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The map label for this gene is cyaD [H]

Identifier: 118580782

GI number: 118580782

Start: 2561405

End: 2562748

Strand: Reverse

Name: cyaD [H]

Synonym: Ppro_2368

Alternate gene names: 118580782

Gene position: 2562748-2561405 (Counterclockwise)

Preceding gene: 118580783

Following gene: 118580781

Centisome position: 63.94

GC content: 61.76

Gene sequence:

>1344_bases
ATGAGTATCAGGCAGAGAATGGCAGCCTTCGGCGATGTGCTCAGCCGCTTCAAAACGATTTTCAGCCATTGCTGGCGCAA
CCGCCATCAGCTCGAGGGTAAAGTGCTGGCTGCCCAGGAAGCCGAGTTCCTGCCGGCAGCCCTGTCCCTGCAGGAAAAGC
CGGTTTCGCCGGTCGCACGCCTGCTGGCAAAGGTTCTGATGGCACTGGTGGTTGTTCTGATCACCTGGTCGATCGTGGGC
AAGATCGACATCATCGTCAATGCTTCAGGAAAGATCATTCCCAGCGGCTACACCAAGACCATCGCCTGCGTGGACGTGGC
CAGCGTGAGAGCCCTGTACGTTGCAGAGGGACAGCAGGTCAGGGCAGGAGACCTGTTGATAGAGCTGGATGCCATCGCAT
CCGATGCCGAGCGGGATAAGGCGGCCGGGGATCGCGCCGTGTCGATCCTCCAGGTTGCCTGCGCCAGGTCGCTGCTCGCG
GCAATCGACGGCGGCAGTCCACCGCGGCTTCCTCCCATCGCCACTGTTCCGGCGGATCAATGGCTGGCCGCGAAACAGCA
GCTTGATGCCCAGTACGGCGATTATCAGGCCAGGCTGAAGCGGATCGACGGCGACATCGAGCGCTTCAGCCTGGCGTTGC
AGTTGGCCACGCAACGCGCCACCGACTACAAGGCGCTTTTGAAAGATCGCGACGTCTCCTACCACGCCTGGCTGGAGAAG
GAGCAGGCCCGCGTCGATCTCGCCGGCCAGTTGGCCGACGCCAGAAACCAGCGCAATGCCCTGGTCGCCGAGACCAGGCG
TGTGGCCCATGAACAGTCCACCGAGGGGAGTAAAACCGCGGCGTCTTCTCAGCAGGACGCCATCCGCGCGGCCGCCCACA
GCAGGCTCCTGAAACTCACCGCGCCGGTTAGTGGTACGGTGCAGCAGTTGAACGTGCATACCATCGGCGGTGTGGTTGCC
GCGGCCAGCCCCCTGATGCAGATCGTCCCCAGGGAGAGCCGGGTGGAGGTCGAGGCGTTCATGGAGAACAAGGATGTGGG
CTTTGTCCAGGAGGGGCAGAGCGCCGAGGTCAAGATAGATGCCTTTGAATACACCAAATACGGCACCATAAAGGGAAGAG
TCACGCACGTTTCCCGCGACGCCATCGCCGACGAGAAGCGGGGCCTGATCTATTCCACGAAGATCCGACTGGACAGATCC
TCCATCACTGTGGACGGCAGGGAGATGCCTCTGTCGGCCGGGATGACGGTCAACGTGGAGATCAAGACCGGCGACCGCCG
CATCATCGAATACGTCCTGAGCCCGCTTTTGCGCCACAAACGGGAAAGCCTCAATGAACGGTAG

Upstream 100 bases:

>100_bases
ACCAGGATCGGTCCGCGCACGTGATGCCGACTCCAATGCCTACCGCGACTCCGTCGCCCATACGCTATGCTCCGGTAGTC
AACGCAGCAGGTGAAATAGC

Downstream 100 bases:

>100_bases
GCGGTTTCGGAGACATCGGCCAGTGGCAGGCGGAGCGGCACCGGGCCGCGCCATGCTTTCGCTGATCGCAATCGCATCCA
TTGCTCTCTTTGCTTCATTG

Product: HlyD family type I secretion membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSIRQRMAAFGDVLSRFKTIFSHCWRNRHQLEGKVLAAQEAEFLPAALSLQEKPVSPVARLLAKVLMALVVVLITWSIVG
KIDIIVNASGKIIPSGYTKTIACVDVASVRALYVAEGQQVRAGDLLIELDAIASDAERDKAAGDRAVSILQVACARSLLA
AIDGGSPPRLPPIATVPADQWLAAKQQLDAQYGDYQARLKRIDGDIERFSLALQLATQRATDYKALLKDRDVSYHAWLEK
EQARVDLAGQLADARNQRNALVAETRRVAHEQSTEGSKTAASSQQDAIRAAAHSRLLKLTAPVSGTVQQLNVHTIGGVVA
AASPLMQIVPRESRVEVEAFMENKDVGFVQEGQSAEVKIDAFEYTKYGTIKGRVTHVSRDAIADEKRGLIYSTKIRLDRS
SITVDGREMPLSAGMTVNVEIKTGDRRIIEYVLSPLLRHKRESLNER

Sequences:

>Translated_447_residues
MSIRQRMAAFGDVLSRFKTIFSHCWRNRHQLEGKVLAAQEAEFLPAALSLQEKPVSPVARLLAKVLMALVVVLITWSIVG
KIDIIVNASGKIIPSGYTKTIACVDVASVRALYVAEGQQVRAGDLLIELDAIASDAERDKAAGDRAVSILQVACARSLLA
AIDGGSPPRLPPIATVPADQWLAAKQQLDAQYGDYQARLKRIDGDIERFSLALQLATQRATDYKALLKDRDVSYHAWLEK
EQARVDLAGQLADARNQRNALVAETRRVAHEQSTEGSKTAASSQQDAIRAAAHSRLLKLTAPVSGTVQQLNVHTIGGVVA
AASPLMQIVPRESRVEVEAFMENKDVGFVQEGQSAEVKIDAFEYTKYGTIKGRVTHVSRDAIADEKRGLIYSTKIRLDRS
SITVDGREMPLSAGMTVNVEIKTGDRRIIEYVLSPLLRHKRESLNER
>Mature_446_residues
SIRQRMAAFGDVLSRFKTIFSHCWRNRHQLEGKVLAAQEAEFLPAALSLQEKPVSPVARLLAKVLMALVVVLITWSIVGK
IDIIVNASGKIIPSGYTKTIACVDVASVRALYVAEGQQVRAGDLLIELDAIASDAERDKAAGDRAVSILQVACARSLLAA
IDGGSPPRLPPIATVPADQWLAAKQQLDAQYGDYQARLKRIDGDIERFSLALQLATQRATDYKALLKDRDVSYHAWLEKE
QARVDLAGQLADARNQRNALVAETRRVAHEQSTEGSKTAASSQQDAIRAAAHSRLLKLTAPVSGTVQQLNVHTIGGVVAA
ASPLMQIVPRESRVEVEAFMENKDVGFVQEGQSAEVKIDAFEYTKYGTIKGRVTHVSRDAIADEKRGLIYSTKIRLDRSS
ITVDGREMPLSAGMTVNVEIKTGDRRIIEYVLSPLLRHKRESLNER

Specific function: CyaD is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR010129 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48991; Mature: 48860

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00543 HLYD_FAMILY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRQRMAAFGDVLSRFKTIFSHCWRNRHQLEGKVLAAQEAEFLPAALSLQEKPVSPVAR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEECCHHHHHHHHCCCCCCCCHHHH
LLAKVLMALVVVLITWSIVGKIDIIVNASGKIIPSGYTKTIACVDVASVRALYVAEGQQV
HHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEECCCCCCEEEEEEHHHHHEEEEECCCEE
RAGDLLIELDAIASDAERDKAAGDRAVSILQVACARSLLAAIDGGSPPRLPPIATVPADQ
ECCCEEEEEHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHH
WLAAKQQLDAQYGDYQARLKRIDGDIERFSLALQLATQRATDYKALLKDRDVSYHAWLEK
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EQARVDLAGQLADARNQRNALVAETRRVAHEQSTEGSKTAASSQQDAIRAAAHSRLLKLT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHEEEE
APVSGTVQQLNVHTIGGVVAAASPLMQIVPRESRVEVEAFMENKDVGFVQEGQSAEVKID
CCCCCCHHHEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEECCCCCEEEEE
AFEYTKYGTIKGRVTHVSRDAIADEKRGLIYSTKIRLDRSSITVDGREMPLSAGMTVNVE
EEEEEEECCCCEEEEEEHHHHHHHHCCCCEEEEEEEEECCEEEECCCCCCCCCCCEEEEE
IKTGDRRIIEYVLSPLLRHKRESLNER
EECCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIRQRMAAFGDVLSRFKTIFSHCWRNRHQLEGKVLAAQEAEFLPAALSLQEKPVSPVAR
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEECCHHHHHHHHCCCCCCCCHHHH
LLAKVLMALVVVLITWSIVGKIDIIVNASGKIIPSGYTKTIACVDVASVRALYVAEGQQV
HHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEECCCCCCEEEEEEHHHHHEEEEECCCEE
RAGDLLIELDAIASDAERDKAAGDRAVSILQVACARSLLAAIDGGSPPRLPPIATVPADQ
ECCCEEEEEHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHH
WLAAKQQLDAQYGDYQARLKRIDGDIERFSLALQLATQRATDYKALLKDRDVSYHAWLEK
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EQARVDLAGQLADARNQRNALVAETRRVAHEQSTEGSKTAASSQQDAIRAAAHSRLLKLT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHEEEE
APVSGTVQQLNVHTIGGVVAAASPLMQIVPRESRVEVEAFMENKDVGFVQEGQSAEVKID
CCCCCCHHHEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEECCCCCEEEEE
AFEYTKYGTIKGRVTHVSRDAIADEKRGLIYSTKIRLDRSSITVDGREMPLSAGMTVNVE
EEEEEEECCCCEEEEEEHHHHHHHHCCCCEEEEEEEEECCEEEECCCCCCCCCCCEEEEE
IKTGDRRIIEYVLSPLLRHKRESLNER
EECCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]