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The map label for this gene is zraS [H]

Identifier: 118581129

GI number: 118581129

Start: 2967972

End: 2970023

Strand: Reverse

Name: zraS [H]

Synonym: Ppro_2719

Alternate gene names: 118581129

Gene position: 2970023-2967972 (Counterclockwise)

Preceding gene: 118581130

Following gene: 118581128

Centisome position: 74.1

GC content: 57.99

Gene sequence:

>2052_bases
ATGACTACCACAGATGAACAGAGCGCTCCCATAATCGTCCCCGACAGAGCCGGCCAGTCCGACGCGCGATCGGCCGTGAC
GGCCCGGTTTCTCGGACGCATCATCCAGAGCATCGCTGCCCCGCTCTACGTCATCGACAGGTCCCATGCCATCCTCTTCT
GGAACAGCGCATTGGCGCAATTGACCGGCAAGAGCAGCTTCCAGATGACCGGCACGAAACTGCACTGGAGCGTCTTTCAT
ACCCGGCAGAAACAGCTGCTGGCGGATCTGATCATCGACGGTGCATGCCCGGCGTCCGACCGCGCCTATCCCCAGGTGGT
GCGCCATGCGGACGGAACGCTTCAGACTGAAGGGTGGTACGATGACGTGGGGGGGAAACGACGCTATCTCCTCTTCGAGG
CGAGGCCGATCACGGACGACGGCAATACGGTCGTCGCCGCTGTCGAGACCATTCAGGACATCACCGAACGGAAGCAGGCC
CAGGAAGTCATGGACGGCCAGCGCCGTTTCTTCCAGGGGATCCTGGAGTCCATTCCGAATCCGGTGTCGTACAAGGACCT
CAGTCATGCCTATTTGGGATGCAACAGGGCATTTTCCGAATTTTTCGGAAGAAGCGAAGAAGAGATCATCGGCAAGATGA
TCTCCGATGTCGTTGCGCCGGAATATGCCGTTGGGAGCACCGAGGCCGACCGGAGGGTGCTGGAAAGCGGCTCTTCTTAC
AAATACGAGACGACGTTGCCGCGCGGCGATGGCCAGCTCCGCTCCGTACTGGTCACGAAGGCTCCGTTCTCGCTACTCAA
TGGAGAGTTGGGGGGGACAGTCGGCACATTCGTTGATATTACCGAACAGCGTTTCCTGGATGAACAGGTGAAAGAGGCCA
AGCGGGAGTGGGAGGAAACCCTGGACTGCCTGAAGGATTTCGTCATCCTCACCGATGAACTGCACCGCGTGCGGCGCTGC
AACCGTCTGCTGTGTGACATGACCGGCAAGGGGTTTGATGAGGTGCTGAATGAAGATTGGCGCGACTTGCTCCCCGATGC
GGGGTTCGAGGCCCTGTCGTTCAACGGTGTCAGCGGCGAACTCGTTCATGCCGCCTCGAAACGGTATTACGGTCTCAATA
TCTACAGCATGACCGAAACGGAGAACGAGCGGGTTACCGGATACGTCATTTCACTCAACGACATGACCGAACTCCGCGCC
ATGAACGAAGAGCTGAAGCGGACCGCCCAGGAGCTCAATGAGGCGCAGCGTGCGGTGTATCAGCAGGAAAAACTGGCCTC
CATTGGTCAGCTGGCCGCAGGCGTGGCCCACGAGATCAACAACCCCATGGGCTTCATCTCCAGCAACCTCACGACCCTGG
GAAAGTACCTGGATAAGCTAAGAGCCTTTGAGACCGCCATGATAGGGCTCATGGAGCGTGACGGCAATTCGGAGACCCTC
GGCGAACTAAAGGAGCTGCGACGGAACATGAAAATCGATTATATCCTGGGCGATATCCAGGGGTTGCTGGAGGAGTCTCA
CGAAGGCGCGGAGCGGGTGCGTCGCATCGTCCAGGATCTCAAGAGCTTCTCCCGTGTGGATGAAGCCGAATGCAAGCCGA
CGTGCATCAACGATTGCCTTACCAGCACCCTCAACATGGTGCGTAACGAAATCAAGTATGTCGCCGAAGTGGAGCTTGAC
GTCGCCCCCGACCTCCCTCTCCTGCACTGTTATCCCCAGCAGCTTAACCAGGTGTTCATGAACCTGCTGGTCAACGCGGC
GCATGCCATCACGGAGCGCGGCGTCATCGGTATCAGGACATTTCGTGATGGTGATGATGTCGTTATCCAGGTTCGTGACA
CCGGAAAGGGAATCGCTCCCGAGCACCTGACTCGGGTCTTCGAGCCGTTTTTCACCACCAAGGAGGTTGGCAAGGGTACG
GGGCTTGGGCTCTCCATAAGCTACGATATCGTCAAGAAGCATGGCGGCAGAATTGAAGTGGAGAGCGCCGTTGGCGTGGG
CTCTACCTTTACGATCCATCTTCCTCTCGGCTATCTGGCCGAAACGGTTTGA

Upstream 100 bases:

>100_bases
CGAGCCGGGCAGGGGGAGCACGTTTACGGTGAGGCTTCCGGCCCGGAAGGGTGAGTGATTTCTTTGAATCATAAATGTTA
CGAACGAGGAGAGGTTCTTC

Downstream 100 bases:

>100_bases
GCAGGAAAACGTCGCCGGCGCCACTTCACTGAAAGAAAATGACACATGTTTGAAACGGGTAAACAGCATTCAGCGCCATT
TGTCAGGACTGTAAGAGGAT

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MTTTDEQSAPIIVPDRAGQSDARSAVTARFLGRIIQSIAAPLYVIDRSHAILFWNSALAQLTGKSSFQMTGTKLHWSVFH
TRQKQLLADLIIDGACPASDRAYPQVVRHADGTLQTEGWYDDVGGKRRYLLFEARPITDDGNTVVAAVETIQDITERKQA
QEVMDGQRRFFQGILESIPNPVSYKDLSHAYLGCNRAFSEFFGRSEEEIIGKMISDVVAPEYAVGSTEADRRVLESGSSY
KYETTLPRGDGQLRSVLVTKAPFSLLNGELGGTVGTFVDITEQRFLDEQVKEAKREWEETLDCLKDFVILTDELHRVRRC
NRLLCDMTGKGFDEVLNEDWRDLLPDAGFEALSFNGVSGELVHAASKRYYGLNIYSMTETENERVTGYVISLNDMTELRA
MNEELKRTAQELNEAQRAVYQQEKLASIGQLAAGVAHEINNPMGFISSNLTTLGKYLDKLRAFETAMIGLMERDGNSETL
GELKELRRNMKIDYILGDIQGLLEESHEGAERVRRIVQDLKSFSRVDEAECKPTCINDCLTSTLNMVRNEIKYVAEVELD
VAPDLPLLHCYPQQLNQVFMNLLVNAAHAITERGVIGIRTFRDGDDVVIQVRDTGKGIAPEHLTRVFEPFFTTKEVGKGT
GLGLSISYDIVKKHGGRIEVESAVGVGSTFTIHLPLGYLAETV

Sequences:

>Translated_683_residues
MTTTDEQSAPIIVPDRAGQSDARSAVTARFLGRIIQSIAAPLYVIDRSHAILFWNSALAQLTGKSSFQMTGTKLHWSVFH
TRQKQLLADLIIDGACPASDRAYPQVVRHADGTLQTEGWYDDVGGKRRYLLFEARPITDDGNTVVAAVETIQDITERKQA
QEVMDGQRRFFQGILESIPNPVSYKDLSHAYLGCNRAFSEFFGRSEEEIIGKMISDVVAPEYAVGSTEADRRVLESGSSY
KYETTLPRGDGQLRSVLVTKAPFSLLNGELGGTVGTFVDITEQRFLDEQVKEAKREWEETLDCLKDFVILTDELHRVRRC
NRLLCDMTGKGFDEVLNEDWRDLLPDAGFEALSFNGVSGELVHAASKRYYGLNIYSMTETENERVTGYVISLNDMTELRA
MNEELKRTAQELNEAQRAVYQQEKLASIGQLAAGVAHEINNPMGFISSNLTTLGKYLDKLRAFETAMIGLMERDGNSETL
GELKELRRNMKIDYILGDIQGLLEESHEGAERVRRIVQDLKSFSRVDEAECKPTCINDCLTSTLNMVRNEIKYVAEVELD
VAPDLPLLHCYPQQLNQVFMNLLVNAAHAITERGVIGIRTFRDGDDVVIQVRDTGKGIAPEHLTRVFEPFFTTKEVGKGT
GLGLSISYDIVKKHGGRIEVESAVGVGSTFTIHLPLGYLAETV
>Mature_682_residues
TTTDEQSAPIIVPDRAGQSDARSAVTARFLGRIIQSIAAPLYVIDRSHAILFWNSALAQLTGKSSFQMTGTKLHWSVFHT
RQKQLLADLIIDGACPASDRAYPQVVRHADGTLQTEGWYDDVGGKRRYLLFEARPITDDGNTVVAAVETIQDITERKQAQ
EVMDGQRRFFQGILESIPNPVSYKDLSHAYLGCNRAFSEFFGRSEEEIIGKMISDVVAPEYAVGSTEADRRVLESGSSYK
YETTLPRGDGQLRSVLVTKAPFSLLNGELGGTVGTFVDITEQRFLDEQVKEAKREWEETLDCLKDFVILTDELHRVRRCN
RLLCDMTGKGFDEVLNEDWRDLLPDAGFEALSFNGVSGELVHAASKRYYGLNIYSMTETENERVTGYVISLNDMTELRAM
NEELKRTAQELNEAQRAVYQQEKLASIGQLAAGVAHEINNPMGFISSNLTTLGKYLDKLRAFETAMIGLMERDGNSETLG
ELKELRRNMKIDYILGDIQGLLEESHEGAERVRRIVQDLKSFSRVDEAECKPTCINDCLTSTLNMVRNEIKYVAEVELDV
APDLPLLHCYPQQLNQVFMNLLVNAAHAITERGVIGIRTFRDGDDVVIQVRDTGKGIAPEHLTRVFEPFFTTKEVGKGTG
LGLSISYDIVKKHGGRIEVESAVGVGSTFTIHLPLGYLAETV

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=263, Percent_Identity=34.6007604562738, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1788549, Length=270, Percent_Identity=31.8518518518519, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI145693157, Length=312, Percent_Identity=27.8846153846154, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081816, Length=178, Percent_Identity=33.1460674157303, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI48994928, Length=144, Percent_Identity=29.8611111111111, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790300, Length=344, Percent_Identity=26.7441860465116, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=107, Percent_Identity=36.4485981308411, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76220; Mature: 76089

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTDEQSAPIIVPDRAGQSDARSAVTARFLGRIIQSIAAPLYVIDRSHAILFWNSALAQ
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECHHHHH
LTGKSSFQMTGTKLHWSVFHTRQKQLLADLIIDGACPASDRAYPQVVRHADGTLQTEGWY
HCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEECCCCC
DDVGGKRRYLLFEARPITDDGNTVVAAVETIQDITERKQAQEVMDGQRRFFQGILESIPN
CCCCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVSYKDLSHAYLGCNRAFSEFFGRSEEEIIGKMISDVVAPEYAVGSTEADRRVLESGSSY
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCE
KYETTLPRGDGQLRSVLVTKAPFSLLNGELGGTVGTFVDITEQRFLDEQVKEAKREWEET
EEEECCCCCCCHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDCLKDFVILTDELHRVRRCNRLLCDMTGKGFDEVLNEDWRDLLPDAGFEALSFNGVSGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCH
LVHAASKRYYGLNIYSMTETENERVTGYVISLNDMTELRAMNEELKRTAQELNEAQRAVY
HHHHHCCCEECEEEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QQEKLASIGQLAAGVAHEINNPMGFISSNLTTLGKYLDKLRAFETAMIGLMERDGNSETL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
GELKELRRNMKIDYILGDIQGLLEESHEGAERVRRIVQDLKSFSRVDEAECKPTCINDCL
HHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
TSTLNMVRNEIKYVAEVELDVAPDLPLLHCYPQQLNQVFMNLLVNAAHAITERGVIGIRT
HHHHHHHHHHHHHHHEEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
FRDGDDVVIQVRDTGKGIAPEHLTRVFEPFFTTKEVGKGTGLGLSISYDIVKKHGGRIEV
EECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCEEEE
ESAVGVGSTFTIHLPLGYLAETV
ECCCCCCCEEEEEECHHHHHHCC
>Mature Secondary Structure 
TTTDEQSAPIIVPDRAGQSDARSAVTARFLGRIIQSIAAPLYVIDRSHAILFWNSALAQ
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECHHHHH
LTGKSSFQMTGTKLHWSVFHTRQKQLLADLIIDGACPASDRAYPQVVRHADGTLQTEGWY
HCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEECCCCC
DDVGGKRRYLLFEARPITDDGNTVVAAVETIQDITERKQAQEVMDGQRRFFQGILESIPN
CCCCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVSYKDLSHAYLGCNRAFSEFFGRSEEEIIGKMISDVVAPEYAVGSTEADRRVLESGSSY
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCE
KYETTLPRGDGQLRSVLVTKAPFSLLNGELGGTVGTFVDITEQRFLDEQVKEAKREWEET
EEEECCCCCCCHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDCLKDFVILTDELHRVRRCNRLLCDMTGKGFDEVLNEDWRDLLPDAGFEALSFNGVSGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCH
LVHAASKRYYGLNIYSMTETENERVTGYVISLNDMTELRAMNEELKRTAQELNEAQRAVY
HHHHHCCCEECEEEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QQEKLASIGQLAAGVAHEINNPMGFISSNLTTLGKYLDKLRAFETAMIGLMERDGNSETL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
GELKELRRNMKIDYILGDIQGLLEESHEGAERVRRIVQDLKSFSRVDEAECKPTCINDCL
HHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
TSTLNMVRNEIKYVAEVELDVAPDLPLLHCYPQQLNQVFMNLLVNAAHAITERGVIGIRT
HHHHHHHHHHHHHHHEEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
FRDGDDVVIQVRDTGKGIAPEHLTRVFEPFFTTKEVGKGTGLGLSISYDIVKKHGGRIEV
EECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCEEEE
ESAVGVGSTFTIHLPLGYLAETV
ECCCCCCCEEEEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]