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The map label for this gene is asnB [H]

Identifier: 118581276

GI number: 118581276

Start: 3141144

End: 3143180

Strand: Reverse

Name: asnB [H]

Synonym: Ppro_2871

Alternate gene names: 118581276

Gene position: 3143180-3141144 (Counterclockwise)

Preceding gene: 118581277

Following gene: 118581275

Centisome position: 78.42

GC content: 52.23

Gene sequence:

>2037_bases
ATGTGCGGTATAGCAGGGATTTTCCATTTCGAGGGGGAAACCTCGCCAACACTGGAACAGGTCTCGACGATGATCTCTCC
TCTGGCGCACCGAGGACCGGATGAAGCCGGCGTGTATCTTGACGGTCGCATAGCCCTCGGCAATGTGAGACTCAGCATTA
TCGGCATTGATGGCGGCACGCAACCGATCAGCAACGAAAACGGAACCCTCTGGATAGTCTACAACGGCGAAACATTCAAC
TATATCGAACTGAAAGAGGAGTTGCTGAAGAAGGGGCATCGTTTCACAACGGAAACCGATACGGAAGTACTGCTCCATCT
CTACGAAGAATACGGAACGGAGTGTCTGCAAAAGATTAACGGCCAGTTTTCCTTTGCCATCTGGGACTCCCGTAAAAGGG
AGCTCTTTCTGGCCAGGGACAGGGTTGGCATCCGGCCGCTTTACTACACGTTCTCAGATGGGAGACTTTATTTTGCATCG
GAGATCAAGGCGATTCTTGCGGCAGGCGGCGCACGCGAACTTGACGGGGAAGCACTCTCGCAAGTTTTTATTTTCTGGTC
GACTCTGCCGGGAAGAACCGTGTTCAAGGGTGTCCGGGAACTCCCCCCGGGACACTGTATGCTGGTTAAGAACGGTTCAG
CTACGCCCGAAGCGTACTGGCGCATTCCCCATTATGCCCACAACGACATGCAAAGTCTGTCTTTGGACGATGCAATAGAG
GAACTTCGGGCGCTGCTTACCGATGCGGTTAAAATTCGACTGCGAGCCGATGTTCCGGTTGGAGCATACCTGAGCGGCGG
GTTGGATTCCTCCATCATTGCCATGCTTGTTTCCAGAAATTTCAACACCAGCCTGACAACATTTTCCATGGGCTTTCAGG
AAGATCCCTTTGATGAAACCCCTTTCCAGGATGAACTGATCCGTTTTATCGGTGTTGATCACCGCAGGGTACTGATTGAC
AATGAGCAGATTCTTGGCTGTCTGCAGAAAACCATCTGGCACTGTGAAACGCCGATTCTGCGTACGGCTCCGGTGCCGTT
GTTCATTCTTTCAAGTCTTGTAAACACCGAAGGATTCAAGGTCGTTTTATGCGGAGAAGGAGCGGACGAACTCTTTGGCG
GATACAATATTTTCAAGGAAGCCAAAATACGCAGCTTTTGGGGAAAACAGCCTGAATCGGCGTGGCGTCCGCTGCTCCTG
CAACGACTCTATCCCTATATCTTCAGCAATCCTTCACGCTTCAGACACTACCTGCAAAGCTTCTTTGCCGTCAAAGCGGA
ACAGTCGAAAGACCCCTTTTTCTCGCACATGCTGCGCTGGAAAAGCAGTGAAAAGAATCTGACCATTTTCTCCGATGACT
ACATCGCCGCGCTGGGTGCTTATGACCCCTTTGAAGAACTGGCCAGCCGGTTGCCACACAGTTTTTTTGACCGCGATCTC
CTGTCGCGCGCCCAGGTCCTTGAAATGGAGCTTTTCCTCTCCAACTACCTCCTCTCCAGCCAGGGAGACAGGGTTGCCAT
GGCCCATTCAGTCGAGGCGCGCCACCCCTTTCTGGACGTCAGGTTAATCGATTTTTCCTTGAGGCTCCCCGGAAAGTGGA
AGCTGAGAGGGCTGAACGAAAAGTACATTCTCAAGCGGGCATTTCAGGGACAGATTCCGGAGCGAATAACGAGGCGGGCC
AAACAGCCGTATCGAGCTCCAATACGGGAACTGTTTTCCCCGCACGCGGCACAGAGCAACGAATATGTCGATTCTCTGCT
TTCAACGGCATATCTGAAAAAGAGCGGTTATTTCAATGTGAACAGGGTAAGCAGTCTCTTTGCGCGATACCGTGGCGCCA
GTGAAGGGTTAACGACCGAATTTCAGAATATGGCCCTGATCGGCGTGCTTTCCACCCAGATACTCCATCAGCAGTTTGTC
GAGGGGGCTTCTTTGTTGAAAGCAGGGCTCCTCAGACCGGACAGGATAATCGATGCAACCGGGGGGTACCCCACATATTG
TGAACAACAGGGGGCTGAGCCGGGAAAGGTAAACTGA

Upstream 100 bases:

>100_bases
GGCTTATAGCCTTCCTGATGCAAAAAATGCCATTCGGCCTGTATCGAAGATTGTAGGCAAACTCGCCACGGACACCGCAG
ATGTTCCGAGGAGACAGTAT

Downstream 100 bases:

>100_bases
ATTCAGCTTCTCAGAAAAGCGTGCAAGTAGAATACAGAGGAGAATTCCATGAATAGCGAGGCACGATCACTGGTCCATGA
TTTCCTGGAAAACAGCGCGG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MCGIAGIFHFEGETSPTLEQVSTMISPLAHRGPDEAGVYLDGRIALGNVRLSIIGIDGGTQPISNENGTLWIVYNGETFN
YIELKEELLKKGHRFTTETDTEVLLHLYEEYGTECLQKINGQFSFAIWDSRKRELFLARDRVGIRPLYYTFSDGRLYFAS
EIKAILAAGGARELDGEALSQVFIFWSTLPGRTVFKGVRELPPGHCMLVKNGSATPEAYWRIPHYAHNDMQSLSLDDAIE
ELRALLTDAVKIRLRADVPVGAYLSGGLDSSIIAMLVSRNFNTSLTTFSMGFQEDPFDETPFQDELIRFIGVDHRRVLID
NEQILGCLQKTIWHCETPILRTAPVPLFILSSLVNTEGFKVVLCGEGADELFGGYNIFKEAKIRSFWGKQPESAWRPLLL
QRLYPYIFSNPSRFRHYLQSFFAVKAEQSKDPFFSHMLRWKSSEKNLTIFSDDYIAALGAYDPFEELASRLPHSFFDRDL
LSRAQVLEMELFLSNYLLSSQGDRVAMAHSVEARHPFLDVRLIDFSLRLPGKWKLRGLNEKYILKRAFQGQIPERITRRA
KQPYRAPIRELFSPHAAQSNEYVDSLLSTAYLKKSGYFNVNRVSSLFARYRGASEGLTTEFQNMALIGVLSTQILHQQFV
EGASLLKAGLLRPDRIIDATGGYPTYCEQQGAEPGKVN

Sequences:

>Translated_678_residues
MCGIAGIFHFEGETSPTLEQVSTMISPLAHRGPDEAGVYLDGRIALGNVRLSIIGIDGGTQPISNENGTLWIVYNGETFN
YIELKEELLKKGHRFTTETDTEVLLHLYEEYGTECLQKINGQFSFAIWDSRKRELFLARDRVGIRPLYYTFSDGRLYFAS
EIKAILAAGGARELDGEALSQVFIFWSTLPGRTVFKGVRELPPGHCMLVKNGSATPEAYWRIPHYAHNDMQSLSLDDAIE
ELRALLTDAVKIRLRADVPVGAYLSGGLDSSIIAMLVSRNFNTSLTTFSMGFQEDPFDETPFQDELIRFIGVDHRRVLID
NEQILGCLQKTIWHCETPILRTAPVPLFILSSLVNTEGFKVVLCGEGADELFGGYNIFKEAKIRSFWGKQPESAWRPLLL
QRLYPYIFSNPSRFRHYLQSFFAVKAEQSKDPFFSHMLRWKSSEKNLTIFSDDYIAALGAYDPFEELASRLPHSFFDRDL
LSRAQVLEMELFLSNYLLSSQGDRVAMAHSVEARHPFLDVRLIDFSLRLPGKWKLRGLNEKYILKRAFQGQIPERITRRA
KQPYRAPIRELFSPHAAQSNEYVDSLLSTAYLKKSGYFNVNRVSSLFARYRGASEGLTTEFQNMALIGVLSTQILHQQFV
EGASLLKAGLLRPDRIIDATGGYPTYCEQQGAEPGKVN
>Mature_678_residues
MCGIAGIFHFEGETSPTLEQVSTMISPLAHRGPDEAGVYLDGRIALGNVRLSIIGIDGGTQPISNENGTLWIVYNGETFN
YIELKEELLKKGHRFTTETDTEVLLHLYEEYGTECLQKINGQFSFAIWDSRKRELFLARDRVGIRPLYYTFSDGRLYFAS
EIKAILAAGGARELDGEALSQVFIFWSTLPGRTVFKGVRELPPGHCMLVKNGSATPEAYWRIPHYAHNDMQSLSLDDAIE
ELRALLTDAVKIRLRADVPVGAYLSGGLDSSIIAMLVSRNFNTSLTTFSMGFQEDPFDETPFQDELIRFIGVDHRRVLID
NEQILGCLQKTIWHCETPILRTAPVPLFILSSLVNTEGFKVVLCGEGADELFGGYNIFKEAKIRSFWGKQPESAWRPLLL
QRLYPYIFSNPSRFRHYLQSFFAVKAEQSKDPFFSHMLRWKSSEKNLTIFSDDYIAALGAYDPFEELASRLPHSFFDRDL
LSRAQVLEMELFLSNYLLSSQGDRVAMAHSVEARHPFLDVRLIDFSLRLPGKWKLRGLNEKYILKRAFQGQIPERITRRA
KQPYRAPIRELFSPHAAQSNEYVDSLLSTAYLKKSGYFNVNRVSSLFARYRGASEGLTTEFQNMALIGVLSTQILHQQFV
EGASLLKAGLLRPDRIIDATGGYPTYCEQQGAEPGKVN

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=410, Percent_Identity=29.0243902439024, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI168229252, Length=410, Percent_Identity=29.0243902439024, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI168229250, Length=410, Percent_Identity=29.0243902439024, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI296010848, Length=383, Percent_Identity=29.242819843342, Blast_Score=136, Evalue=7e-32,
Organism=Homo sapiens, GI296010852, Length=312, Percent_Identity=29.1666666666667, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI296010850, Length=312, Percent_Identity=29.1666666666667, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1786889, Length=397, Percent_Identity=30.9823677581864, Blast_Score=193, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI25147557, Length=396, Percent_Identity=29.7979797979798, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71993933, Length=396, Percent_Identity=29.7979797979798, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17560178, Length=395, Percent_Identity=28.3544303797468, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25147560, Length=341, Percent_Identity=26.9794721407625, Blast_Score=115, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321563, Length=419, Percent_Identity=30.0715990453461, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6325403, Length=420, Percent_Identity=29.2857142857143, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553209, Length=403, Percent_Identity=34.2431761786601, Blast_Score=207, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 76768; Mature: 76768

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIFHFEGETSPTLEQVSTMISPLAHRGPDEAGVYLDGRIALGNVRLSIIGIDGGT
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECEEEEEEEEEEEEEECCCC
QPISNENGTLWIVYNGETFNYIELKEELLKKGHRFTTETDTEVLLHLYEEYGTECLQKIN
CCCCCCCCEEEEEECCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GQFSFAIWDSRKRELFLARDRVGIRPLYYTFSDGRLYFASEIKAILAAGGARELDGEALS
CCEEEEEECCCCCEEEEECCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCCCCHHHHH
QVFIFWSTLPGRTVFKGVRELPPGHCMLVKNGSATPEAYWRIPHYAHNDMQSLSLDDAIE
HHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHEEECCCHHCCCHHHCCHHHHHH
ELRALLTDAVKIRLRADVPVGAYLSGGLDSSIIAMLVSRNFNTSLTTFSMGFQEDPFDET
HHHHHHHHHHEEEEEECCCCCHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
PFQDELIRFIGVDHRRVLIDNEQILGCLQKTIWHCETPILRTAPVPLFILSSLVNTEGFK
CCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHCCCCCE
VVLCGEGADELFGGYNIFKEAKIRSFWGKQPESAWRPLLLQRLYPYIFSNPSRFRHYLQS
EEEECCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FFAVKAEQSKDPFFSHMLRWKSSEKNLTIFSDDYIAALGAYDPFEELASRLPHSFFDRDL
HHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCHHHHHHHCCHHHHHHHH
LSRAQVLEMELFLSNYLLSSQGDRVAMAHSVEARHPFLDVRLIDFSLRLPGKWKLRGLNE
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEEEEECCCCEEECCCCH
KYILKRAFQGQIPERITRRAKQPYRAPIRELFSPHAAQSNEYVDSLLSTAYLKKSGYFNV
HHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
NRVSSLFARYRGASEGLTTEFQNMALIGVLSTQILHQQFVEGASLLKAGLLRPDRIIDAT
HHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
GGYPTYCEQQGAEPGKVN
CCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MCGIAGIFHFEGETSPTLEQVSTMISPLAHRGPDEAGVYLDGRIALGNVRLSIIGIDGGT
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECEEEEEEEEEEEEEECCCC
QPISNENGTLWIVYNGETFNYIELKEELLKKGHRFTTETDTEVLLHLYEEYGTECLQKIN
CCCCCCCCEEEEEECCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GQFSFAIWDSRKRELFLARDRVGIRPLYYTFSDGRLYFASEIKAILAAGGARELDGEALS
CCEEEEEECCCCCEEEEECCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCCCCHHHHH
QVFIFWSTLPGRTVFKGVRELPPGHCMLVKNGSATPEAYWRIPHYAHNDMQSLSLDDAIE
HHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHEEECCCHHCCCHHHCCHHHHHH
ELRALLTDAVKIRLRADVPVGAYLSGGLDSSIIAMLVSRNFNTSLTTFSMGFQEDPFDET
HHHHHHHHHHEEEEEECCCCCHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
PFQDELIRFIGVDHRRVLIDNEQILGCLQKTIWHCETPILRTAPVPLFILSSLVNTEGFK
CCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHCCCCCE
VVLCGEGADELFGGYNIFKEAKIRSFWGKQPESAWRPLLLQRLYPYIFSNPSRFRHYLQS
EEEECCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FFAVKAEQSKDPFFSHMLRWKSSEKNLTIFSDDYIAALGAYDPFEELASRLPHSFFDRDL
HHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCHHHHHHHCCHHHHHHHH
LSRAQVLEMELFLSNYLLSSQGDRVAMAHSVEARHPFLDVRLIDFSLRLPGKWKLRGLNE
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEEEEECCCCEEECCCCH
KYILKRAFQGQIPERITRRAKQPYRAPIRELFSPHAAQSNEYVDSLLSTAYLKKSGYFNV
HHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
NRVSSLFARYRGASEGLTTEFQNMALIGVLSTQILHQQFVEGASLLKAGLLRPDRIIDAT
HHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
GGYPTYCEQQGAEPGKVN
CCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]