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The map label for this gene is rpfG [H]

Identifier: 118581131

GI number: 118581131

Start: 2972016

End: 2973350

Strand: Reverse

Name: rpfG [H]

Synonym: Ppro_2722

Alternate gene names: 118581131

Gene position: 2973350-2972016 (Counterclockwise)

Preceding gene: 118581132

Following gene: 118581130

Centisome position: 74.19

GC content: 57.53

Gene sequence:

>1335_bases
ATGGCTATGGATATGGATGAAACGCATCAGGCCGGGCAGGGGCCGATTTCGGTCCTGCTGGTCGATGACGAAGAGTACAT
ACTGACGTCGCTTCGGCGTCTTTTGATGGATGAGAAGGATATGGAAGTCGTTACCGCCGGTTCAGGGGAGGAAGGGCTGA
ATCTTCTGCCTACACTCGCCAACCTGGGAGTAATTGTATCGGATCAACGGATGCCGGGAATGAACGGCGCCCAGTTTTTG
GAAAAGGCGCGGCTTGTCAGGCCGGATGCGGTGCGGATCATTCTGACCGGGTATGCCGATGTGTCGGCTGCTGTTGACGC
CATCAACAGAGGGGGAGCCTGGCGTTACATGACCAAGCCGTGGAATAACGAGGAGCTAGCGCAAGCCATCCATGACGCCG
TTGAGCTGTATACCCATGAATCCCAGAATCGTCGACTGAATGAAGTCATCAGGCAGCAGAACAGCGAGATGGAGGTGTGG
ACCGAAAACCTCAAGAAACGTCTGCTGCAGAGTACCTCGACCATTCGTGAGCAGAGCCAAGCGCTGAAAGAGCTCAGCCT
GAAGAAGCCGGTGGCAGTTGTTCTTCAGGCATTCGACGGTTTTTTTGAGATCATGGGGGGCTCAAACGCGGTTCATGCCC
GCACCGTAAGCACCCTGGTTGCCGATGTTGCCCGCAAGATGGGGCTCGATGCCGAGACCATAACCCGGTTCCGGTTGGCC
GCGTTGCTCCATGATACCGGCAAGTTCGGAACCCTGGTCGCTGAATTGAACAAAAGCATGGAGGACATGTCCGAAAGCGA
GGCACGGGAATACCGTCGGCATCCGCTTCGCGGCGAAGAGTTGGTCTCCAGGGTCGAAGAGCTCGCGGAGGTCGCTCTGC
TGGTACGCAGCCACCATGAAACCTTCGATGGCCAGGGATTTCCCGATGGACTGGAGGGCGAGCGGATTCCCCTGGGAGCG
CGACTGATCGCCATTGCCGATCTCATCGAGAGGAGCGCGCGATCGGTGGAAAAACACCGCGCCGATTTTGCGTTGATGAC
AGCCCGCTTCCACGGCGGGACCCTGCTCGACCCCCATCTGGTGACGAAGTTTCAGGGAATCACCAGGACCATCTATTTCG
AGGGAAGGAAATCCGGGCTGGCTTCCGAACTGGAGCTGGAGCCCAAGGATCTCATTCCGGGGATGCAGATCAGCCGTGAC
GTAGAGAGTGGGGCGGGGGTTCTGCTCATGCAGCGTGGTACAGTGCTGGATATGGCCGGTATTCTGCTCATTCGCAGTCA
CTTCCACAAGACCCCCTCCCCCCATGGCATTTATGTCCAGGTGACCAGTGACTGA

Upstream 100 bases:

>100_bases
GGCGCCTGCCGAGCGTTCCTGACGCGGATTGAACAGGGGGATCAGCGGGCGATCGTAATTGCGCTGATGAAGGATACTAT
GACGTCCGGAGGAGCCGGAT

Downstream 100 bases:

>100_bases
TCGCCGTGGGGCGGCTGACGAAGAAGCCCGCGGCAGGTGATGCATTGGTGCGCACCGTCGCACCAATCGAGGAGATCTCC
CATGACAACCAGGGTCCTGC

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAMDMDETHQAGQGPISVLLVDDEEYILTSLRRLLMDEKDMEVVTAGSGEEGLNLLPTLANLGVIVSDQRMPGMNGAQFL
EKARLVRPDAVRIILTGYADVSAAVDAINRGGAWRYMTKPWNNEELAQAIHDAVELYTHESQNRRLNEVIRQQNSEMEVW
TENLKKRLLQSTSTIREQSQALKELSLKKPVAVVLQAFDGFFEIMGGSNAVHARTVSTLVADVARKMGLDAETITRFRLA
ALLHDTGKFGTLVAELNKSMEDMSESEAREYRRHPLRGEELVSRVEELAEVALLVRSHHETFDGQGFPDGLEGERIPLGA
RLIAIADLIERSARSVEKHRADFALMTARFHGGTLLDPHLVTKFQGITRTIYFEGRKSGLASELELEPKDLIPGMQISRD
VESGAGVLLMQRGTVLDMAGILLIRSHFHKTPSPHGIYVQVTSD

Sequences:

>Translated_444_residues
MAMDMDETHQAGQGPISVLLVDDEEYILTSLRRLLMDEKDMEVVTAGSGEEGLNLLPTLANLGVIVSDQRMPGMNGAQFL
EKARLVRPDAVRIILTGYADVSAAVDAINRGGAWRYMTKPWNNEELAQAIHDAVELYTHESQNRRLNEVIRQQNSEMEVW
TENLKKRLLQSTSTIREQSQALKELSLKKPVAVVLQAFDGFFEIMGGSNAVHARTVSTLVADVARKMGLDAETITRFRLA
ALLHDTGKFGTLVAELNKSMEDMSESEAREYRRHPLRGEELVSRVEELAEVALLVRSHHETFDGQGFPDGLEGERIPLGA
RLIAIADLIERSARSVEKHRADFALMTARFHGGTLLDPHLVTKFQGITRTIYFEGRKSGLASELELEPKDLIPGMQISRD
VESGAGVLLMQRGTVLDMAGILLIRSHFHKTPSPHGIYVQVTSD
>Mature_443_residues
AMDMDETHQAGQGPISVLLVDDEEYILTSLRRLLMDEKDMEVVTAGSGEEGLNLLPTLANLGVIVSDQRMPGMNGAQFLE
KARLVRPDAVRIILTGYADVSAAVDAINRGGAWRYMTKPWNNEELAQAIHDAVELYTHESQNRRLNEVIRQQNSEMEVWT
ENLKKRLLQSTSTIREQSQALKELSLKKPVAVVLQAFDGFFEIMGGSNAVHARTVSTLVADVARKMGLDAETITRFRLAA
LLHDTGKFGTLVAELNKSMEDMSESEAREYRRHPLRGEELVSRVEELAEVALLVRSHHETFDGQGFPDGLEGERIPLGAR
LIAIADLIERSARSVEKHRADFALMTARFHGGTLLDPHLVTKFQGITRTIYFEGRKSGLASELELEPKDLIPGMQISRDV
ESGAGVLLMQRGTVLDMAGILLIRSHFHKTPSPHGIYVQVTSD

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=121, Percent_Identity=38.8429752066116, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=181, Percent_Identity=28.1767955801105, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 49343; Mature: 49212

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMDMDETHQAGQGPISVLLVDDEEYILTSLRRLLMDEKDMEVVTAGSGEEGLNLLPTLA
CCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
NLGVIVSDQRMPGMNGAQFLEKARLVRPDAVRIILTGYADVSAAVDAINRGGAWRYMTKP
HCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEECCCC
WNNEELAQAIHDAVELYTHESQNRRLNEVIRQQNSEMEVWTENLKKRLLQSTSTIREQSQ
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALKELSLKKPVAVVLQAFDGFFEIMGGSNAVHARTVSTLVADVARKMGLDAETITRFRLA
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ALLHDTGKFGTLVAELNKSMEDMSESEAREYRRHPLRGEELVSRVEELAEVALLVRSHHE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
TFDGQGFPDGLEGERIPLGARLIAIADLIERSARSVEKHRADFALMTARFHGGTLLDPHL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHH
VTKFQGITRTIYFEGRKSGLASELELEPKDLIPGMQISRDVESGAGVLLMQRGTVLDMAG
HHHHCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCEEEEECCCHHHHHH
ILLIRSHFHKTPSPHGIYVQVTSD
HHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure 
AMDMDETHQAGQGPISVLLVDDEEYILTSLRRLLMDEKDMEVVTAGSGEEGLNLLPTLA
CCCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
NLGVIVSDQRMPGMNGAQFLEKARLVRPDAVRIILTGYADVSAAVDAINRGGAWRYMTKP
HCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEECCCC
WNNEELAQAIHDAVELYTHESQNRRLNEVIRQQNSEMEVWTENLKKRLLQSTSTIREQSQ
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALKELSLKKPVAVVLQAFDGFFEIMGGSNAVHARTVSTLVADVARKMGLDAETITRFRLA
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ALLHDTGKFGTLVAELNKSMEDMSESEAREYRRHPLRGEELVSRVEELAEVALLVRSHHE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
TFDGQGFPDGLEGERIPLGARLIAIADLIERSARSVEKHRADFALMTARFHGGTLLDPHL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHH
VTKFQGITRTIYFEGRKSGLASELELEPKDLIPGMQISRDVESGAGVLLMQRGTVLDMAG
HHHHCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCEEEEECCCHHHHHH
ILLIRSHFHKTPSPHGIYVQVTSD
HHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]