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The map label for this gene is hemK [H]

Identifier: 118581410

GI number: 118581410

Start: 3278009

End: 3278875

Strand: Reverse

Name: hemK [H]

Synonym: Ppro_3005

Alternate gene names: 118581410

Gene position: 3278875-3278009 (Counterclockwise)

Preceding gene: 118581413

Following gene: 118581409

Centisome position: 81.81

GC content: 66.9

Gene sequence:

>867_bases
ATGACGGATCAGGAAACCTGGACCACTCTCAGGATTCTCACCTGGACCAAGGAGTACTTTGGCGCCCGGGGAATAGAAAA
TGCCCGGCTGGAGGCCGAGTGGCTGCTCTGCGCCGCCACCGGCCTGGACCGGGTCGGGCTGTACCTCAACTACGACAAGC
CGTTGAACCGCGAGGAACTGTCCGCCTTTCGCCAGATGGTGGCCCGCCGGGCGCGGCGGGAGCCTCTGCAGCACATCCTC
GGCAGTCAGGAGTTCTGCGGCCTGGAGTTCGCGGTTTCGCCGGATGTGCTCATCCCGCGCCACGATACGGAGACCCTGGT
GGAAGAGGCGCTCAGGCGGGCGCCTCTGGCGCGTACGGTTCTGGACATCGGCACCGGCAGCGGCTGCATCGCCGTCAGCC
TGGCAAGGCGCCTGCCAGGTGCCCGGATCGTGGCGAGCGACATCTCTGCCGTGGCGCTGGAAATGGCCCGCGCTAACGCC
CGGGCCAATGGGGTGGATGTGGAATTCCTGCACGGCTCGCTGCTGGAGCCGGTTGCCGGGCGTTGCTTCGACCTGATCGT
CTCCAACCCTCCCTACATTCCCAGCGCGGATATCCAACTGCTGGAGCCGGAGGTGCGGGACGGCGATCCACGGCTGGCCC
TGGACGGCGGGCCGGACGGACTGGACATCTATCGACGCCTGATCCCGGCCAGCCTGGAGCATCTGGAACCGGGCGGCTGG
CTGCTGCTGGAGGTGGGCATGGGGCAGGCGCAGGACGTGGCGGAGATGTTCCCCCTGGCTGACGGGTATGGCCAGGTCGT
TAGCTCCCCCGATCCCGGGGGGATTGAGCGGGTGGTGGGAGCGGCACGAAAGGTGGAATTGACATGA

Upstream 100 bases:

>100_bases
TCTCAAAAATAGCACAGGCGCCCAGGTCGTCAAGGCATTGGAATTGTCTGGAGTGACGCCGGAGAATGGGGTAGTATGCC
ACGCTTCAAGGAGGCGCGGC

Downstream 100 bases:

>100_bases
CGTTTGACGAGGCACGGCGCGTTCTGGACGAGGCAGACCTGCTGATGGGTGAGGAAGAAATCCAGGGGGCCATCGGTCGC
ATGGCGGGCGAGATCACGGG

Product: HemK family modification methylase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTDQETWTTLRILTWTKEYFGARGIENARLEAEWLLCAATGLDRVGLYLNYDKPLNREELSAFRQMVARRARREPLQHIL
GSQEFCGLEFAVSPDVLIPRHDTETLVEEALRRAPLARTVLDIGTGSGCIAVSLARRLPGARIVASDISAVALEMARANA
RANGVDVEFLHGSLLEPVAGRCFDLIVSNPPYIPSADIQLLEPEVRDGDPRLALDGGPDGLDIYRRLIPASLEHLEPGGW
LLLEVGMGQAQDVAEMFPLADGYGQVVSSPDPGGIERVVGAARKVELT

Sequences:

>Translated_288_residues
MTDQETWTTLRILTWTKEYFGARGIENARLEAEWLLCAATGLDRVGLYLNYDKPLNREELSAFRQMVARRARREPLQHIL
GSQEFCGLEFAVSPDVLIPRHDTETLVEEALRRAPLARTVLDIGTGSGCIAVSLARRLPGARIVASDISAVALEMARANA
RANGVDVEFLHGSLLEPVAGRCFDLIVSNPPYIPSADIQLLEPEVRDGDPRLALDGGPDGLDIYRRLIPASLEHLEPGGW
LLLEVGMGQAQDVAEMFPLADGYGQVVSSPDPGGIERVVGAARKVELT
>Mature_287_residues
TDQETWTTLRILTWTKEYFGARGIENARLEAEWLLCAATGLDRVGLYLNYDKPLNREELSAFRQMVARRARREPLQHILG
SQEFCGLEFAVSPDVLIPRHDTETLVEEALRRAPLARTVLDIGTGSGCIAVSLARRLPGARIVASDISAVALEMARANAR
ANGVDVEFLHGSLLEPVAGRCFDLIVSNPPYIPSADIQLLEPEVRDGDPRLALDGGPDGLDIYRRLIPASLEHLEPGGWL
LLEVGMGQAQDVAEMFPLADGYGQVVSSPDPGGIERVVGAARKVELT

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=255, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1787463, Length=268, Percent_Identity=37.3134328358209, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI87082085, Length=275, Percent_Identity=31.6363636363636, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790830, Length=122, Percent_Identity=36.0655737704918, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324266, Length=279, Percent_Identity=27.9569892473118, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24582226, Length=248, Percent_Identity=32.258064516129, Blast_Score=121, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 31461; Mature: 31330

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQETWTTLRILTWTKEYFGARGIENARLEAEWLLCAATGLDRVGLYLNYDKPLNREEL
CCCCCHHEEEEEEEEEHHHHCCCCCCCCCCHHEEEEEECCCCCCCCEEEECCCCCCHHHH
SAFRQMVARRARREPLQHILGSQEFCGLEFAVSPDVLIPRHDTETLVEEALRRAPLARTV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHCCHHHHH
LDIGTGSGCIAVSLARRLPGARIVASDISAVALEMARANARANGVDVEFLHGSLLEPVAG
HHCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHH
RCFDLIVSNPPYIPSADIQLLEPEVRDGDPRLALDGGPDGLDIYRRLIPASLEHLEPGGW
HHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCE
LLLEVGMGQAQDVAEMFPLADGYGQVVSSPDPGGIERVVGAARKVELT
EEEEECCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHCCHHEEECC
>Mature Secondary Structure 
TDQETWTTLRILTWTKEYFGARGIENARLEAEWLLCAATGLDRVGLYLNYDKPLNREEL
CCCCHHEEEEEEEEEHHHHCCCCCCCCCCHHEEEEEECCCCCCCCEEEECCCCCCHHHH
SAFRQMVARRARREPLQHILGSQEFCGLEFAVSPDVLIPRHDTETLVEEALRRAPLARTV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHCCHHHHH
LDIGTGSGCIAVSLARRLPGARIVASDISAVALEMARANARANGVDVEFLHGSLLEPVAG
HHCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHH
RCFDLIVSNPPYIPSADIQLLEPEVRDGDPRLALDGGPDGLDIYRRLIPASLEHLEPGGW
HHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCE
LLLEVGMGQAQDVAEMFPLADGYGQVVSSPDPGGIERVVGAARKVELT
EEEEECCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA