Click here to switch to the map view.

The map label for this gene is mdh [H]

Identifier: 118580742

GI number: 118580742

Start: 2512327

End: 2513280

Strand: Reverse

Name: mdh [H]

Synonym: Ppro_2327

Alternate gene names: 118580742

Gene position: 2513280-2512327 (Counterclockwise)

Preceding gene: 118580743

Following gene: 118580741

Centisome position: 62.71

GC content: 58.7

Gene sequence:

>954_bases
ATGTCGCGTAAAAAGATTGCACTTATCGGCGGTGGACAAATCGGTGGCGTTCTTGCTCAGCTCTGCGCTCTGCGTGAGCT
CGGCGATGTTGTTCTCTTTGACGTTGTTGAAGGCCTTCCCCAAGGCAAGACCCTGGATATCTCCGAGGCAGCGCCGGTGG
ACAATTTCGACGTCTGCCTGAAGGGCGCCAACTCCTATGAAGAAATCGCCGGTTCCAACGTGATCATCATCACCGCCGGT
CTGCCCCGCAAACCGGGCATGAGCCGCGACGACCTGATCGCCACCAACTCCAAAATCATGACGTCGGTTGCCGAAGGCAT
CAAGAAGCATGCTCCCGACGCATTCATCATCTGCATCTCCAACCCGCTGGACGCCATGGTTACCCTCTGTCAGAAAGTCA
CCGGCTTTCCCAAAAACCGCGTCATGGGTATGGCCGGCGTGCTGGATTCGGCCCGCTTCGCCGCCTTCATCGCCTGGGAG
CTGGGTGTTTCCGTGAAGGATGTCACCGCCATGGTCATGGGCGGCCACGGCGACGACATGGTGCCTCTGATCCGTTATGC
CAACGTGTTCGGTATCCCGGTCATGGCTCTGCTTGAGAAGAAATACGGCAGCGCCACCAAGGCCAAGGAAGTCATGGACG
CCATGGTCAAGCGCACCGCCGGCGCCGGCGGCGAAGTTGTCGGCCTGCTCAAGACCGGTTCCGCATTCTACTCCCCGGCT
TCTTCGGCCATCTGCATGGCAGAAGCGATCCTGCGCGACCAGAAACGCGTCCTGCCGGTGTGCGCACTGCTGGAAGGCGA
GTATGGCGTCGACGGCTACTATGTCGGCGTTCCCTGTATCCTCGGTGCCAATGGTGTAGAGAAGATCGTTGAAATCGATC
TGGACGAGACCGAGCAGGCCCTGTTCAACAGCTCCGTTTCCCACGTAAAAGAGCTGGTTGACAGCCTGAAGTAA

Upstream 100 bases:

>100_bases
TGTAAAAAACAAACACAGGCGGGTAATTCTATTCTTGCCATCCATGGGTTGTTGGTTTATAGTGAGTCGTTCGATTTTAC
CTAATCTAGGAGGAAACAGT

Downstream 100 bases:

>100_bases
TCCCGTTGTTCCCGGCGTCGCGGTGGTGGCAATCGCTCTGCCACCGCGACGCAATTCTCGATGAACATCAGTAGAAACGC
AACGACCGCCCCCCGTCCCA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSRKKIALIGGGQIGGVLAQLCALRELGDVVLFDVVEGLPQGKTLDISEAAPVDNFDVCLKGANSYEEIAGSNVIIITAG
LPRKPGMSRDDLIATNSKIMTSVAEGIKKHAPDAFIICISNPLDAMVTLCQKVTGFPKNRVMGMAGVLDSARFAAFIAWE
LGVSVKDVTAMVMGGHGDDMVPLIRYANVFGIPVMALLEKKYGSATKAKEVMDAMVKRTAGAGGEVVGLLKTGSAFYSPA
SSAICMAEAILRDQKRVLPVCALLEGEYGVDGYYVGVPCILGANGVEKIVEIDLDETEQALFNSSVSHVKELVDSLK

Sequences:

>Translated_317_residues
MSRKKIALIGGGQIGGVLAQLCALRELGDVVLFDVVEGLPQGKTLDISEAAPVDNFDVCLKGANSYEEIAGSNVIIITAG
LPRKPGMSRDDLIATNSKIMTSVAEGIKKHAPDAFIICISNPLDAMVTLCQKVTGFPKNRVMGMAGVLDSARFAAFIAWE
LGVSVKDVTAMVMGGHGDDMVPLIRYANVFGIPVMALLEKKYGSATKAKEVMDAMVKRTAGAGGEVVGLLKTGSAFYSPA
SSAICMAEAILRDQKRVLPVCALLEGEYGVDGYYVGVPCILGANGVEKIVEIDLDETEQALFNSSVSHVKELVDSLK
>Mature_316_residues
SRKKIALIGGGQIGGVLAQLCALRELGDVVLFDVVEGLPQGKTLDISEAAPVDNFDVCLKGANSYEEIAGSNVIIITAGL
PRKPGMSRDDLIATNSKIMTSVAEGIKKHAPDAFIICISNPLDAMVTLCQKVTGFPKNRVMGMAGVLDSARFAAFIAWEL
GVSVKDVTAMVMGGHGDDMVPLIRYANVFGIPVMALLEKKYGSATKAKEVMDAMVKRTAGAGGEVVGLLKTGSAFYSPAS
SAICMAEAILRDQKRVLPVCALLEGEYGVDGYYVGVPCILGANGVEKIVEIDLDETEQALFNSSVSHVKELVDSLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=319, Percent_Identity=32.6018808777429, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI221136809, Length=319, Percent_Identity=32.6018808777429, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI15082234, Length=321, Percent_Identity=30.2180685358255, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=32.1311475409836, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=32.1311475409836, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI9257228, Length=306, Percent_Identity=32.0261437908497, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4504973, Length=306, Percent_Identity=32.0261437908497, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI291575128, Length=321, Percent_Identity=28.0373831775701, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557032, Length=321, Percent_Identity=28.0373831775701, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI260099727, Length=220, Percent_Identity=31.8181818181818, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=32.6530612244898, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI207028494, Length=188, Percent_Identity=34.5744680851064, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI21735621, Length=249, Percent_Identity=26.9076305220884, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789632, Length=297, Percent_Identity=29.96632996633, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=280, Percent_Identity=26.4285714285714, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17554310, Length=316, Percent_Identity=31.3291139240506, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6322765, Length=308, Percent_Identity=28.8961038961039, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=282, Percent_Identity=27.3049645390071, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=230, Percent_Identity=29.5652173913043, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17136226, Length=305, Percent_Identity=30.4918032786885, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24647881, Length=254, Percent_Identity=27.9527559055118, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45550422, Length=303, Percent_Identity=27.7227722772277, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24663599, Length=321, Percent_Identity=27.4143302180685, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24663595, Length=230, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33443; Mature: 33312

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00760 SPASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKKIALIGGGQIGGVLAQLCALRELGDVVLFDVVEGLPQGKTLDISEAAPVDNFDVCL
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHEE
KGANSYEEIAGSNVIIITAGLPRKPGMSRDDLIATNSKIMTSVAEGIKKHAPDAFIICIS
ECCCCHHHHCCCCEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEEEC
NPLDAMVTLCQKVTGFPKNRVMGMAGVLDSARFAAFIAWELGVSVKDVTAMVMGGHGDDM
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCH
VPLIRYANVFGIPVMALLEKKYGSATKAKEVMDAMVKRTAGAGGEVVGLLKTGSAFYSPA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHCCCC
SSAICMAEAILRDQKRVLPVCALLEGEYGVDGYYVGVPCILGANGVEKIVEIDLDETEQA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCHHHHHHCCCCCHHHHH
LFNSSVSHVKELVDSLK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRKKIALIGGGQIGGVLAQLCALRELGDVVLFDVVEGLPQGKTLDISEAAPVDNFDVCL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHEE
KGANSYEEIAGSNVIIITAGLPRKPGMSRDDLIATNSKIMTSVAEGIKKHAPDAFIICIS
ECCCCHHHHCCCCEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEEEC
NPLDAMVTLCQKVTGFPKNRVMGMAGVLDSARFAAFIAWELGVSVKDVTAMVMGGHGDDM
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCH
VPLIRYANVFGIPVMALLEKKYGSATKAKEVMDAMVKRTAGAGGEVVGLLKTGSAFYSPA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHCCCC
SSAICMAEAILRDQKRVLPVCALLEGEYGVDGYYVGVPCILGANGVEKIVEIDLDETEQA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCHHHHHHCCCCCHHHHH
LFNSSVSHVKELVDSLK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA