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The map label for this gene is trmB [H]

Identifier: 118581297

GI number: 118581297

Start: 3164576

End: 3165238

Strand: Reverse

Name: trmB [H]

Synonym: Ppro_2892

Alternate gene names: 118581297

Gene position: 3165238-3164576 (Counterclockwise)

Preceding gene: 118581302

Following gene: 118581296

Centisome position: 78.97

GC content: 57.62

Gene sequence:

>663_bases
GTGCTATTTTGCTGCGGTTATTTTGAACGCAAAGGGTACGCCGTGAATCTTATTCCCATAACCTCCCCCCGGTTTCTCAC
ACCGGAATTGCTGGCCGCCATGCCCGACTGGCGGCAGATCTTTGGAAACGACAACCCGCTGGCCCTGGAGATCGGCTGCG
GCATGGGCGACTTCGTGACCCAGGTGGCGCAGCTGCACCCGGACTGGAATTTTGTCGCCATCGATTATTACAACAAGGGG
TGCCTCAAGACCTGCAAACGCATCGACGACCTGGAGTTGAACAATGTGCGGGTGGTTCGGGACGAGGCTCGCGGTTTCAT
GGTGCGCTGCATTCCTCCCGAATCGTTGCAGGCGGTGGTCATCAACTGCCCCGACCCCTGGCCCAAGATGCGCCACCGCA
AACGCAGGCTGGTGAATGGCGAGTTTGTCGCGTTCCTGGCGGATTTCATGCTGCCGGGGGGGGATTTCCACTTTGCCACT
GATTTCGACGACTACGGCGAGGATGTGGCCCGGATGATGCCGGGCATCGCCGGTTTTGAAAACGTCCTTGCCCCCGATTT
CTATCGCCACACGCGGGAGCAATACCCCCTGTCCAAGTATATGAAGAAATTCATGGCCGAGGGAAAACGGATCTATTTCG
TCCATTACCGCAAGCAGGGCTAA

Upstream 100 bases:

>100_bases
ATGTTAATAACAAATCGGGGGGCTTGCTCCGTTGTACGGCTGTGGGGCAGGGGCGATTTTGGCCCGGCAGTGCAAAAAGC
GGTGATTGTCACGGAGAGTT

Downstream 100 bases:

>100_bases
GGCGGGCACGAAAAGGTCGAGCTTCGCTTCCGGCGCGTCTGCGTTGGCAGGGCGGGGAGCGCTTCTTTTCCGCTCTACGG
ATAGTACACTTCCATGTCTG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MLFCCGYFERKGYAVNLIPITSPRFLTPELLAAMPDWRQIFGNDNPLALEIGCGMGDFVTQVAQLHPDWNFVAIDYYNKG
CLKTCKRIDDLELNNVRVVRDEARGFMVRCIPPESLQAVVINCPDPWPKMRHRKRRLVNGEFVAFLADFMLPGGDFHFAT
DFDDYGEDVARMMPGIAGFENVLAPDFYRHTREQYPLSKYMKKFMAEGKRIYFVHYRKQG

Sequences:

>Translated_220_residues
MLFCCGYFERKGYAVNLIPITSPRFLTPELLAAMPDWRQIFGNDNPLALEIGCGMGDFVTQVAQLHPDWNFVAIDYYNKG
CLKTCKRIDDLELNNVRVVRDEARGFMVRCIPPESLQAVVINCPDPWPKMRHRKRRLVNGEFVAFLADFMLPGGDFHFAT
DFDDYGEDVARMMPGIAGFENVLAPDFYRHTREQYPLSKYMKKFMAEGKRIYFVHYRKQG
>Mature_220_residues
MLFCCGYFERKGYAVNLIPITSPRFLTPELLAAMPDWRQIFGNDNPLALEIGCGMGDFVTQVAQLHPDWNFVAIDYYNKG
CLKTCKRIDDLELNNVRVVRDEARGFMVRCIPPESLQAVVINCPDPWPKMRHRKRRLVNGEFVAFLADFMLPGGDFHFAT
DFDDYGEDVARMMPGIAGFENVLAPDFYRHTREQYPLSKYMKKFMAEGKRIYFVHYRKQG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=183, Percent_Identity=32.7868852459016, Blast_Score=103, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 25513; Mature: 25513

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFCCGYFERKGYAVNLIPITSPRFLTPELLAAMPDWRQIFGNDNPLALEIGCGMGDFVT
CEEEEEEECCCCEEEEEEEECCCCCCCHHHHHCCCHHHHHHCCCCCEEEEEECCCHHHHH
QVAQLHPDWNFVAIDYYNKGCLKTCKRIDDLELNNVRVVRDEARGFMVRCIPPESLQAVV
HHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEE
INCPDPWPKMRHRKRRLVNGEFVAFLADFMLPGGDFHFATDFDDYGEDVARMMPGIAGFE
EECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHH
NVLAPDFYRHTREQYPLSKYMKKFMAEGKRIYFVHYRKQG
HHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCC
>Mature Secondary Structure
MLFCCGYFERKGYAVNLIPITSPRFLTPELLAAMPDWRQIFGNDNPLALEIGCGMGDFVT
CEEEEEEECCCCEEEEEEEECCCCCCCHHHHHCCCHHHHHHCCCCCEEEEEECCCHHHHH
QVAQLHPDWNFVAIDYYNKGCLKTCKRIDDLELNNVRVVRDEARGFMVRCIPPESLQAVV
HHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEE
INCPDPWPKMRHRKRRLVNGEFVAFLADFMLPGGDFHFATDFDDYGEDVARMMPGIAGFE
EECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHH
NVLAPDFYRHTREQYPLSKYMKKFMAEGKRIYFVHYRKQG
HHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA