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The map label for this gene is thiC1 [H]

Identifier: 118581216

GI number: 118581216

Start: 3074612

End: 3075919

Strand: Direct

Name: thiC1 [H]

Synonym: Ppro_2809

Alternate gene names: 118581216

Gene position: 3074612-3075919 (Clockwise)

Preceding gene: 118581215

Following gene: 118581217

Centisome position: 76.71

GC content: 63.76

Gene sequence:

>1308_bases
ATGCTTACCCAGATCGAATCCGCCCGCCAGGGCATCCTCACCCCCCAGATGGAGGCCGTCGCGGCAGCCGAAGAACTCAC
CCCCGCCTACATCCTGCAGATGGTTGCCGAAGGCAAGATCGTCATCCCCTGGAACCACAACAGGAAGCCTAAGGTGTGCG
GCATCGGCAAAGGGTTGCGCACAAAAGTCAACGCCTCCATCGGCACCTCATCGGACATCGTCGACTATGCCGCCGAGGTC
AGGAAGGCCAAGGTGGCCCAGGAAGCGGGCGCCGATACACTGATGGAACTCTCCGTGGGGGGTGACCTTGACCGCGTACG
GCGCGAAGTCATCGCCGCCGTCGACCTGCCGGTGGGCAACGTGCCGCTGTACCAGGCCTTCTGCGAGGCGGCCCGCAAGT
ACGGCAACCCCAACAAGTTGGACGAGGAGATGCTCTTCGACCTGATCGAGCAGCAGTGCGCCGACGGCATGGCATTCATG
GCCGTTCACTGCGGCATCAACCTGTACACCATCGAGCGCCTGCGCAAGCAGGGCTACCGCTACGGCGGCCTGGTCAGCAA
AGGGGGGGTCAGCATGGTGGCCTGGATGGTTGCCAACAACCGTGAAAACCCGCTCTACGAAAAGTTCGACCGTGTCTGTT
CCATCCTCAAGAAGTACGATACCGTCCTCTCCCTGGGCAATGGCCTGCGCGCCGGCGCCATTCATGACTCTTCCGACCGC
GCCCAGATCCAGGAACTGCTGATCAACTGCGAACTGGCCGAACTGGGCCGCGACATGGGGTGCCAGATGCTGGTGGAAGG
CCCGGGACACGTGCCTCTGGACGAGATCGAGGGGAACATCCAGCTGCAGAAGCGCATGAGCGGCGACGCCCCCTACTACA
TGCTGGGGCCCATCGCCACCGACGTGGCCCCCGGCTACGACCACATCACCGCCGCCATCGGCGCGGCCCAGTCCAGCCGC
TTCGGCGCCGACCTGATCTGCTACATCACCCCGGCCGAGCACCTGGCACTGCCCAATGAGGAGGACGTGCGCATGGGGGT
CAAGGCCGCCAAGGTTGCCGCCTACATCGGCGACATGAACAAGTACCCGGAGAAGGGACGCCAGCGTGACAAGGAAATCT
CTAAGGCCCGCCGCGACCTGAACTGGGACAAGCAGTACGAACTGGCCCTGTTCCCGGAAGATGCCAAGGCGATCCGCGAC
AGCCGCACCCCCGAGGACGAGAACACCTGCACCATGTGCGGCGACTTCTGCGCCTCCCGCGGGGCGGGCAGGTTGTTCGC
CGCTGACCTGAAGGGTGACAAGATTTAA

Upstream 100 bases:

>100_bases
CCGATGTTATCCGTTTACATCCGCTCCTTCCGCGTTCCATTTGATTGATCTAGCCACACCACTGACACTTCACATCACCA
TCACCAAGGAGAAAAAACGT

Downstream 100 bases:

>100_bases
CGTTTTTTGCTGCGCCAACGAAGAACGGCGCGCGGAATTTTCCGTGCGCCGTTCTTCGTTGGCGTGTCCTCTGTCGGACT
TCACGAGAATGAAGCGCGGT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 1; HMP-P synthase 1; HMP-phosphate synthase 1; HMPP synthase 1; Thiamine biosynthesis protein thiC 1 [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLTQIESARQGILTPQMEAVAAAEELTPAYILQMVAEGKIVIPWNHNRKPKVCGIGKGLRTKVNASIGTSSDIVDYAAEV
RKAKVAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGNVPLYQAFCEAARKYGNPNKLDEEMLFDLIEQQCADGMAFM
AVHCGINLYTIERLRKQGYRYGGLVSKGGVSMVAWMVANNRENPLYEKFDRVCSILKKYDTVLSLGNGLRAGAIHDSSDR
AQIQELLINCELAELGRDMGCQMLVEGPGHVPLDEIEGNIQLQKRMSGDAPYYMLGPIATDVAPGYDHITAAIGAAQSSR
FGADLICYITPAEHLALPNEEDVRMGVKAAKVAAYIGDMNKYPEKGRQRDKEISKARRDLNWDKQYELALFPEDAKAIRD
SRTPEDENTCTMCGDFCASRGAGRLFAADLKGDKI

Sequences:

>Translated_435_residues
MLTQIESARQGILTPQMEAVAAAEELTPAYILQMVAEGKIVIPWNHNRKPKVCGIGKGLRTKVNASIGTSSDIVDYAAEV
RKAKVAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGNVPLYQAFCEAARKYGNPNKLDEEMLFDLIEQQCADGMAFM
AVHCGINLYTIERLRKQGYRYGGLVSKGGVSMVAWMVANNRENPLYEKFDRVCSILKKYDTVLSLGNGLRAGAIHDSSDR
AQIQELLINCELAELGRDMGCQMLVEGPGHVPLDEIEGNIQLQKRMSGDAPYYMLGPIATDVAPGYDHITAAIGAAQSSR
FGADLICYITPAEHLALPNEEDVRMGVKAAKVAAYIGDMNKYPEKGRQRDKEISKARRDLNWDKQYELALFPEDAKAIRD
SRTPEDENTCTMCGDFCASRGAGRLFAADLKGDKI
>Mature_435_residues
MLTQIESARQGILTPQMEAVAAAEELTPAYILQMVAEGKIVIPWNHNRKPKVCGIGKGLRTKVNASIGTSSDIVDYAAEV
RKAKVAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGNVPLYQAFCEAARKYGNPNKLDEEMLFDLIEQQCADGMAFM
AVHCGINLYTIERLRKQGYRYGGLVSKGGVSMVAWMVANNRENPLYEKFDRVCSILKKYDTVLSLGNGLRAGAIHDSSDR
AQIQELLINCELAELGRDMGCQMLVEGPGHVPLDEIEGNIQLQKRMSGDAPYYMLGPIATDVAPGYDHITAAIGAAQSSR
FGADLICYITPAEHLALPNEEDVRMGVKAAKVAAYIGDMNKYPEKGRQRDKEISKARRDLNWDKQYELALFPEDAKAIRD
SRTPEDENTCTMCGDFCASRGAGRLFAADLKGDKI

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=454, Percent_Identity=36.5638766519824, Blast_Score=300, Evalue=9e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47701; Mature: 47701

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQIESARQGILTPQMEAVAAAEELTPAYILQMVAEGKIVIPWNHNRKPKVCGIGKGLR
CCCCHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCEEECCCCCC
TKVNASIGTSSDIVDYAAEVRKAKVAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGN
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCC
VPLYQAFCEAARKYGNPNKLDEEMLFDLIEQQCADGMAFMAVHCGINLYTIERLRKQGYR
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHCCCC
YGGLVSKGGVSMVAWMVANNRENPLYEKFDRVCSILKKYDTVLSLGNGLRAGAIHDSSDR
CCCEECCCCHHEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHH
AQIQELLINCELAELGRDMGCQMLVEGPGHVPLDEIEGNIQLQKRMSGDAPYYMLGPIAT
HHHHHHHHCCCHHHHCCCCCCEEEECCCCCCCHHHCCCCEEEEECCCCCCCEEEECCHHH
DVAPGYDHITAAIGAAQSSRFGADLICYITPAEHLALPNEEDVRMGVKAAKVAAYIGDMN
HCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KYPEKGRQRDKEISKARRDLNWDKQYELALFPEDAKAIRDSRTPEDENTCTMCGDFCASR
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHCC
GAGRLFAADLKGDKI
CCCCEEEECCCCCCC
>Mature Secondary Structure
MLTQIESARQGILTPQMEAVAAAEELTPAYILQMVAEGKIVIPWNHNRKPKVCGIGKGLR
CCCCHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCEEECCCCCC
TKVNASIGTSSDIVDYAAEVRKAKVAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGN
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCC
VPLYQAFCEAARKYGNPNKLDEEMLFDLIEQQCADGMAFMAVHCGINLYTIERLRKQGYR
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHCCCC
YGGLVSKGGVSMVAWMVANNRENPLYEKFDRVCSILKKYDTVLSLGNGLRAGAIHDSSDR
CCCEECCCCHHEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHH
AQIQELLINCELAELGRDMGCQMLVEGPGHVPLDEIEGNIQLQKRMSGDAPYYMLGPIAT
HHHHHHHHCCCHHHHCCCCCCEEEECCCCCCCHHHCCCCEEEEECCCCCCCEEEECCHHH
DVAPGYDHITAAIGAAQSSRFGADLICYITPAEHLALPNEEDVRMGVKAAKVAAYIGDMN
HCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KYPEKGRQRDKEISKARRDLNWDKQYELALFPEDAKAIRDSRTPEDENTCTMCGDFCASR
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHCC
GAGRLFAADLKGDKI
CCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA