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The map label for this gene is cyaE [H]

Identifier: 118580781

GI number: 118580781

Start: 2559928

End: 2561415

Strand: Reverse

Name: cyaE [H]

Synonym: Ppro_2367

Alternate gene names: 118580781

Gene position: 2561415-2559928 (Counterclockwise)

Preceding gene: 118580782

Following gene: 118580780

Centisome position: 63.91

GC content: 61.96

Gene sequence:

>1488_bases
ATGAACGGTAGGCGGTTTCGGAGACATCGGCCAGTGGCAGGCGGAGCGGCACCGGGCCGCGCCATGCTTTCGCTGATCGC
AATCGCATCCATTGCTCTCTTTGCTTCATTGCCCGCCCTGGCCTTTGACCCTGCGGCGCCCTTTGACGATCCGCTGCGCA
CCCTTCCCGGAAGGATTGCGTCCGGAACAACCCTGCCCGGCGACGGCGGACCGGTTGCCTGTCCGGTCACGCGGGACCTT
GCGGCACCCTTGAGTCTCATCGATGCCGCTGATCTGGCGTTGTGCAACAACCCCCAGATCAAGGCGGCATGGGCGGCCAT
CAAGCTCCAGTCCGCCGAGGTGGGGCAGGCGCGTGCCGCGTATCTGCCCACCCTGTCCGGTACCATCAACTTCATGAGAA
CGCACTACTCCTATCCGGGATCGGGTGTTGCCTCGACAACCGAGGATGGCACAACCATCAACGCCACCCTGGGGTGGCGG
CTCTTCGACTTCGGGGGGAGGGCGGCCAACCGGGAATCGGCCAACAAGATGCTGGTTGCCGCACTGGCGCAGCACGATGC
CGCACTGCAGAAGAAACTGGCCGATGTCATTCAATCCTATTTTGATGCCGTCACGGCACAGGCCAGCCTGCATGCGAAAG
AGCAGAACGAGTCAATCGCAGTGAATACCCTCGAAACGGCCCAGCGGCGGGAGGATCGCGGTGTTGTGTCGCGCAGCGAC
AGGCTGCAGGCGGCGACGGCCCTGGCCAGGGCAACCCTTGACAGGAATCGGGCCCAGGGAGCCTATCAAAAGGCCGTGGC
GGTTCTGGTGTACGTCCTCGGTATTCCTACCCGGACGGCTATTGCCCTTCCGGACGAATTGAACGGTGATTTCGCGGAAA
GTGAGGAGTCCAAGACGTTGGACGACTGGTTGAAACTGGCGGCAAAAACCCATCCGTCGCTCCTAGCCGGGCGGGCGCAG
TGGGAATCATCGGTGCGCAAGATAGCGGCAACCCGCTCCGAGGGATTGCCGACGGTGGACTTCTCCGCCAACTATTACCA
GAATGGCTACCCCGGCCAGGGGCTGTCGCGTACCGACTCACGGATCACCACCATCGGACTCTCCCTGACGTTCCCCTTCT
TCGACGGCTTTTCGAGGACCTACAAAATTCGGAATGCCGAAGCCCAGGCGGAACAGCAACACGCCGAAGTGCAGGAAACC
GAGCAGAACACACTGATGGAAGTAGTCAAGGCCCATGCCGATGCCGGCTCATCGCTCCAGAATCTTCAGGCATCGAAGAA
ACTCCTCGATATCGCCGAGGCATCCCTGGCTGTCTCGCAACGGAAATACGAGAAAGGGGCCGCCGATATCCTGGAAGTCC
TCAACACTCAGGCGGCGTTGGCCGATGCGCGGCAGGAGCGGATTCGCAGCCTTGCCGAGTGGCGTTCAGCCAAGCTGCGT
CTGCTGGCCTGTGTCGGTGTTCTGGGACGCGGGGAACTGGAGAGGTGA

Upstream 100 bases:

>100_bases
TCGGCCGGGATGACGGTCAACGTGGAGATCAAGACCGGCGACCGCCGCATCATCGAATACGTCCTGAGCCCGCTTTTGCG
CCACAAACGGGAAAGCCTCA

Downstream 100 bases:

>100_bases
ACAGTGCCGAGTCCCTGATAACGGAGGAACGCTGATGCGTTTTCTGTTTGCCTGGGAGCAGGGGGGGCGTCTCGGGCATC
TTTCTAAAATAGTGCCCATT

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNGRRFRRHRPVAGGAAPGRAMLSLIAIASIALFASLPALAFDPAAPFDDPLRTLPGRIASGTTLPGDGGPVACPVTRDL
AAPLSLIDAADLALCNNPQIKAAWAAIKLQSAEVGQARAAYLPTLSGTINFMRTHYSYPGSGVASTTEDGTTINATLGWR
LFDFGGRAANRESANKMLVAALAQHDAALQKKLADVIQSYFDAVTAQASLHAKEQNESIAVNTLETAQRREDRGVVSRSD
RLQAATALARATLDRNRAQGAYQKAVAVLVYVLGIPTRTAIALPDELNGDFAESEESKTLDDWLKLAAKTHPSLLAGRAQ
WESSVRKIAATRSEGLPTVDFSANYYQNGYPGQGLSRTDSRITTIGLSLTFPFFDGFSRTYKIRNAEAQAEQQHAEVQET
EQNTLMEVVKAHADAGSSLQNLQASKKLLDIAEASLAVSQRKYEKGAADILEVLNTQAALADARQERIRSLAEWRSAKLR
LLACVGVLGRGELER

Sequences:

>Translated_495_residues
MNGRRFRRHRPVAGGAAPGRAMLSLIAIASIALFASLPALAFDPAAPFDDPLRTLPGRIASGTTLPGDGGPVACPVTRDL
AAPLSLIDAADLALCNNPQIKAAWAAIKLQSAEVGQARAAYLPTLSGTINFMRTHYSYPGSGVASTTEDGTTINATLGWR
LFDFGGRAANRESANKMLVAALAQHDAALQKKLADVIQSYFDAVTAQASLHAKEQNESIAVNTLETAQRREDRGVVSRSD
RLQAATALARATLDRNRAQGAYQKAVAVLVYVLGIPTRTAIALPDELNGDFAESEESKTLDDWLKLAAKTHPSLLAGRAQ
WESSVRKIAATRSEGLPTVDFSANYYQNGYPGQGLSRTDSRITTIGLSLTFPFFDGFSRTYKIRNAEAQAEQQHAEVQET
EQNTLMEVVKAHADAGSSLQNLQASKKLLDIAEASLAVSQRKYEKGAADILEVLNTQAALADARQERIRSLAEWRSAKLR
LLACVGVLGRGELER
>Mature_495_residues
MNGRRFRRHRPVAGGAAPGRAMLSLIAIASIALFASLPALAFDPAAPFDDPLRTLPGRIASGTTLPGDGGPVACPVTRDL
AAPLSLIDAADLALCNNPQIKAAWAAIKLQSAEVGQARAAYLPTLSGTINFMRTHYSYPGSGVASTTEDGTTINATLGWR
LFDFGGRAANRESANKMLVAALAQHDAALQKKLADVIQSYFDAVTAQASLHAKEQNESIAVNTLETAQRREDRGVVSRSD
RLQAATALARATLDRNRAQGAYQKAVAVLVYVLGIPTRTAIALPDELNGDFAESEESKTLDDWLKLAAKTHPSLLAGRAQ
WESSVRKIAATRSEGLPTVDFSANYYQNGYPGQGLSRTDSRITTIGLSLTFPFFDGFSRTYKIRNAEAQAEQQHAEVQET
EQNTLMEVVKAHADAGSSLQNLQASKKLLDIAEASLAVSQRKYEKGAADILEVLNTQAALADARQERIRSLAEWRSAKLR
LLACVGVLGRGELER

Specific function: CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53151; Mature: 53151

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGRRFRRHRPVAGGAAPGRAMLSLIAIASIALFASLPALAFDPAAPFDDPLRTLPGRIA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHCCCCCC
SGTTLPGDGGPVACPVTRDLAAPLSLIDAADLALCNNPQIKAAWAAIKLQSAEVGQARAA
CCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEEECCCCCCCHHHH
YLPTLSGTINFMRTHYSYPGSGVASTTEDGTTINATLGWRLFDFGGRAANRESANKMLVA
HCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECEEEEECCCCCCCHHHHHHHHHH
ALAQHDAALQKKLADVIQSYFDAVTAQASLHAKEQNESIAVNTLETAQRREDRGVVSRSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCHHH
RLQAATALARATLDRNRAQGAYQKAVAVLVYVLGIPTRTAIALPDELNGDFAESEESKTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCCCCCCCCCCH
DDWLKLAAKTHPSLLAGRAQWESSVRKIAATRSEGLPTVDFSANYYQNGYPGQGLSRTDS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCC
RITTIGLSLTFPFFDGFSRTYKIRNAEAQAEQQHAEVQETEQNTLMEVVKAHADAGSSLQ
EEEEEEEEEEEHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
NLQASKKLLDIAEASLAVSQRKYEKGAADILEVLNTQAALADARQERIRSLAEWRSAKLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLACVGVLGRGELER
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MNGRRFRRHRPVAGGAAPGRAMLSLIAIASIALFASLPALAFDPAAPFDDPLRTLPGRIA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHCCCCCC
SGTTLPGDGGPVACPVTRDLAAPLSLIDAADLALCNNPQIKAAWAAIKLQSAEVGQARAA
CCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEEECCCCCCCHHHH
YLPTLSGTINFMRTHYSYPGSGVASTTEDGTTINATLGWRLFDFGGRAANRESANKMLVA
HCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECEEEEECCCCCCCHHHHHHHHHH
ALAQHDAALQKKLADVIQSYFDAVTAQASLHAKEQNESIAVNTLETAQRREDRGVVSRSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCHHH
RLQAATALARATLDRNRAQGAYQKAVAVLVYVLGIPTRTAIALPDELNGDFAESEESKTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCCCCCCCCCCH
DDWLKLAAKTHPSLLAGRAQWESSVRKIAATRSEGLPTVDFSANYYQNGYPGQGLSRTDS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCC
RITTIGLSLTFPFFDGFSRTYKIRNAEAQAEQQHAEVQETEQNTLMEVVKAHADAGSSLQ
EEEEEEEEEEEHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
NLQASKKLLDIAEASLAVSQRKYEKGAADILEVLNTQAALADARQERIRSLAEWRSAKLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLACVGVLGRGELER
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]