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The map label for this gene is leuD [H]

Identifier: 118580344

GI number: 118580344

Start: 2077213

End: 2077740

Strand: Direct

Name: leuD [H]

Synonym: Ppro_1927

Alternate gene names: 118580344

Gene position: 2077213-2077740 (Clockwise)

Preceding gene: 118580343

Following gene: 118580349

Centisome position: 51.83

GC content: 61.17

Gene sequence:

>528_bases
ATGAAAACCTTTGGAGGCCCTGCCCTCTTTCTCGACCGTTCCGACATCAACACCGACGAGATCATCCCGGCCAAATATCT
GACCGAGATAACCAAGGAAGCGCTCAAGCCCTACATCCTGGAGGACCTGAAGCTGGCCGGCTTCGACCCCAAGGGGGAGA
CGCTGAAACAGGCACGGGTGATCGTCTCCCGGGCCAACTTCGGCTGCGGCTCCTCCCGCGAGCATGCCCCCTGGGTGTTC
GAGGTGAACGGCATCAACACCGTGATCGCCCAGTCCTTCGCCCGCATCTTCCGCCAGAACATGTTCAACTGCGGCATGCT
GGCCATCGAATTGCCCCAGGCCGACCTGGAGCGGCTGTTCTCCCTGGCTGGCAACGACGATGCCCGGATCTGCATCGACA
TCGAGGCCCAGAGCCTGGCCCTGGACGCAGGCGGAAAGACCGAGGAGTTCGTTTTCCAACTCTCTCCCTTCGACAAGGCG
CTGGTCATGGCCGGCGGCTGGGTTGATTATGCGGACAGGAACTACTGA

Upstream 100 bases:

>100_bases
CATGTGGCCGCCCTTGCCCCCTCCCCCTGCTAACAAATACAGCAGGGCACCCACACGGGGGTGCCCCTACGCCACAATTC
ATCCGCCAAGGAGTGAACCT

Downstream 100 bases:

>100_bases
TTCATGACCGGATCATGACCAGACGCACAAAAGCCCCGCCTTGGGCGGGGCTTTTGTGCGTCTGGTGCTCTGCTCGAACC
GGTCCCCTGTCCTGGGAATG

Product: aconitate hydratase domain-containing protein

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKTFGGPALFLDRSDINTDEIIPAKYLTEITKEALKPYILEDLKLAGFDPKGETLKQARVIVSRANFGCGSSREHAPWVF
EVNGINTVIAQSFARIFRQNMFNCGMLAIELPQADLERLFSLAGNDDARICIDIEAQSLALDAGGKTEEFVFQLSPFDKA
LVMAGGWVDYADRNY

Sequences:

>Translated_175_residues
MKTFGGPALFLDRSDINTDEIIPAKYLTEITKEALKPYILEDLKLAGFDPKGETLKQARVIVSRANFGCGSSREHAPWVF
EVNGINTVIAQSFARIFRQNMFNCGMLAIELPQADLERLFSLAGNDDARICIDIEAQSLALDAGGKTEEFVFQLSPFDKA
LVMAGGWVDYADRNY
>Mature_175_residues
MKTFGGPALFLDRSDINTDEIIPAKYLTEITKEALKPYILEDLKLAGFDPKGETLKQARVIVSRANFGCGSSREHAPWVF
EVNGINTVIAQSFARIFRQNMFNCGMLAIELPQADLERLFSLAGNDDARICIDIEAQSLALDAGGKTEEFVFQLSPFDKA
LVMAGGWVDYADRNY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=167, Percent_Identity=35.3293413173653, Blast_Score=100, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=176, Percent_Identity=33.5227272727273, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320440, Length=113, Percent_Identity=36.283185840708, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 19431; Mature: 19431

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFGGPALFLDRSDINTDEIIPAKYLTEITKEALKPYILEDLKLAGFDPKGETLKQARV
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHH
IVSRANFGCGSSREHAPWVFEVNGINTVIAQSFARIFRQNMFNCGMLAIELPQADLERLF
HHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHH
SLAGNDDARICIDIEAQSLALDAGGKTEEFVFQLSPFDKALVMAGGWVDYADRNY
HHCCCCCCEEEEEECCCEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKTFGGPALFLDRSDINTDEIIPAKYLTEITKEALKPYILEDLKLAGFDPKGETLKQARV
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHH
IVSRANFGCGSSREHAPWVFEVNGINTVIAQSFARIFRQNMFNCGMLAIELPQADLERLF
HHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHH
SLAGNDDARICIDIEAQSLALDAGGKTEEFVFQLSPFDKALVMAGGWVDYADRNY
HHCCCCCCEEEEEECCCEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA