Click here to switch to the map view.

The map label for this gene is traC [H]

Identifier: 118580546

GI number: 118580546

Start: 2288929

End: 2289762

Strand: Reverse

Name: traC [H]

Synonym: Ppro_2130

Alternate gene names: 118580546

Gene position: 2289762-2288929 (Counterclockwise)

Preceding gene: 118580547

Following gene: 118580545

Centisome position: 57.13

GC content: 57.43

Gene sequence:

>834_bases
ATGAACAACCCCATTGACGGATTCAGGCAGTTCATGAGGAACGCCGGGCTTGAACCTCCTGGCGAGATCGTAGCGAACGG
AAGCCTTAACCGGTTCACTGTTCCCGGTGACAAACCGCGGAGCCTCAACGGCTGGTATGTATTCCATGCTGACACCCCGG
CGGCCGGCTCATTCGGTTGCTGGAAACGGCAGCTCAACGAGACATGGAGCGCCAAGGAATACCACAGCATGGACCCGGCA
GAGAAAGCCGCATACAGCGCCAAAATGGAGACCATAAAGCGGCAGCGGGAGGAAGAGCAGGTGCGGATCCATGCTGAGTG
CCGGGCATGGTGCGCCGATGTATGGGGCAAGGCCGAAGACGCCACCAACGATCACCCGTATCTCAAGGCCAAGGGAGTCA
AGTCCTTCGGGCTGAAGTGTTTCAGAGGTAGCCTGTTGATTCCCATTCAGGACATCGCGGGGAATATCTACGGAATGCAG
TTCATCCGGCCGGACGGTTCCAAAACATTCAAGACCGGCACCGCCAAACGAGGACATTTCTACAGCATGGGAGAGGGTAA
AGGCAACACGCTTTGCATTGCTGAAGGTTACGCAACGGCGGCAAGCATTCATCAGGCGACCGGCTACCCGGTTCTTACGG
CTTTCGACGCCGGAAATCTGAAGCCGGTTGCCGAAAACGTCAGAGCGAAGGTCCCGCAGCTGAACATCATCATCTGTGCC
GACAATGACCAATGGACCGAAGGTAACCCCGGATTGACCAAGGCAACCGAGGCGGCGCGGGCCGTGGGCGGCTTGCTGGC
AGTACCCTTTATTGAGGGGGTACGTCATGAGTAA

Upstream 100 bases:

>100_bases
ACTATGAATTGAAAAAAGTGCTCCCAATTCCACAGAAAGCAATCATGCAGTCTCATGGCGCTTTGCTTGGTGAATGCCGG
ATGTTGAAGCAGGGGACGCT

Downstream 100 bases:

>100_bases
GCCGACCGACCTTAACGACCTTCATCAATTTGCTGGGCCGGAAGCTGTCAAGGCCTGCATTGAAGCAGCAGCACCCGTGA
CGGGCGCCCCGTCGAGCACT

Product: TOPRIM domain-containing protein

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNNPIDGFRQFMRNAGLEPPGEIVANGSLNRFTVPGDKPRSLNGWYVFHADTPAAGSFGCWKRQLNETWSAKEYHSMDPA
EKAAYSAKMETIKRQREEEQVRIHAECRAWCADVWGKAEDATNDHPYLKAKGVKSFGLKCFRGSLLIPIQDIAGNIYGMQ
FIRPDGSKTFKTGTAKRGHFYSMGEGKGNTLCIAEGYATAASIHQATGYPVLTAFDAGNLKPVAENVRAKVPQLNIIICA
DNDQWTEGNPGLTKATEAARAVGGLLAVPFIEGVRHE

Sequences:

>Translated_277_residues
MNNPIDGFRQFMRNAGLEPPGEIVANGSLNRFTVPGDKPRSLNGWYVFHADTPAAGSFGCWKRQLNETWSAKEYHSMDPA
EKAAYSAKMETIKRQREEEQVRIHAECRAWCADVWGKAEDATNDHPYLKAKGVKSFGLKCFRGSLLIPIQDIAGNIYGMQ
FIRPDGSKTFKTGTAKRGHFYSMGEGKGNTLCIAEGYATAASIHQATGYPVLTAFDAGNLKPVAENVRAKVPQLNIIICA
DNDQWTEGNPGLTKATEAARAVGGLLAVPFIEGVRHE
>Mature_277_residues
MNNPIDGFRQFMRNAGLEPPGEIVANGSLNRFTVPGDKPRSLNGWYVFHADTPAAGSFGCWKRQLNETWSAKEYHSMDPA
EKAAYSAKMETIKRQREEEQVRIHAECRAWCADVWGKAEDATNDHPYLKAKGVKSFGLKCFRGSLLIPIQDIAGNIYGMQ
FIRPDGSKTFKTGTAKRGHFYSMGEGKGNTLCIAEGYATAASIHQATGYPVLTAFDAGNLKPVAENVRAKVPQLNIIICA
DNDQWTEGNPGLTKATEAARAVGGLLAVPFIEGVRHE

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4643

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 30351; Mature: 30351

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPIDGFRQFMRNAGLEPPGEIVANGSLNRFTVPGDKPRSLNGWYVFHADTPAAGSFGC
CCCHHHHHHHHHHHCCCCCCHHEEECCCCCEEECCCCCCCCCCCEEEEECCCCCCCCHHH
WKRQLNETWSAKEYHSMDPAEKAAYSAKMETIKRQREEEQVRIHAECRAWCADVWGKAED
HHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCC
ATNDHPYLKAKGVKSFGLKCFRGSLLIPIQDIAGNIYGMQFIRPDGSKTFKTGTAKRGHF
CCCCCCEEEECCCHHHCHHHHCCCEEEEHHHHCCCEEEEEEECCCCCCEEECCCCCCCCE
YSMGEGKGNTLCIAEGYATAASIHQATGYPVLTAFDAGNLKPVAENVRAKVPQLNIIICA
EEECCCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEE
DNDQWTEGNPGLTKATEAARAVGGLLAVPFIEGVRHE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNPIDGFRQFMRNAGLEPPGEIVANGSLNRFTVPGDKPRSLNGWYVFHADTPAAGSFGC
CCCHHHHHHHHHHHCCCCCCHHEEECCCCCEEECCCCCCCCCCCEEEEECCCCCCCCHHH
WKRQLNETWSAKEYHSMDPAEKAAYSAKMETIKRQREEEQVRIHAECRAWCADVWGKAED
HHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCC
ATNDHPYLKAKGVKSFGLKCFRGSLLIPIQDIAGNIYGMQFIRPDGSKTFKTGTAKRGHF
CCCCCCEEEECCCHHHCHHHHCCCEEEEHHHHCCCEEEEEEECCCCCCEEECCCCCCCCE
YSMGEGKGNTLCIAEGYATAASIHQATGYPVLTAFDAGNLKPVAENVRAKVPQLNIIICA
EEECCCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEE
DNDQWTEGNPGLTKATEAARAVGGLLAVPFIEGVRHE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]