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The map label for this gene is dosC [H]

Identifier: 118580254

GI number: 118580254

Start: 1974376

End: 1975401

Strand: Direct

Name: dosC [H]

Synonym: Ppro_1834

Alternate gene names: 118580254

Gene position: 1974376-1975401 (Clockwise)

Preceding gene: 118580253

Following gene: 118580256

Centisome position: 49.26

GC content: 55.07

Gene sequence:

>1026_bases
ATGACAAATGCCCCTCGGCTTCTGGACAAGCTCGTCAGCATGACGGCCATACACGATGTGGAGCTCATGGAATTCAGCCT
GCTGAAGACCCTGGTGGAATTTGTGCGACCGCAGGAACTGATGATCATCAAACTGGACAGGAACGGGCACCCCTGCTACC
AGCTGAGCCTGAAGCATGAGCAGTATGAGATCATTCACGACGACATCTGCATGCCGGACGAGATCATGGCGGCAATCGAA
CTCGTACGGAGGACGAAACAACCCTTCAACCGGCGGCTCGATCCCCTCAGGCTACTCGCCGTCTGGAACATTCTGCAGAC
AAAATCGCAGGATGTTTTTCTCGTGACCATAACCGCCAACGAAATAGGCAAACTGGACGCCTACATGATAAACGGCCTTC
TGGGGGTCTACCGCAATTTCTTTGCCGTCCTCCACGAGGCTCAACGTGACCAGTTGACCGGCCTGGCCAACAGGAAGACC
TTCGACGAGGTTGTCAACAAGATTTACTTCCAGCTTCCCCCTTCCACGGATCCTGTCCCGGTGGAACGACGGGCGGACAG
CCCATCCCGCAGCAGGGGTTTCTGGGTGGGAATGGCGGATCTGGACAATTTCAAGCGGATCAACGACTCTTGGGGGCATC
TGTACGGAGACGAGGTGCTCCTGCTGATATCACAACTCATGCAGGGTCACTTCAGGGAAAGCGACTACCTGTTCCGTTTT
GGAGGTGAGGAGTTCGTTATCATCATCCGCGCGGCCCACGAAGATCAGGCCGAACAGGCCTTTGAACGCTTCCGCAGGGC
GGTGGAGGAACACATCTTTCCCCAGATCGGGCAGGTGACCATCAGCATCGGGGTTGCGAAAATGGACGCCAGCATTTTTA
CGGCAACCCTGCTCGATCATGCCGACAAGGCACTGTACCATGCAAAGAAGAACGGTCGTAACCAAGTCTGTTTTTTCGAA
AAGCTGCTCAGAGACGGACTGGTTGAGGAGTCAGACATCAGGACGGGAGAGGTGGAACTCTTCTGA

Upstream 100 bases:

>100_bases
AGTCTCTGACCCGCTGGCCGCGCGCATTCCCGTATTGCGCTTGATGGGCATCTTACTAAAAATTAATCAAAACTATCCCG
TCAGCGTCAGGAAACTTCCT

Downstream 100 bases:

>100_bases
TACTGGTCATCCGGAGAATGTTATAGCTCCAGATGGTTGTCAAGATCGGCCTTGAGCGCCCTGAACAGGTCGCTCTTCTC
ATAGGTTTCGAAGAACAGGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTNAPRLLDKLVSMTAIHDVELMEFSLLKTLVEFVRPQELMIIKLDRNGHPCYQLSLKHEQYEIIHDDICMPDEIMAAIE
LVRRTKQPFNRRLDPLRLLAVWNILQTKSQDVFLVTITANEIGKLDAYMINGLLGVYRNFFAVLHEAQRDQLTGLANRKT
FDEVVNKIYFQLPPSTDPVPVERRADSPSRSRGFWVGMADLDNFKRINDSWGHLYGDEVLLLISQLMQGHFRESDYLFRF
GGEEFVIIIRAAHEDQAEQAFERFRRAVEEHIFPQIGQVTISIGVAKMDASIFTATLLDHADKALYHAKKNGRNQVCFFE
KLLRDGLVEESDIRTGEVELF

Sequences:

>Translated_341_residues
MTNAPRLLDKLVSMTAIHDVELMEFSLLKTLVEFVRPQELMIIKLDRNGHPCYQLSLKHEQYEIIHDDICMPDEIMAAIE
LVRRTKQPFNRRLDPLRLLAVWNILQTKSQDVFLVTITANEIGKLDAYMINGLLGVYRNFFAVLHEAQRDQLTGLANRKT
FDEVVNKIYFQLPPSTDPVPVERRADSPSRSRGFWVGMADLDNFKRINDSWGHLYGDEVLLLISQLMQGHFRESDYLFRF
GGEEFVIIIRAAHEDQAEQAFERFRRAVEEHIFPQIGQVTISIGVAKMDASIFTATLLDHADKALYHAKKNGRNQVCFFE
KLLRDGLVEESDIRTGEVELF
>Mature_340_residues
TNAPRLLDKLVSMTAIHDVELMEFSLLKTLVEFVRPQELMIIKLDRNGHPCYQLSLKHEQYEIIHDDICMPDEIMAAIEL
VRRTKQPFNRRLDPLRLLAVWNILQTKSQDVFLVTITANEIGKLDAYMINGLLGVYRNFFAVLHEAQRDQLTGLANRKTF
DEVVNKIYFQLPPSTDPVPVERRADSPSRSRGFWVGMADLDNFKRINDSWGHLYGDEVLLLISQLMQGHFRESDYLFRFG
GEEFVIIIRAAHEDQAEQAFERFRRAVEEHIFPQIGQVTISIGVAKMDASIFTATLLDHADKALYHAKKNGRNQVCFFEK
LLRDGLVEESDIRTGEVELF

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=33.879781420765, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=33.7209302325581, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787262, Length=178, Percent_Identity=33.1460674157303, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786584, Length=119, Percent_Identity=40.3361344537815, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787816, Length=178, Percent_Identity=29.2134831460674, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787056, Length=182, Percent_Identity=29.6703296703297, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788381, Length=227, Percent_Identity=29.5154185022026, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=28.8135593220339, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787802, Length=139, Percent_Identity=34.5323741007194, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788085, Length=178, Percent_Identity=33.1460674157303, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87082007, Length=130, Percent_Identity=37.6923076923077, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=184, Percent_Identity=30.9782608695652, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39397; Mature: 39266

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAPRLLDKLVSMTAIHDVELMEFSLLKTLVEFVRPQELMIIKLDRNGHPCYQLSLKHE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCC
QYEIIHDDICMPDEIMAAIELVRRTKQPFNRRLDPLRLLAVWNILQTKSQDVFLVTITAN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECC
EIGKLDAYMINGLLGVYRNFFAVLHEAQRDQLTGLANRKTFDEVVNKIYFQLPPSTDPVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCCC
VERRADSPSRSRGFWVGMADLDNFKRINDSWGHLYGDEVLLLISQLMQGHFRESDYLFRF
CCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEE
GGEEFVIIIRAAHEDQAEQAFERFRRAVEEHIFPQIGQVTISIGVAKMDASIFTATLLDH
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHH
ADKALYHAKKNGRNQVCFFEKLLRDGLVEESDIRTGEVELF
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEC
>Mature Secondary Structure 
TNAPRLLDKLVSMTAIHDVELMEFSLLKTLVEFVRPQELMIIKLDRNGHPCYQLSLKHE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCC
QYEIIHDDICMPDEIMAAIELVRRTKQPFNRRLDPLRLLAVWNILQTKSQDVFLVTITAN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECC
EIGKLDAYMINGLLGVYRNFFAVLHEAQRDQLTGLANRKTFDEVVNKIYFQLPPSTDPVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCCC
VERRADSPSRSRGFWVGMADLDNFKRINDSWGHLYGDEVLLLISQLMQGHFRESDYLFRF
CCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEE
GGEEFVIIIRAAHEDQAEQAFERFRRAVEEHIFPQIGQVTISIGVAKMDASIFTATLLDH
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHH
ADKALYHAKKNGRNQVCFFEKLLRDGLVEESDIRTGEVELF
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]