Click here to switch to the map view.

The map label for this gene is appA [H]

Identifier: 118581066

GI number: 118581066

Start: 2888405

End: 2890024

Strand: Reverse

Name: appA [H]

Synonym: Ppro_2655

Alternate gene names: 118581066

Gene position: 2890024-2888405 (Counterclockwise)

Preceding gene: 118581067

Following gene: 118581063

Centisome position: 72.11

GC content: 52.35

Gene sequence:

>1620_bases
ATGAGGTGGCTCTTGCATTGCGGGTGCCTTGTTGCCGTTCTATTTCTGGTTTCCTGTCAGCAGGAAGTGCCCAAGAGAGT
AAGCTCTTCGGCTACGGATGAGAAGCCGGCTATTGGCGATGCAATCATCGAAGGGACCATAGGTGAGGCCACCACGCTGA
TCCCCATTCTTGCCACGGATGCATCCTCCCATGCCATTGCAGCACAACTCTATAACGGCCTAGTAAAATATGATAAAAAC
CTTAATATTGTTGGAGACTTGGCTGAGCGGTTCTCCGTGTCTCCCGACGGTCTCCAGATTACGTTTTACCTCCGCCGCGG
AGTGAAGTGGCATGACGGTTTTCCCTTTACCTCTCGAGATGTGCTCTACACCTATCGCGTCATTATCGATCCCCGCACCC
CCACCGCATACTCAGAAGATTTCAAACAGGTAAAAAGTGTCTCCGCGCTCGATGATTATACGGTGAGAGTGAGCTATGCT
TCCCCTTTTGCTCCGGCACTGGCATCCTGGGGGACGAGCATCCTCCCGGCACACTTGCTGGAGGGTAAGGATATCACGAG
AAGCTCATTGGCGCGTAGGCCGGTCGGTACTGGACCCTATCGCTTCAGGGAGTGGGTTGCCGGGCAGAAAGTAGTTCTGG
ATGCCAATCCTGACTACTTTGAAGGGCGCCCCTACATAAGTCGTTATATCTACCGCATCATTCCCGATACATCCACCATG
TATATGGAACTCAAGTCCGGTTCCATCGACATGATGGGCCTGACACCTGTTCAGTACGCCAGGCAGACGATGACGTCTGA
TTTTATCTCCCGATTCAACAAGTATCGCTACCCCTCATCCAGTTACGTCTATATGGGGTATAACCTGCGCCATCCGCTAT
TCGGAGACAAGCGTATTCGTCAGGCTTTGACGGCAGCCATCAACAAAGACGAGTTGATCCAGGGGGTGCTATTCGGCATG
GGGCGAAAAGCCCATGGCCCGATAATTCCCGGACGCTGGGCATATAATCCCCATGTCAGAGACATTGCCTACGATCCTCA
ACGATCCGCTCAGTTGCTGGCCCAAGCCGGTTGGAGGAGAAAGGATGCCGCGGGGATTCTGGTGAAGAATGGAAAACCAT
TTTCTCTCACCATTCTCACCAATCAAGGAAATCAGCAGCGTCTGCTGACCGCTCAGATCATCCAGCAGCGCCTTAGATTT
GTGGGGGTTGACGCGAAGATCCGGGTCGTGGAGTGGGCCACTTTCTTGAAGGAGTTTGTGGATAAGGGAAATTTCGAGGT
TGTAATGCTTGGATGGAGTACTACACCGGATCCGGACTTATATGATGTCTGGCACTCCAGTAAAACCAGGCCGGGGGAGT
TAAATTTCATCGGTTTCAGAAACGCCGAAGTTGACCGGTTGCTGGGTGAGGGACGCAGTACTTTTGATTTGGAAAAGCGC
AAACGCGCCTATTTTCGTATTCAGGAAATTTTGGCGGATGAACAGCCCTACACATTCCTGTATGTCCCCGACGCGCTTCC
GGTGGTGAGTTCGCGTATTCGTGGCATTGAGCCTGCTCCGGCTGGAATCGGCCACAACCAGATTAGATGGTATGTGCCGA
AGGGTGAACAGCGCTATTGA

Upstream 100 bases:

>100_bases
AGAAGGCCTTAAATGCATATAAGCGGGCGCAGGAGCTTGATCCGAGCCGCAAGGAGCTGACCGAAAAGATTCGCAAGCTC
AAAGGGGAGCTGGGTGAACG

Downstream 100 bases:

>100_bases
TCTTGCGCGTCTGGTACACTGTATGAGGAAATCTTTGACCAATGGCTGGCGCGTTTTGGTGGTGCATTTGATTGAATGAG
GCTGCTGCGGTCCTTTAGGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MRWLLHCGCLVAVLFLVSCQQEVPKRVSSSATDEKPAIGDAIIEGTIGEATTLIPILATDASSHAIAAQLYNGLVKYDKN
LNIVGDLAERFSVSPDGLQITFYLRRGVKWHDGFPFTSRDVLYTYRVIIDPRTPTAYSEDFKQVKSVSALDDYTVRVSYA
SPFAPALASWGTSILPAHLLEGKDITRSSLARRPVGTGPYRFREWVAGQKVVLDANPDYFEGRPYISRYIYRIIPDTSTM
YMELKSGSIDMMGLTPVQYARQTMTSDFISRFNKYRYPSSSYVYMGYNLRHPLFGDKRIRQALTAAINKDELIQGVLFGM
GRKAHGPIIPGRWAYNPHVRDIAYDPQRSAQLLAQAGWRRKDAAGILVKNGKPFSLTILTNQGNQQRLLTAQIIQQRLRF
VGVDAKIRVVEWATFLKEFVDKGNFEVVMLGWSTTPDPDLYDVWHSSKTRPGELNFIGFRNAEVDRLLGEGRSTFDLEKR
KRAYFRIQEILADEQPYTFLYVPDALPVVSSRIRGIEPAPAGIGHNQIRWYVPKGEQRY

Sequences:

>Translated_539_residues
MRWLLHCGCLVAVLFLVSCQQEVPKRVSSSATDEKPAIGDAIIEGTIGEATTLIPILATDASSHAIAAQLYNGLVKYDKN
LNIVGDLAERFSVSPDGLQITFYLRRGVKWHDGFPFTSRDVLYTYRVIIDPRTPTAYSEDFKQVKSVSALDDYTVRVSYA
SPFAPALASWGTSILPAHLLEGKDITRSSLARRPVGTGPYRFREWVAGQKVVLDANPDYFEGRPYISRYIYRIIPDTSTM
YMELKSGSIDMMGLTPVQYARQTMTSDFISRFNKYRYPSSSYVYMGYNLRHPLFGDKRIRQALTAAINKDELIQGVLFGM
GRKAHGPIIPGRWAYNPHVRDIAYDPQRSAQLLAQAGWRRKDAAGILVKNGKPFSLTILTNQGNQQRLLTAQIIQQRLRF
VGVDAKIRVVEWATFLKEFVDKGNFEVVMLGWSTTPDPDLYDVWHSSKTRPGELNFIGFRNAEVDRLLGEGRSTFDLEKR
KRAYFRIQEILADEQPYTFLYVPDALPVVSSRIRGIEPAPAGIGHNQIRWYVPKGEQRY
>Mature_539_residues
MRWLLHCGCLVAVLFLVSCQQEVPKRVSSSATDEKPAIGDAIIEGTIGEATTLIPILATDASSHAIAAQLYNGLVKYDKN
LNIVGDLAERFSVSPDGLQITFYLRRGVKWHDGFPFTSRDVLYTYRVIIDPRTPTAYSEDFKQVKSVSALDDYTVRVSYA
SPFAPALASWGTSILPAHLLEGKDITRSSLARRPVGTGPYRFREWVAGQKVVLDANPDYFEGRPYISRYIYRIIPDTSTM
YMELKSGSIDMMGLTPVQYARQTMTSDFISRFNKYRYPSSSYVYMGYNLRHPLFGDKRIRQALTAAINKDELIQGVLFGM
GRKAHGPIIPGRWAYNPHVRDIAYDPQRSAQLLAQAGWRRKDAAGILVKNGKPFSLTILTNQGNQQRLLTAQIIQQRLRF
VGVDAKIRVVEWATFLKEFVDKGNFEVVMLGWSTTPDPDLYDVWHSSKTRPGELNFIGFRNAEVDRLLGEGRSTFDLEKR
KRAYFRIQEILADEQPYTFLYVPDALPVVSSRIRGIEPAPAGIGHNQIRWYVPKGEQRY

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=508, Percent_Identity=29.1338582677165, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787762, Length=491, Percent_Identity=27.4949083503055, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1787052, Length=481, Percent_Identity=28.0665280665281, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789887, Length=445, Percent_Identity=26.2921348314607, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787551, Length=523, Percent_Identity=26.5774378585086, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787495, Length=532, Percent_Identity=23.6842105263158, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87081878, Length=522, Percent_Identity=23.9463601532567, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1789397, Length=519, Percent_Identity=23.121387283237, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI87082063, Length=414, Percent_Identity=21.9806763285024, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61048; Mature: 61048

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWLLHCGCLVAVLFLVSCQQEVPKRVSSSATDEKPAIGDAIIEGTIGEATTLIPILATD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCEEEEEEEECC
ASSHAIAAQLYNGLVKYDKNLNIVGDLAERFSVSPDGLQITFYLRRGVKWHDGFPFTSRD
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCEECCCCCCCCCC
VLYTYRVIIDPRTPTAYSEDFKQVKSVSALDDYTVRVSYASPFAPALASWGTSILPAHLL
EEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHH
EGKDITRSSLARRPVGTGPYRFREWVAGQKVVLDANPDYFEGRPYISRYIYRIIPDTSTM
CCCCCCHHHHHCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEE
YMELKSGSIDMMGLTPVQYARQTMTSDFISRFNKYRYPSSSYVYMGYNLRHPLFGDKRIR
EEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHH
QALTAAINKDELIQGVLFGMGRKAHGPIIPGRWAYNPHVRDIAYDPQRSAQLLAQAGWRR
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
KDAAGILVKNGKPFSLTILTNQGNQQRLLTAQIIQQRLRFVGVDAKIRVVEWATFLKEFV
CCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
DKGNFEVVMLGWSTTPDPDLYDVWHSSKTRPGELNFIGFRNAEVDRLLGEGRSTFDLEKR
CCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHH
KRAYFRIQEILADEQPYTFLYVPDALPVVSSRIRGIEPAPAGIGHNQIRWYVPKGEQRY
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MRWLLHCGCLVAVLFLVSCQQEVPKRVSSSATDEKPAIGDAIIEGTIGEATTLIPILATD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCEEEEEEEECC
ASSHAIAAQLYNGLVKYDKNLNIVGDLAERFSVSPDGLQITFYLRRGVKWHDGFPFTSRD
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCEECCCCCCCCCC
VLYTYRVIIDPRTPTAYSEDFKQVKSVSALDDYTVRVSYASPFAPALASWGTSILPAHLL
EEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHH
EGKDITRSSLARRPVGTGPYRFREWVAGQKVVLDANPDYFEGRPYISRYIYRIIPDTSTM
CCCCCCHHHHHCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEE
YMELKSGSIDMMGLTPVQYARQTMTSDFISRFNKYRYPSSSYVYMGYNLRHPLFGDKRIR
EEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHH
QALTAAINKDELIQGVLFGMGRKAHGPIIPGRWAYNPHVRDIAYDPQRSAQLLAQAGWRR
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
KDAAGILVKNGKPFSLTILTNQGNQQRLLTAQIIQQRLRFVGVDAKIRVVEWATFLKEFV
CCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
DKGNFEVVMLGWSTTPDPDLYDVWHSSKTRPGELNFIGFRNAEVDRLLGEGRSTFDLEKR
CCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHH
KRAYFRIQEILADEQPYTFLYVPDALPVVSSRIRGIEPAPAGIGHNQIRWYVPKGEQRY
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]