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The map label for this gene is hycE [H]

Identifier: 118581193

GI number: 118581193

Start: 3046752

End: 3048425

Strand: Reverse

Name: hycE [H]

Synonym: Ppro_2786

Alternate gene names: 118581193

Gene position: 3048425-3046752 (Counterclockwise)

Preceding gene: 118581194

Following gene: 118581192

Centisome position: 76.06

GC content: 61.29

Gene sequence:

>1674_bases
ATGGTAAGTAAAAAGCTCCAGCAACTGAAAGACAAATTCGGCTCCGCCATCCAGCAGGCCGACGTAGTCAACGACATGCG
TCTGTTGGTCTACATCGACCCGGCCAGCGTCAAACCGATCTGCCAGTACATCTTCCGCGACATGGATGCCCGCTACGTCA
TCAGCATAGGCTCTGACGACCGTCCGTACAGCGGCAAATTCCTTGTCTTCCACCAGTTCGCCTTTGACAAGGAAAAGATC
CTGTTCAGCATCATCTGCCAGCTTCCGGGTGAGAACCCGCGCATCGACAGCATCTCCGGGATCATTCCCGCTGCCAACTG
GGCCGAGCGCGAACTCAAGGACCTGTTGGGCATCGAGCCTGTGGGTCACCCCTATCCGAAGCGGCTGGTTCTTCCCGACG
CCTGGCCCGATGGTCACCATCCCTTACGGAAAGACTTTGCCTGGAACAAGATTCCGGAAGGGTACGACGAAGAGCGCGAG
TTCGACTTCGACGAGACCCCTCCCGGGTGCACCGCCGTCCCCTTCGGCCCGTTCCATCCGACGCTTGACGAGCCCGAGCA
CTTCCGCCTCTACGTGGATGGCGAATTCGTGCGTGGCTGCGAATACCGCGGCTTCATGGTCCACCGGGGCATCGAGAAGC
TGTCCGAATCGGTACTTGGCTACAACGACGTGCCCATGATGGCCGAGCGCATCTGCGGCATCTGCGGCTGCGTGCACAGC
CTGGCTTTCTGTCAGGCCGTAGAGAACGGGGCCGGGGTCATCGTTCCTCCGCGGGCCGAATTCATCCGCACCATCATGCT
TGAGATCGAGCGTCTGCACAGCCATCCCATGTGGGTCGGCCTTGCCTGCCACCTGGTGGGCTTCGACACCCTGTTCATGC
AGGCCTGGCGCATCCGTGAACCGATCATGTGGTTGGCCGAGAAGATCACCGGCAACCGCAAGACCTACGGCCTTGGCATC
ATCGGTGGCGTTCGCTGGGACTTCACGCCTGAGCTGATCGCCGAGACCCGCGCCGTCCTTGACAAGCTGGAGAGCGAATG
GCGTCAGGTCGTCGCCGCCGTCAGCAAGGACAAGAACATCATGGCCCGTACCAGCGGCGTCGGCATGGCCGACAAGGCGC
TGTGCAAAGAGATGGGCCTGGTAGGTCCGGTCGCCCGTGGGGGCGGCGTTGACATCGACGCCCGTCGTGATCACCCCTAC
GCCGCCTACGACCGGGTGGACTTCAAAGTCATCACCCACAACACCGCCGACGTCTGGGGCCGTGTGGTGGTGCGCATGCT
GGAAGTCTTCGAATCGATCAACATCATCCGCCAGTGCTTTGACAAGATGCCGCAGGGCCCGCTGGTAGCGGACATCACCA
CGGAGCTTCCTGCTGGCCGGGTTGGCTACACCTCCATCGAAGCCCCGCGCGGTGAGAGCCACCACTTCGCCATCACCGGT
GACGACAACCGTCCTCGTCGCTGGCGTGCCCGAGCTCCGACCTATCAGAACCTGCAGGGTATCCCGGCCATGATCAAGGA
CCAGCGCATAGCCGACATGCAGATCTCCTTAGGTGGCATCGACCCGTGCATGTCCTGCACCGACCGCATGGAAACCATCG
ACATCAGGACCGGCGCACGCAAGAGCTGGACAAAAGCCGAACTTCTCTCGTTGTCCAGATCACGGAAAGGCTAG

Upstream 100 bases:

>100_bases
ATCGTTATGCCGCCTCCAACTTCTACGGTGAAATCAAGCGCTGCTTCGGGTGGCTCGGCGGCAATCCGCGTCCCCAGACA
CGCATCAAGGAAGACTAACC

Downstream 100 bases:

>100_bases
GAGGTAGTTGTGGAGAGTAACGTCATGAACATGATAGTGGGCGTCCTGGTAAACGTCATCCTGGTGTTAGCGCTCGCTCC
GCTCTTTCAGGGCGTACTTC

Product: NADH dehydrogenase (ubiquinone), 30 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MVSKKLQQLKDKFGSAIQQADVVNDMRLLVYIDPASVKPICQYIFRDMDARYVISIGSDDRPYSGKFLVFHQFAFDKEKI
LFSIICQLPGENPRIDSISGIIPAANWAERELKDLLGIEPVGHPYPKRLVLPDAWPDGHHPLRKDFAWNKIPEGYDEERE
FDFDETPPGCTAVPFGPFHPTLDEPEHFRLYVDGEFVRGCEYRGFMVHRGIEKLSESVLGYNDVPMMAERICGICGCVHS
LAFCQAVENGAGVIVPPRAEFIRTIMLEIERLHSHPMWVGLACHLVGFDTLFMQAWRIREPIMWLAEKITGNRKTYGLGI
IGGVRWDFTPELIAETRAVLDKLESEWRQVVAAVSKDKNIMARTSGVGMADKALCKEMGLVGPVARGGGVDIDARRDHPY
AAYDRVDFKVITHNTADVWGRVVVRMLEVFESINIIRQCFDKMPQGPLVADITTELPAGRVGYTSIEAPRGESHHFAITG
DDNRPRRWRARAPTYQNLQGIPAMIKDQRIADMQISLGGIDPCMSCTDRMETIDIRTGARKSWTKAELLSLSRSRKG

Sequences:

>Translated_557_residues
MVSKKLQQLKDKFGSAIQQADVVNDMRLLVYIDPASVKPICQYIFRDMDARYVISIGSDDRPYSGKFLVFHQFAFDKEKI
LFSIICQLPGENPRIDSISGIIPAANWAERELKDLLGIEPVGHPYPKRLVLPDAWPDGHHPLRKDFAWNKIPEGYDEERE
FDFDETPPGCTAVPFGPFHPTLDEPEHFRLYVDGEFVRGCEYRGFMVHRGIEKLSESVLGYNDVPMMAERICGICGCVHS
LAFCQAVENGAGVIVPPRAEFIRTIMLEIERLHSHPMWVGLACHLVGFDTLFMQAWRIREPIMWLAEKITGNRKTYGLGI
IGGVRWDFTPELIAETRAVLDKLESEWRQVVAAVSKDKNIMARTSGVGMADKALCKEMGLVGPVARGGGVDIDARRDHPY
AAYDRVDFKVITHNTADVWGRVVVRMLEVFESINIIRQCFDKMPQGPLVADITTELPAGRVGYTSIEAPRGESHHFAITG
DDNRPRRWRARAPTYQNLQGIPAMIKDQRIADMQISLGGIDPCMSCTDRMETIDIRTGARKSWTKAELLSLSRSRKG
>Mature_557_residues
MVSKKLQQLKDKFGSAIQQADVVNDMRLLVYIDPASVKPICQYIFRDMDARYVISIGSDDRPYSGKFLVFHQFAFDKEKI
LFSIICQLPGENPRIDSISGIIPAANWAERELKDLLGIEPVGHPYPKRLVLPDAWPDGHHPLRKDFAWNKIPEGYDEERE
FDFDETPPGCTAVPFGPFHPTLDEPEHFRLYVDGEFVRGCEYRGFMVHRGIEKLSESVLGYNDVPMMAERICGICGCVHS
LAFCQAVENGAGVIVPPRAEFIRTIMLEIERLHSHPMWVGLACHLVGFDTLFMQAWRIREPIMWLAEKITGNRKTYGLGI
IGGVRWDFTPELIAETRAVLDKLESEWRQVVAAVSKDKNIMARTSGVGMADKALCKEMGLVGPVARGGGVDIDARRDHPY
AAYDRVDFKVITHNTADVWGRVVVRMLEVFESINIIRQCFDKMPQGPLVADITTELPAGRVGYTSIEAPRGESHHFAITG
DDNRPRRWRARAPTYQNLQGIPAMIKDQRIADMQISLGGIDPCMSCTDRMETIDIRTGARKSWTKAELLSLSRSRKG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=397, Percent_Identity=27.7078085642317, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI260898743, Length=386, Percent_Identity=27.720207253886, Blast_Score=144, Evalue=3e-34,
Organism=Escherichia coli, GI1789076, Length=557, Percent_Identity=38.4201077199282, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1788832, Length=557, Percent_Identity=36.983842010772, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI145693162, Length=511, Percent_Identity=27.9843444227006, Blast_Score=196, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17555284, Length=381, Percent_Identity=26.7716535433071, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17568379, Length=381, Percent_Identity=27.0341207349081, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI221459469, Length=383, Percent_Identity=29.242819843342, Blast_Score=163, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24638644, Length=381, Percent_Identity=28.0839895013123, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 62847; Mature: 62847

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKKLQQLKDKFGSAIQQADVVNDMRLLVYIDPASVKPICQYIFRDMDARYVISIGSDD
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCC
RPYSGKFLVFHQFAFDKEKILFSIICQLPGENPRIDSISGIIPAANWAERELKDLLGIEP
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
VGHPYPKRLVLPDAWPDGHHPLRKDFAWNKIPEGYDEEREFDFDETPPGCTAVPFGPFHP
CCCCCCCEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TLDEPEHFRLYVDGEFVRGCEYRGFMVHRGIEKLSESVLGYNDVPMMAERICGICGCVHS
CCCCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LAFCQAVENGAGVIVPPRAEFIRTIMLEIERLHSHPMWVGLACHLVGFDTLFMQAWRIRE
HHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
PIMWLAEKITGNRKTYGLGIIGGVRWDFTPELIAETRAVLDKLESEWRQVVAAVSKDKNI
HHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MARTSGVGMADKALCKEMGLVGPVARGGGVDIDARRDHPYAAYDRVDFKVITHNTADVWG
EEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCHHHHH
RVVVRMLEVFESINIIRQCFDKMPQGPLVADITTELPAGRVGYTSIEAPRGESHHFAITG
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEC
DDNRPRRWRARAPTYQNLQGIPAMIKDQRIADMQISLGGIDPCMSCTDRMETIDIRTGAR
CCCCCCCEECCCCCHHCCCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHEEEEECCCCC
KSWTKAELLSLSRSRKG
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSKKLQQLKDKFGSAIQQADVVNDMRLLVYIDPASVKPICQYIFRDMDARYVISIGSDD
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCC
RPYSGKFLVFHQFAFDKEKILFSIICQLPGENPRIDSISGIIPAANWAERELKDLLGIEP
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
VGHPYPKRLVLPDAWPDGHHPLRKDFAWNKIPEGYDEEREFDFDETPPGCTAVPFGPFHP
CCCCCCCEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TLDEPEHFRLYVDGEFVRGCEYRGFMVHRGIEKLSESVLGYNDVPMMAERICGICGCVHS
CCCCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LAFCQAVENGAGVIVPPRAEFIRTIMLEIERLHSHPMWVGLACHLVGFDTLFMQAWRIRE
HHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
PIMWLAEKITGNRKTYGLGIIGGVRWDFTPELIAETRAVLDKLESEWRQVVAAVSKDKNI
HHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MARTSGVGMADKALCKEMGLVGPVARGGGVDIDARRDHPYAAYDRVDFKVITHNTADVWG
EEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCHHHHH
RVVVRMLEVFESINIIRQCFDKMPQGPLVADITTELPAGRVGYTSIEAPRGESHHFAITG
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEC
DDNRPRRWRARAPTYQNLQGIPAMIKDQRIADMQISLGGIDPCMSCTDRMETIDIRTGAR
CCCCCCCEECCCCCHHCCCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHEEEEECCCCC
KSWTKAELLSLSRSRKG
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]