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The map label for this gene is hupR1 [H]

Identifier: 118581130

GI number: 118581130

Start: 2971368

End: 2971934

Strand: Reverse

Name: hupR1 [H]

Synonym: Ppro_2721

Alternate gene names: 118581130

Gene position: 2971934-2971368 (Counterclockwise)

Preceding gene: 118581131

Following gene: 118581129

Centisome position: 74.15

GC content: 55.38

Gene sequence:

>567_bases
ATGACAACCAGGGTCCTGCTGGTCGATGACGAGAAAAATGTGTTGTCCGCACTCAGGCGCGCACTGTTTGACGACCCCTA
TGAGATCATGACTGCTTCCAGCGCCGAAGAGGCTTTGGAGATCATGAAGCTGCATTCTTTCAAGGTCGTTATTTCCGACG
AACGAATGACCGGCATGCAGGGGGTGGATTTTCTCGCCAGGGTTCGGGTCCTTTCCCCGGAGACGGTTCGCATTCTGCTT
ACCGGACATGGGACCATGGAAGCCGCCATGAAAGCGGTCAACCTGGGTGGAATCTACCGTTTTTTCACTAAACCGTGGAA
TGACCTCGAGATCAGGTTCGCGGTCCGCTCAGCCGTCGAGAAGTATGACCTGGAAGAGGAAAACAGGCGCTTGTTGAGCA
CGATCCGGAGTCAGGCCCTGGAATTCAAGGTGCTGGAGCGGCGTTATCCCGGTATCTCCCGTGTGGAGAAGGATCGTCAT
GGCAGCTTCGTGCTGCCGGATATTTCCGAGGAAGACGTGGAGCGCCTGATTGCGGAGTGCGACCTGGAAACAGGCGCAAA
AGAATGA

Upstream 100 bases:

>100_bases
CCAGGTGACCAGTGACTGATCGCCGTGGGGCGGCTGACGAAGAAGCCCGCGGCAGGTGATGCATTGGTGCGCACCGTCGC
ACCAATCGAGGAGATCTCCC

Downstream 100 bases:

>100_bases
CTCCGGAAAGAACGTGCCGGGTTGATGGGGACCATCCATGATGAACAGCCGGAGCGTTGAAGCAGAGACGGGCAGGATCC
TGGTCATCGATGATGACATC

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MTTRVLLVDDEKNVLSALRRALFDDPYEIMTASSAEEALEIMKLHSFKVVISDERMTGMQGVDFLARVRVLSPETVRILL
TGHGTMEAAMKAVNLGGIYRFFTKPWNDLEIRFAVRSAVEKYDLEEENRRLLSTIRSQALEFKVLERRYPGISRVEKDRH
GSFVLPDISEEDVERLIAECDLETGAKE

Sequences:

>Translated_188_residues
MTTRVLLVDDEKNVLSALRRALFDDPYEIMTASSAEEALEIMKLHSFKVVISDERMTGMQGVDFLARVRVLSPETVRILL
TGHGTMEAAMKAVNLGGIYRFFTKPWNDLEIRFAVRSAVEKYDLEEENRRLLSTIRSQALEFKVLERRYPGISRVEKDRH
GSFVLPDISEEDVERLIAECDLETGAKE
>Mature_187_residues
TTRVLLVDDEKNVLSALRRALFDDPYEIMTASSAEEALEIMKLHSFKVVISDERMTGMQGVDFLARVRVLSPETVRILLT
GHGTMEAAMKAVNLGGIYRFFTKPWNDLEIRFAVRSAVEKYDLEEENRRLLSTIRSQALEFKVLERRYPGISRVEKDRHG
SFVLPDISEEDVERLIAECDLETGAKE

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=143, Percent_Identity=30.0699300699301, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 21511; Mature: 21380

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRVLLVDDEKNVLSALRRALFDDPYEIMTASSAEEALEIMKLHSFKVVISDERMTGMQ
CCEEEEEEECCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCH
GVDFLARVRVLSPETVRILLTGHGTMEAAMKAVNLGGIYRFFTKPWNDLEIRFAVRSAVE
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCEEHHHHHHHHH
KYDLEEENRRLLSTIRSQALEFKVLERRYPGISRVEKDRHGSFVLPDISEEDVERLIAEC
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHC
DLETGAKE
CCCCCCCC
>Mature Secondary Structure 
TTRVLLVDDEKNVLSALRRALFDDPYEIMTASSAEEALEIMKLHSFKVVISDERMTGMQ
CEEEEEEECCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCH
GVDFLARVRVLSPETVRILLTGHGTMEAAMKAVNLGGIYRFFTKPWNDLEIRFAVRSAVE
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCEEHHHHHHHHH
KYDLEEENRRLLSTIRSQALEFKVLERRYPGISRVEKDRHGSFVLPDISEEDVERLIAEC
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHC
DLETGAKE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]