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The map label for this gene is rimO

Identifier: 118581497

GI number: 118581497

Start: 3383275

End: 3384627

Strand: Direct

Name: rimO

Synonym: Ppro_3094

Alternate gene names: 118581497

Gene position: 3383275-3384627 (Clockwise)

Preceding gene: 118581496

Following gene: 118581498

Centisome position: 84.41

GC content: 60.98

Gene sequence:

>1353_bases
GTGGAATCAACAGAGAAACAGAAAGTCAGCATGGTCAGCCTGGGCTGCTCAAAGAACCTGGTGGACGCCGAGGTGATGCT
GGGACTGCTCGCCCGACAGGAGTACGAGATCACCACCGACGAGCGCGAGGCAGACATCATCATCGTCAACACCTGTTCGT
TCATCAAGGAAGCCAAGCAGGAGAGCATCGATGCCATCCTGGATCTGGCCGAGCGCAAGAACGACGGACGCTGCCATACC
CTGATCGTCTCCGGCTGCCTGCCCCAGCGCTATCAGGAGGAGCTGGCGCGGGAACTTCCCGAGGTGGATATCTTCATCGG
CACCGGCGACTACCCGCGCATCGCCGAGATCCTGGCGGAAAAGTCCGGCACGGACGAGCAGCTGTGTTATATCGGCGACC
CGGACTTCGTCTTTGACGAAACTCTGCCGCGGCTCAACTCCTCACCTGCCTGGTTCTCCTATCTCAAGATCGGCGAGGGG
TGCTCCAACCGTTGCAGCTACTGCATCATCCCCAAGCTGCGGGGGCCGTACCGTTCACGACCGCTGGAGGCGCTGGTGGC
CGAGGCGGAGCAGTTAGCCAGCCGGGGTGTCAAGGAGCTGAATATCATCTCCCAGGACATCACCCGCTACGGCAGCGATA
TGGAGGACGGCACCACCCTGGAGACTCTGTTGCGCCGCCTGGTGCAGATCGACGGCATCCAGTGGATACGGCTTTTGTAC
GCCTATCCGGACGGCATCAGCGACGCCCTGATCGCCCTGATCCGCGACGAGCCGAAGATCTGCAAATACCTGGACATCCC
GCTGCAACACATCAGCGACCCGGTCCTGAAGCTCATGCGCCGCCGCAGCAACGAACAGCAGATCAGGGAACTGCTCGCAA
AACTGCGCCGGGAGATTCCGACCCTGGCGCTGCGCACCTCCCTGATCGTCGGCTTTCCCGGCGAAACGATGGAAGACTTC
ACCAGCCTGATGCAGTTCGTGGAACAGGCCCGCTTTGACCGCCTGGGGGTATTCTGCTATTCTCGTGAGGAAGGAACGCC
AGCCGCCACCATGCCGGATCAGGTTTCCGAGCGGGTCAAGCGCGAACGCCACCGCAAACTGATGCGCATCCAGGCCCGTC
TCTCCTTCAAGCGCAACCGCGAACTGATCGGAACAACCGAGCAGGTCATCGTCGAGGGGTACAGCGAAGAGACCGAACTG
CTGCTCAAGGGACGCTCTTCGCGCCAGGCGCCCGACATCGACGGCCAGGTGTATATCACCGCCGGTACCGCCGATGTTGG
GGATATCGTCGCCCTCAGGATAACCGATTCATCGGACTACGACCTGATTGGAGAGATCCAGGAAAGGACCTGA

Upstream 100 bases:

>100_bases
CCGTCATCAGCCCCCCCCGCATTCCCAACAAAAACGATTGCCTTTACAACGCACAATCCGTATAAAAGCACGTCAACAAC
CGTAGAGGCACGAGGTACAC

Downstream 100 bases:

>100_bases
AAAGAGCTTCGACCCAATCGCCTTTGGCTGGCCCATTTATCTTGACAAGCAGTTTTTTTTTTGCTAGAAGTGCCGCCTTC
AAATGCGGCCCCCGGCCACG

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MESTEKQKVSMVSLGCSKNLVDAEVMLGLLARQEYEITTDEREADIIIVNTCSFIKEAKQESIDAILDLAERKNDGRCHT
LIVSGCLPQRYQEELARELPEVDIFIGTGDYPRIAEILAEKSGTDEQLCYIGDPDFVFDETLPRLNSSPAWFSYLKIGEG
CSNRCSYCIIPKLRGPYRSRPLEALVAEAEQLASRGVKELNIISQDITRYGSDMEDGTTLETLLRRLVQIDGIQWIRLLY
AYPDGISDALIALIRDEPKICKYLDIPLQHISDPVLKLMRRRSNEQQIRELLAKLRREIPTLALRTSLIVGFPGETMEDF
TSLMQFVEQARFDRLGVFCYSREEGTPAATMPDQVSERVKRERHRKLMRIQARLSFKRNRELIGTTEQVIVEGYSEETEL
LLKGRSSRQAPDIDGQVYITAGTADVGDIVALRITDSSDYDLIGEIQERT

Sequences:

>Translated_450_residues
MESTEKQKVSMVSLGCSKNLVDAEVMLGLLARQEYEITTDEREADIIIVNTCSFIKEAKQESIDAILDLAERKNDGRCHT
LIVSGCLPQRYQEELARELPEVDIFIGTGDYPRIAEILAEKSGTDEQLCYIGDPDFVFDETLPRLNSSPAWFSYLKIGEG
CSNRCSYCIIPKLRGPYRSRPLEALVAEAEQLASRGVKELNIISQDITRYGSDMEDGTTLETLLRRLVQIDGIQWIRLLY
AYPDGISDALIALIRDEPKICKYLDIPLQHISDPVLKLMRRRSNEQQIRELLAKLRREIPTLALRTSLIVGFPGETMEDF
TSLMQFVEQARFDRLGVFCYSREEGTPAATMPDQVSERVKRERHRKLMRIQARLSFKRNRELIGTTEQVIVEGYSEETEL
LLKGRSSRQAPDIDGQVYITAGTADVGDIVALRITDSSDYDLIGEIQERT
>Mature_450_residues
MESTEKQKVSMVSLGCSKNLVDAEVMLGLLARQEYEITTDEREADIIIVNTCSFIKEAKQESIDAILDLAERKNDGRCHT
LIVSGCLPQRYQEELARELPEVDIFIGTGDYPRIAEILAEKSGTDEQLCYIGDPDFVFDETLPRLNSSPAWFSYLKIGEG
CSNRCSYCIIPKLRGPYRSRPLEALVAEAEQLASRGVKELNIISQDITRYGSDMEDGTTLETLLRRLVQIDGIQWIRLLY
AYPDGISDALIALIRDEPKICKYLDIPLQHISDPVLKLMRRRSNEQQIRELLAKLRREIPTLALRTSLIVGFPGETMEDF
TSLMQFVEQARFDRLGVFCYSREEGTPAATMPDQVSERVKRERHRKLMRIQARLSFKRNRELIGTTEQVIVEGYSEETEL
LLKGRSSRQAPDIDGQVYITAGTADVGDIVALRITDSSDYDLIGEIQERT

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=28.3333333333333, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=28.3333333333333, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=29.3986636971047, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=39.4736842105263, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=32.2869955156951, Blast_Score=224, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=26.4270613107822, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71996771, Length=377, Percent_Identity=30.5039787798408, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21356207, Length=476, Percent_Identity=29.6218487394958, Blast_Score=198, Evalue=6e-51,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=27.8867102396514, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_PELPD (A1ATL9)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902747.1
- ProteinModelPortal:   A1ATL9
- SMR:   A1ATL9
- STRING:   A1ATL9
- GeneID:   4573967
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3094
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   A1ATL9
- ProtClustDB:   CLSK829158
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51088; Mature: 51088

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTEKQKVSMVSLGCSKNLVDAEVMLGLLARQEYEITTDEREADIIIVNTCSFIKEAKQ
CCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHH
ESIDAILDLAERKNDGRCHTLIVSGCLPQRYQEELARELPEVDIFIGTGDYPRIAEILAE
HHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
KSGTDEQLCYIGDPDFVFDETLPRLNSSPAWFSYLKIGEGCSNRCSYCIIPKLRGPYRSR
CCCCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
PLEALVAEAEQLASRGVKELNIISQDITRYGSDMEDGTTLETLLRRLVQIDGIQWIRLLY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
AYPDGISDALIALIRDEPKICKYLDIPLQHISDPVLKLMRRRSNEQQIRELLAKLRREIP
HCCCCHHHHHHHHHCCCCHHHHHHCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCC
TLALRTSLIVGFPGETMEDFTSLMQFVEQARFDRLGVFCYSREEGTPAATMPDQVSERVK
HHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHH
RERHRKLMRIQARLSFKRNRELIGTTEQVIVEGYSEETELLLKGRSSRQAPDIDGQVYIT
HHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEEE
AGTADVGDIVALRITDSSDYDLIGEIQERT
ECCCCCCCEEEEEEECCCCCCHHHHHHCCC
>Mature Secondary Structure
MESTEKQKVSMVSLGCSKNLVDAEVMLGLLARQEYEITTDEREADIIIVNTCSFIKEAKQ
CCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHH
ESIDAILDLAERKNDGRCHTLIVSGCLPQRYQEELARELPEVDIFIGTGDYPRIAEILAE
HHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
KSGTDEQLCYIGDPDFVFDETLPRLNSSPAWFSYLKIGEGCSNRCSYCIIPKLRGPYRSR
CCCCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
PLEALVAEAEQLASRGVKELNIISQDITRYGSDMEDGTTLETLLRRLVQIDGIQWIRLLY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
AYPDGISDALIALIRDEPKICKYLDIPLQHISDPVLKLMRRRSNEQQIRELLAKLRREIP
HCCCCHHHHHHHHHCCCCHHHHHHCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCC
TLALRTSLIVGFPGETMEDFTSLMQFVEQARFDRLGVFCYSREEGTPAATMPDQVSERVK
HHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHH
RERHRKLMRIQARLSFKRNRELIGTTEQVIVEGYSEETELLLKGRSSRQAPDIDGQVYIT
HHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEEE
AGTADVGDIVALRITDSSDYDLIGEIQERT
ECCCCCCCEEEEEEECCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA