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The map label for this gene is pilT [H]

Identifier: 118580925

GI number: 118580925

Start: 2722080

End: 2723177

Strand: Reverse

Name: pilT [H]

Synonym: Ppro_2513

Alternate gene names: 118580925

Gene position: 2723177-2722080 (Counterclockwise)

Preceding gene: 118580926

Following gene: 118580924

Centisome position: 67.94

GC content: 62.2

Gene sequence:

>1098_bases
ATGCTCAGTCTTCACCAACTGCTCAAGACGCTGGTCGAGGCCAACGGCTCCGACCTGCACATAACCACCAATTCGCCTCC
CCAGATCCGCGTTGACGGCAAACTGCTGCCGCTGGACTATCCCCCCCTCAACCAGGTGGACACCAAGCAGCTCTGCTACA
GCGTGCTCACCGACACCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACCTCTCCTTCGGCGTCAAGGGGTTGTCC
CGCTTCAGGGGCAATGTCTTCGTGCAGCGCGGGGCGGTTGCCGGCGTCTTTCGCGTGATTCCCTACAAGATCCTCTCCTT
CGAGGAACTCTGCCTTCCGTCGATCGTGGCAGATCTGGCCTCCAAGGCCCGCGGTCTGATCCTGGTAACCGGCCCCACCG
GCAGCGGCAAGTCAACGACCCTGGCGTCCATCATCGACTACATCAACGTGAACCGCCACGAGCACATCGTCACCATCGAG
GATCCCATCGAGTACCTCCACCCCCACAAGGGGTGCGTGGTCAACCAGCGCGAGGTGGGCGCCGATACCAAGGGGTTCAA
GAATGCCCTCAAGTACGTGCTGCGCCAGGACCCGGACGTGGTCCTGGTGGGCGAGCTGCGCGACCTGGAGACCATCGAGG
CGGCTCTGACCCTCTCCGAGACCGGTCACCTCTGCCTGGCCACGTTGCACACCAACTCCTGCGTGCAGACCATCAACCGT
ATCGTGGATGTGTTCCCCCCCCACCAGCAGACCCAGATCAGGGCGCAGCTCTCCTTCGTGCTGGAGGGGGTCATCTCCCA
GATGCTGCTTCCCCGGATGAACGCCAAGGGGCGTTCGCTGGTGCTGGAGATCATGGTGCCCAACGCCGCCATCCGCAACC
TGATCCGCGAGGACAAGGTTCACCAGATCTACTCCCAGATGCAGGTGGGCCAGGAAAAGTTCGGCATGCAGACCATGAAC
CAGTCCCTGTTCTCGCTGTACCAGAGACGGCAGATCTCGCTGGAAGATGCCCTGGGTCGGTCGCAGGACCCGGAAGAGTT
GAAACAGATGCTCAGCAACCCGGCAACGAACCCGCAACGCCGTCCCCCTGGCCGCTGA

Upstream 100 bases:

>100_bases
AGGAAGGGGTTACCTCGTTCGAAGAGGTGCTGCGGGTCACCATCGCCGATACCTAAATCCGTTACTGGTGACTGGTCACG
AGTCACGAAAGGCGCATTCC

Downstream 100 bases:

>100_bases
CTTTATTGATCAAGGAGAGTTTTTATGCCCAAGTTTACCTGGGAAGCACGCACCAAAACCGGCGGTGTCCAGAAGGGGAC
CATGGAGGCCGACAGTGTCA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MLSLHQLLKTLVEANGSDLHITTNSPPQIRVDGKLLPLDYPPLNQVDTKQLCYSVLTDTQKHKFEEENELDLSFGVKGLS
RFRGNVFVQRGAVAGVFRVIPYKILSFEELCLPSIVADLASKARGLILVTGPTGSGKSTTLASIIDYINVNRHEHIVTIE
DPIEYLHPHKGCVVNQREVGADTKGFKNALKYVLRQDPDVVLVGELRDLETIEAALTLSETGHLCLATLHTNSCVQTINR
IVDVFPPHQQTQIRAQLSFVLEGVISQMLLPRMNAKGRSLVLEIMVPNAAIRNLIREDKVHQIYSQMQVGQEKFGMQTMN
QSLFSLYQRRQISLEDALGRSQDPEELKQMLSNPATNPQRRPPGR

Sequences:

>Translated_365_residues
MLSLHQLLKTLVEANGSDLHITTNSPPQIRVDGKLLPLDYPPLNQVDTKQLCYSVLTDTQKHKFEEENELDLSFGVKGLS
RFRGNVFVQRGAVAGVFRVIPYKILSFEELCLPSIVADLASKARGLILVTGPTGSGKSTTLASIIDYINVNRHEHIVTIE
DPIEYLHPHKGCVVNQREVGADTKGFKNALKYVLRQDPDVVLVGELRDLETIEAALTLSETGHLCLATLHTNSCVQTINR
IVDVFPPHQQTQIRAQLSFVLEGVISQMLLPRMNAKGRSLVLEIMVPNAAIRNLIREDKVHQIYSQMQVGQEKFGMQTMN
QSLFSLYQRRQISLEDALGRSQDPEELKQMLSNPATNPQRRPPGR
>Mature_365_residues
MLSLHQLLKTLVEANGSDLHITTNSPPQIRVDGKLLPLDYPPLNQVDTKQLCYSVLTDTQKHKFEEENELDLSFGVKGLS
RFRGNVFVQRGAVAGVFRVIPYKILSFEELCLPSIVADLASKARGLILVTGPTGSGKSTTLASIIDYINVNRHEHIVTIE
DPIEYLHPHKGCVVNQREVGADTKGFKNALKYVLRQDPDVVLVGELRDLETIEAALTLSETGHLCLATLHTNSCVQTINR
IVDVFPPHQQTQIRAQLSFVLEGVISQMLLPRMNAKGRSLVLEIMVPNAAIRNLIREDKVHQIYSQMQVGQEKFGMQTMN
QSLFSLYQRRQISLEDALGRSQDPEELKQMLSNPATNPQRRPPGR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=39.8176291793313, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1786296, Length=287, Percent_Identity=34.1463414634146, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789723, Length=286, Percent_Identity=32.1678321678322, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40844; Mature: 40844

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLHQLLKTLVEANGSDLHITTNSPPQIRVDGKLLPLDYPPLNQVDTKQLCYSVLTDTQ
CCCHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEECCCCCCCCCCCHHHHHHHHHHCHH
KHKFEEENELDLSFGVKGLSRFRGNVFVQRGAVAGVFRVIPYKILSFEELCLPSIVADLA
HHHCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKARGLILVTGPTGSGKSTTLASIIDYINVNRHEHIVTIEDPIEYLHPHKGCVVNQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCEECHHHCC
ADTKGFKNALKYVLRQDPDVVLVGELRDLETIEAALTLSETGHLCLATLHTNSCVQTINR
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
IVDVFPPHQQTQIRAQLSFVLEGVISQMLLPRMNAKGRSLVLEIMVPNAAIRNLIREDKV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HQIYSQMQVGQEKFGMQTMNQSLFSLYQRRQISLEDALGRSQDPEELKQMLSNPATNPQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCCCCCCC
RPPGR
CCCCC
>Mature Secondary Structure
MLSLHQLLKTLVEANGSDLHITTNSPPQIRVDGKLLPLDYPPLNQVDTKQLCYSVLTDTQ
CCCHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEECCCCCCCCCCCHHHHHHHHHHCHH
KHKFEEENELDLSFGVKGLSRFRGNVFVQRGAVAGVFRVIPYKILSFEELCLPSIVADLA
HHHCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKARGLILVTGPTGSGKSTTLASIIDYINVNRHEHIVTIEDPIEYLHPHKGCVVNQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCEECHHHCC
ADTKGFKNALKYVLRQDPDVVLVGELRDLETIEAALTLSETGHLCLATLHTNSCVQTINR
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
IVDVFPPHQQTQIRAQLSFVLEGVISQMLLPRMNAKGRSLVLEIMVPNAAIRNLIREDKV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HQIYSQMQVGQEKFGMQTMNQSLFSLYQRRQISLEDALGRSQDPEELKQMLSNPATNPQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCCCCCCC
RPPGR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]