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The map label for this gene is ppsA [H]

Identifier: 118581251

GI number: 118581251

Start: 3111225

End: 3113642

Strand: Reverse

Name: ppsA [H]

Synonym: Ppro_2845

Alternate gene names: 118581251

Gene position: 3113642-3111225 (Counterclockwise)

Preceding gene: 118581257

Following gene: 118581249

Centisome position: 77.69

GC content: 64.81

Gene sequence:

>2418_bases
ATGGTAGATCAACAGGACTACATCCGCTGGTTCGAGGAATTATCCATCGATGACGTGGCTCTTGTGGGGGGCAAGAACGC
CTCCCTGGGGGAGATGTACCGCCAGCTCGCCCCCCGCGGGGTCAGGATTCCCAATGGCTTTGCCGTGACCGCCGAGGCCT
ACCGGCGGCTGGTGGCCTCGGCGGGCATCCTCCCCGAGATGGAGCGCCTCCTGGGCGGCATGGACAGGAACGACCTAGCT
GATTTCGCACGGCGGGGGGAGCGGCTGCGGGAACTGGTCTGCCGGGCGCCCCTGCCGGAAGAGGTGGCAACGGAGATCAT
CGCCGCCTATCGGCGTCTGTGCGACCAGTACGGGGAGGAAACGGATGTGGCGGTGCGCAGCAGTGCCACCGCGGAGGACC
TGCCCACCGCCTCCTTTGCCGGTCAGCAGGAAACCTACCTCAATATCCGGGGCAGTGAGCAGCTCCTGGAGGCCTGCCGC
CGCTGTTTCGCCTCCCTGTTCACCGACCGGGCCATCTCCTACCGCCTGGACCAGGGGTTCGATCACTTCCAGGTGGCCCT
CTCCATCGGCGTGATGAAGATGGTCCGCTCCGACCTGGCGGCCAGCGGGGTCATGTTCACCATCGATACGGAAACCGGCT
TCCGGGATGTGGTGCTGATCAACGGTGCCTACGGCCTGGGGGAGAACCTGGTTCAGGGGGCGGTCAACCCGGACGAGTTC
TACGTTTTCAAGCCGACCCTGCGCACCGGTTATCGTCCCATCGTCGGCAGGAAACTGGGTGAGAAGCGACTCAGGATGGT
CTACGGCGGCGACAGGCCGGGTGAACTCACCCGCAATGTGGAGGTGGCGGAGGATGAGCGCGACCGCTTCTGCATCAGCG
ACGCGGAGGTCCTGGAGCTGGCCCGCTATGGCATGATCATCGAGGAGTATTACGCCGACAGGCTTGGCAGGGACAGCCCC
ATGGACATCGAGTGGGCCAAGGATGGGGAGACGGGCCAGCTCTTCATCGTCCAGGCCAGGCCGGAGACGGTCCAGTCCCA
GAAGGACATGGACTGCCTGGAGATTTTTCATCTGGCAGGGAAGGGCGCCCTGCTGGCCTCGGGCACCAGCGTGGGCGAGA
AGATCGCATCGGGCGTCGCCCGGGTGATCACCGACCTGCGCGGCCTGTCCGACTTCAGGCCGGGTGAGATCCTGGTGGCC
GACACCACCACGCCTGACTGGGAACCGGTCATGAAGAAGGCGGCGGCGGTGGTCACGAACCGGGGCGGTCGCACCTGTCA
TGCCGCCATTGTCAGCCGTGAACTGGGCATCCCCTCCATCGTGGGGACCGGCAATGCCACGCAGCGACTCAACAGCGGCC
GGCAGATCACGGTGAGCTGCGCCGAGGGGGAGGTGGGCAGGATCTACGAGGGGCTGATCCCCTTCCGGGTGGAGCGGATC
TCCCTGGCCCACTTGGAGCGGCCGCGCACCAGGATCATGATGAACCTGGGTAACCCGGATGAGGCCTTCGCCCTGGCCAG
GATTCCCAATGACGGCGTCGGGCTGGCCCGCATGGAGTTTATCATCACCACCTACATCAAGATCCACCCCATGGCGCTGG
TCCATCCCGAGCGGATCGCGGGCGAGGAGGAGCGCCGGGAGATCGAGCGTCTCACCGCCGCGTTCAGCCCGCGGGAAGAG
TATTTCGTGGAGCAACTGGCCGGCGGGATCGCCACCATCGCCGCTCCCTTCTACCCCAGGCCGGTGGTGGTGCGGCTCTC
CGATTTCAAAAGCAACGAGTACGCCAGCCTGATCGGCGGCAGGGCTTTCGAGCCGGAGGAGGAGAACCCCATGATCGGCT
TCCGCGGCGCCTCGCGCTATTACGACGAGCGCTATCGGGAGGGGTTCGCCCTGGAGTGCCAGGCCCTGAAGCGGGTCCGC
GACCAGATGGGGCTGGTCAACGTGATTCCCATGATCCCCTTCTGTCGTACGGTGGGGGAGGGGGAGAAGGTTCTTGCCGA
GATGGAGAAGAACGGGCTCACGCGGGGGGAGAACGGTCTCATGGTGTACGTCATGTGCGAAATCCCCAACAACGTCATCC
TGATCGACGAATTCAGCAGGCTCTTCGACGGTTTCTCCATCGGCTCCAACGACCTGACCCAGCTCACCCTGGGGGTGGAC
CGGGATTCGGCCCTGGTGGCCCATGTCTTCGATGAGCGGGACCCGGGGGTGATGCGCTCAGTGGCCAAGGTGATCGAGGG
TGCCCGGCGCAATCACCGCCACAGCGGTATCTGCGGCCAGGCTCCCAGCGACTATCCCGAGTTCGCCGCCTTCCTGGTCA
GGGAAGGGATCGACTCCATCTCTCTCAACCCGGACTCGGTCATGAAGATCACCCTCAGGGTGCTGGAGATGGAGAAGGAA
CTGGAACGGACGAACTGA

Upstream 100 bases:

>100_bases
CGGGTACACTCTTTTACAGGTGTGATGCAGGGACAAGAGGTGTGAGGCGCCAGCGGCGCGGGCCCGCGCCGGTCAGGGGT
ATACCCCGGGGAGAAAGGAC

Downstream 100 bases:

>100_bases
ATGGAAATGCCGGGCTGCCATAGGCGGAGTCGGGGAGCGTCTTTTCGTGCTCCTGCTCCGCCCGTGGCATGCCGGCGCCG
CTAGAGATGGAAGTCGCCGG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 805; Mature: 805

Protein sequence:

>805_residues
MVDQQDYIRWFEELSIDDVALVGGKNASLGEMYRQLAPRGVRIPNGFAVTAEAYRRLVASAGILPEMERLLGGMDRNDLA
DFARRGERLRELVCRAPLPEEVATEIIAAYRRLCDQYGEETDVAVRSSATAEDLPTASFAGQQETYLNIRGSEQLLEACR
RCFASLFTDRAISYRLDQGFDHFQVALSIGVMKMVRSDLAASGVMFTIDTETGFRDVVLINGAYGLGENLVQGAVNPDEF
YVFKPTLRTGYRPIVGRKLGEKRLRMVYGGDRPGELTRNVEVAEDERDRFCISDAEVLELARYGMIIEEYYADRLGRDSP
MDIEWAKDGETGQLFIVQARPETVQSQKDMDCLEIFHLAGKGALLASGTSVGEKIASGVARVITDLRGLSDFRPGEILVA
DTTTPDWEPVMKKAAAVVTNRGGRTCHAAIVSRELGIPSIVGTGNATQRLNSGRQITVSCAEGEVGRIYEGLIPFRVERI
SLAHLERPRTRIMMNLGNPDEAFALARIPNDGVGLARMEFIITTYIKIHPMALVHPERIAGEEERREIERLTAAFSPREE
YFVEQLAGGIATIAAPFYPRPVVVRLSDFKSNEYASLIGGRAFEPEEENPMIGFRGASRYYDERYREGFALECQALKRVR
DQMGLVNVIPMIPFCRTVGEGEKVLAEMEKNGLTRGENGLMVYVMCEIPNNVILIDEFSRLFDGFSIGSNDLTQLTLGVD
RDSALVAHVFDERDPGVMRSVAKVIEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSISLNPDSVMKITLRVLEMEKE
LERTN

Sequences:

>Translated_805_residues
MVDQQDYIRWFEELSIDDVALVGGKNASLGEMYRQLAPRGVRIPNGFAVTAEAYRRLVASAGILPEMERLLGGMDRNDLA
DFARRGERLRELVCRAPLPEEVATEIIAAYRRLCDQYGEETDVAVRSSATAEDLPTASFAGQQETYLNIRGSEQLLEACR
RCFASLFTDRAISYRLDQGFDHFQVALSIGVMKMVRSDLAASGVMFTIDTETGFRDVVLINGAYGLGENLVQGAVNPDEF
YVFKPTLRTGYRPIVGRKLGEKRLRMVYGGDRPGELTRNVEVAEDERDRFCISDAEVLELARYGMIIEEYYADRLGRDSP
MDIEWAKDGETGQLFIVQARPETVQSQKDMDCLEIFHLAGKGALLASGTSVGEKIASGVARVITDLRGLSDFRPGEILVA
DTTTPDWEPVMKKAAAVVTNRGGRTCHAAIVSRELGIPSIVGTGNATQRLNSGRQITVSCAEGEVGRIYEGLIPFRVERI
SLAHLERPRTRIMMNLGNPDEAFALARIPNDGVGLARMEFIITTYIKIHPMALVHPERIAGEEERREIERLTAAFSPREE
YFVEQLAGGIATIAAPFYPRPVVVRLSDFKSNEYASLIGGRAFEPEEENPMIGFRGASRYYDERYREGFALECQALKRVR
DQMGLVNVIPMIPFCRTVGEGEKVLAEMEKNGLTRGENGLMVYVMCEIPNNVILIDEFSRLFDGFSIGSNDLTQLTLGVD
RDSALVAHVFDERDPGVMRSVAKVIEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSISLNPDSVMKITLRVLEMEKE
LERTN
>Mature_805_residues
MVDQQDYIRWFEELSIDDVALVGGKNASLGEMYRQLAPRGVRIPNGFAVTAEAYRRLVASAGILPEMERLLGGMDRNDLA
DFARRGERLRELVCRAPLPEEVATEIIAAYRRLCDQYGEETDVAVRSSATAEDLPTASFAGQQETYLNIRGSEQLLEACR
RCFASLFTDRAISYRLDQGFDHFQVALSIGVMKMVRSDLAASGVMFTIDTETGFRDVVLINGAYGLGENLVQGAVNPDEF
YVFKPTLRTGYRPIVGRKLGEKRLRMVYGGDRPGELTRNVEVAEDERDRFCISDAEVLELARYGMIIEEYYADRLGRDSP
MDIEWAKDGETGQLFIVQARPETVQSQKDMDCLEIFHLAGKGALLASGTSVGEKIASGVARVITDLRGLSDFRPGEILVA
DTTTPDWEPVMKKAAAVVTNRGGRTCHAAIVSRELGIPSIVGTGNATQRLNSGRQITVSCAEGEVGRIYEGLIPFRVERI
SLAHLERPRTRIMMNLGNPDEAFALARIPNDGVGLARMEFIITTYIKIHPMALVHPERIAGEEERREIERLTAAFSPREE
YFVEQLAGGIATIAAPFYPRPVVVRLSDFKSNEYASLIGGRAFEPEEENPMIGFRGASRYYDERYREGFALECQALKRVR
DQMGLVNVIPMIPFCRTVGEGEKVLAEMEKNGLTRGENGLMVYVMCEIPNNVILIDEFSRLFDGFSIGSNDLTQLTLGVD
RDSALVAHVFDERDPGVMRSVAKVIEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSISLNPDSVMKITLRVLEMEKE
LERTN

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=793, Percent_Identity=51.5762925598991, Blast_Score=831, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=431, Percent_Identity=26.2180974477958, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1789193, Length=424, Percent_Identity=27.3584905660377, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994992, Length=392, Percent_Identity=26.530612244898, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788726, Length=390, Percent_Identity=24.8717948717949, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17564524, Length=234, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 89600; Mature: 89600

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDQQDYIRWFEELSIDDVALVGGKNASLGEMYRQLAPRGVRIPNGFAVTAEAYRRLVAS
CCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHH
AGILPEMERLLGGMDRNDLADFARRGERLRELVCRAPLPEEVATEIIAAYRRLCDQYGEE
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
TDVAVRSSATAEDLPTASFAGQQETYLNIRGSEQLLEACRRCFASLFTDRAISYRLDQGF
CCEEEECCCCHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DHFQVALSIGVMKMVRSDLAASGVMFTIDTETGFRDVVLINGAYGLGENLVQGAVNPDEF
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCCHHHHHCCCCCCCE
YVFKPTLRTGYRPIVGRKLGEKRLRMVYGGDRPGELTRNVEVAEDERDRFCISDAEVLEL
EEECCHHHCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEECCHHHHHHH
ARYGMIIEEYYADRLGRDSPMDIEWAKDGETGQLFIVQARPETVQSQKDMDCLEIFHLAG
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHCC
KGALLASGTSVGEKIASGVARVITDLRGLSDFRPGEILVADTTTPDWEPVMKKAAAVVTN
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCC
RGGRTCHAAIVSRELGIPSIVGTGNATQRLNSGRQITVSCAEGEVGRIYEGLIPFRVERI
CCCCEEHHHHHHHHCCCCCEECCCCHHHHHCCCCEEEEEECCCCHHHHHHCCCHHHHHHH
SLAHLERPRTRIMMNLGNPDEAFALARIPNDGVGLARMEFIITTYIKIHPMALVHPERIA
HHHHHCCCCEEEEEECCCCCHHEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECHHHHC
GEEERREIERLTAAFSPREEYFVEQLAGGIATIAAPFYPRPVVVRLSDFKSNEYASLIGG
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHCC
RAFEPEEENPMIGFRGASRYYDERYREGFALECQALKRVRDQMGLVNVIPMIPFCRTVGE
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHCCC
GEKVLAEMEKNGLTRGENGLMVYVMCEIPNNVILIDEFSRLFDGFSIGSNDLTQLTLGVD
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEECHHHHHCCCCCCCCCCEEEEEECC
RDSALVAHVFDERDPGVMRSVAKVIEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSI
CCCHHHEEHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
SLNPDSVMKITLRVLEMEKELERTN
CCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVDQQDYIRWFEELSIDDVALVGGKNASLGEMYRQLAPRGVRIPNGFAVTAEAYRRLVAS
CCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHH
AGILPEMERLLGGMDRNDLADFARRGERLRELVCRAPLPEEVATEIIAAYRRLCDQYGEE
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
TDVAVRSSATAEDLPTASFAGQQETYLNIRGSEQLLEACRRCFASLFTDRAISYRLDQGF
CCEEEECCCCHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DHFQVALSIGVMKMVRSDLAASGVMFTIDTETGFRDVVLINGAYGLGENLVQGAVNPDEF
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCCHHHHHCCCCCCCE
YVFKPTLRTGYRPIVGRKLGEKRLRMVYGGDRPGELTRNVEVAEDERDRFCISDAEVLEL
EEECCHHHCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEECCHHHHHHH
ARYGMIIEEYYADRLGRDSPMDIEWAKDGETGQLFIVQARPETVQSQKDMDCLEIFHLAG
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHCC
KGALLASGTSVGEKIASGVARVITDLRGLSDFRPGEILVADTTTPDWEPVMKKAAAVVTN
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCC
RGGRTCHAAIVSRELGIPSIVGTGNATQRLNSGRQITVSCAEGEVGRIYEGLIPFRVERI
CCCCEEHHHHHHHHCCCCCEECCCCHHHHHCCCCEEEEEECCCCHHHHHHCCCHHHHHHH
SLAHLERPRTRIMMNLGNPDEAFALARIPNDGVGLARMEFIITTYIKIHPMALVHPERIA
HHHHHCCCCEEEEEECCCCCHHEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECHHHHC
GEEERREIERLTAAFSPREEYFVEQLAGGIATIAAPFYPRPVVVRLSDFKSNEYASLIGG
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHCC
RAFEPEEENPMIGFRGASRYYDERYREGFALECQALKRVRDQMGLVNVIPMIPFCRTVGE
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHCCC
GEKVLAEMEKNGLTRGENGLMVYVMCEIPNNVILIDEFSRLFDGFSIGSNDLTQLTLGVD
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEECHHHHHCCCCCCCCCCEEEEEECC
RDSALVAHVFDERDPGVMRSVAKVIEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSI
CCCHHHEEHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
SLNPDSVMKITLRVLEMEKELERTN
CCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]