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The map label for this gene is bioH [H]

Identifier: 118580508

GI number: 118580508

Start: 2251112

End: 2251939

Strand: Reverse

Name: bioH [H]

Synonym: Ppro_2092

Alternate gene names: 118580508

Gene position: 2251939-2251112 (Counterclockwise)

Preceding gene: 118580509

Following gene: 118580507

Centisome position: 56.19

GC content: 65.58

Gene sequence:

>828_bases
ATGCCTTGGCTCAGGCTCGGGAGCGGACGGCGCCTCTGGTACGAGGATCAGGGGGAGGGGATTCCGCTGGTGCTGCTGCA
CGGCTGGTGCATGTCGTCGCAGGTGTGGCGCTTCCAGCTGGAGACCCTTCAGCGTGATTTTCGCCTGATCGCGCCCGACC
TGGCCGGCCATGGGCGATCCGACCTCTCCCCCGACGGCTTCACTCTTTCCGCCCTGTGCGCTGATCTGGATGAGCTGTTC
CGACATCTGGATCTCAGGAGCGCGCTCCTGGCGGGGTGGTCCCTGGGGGCGCAGCTGGCCCTGCAGGCCTGGGGAACGCT
GCGGGAGCGCTTGTCCGGGCTGGTGCTGGTTTCCGGTACCCCCCGTTTCATTGCGGACGACGACTTCCCCCATGCCCTGG
AGGCCAGGGAACTGCGCGGCATGCAGGCCAGGATGCGCCGCAACCCCTTAATCACGCTGCGGGATTTTGTCTCGCGCATG
TTCGCCGAGGCCGAGCGCGATGTCTGCTTCCCTGACGGACGTATCCAGCGAATGCTGGCTTCGATCCCGCTGCCGGAGGG
GGAGGTGGCGCTGCAGGGGCTCAGGCTTCTCGGGGAGACCGACCTGCGCTCCCTGCTCCCGGCCGTGGATCTGCCGACAC
TGATCATGGGCGGGGACCGGGACGCCATCTGCCTGCCCCTGGCTTCAGACTACATGGCGCGCAGGATTCCGGAATGCCGG
CAGGTCGTTTTCGCCGGCTGCGGACATGCGCCCTTCTTGACCCGCTGCACTGAATTCAACGATGCAATCACGCGTTTCAG
CAGGAGGATTTTTGAACAGGGTAGATGA

Upstream 100 bases:

>100_bases
GCCGGTTGCGCTGCACGGTCATGGCAACCCATTCGCCCCAGGACCTGACCTGGGCCGCGGAACGGATCACCCATATCGGC
AGGCGTCTGGGAGTGGTTTG

Downstream 100 bases:

>100_bases
CAGAACGAGGATCGGCAGGGGATTCCACCGCCAGGCCGGCGAGTATGACCGACATGCGGTGGTGCAGAAGCGGGTGGTGA
CCAACCTGACTCGTCTGGTG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPWLRLGSGRRLWYEDQGEGIPLVLLHGWCMSSQVWRFQLETLQRDFRLIAPDLAGHGRSDLSPDGFTLSALCADLDELF
RHLDLRSALLAGWSLGAQLALQAWGTLRERLSGLVLVSGTPRFIADDDFPHALEARELRGMQARMRRNPLITLRDFVSRM
FAEAERDVCFPDGRIQRMLASIPLPEGEVALQGLRLLGETDLRSLLPAVDLPTLIMGGDRDAICLPLASDYMARRIPECR
QVVFAGCGHAPFLTRCTEFNDAITRFSRRIFEQGR

Sequences:

>Translated_275_residues
MPWLRLGSGRRLWYEDQGEGIPLVLLHGWCMSSQVWRFQLETLQRDFRLIAPDLAGHGRSDLSPDGFTLSALCADLDELF
RHLDLRSALLAGWSLGAQLALQAWGTLRERLSGLVLVSGTPRFIADDDFPHALEARELRGMQARMRRNPLITLRDFVSRM
FAEAERDVCFPDGRIQRMLASIPLPEGEVALQGLRLLGETDLRSLLPAVDLPTLIMGGDRDAICLPLASDYMARRIPECR
QVVFAGCGHAPFLTRCTEFNDAITRFSRRIFEQGR
>Mature_274_residues
PWLRLGSGRRLWYEDQGEGIPLVLLHGWCMSSQVWRFQLETLQRDFRLIAPDLAGHGRSDLSPDGFTLSALCADLDELFR
HLDLRSALLAGWSLGAQLALQAWGTLRERLSGLVLVSGTPRFIADDDFPHALEARELRGMQARMRRNPLITLRDFVSRMF
AEAERDVCFPDGRIQRMLASIPLPEGEVALQGLRLLGETDLRSLLPAVDLPTLIMGGDRDAICLPLASDYMARRIPECRQ
VVFAGCGHAPFLTRCTEFNDAITRFSRRIFEQGR

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=265, Percent_Identity=31.6981132075472, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 31017; Mature: 30886

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWLRLGSGRRLWYEDQGEGIPLVLLHGWCMSSQVWRFQLETLQRDFRLIAPDLAGHGRS
CCCEECCCCCEEEECCCCCCCCEEEEECCHHCCHHHHHHHHHHHHHHHEECCHHCCCCCC
DLSPDGFTLSALCADLDELFRHLDLRSALLAGWSLGAQLALQAWGTLRERLSGLVLVSGT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
PRFIADDDFPHALEARELRGMQARMRRNPLITLRDFVSRMFAEAERDVCFPDGRIQRMLA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHH
SIPLPEGEVALQGLRLLGETDLRSLLPAVDLPTLIMGGDRDAICLPLASDYMARRIPECR
HCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCEEECCCCCEEEEECHHHHHHHHCHHHH
QVVFAGCGHAPFLTRCTEFNDAITRFSRRIFEQGR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PWLRLGSGRRLWYEDQGEGIPLVLLHGWCMSSQVWRFQLETLQRDFRLIAPDLAGHGRS
CCEECCCCCEEEECCCCCCCCEEEEECCHHCCHHHHHHHHHHHHHHHEECCHHCCCCCC
DLSPDGFTLSALCADLDELFRHLDLRSALLAGWSLGAQLALQAWGTLRERLSGLVLVSGT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
PRFIADDDFPHALEARELRGMQARMRRNPLITLRDFVSRMFAEAERDVCFPDGRIQRMLA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHH
SIPLPEGEVALQGLRLLGETDLRSLLPAVDLPTLIMGGDRDAICLPLASDYMARRIPECR
HCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCEEECCCCCEEEEECHHHHHHHHCHHHH
QVVFAGCGHAPFLTRCTEFNDAITRFSRRIFEQGR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA