQPDTPAIIFPRNDIQLSFRELDELSDRIAHGLTAIGISRGVRTVLMVPPGPEFFALTFALFK
VAAVPVLVDPGLGVKNLKTCLDEAEPQAFIGITKAHLARLLFGWGKGSIHTLVTVGRRLLWRGATLSSLIADSDEEPFPP
ARTGRGETAAILFTSGSTGPPKGAVYNHGNFAAQVSALRQSYGIRPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRPGS
VNPAIIISTIQQYGATTMFGSPALIDRVSRYGAQQGITLPSLRRVISAGAPVPAAVMERFSDMLDPETEIFTPYGATEAL
PVCSIGSSEVLAETRHLTEQGRGVCVGLPVAGIDLAIIPISDTPIRHWGDDLALPDWEVGEITVKGPQVTASYLNRPEAT
SLAKIPDPSGGFWHRMGDLGYLDGEGRVWFCGRKAHRVITPMDTLYTIPVEGVFNSHPQVKRSALVGLGKPGRQQPVLCV
ETETGVSQEEHGRIRRELIALGSKYPHTKSILKILFHPSFPVDIRHNAKIFREKLAIWAAEQSR
>Mature_543_residues
SSHLVNIAAHLPRMADLQPDTPAIIFPRNDIQLSFRELDELSDRIAHGLTAIGISRGVRTVLMVPPGPEFFALTFALFKV
AAVPVLVDPGLGVKNLKTCLDEAEPQAFIGITKAHLARLLFGWGKGSIHTLVTVGRRLLWRGATLSSLIADSDEEPFPPA
RTGRGETAAILFTSGSTGPPKGAVYNHGNFAAQVSALRQSYGIRPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRPGSV
NPAIIISTIQQYGATTMFGSPALIDRVSRYGAQQGITLPSLRRVISAGAPVPAAVMERFSDMLDPETEIFTPYGATEALP
VCSIGSSEVLAETRHLTEQGRGVCVGLPVAGIDLAIIPISDTPIRHWGDDLALPDWEVGEITVKGPQVTASYLNRPEATS
LAKIPDPSGGFWHRMGDLGYLDGEGRVWFCGRKAHRVITPMDTLYTIPVEGVFNSHPQVKRSALVGLGKPGRQQPVLCVE
TETGVSQEEHGRIRRELIALGSKYPHTKSILKILFHPSFPVDIRHNAKIFREKLAIWAAEQSR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=489, Percent_Identity=23.5173824130879, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693145, Length=479, Percent_Identity=24.4258872651357, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI221142682, Length=518, Percent_Identity=25.6756756756757, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786801, Length=479, Percent_Identity=23.3820459290188, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1789201, Length=394, Percent_Identity=22.8426395939086, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=562, Percent_Identity=23.3096085409253, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=562, Percent_Identity=23.3096085409253, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=22.6120857699805, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=562, Percent_Identity=23.3096085409253, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=559, Percent_Identity=23.613595706619, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=331, Percent_Identity=23.2628398791541, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=355, Percent_Identity=23.943661971831, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=466, Percent_Identity=19.9570815450644, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71985884, Length=325, Percent_Identity=23.6923076923077, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI18859661, Length=309, Percent_Identity=26.2135922330097, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 58891; Mature: 58759

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHLVNIAAHLPRMADLQPDTPAIIFPRNDIQLSFRELDELSDRIAHGLTAIGISRGVR
CCCCEEHHHHHCCHHCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCE
TVLMVPPGPEFFALTFALFKVAAVPVLVDPGLGVKNLKTCLDEAEPQAFIGITKAHLARL
EEEEECCCCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHH
LFGWGKGSIHTLVTVGRRLLWRGATLSSLIADSDEEPFPPARTGRGETAAILFTSGSTGP
HHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
PKGAVYNHGNFAAQVSALRQSYGIRPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRPGS
CCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCC
VNPAIIISTIQQYGATTMFGSPALIDRVSRYGAQQGITLPSLRRVISAGAPVPAAVMERF
CCCHHHEEEHHHHCCEEECCCHHHHHHHHHHCHHCCCCHHHHHHHHHCCCCCHHHHHHHH
SDMLDPETEIFTPYGATEALPVCSIGSSEVLAETRHLTEQGRGVCVGLPVAGIDLAIIPI
HHHCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEC
SDTPIRHWGDDLALPDWEVGEITVKGPQVTASYLNRPEATSLAKIPDPSGGFWHRMGDLG
CCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCE
YLDGEGRVWFCGRKAHRVITPMDTLYTIPVEGVFNSHPQVKRSALVGLGKPGRQQPVLCV
EECCCCCEEEECCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEEE
ETETGVSQEEHGRIRRELIALGSKYPHTKSILKILFHPSFPVDIRHNAKIFREKLAIWAA
ECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCC
EQSR
CCCC
>Mature Secondary Structure 
SSHLVNIAAHLPRMADLQPDTPAIIFPRNDIQLSFRELDELSDRIAHGLTAIGISRGVR
CCCEEHHHHHCCHHCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCE
TVLMVPPGPEFFALTFALFKVAAVPVLVDPGLGVKNLKTCLDEAEPQAFIGITKAHLARL
EEEEECCCCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHH
LFGWGKGSIHTLVTVGRRLLWRGATLSSLIADSDEEPFPPARTGRGETAAILFTSGSTGP
HHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
PKGAVYNHGNFAAQVSALRQSYGIRPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRPGS
CCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCC
VNPAIIISTIQQYGATTMFGSPALIDRVSRYGAQQGITLPSLRRVISAGAPVPAAVMERF
CCCHHHEEEHHHHCCEEECCCHHHHHHHHHHCHHCCCCHHHHHHHHHCCCCCHHHHHHHH
SDMLDPETEIFTPYGATEALPVCSIGSSEVLAETRHLTEQGRGVCVGLPVAGIDLAIIPI
HHHCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEC
SDTPIRHWGDDLALPDWEVGEITVKGPQVTASYLNRPEATSLAKIPDPSGGFWHRMGDLG
CCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCE
YLDGEGRVWFCGRKAHRVITPMDTLYTIPVEGVFNSHPQVKRSALVGLGKPGRQQPVLCV
EECCCCCEEEECCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEEE
ETETGVSQEEHGRIRRELIALGSKYPHTKSILKILFHPSFPVDIRHNAKIFREKLAIWAA
ECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCC
EQSR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]