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The map label for this gene is thiF [H]

Identifier: 118580253

GI number: 118580253

Start: 1973449

End: 1974252

Strand: Direct

Name: thiF [H]

Synonym: Ppro_1833

Alternate gene names: 118580253

Gene position: 1973449-1974252 (Clockwise)

Preceding gene: 118580252

Following gene: 118580254

Centisome position: 49.24

GC content: 63.06

Gene sequence:

>804_bases
TTGATACAGATACGGCTGAACGAAAAAACCATCATGGTGGACGACGGGCTCACCCTGGCAATGCTGGCCAAGCAGCGCCG
ACCGGGTGCCGACGTCCTGATCCTGAACGGCTTTCCTGCCGAGGATGATACGCAGATCAACGATGGGGATGCGGTATTCC
TGATCAAACGGGGTGAGCAGCCGAACGAGGAAGAGCTGGAGTGGCTGATGGCGTCCCGTCACACCCCCGGCGTGCATGGA
CGCCTGAAGGGTGCCTGCGTGGGGATCGCGGGAGTGGGCGGGCTGGGTTCGGCTGTGGCGGTGGCCCTGGCGCGGGTGGG
TGTTGGCAGGCTGGTTATCGCCGACTTCGACCTGGTGGAGCCATCCAACCTGAACCGTCAGCAGTACTTCATAGACCAAC
TTGGTATGTTCAAGGTGGATGCCCTGGATGACAACCTGCGCCGCATCAACCCGTATGTCAGGGTGGAGCGCCACCAGCTG
TTGCTCACCCCGGAGAACATACCCGTCATCTTCGCCCCCTGTACCATCGTGGTGGAGGCTTTTGACCGGGCCGACATGAA
GGCCATGCTGGTGGACAGCCTGTTAAGCTCCCTGGCCGGCGTCACCGTGGTGGCAGCCTCGGGGGTGGCCGGTTATGGCT
CCAACAACGATATAACCACCAGACGGGTTTCCCGAAGGCTCTACCTGGTGGGCGACTCAGTATCCGAGGCCGGTCCGGGG
AGCGGCCTGATGGCCCCGCGGGTCGCCATCGCCGCCGGACACCAGGCCAACCAGGTGGTGCGGATCATCCTGGGGGAGGA
GTAG

Upstream 100 bases:

>100_bases
TCTAGGGTATTCGCTGTCGGCCATGGCGAGAGGTGATCCAGAAGCAGTAACACGCGAAGTAACAGCAGATATCTAGTTAA
AAAACAGGAATTTTGAGAAA

Downstream 100 bases:

>100_bases
GTTCCACACCATCACTTCTGCGCAGTCTCTGACCCGCTGGCCGCGCGCATTCCCGTATTGCGCTTGATGGGCATCTTACT
AAAAATTAATCAAAACTATC

Product: thiamine biosynthesis protein ThiF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MIQIRLNEKTIMVDDGLTLAMLAKQRRPGADVLILNGFPAEDDTQINDGDAVFLIKRGEQPNEEELEWLMASRHTPGVHG
RLKGACVGIAGVGGLGSAVAVALARVGVGRLVIADFDLVEPSNLNRQQYFIDQLGMFKVDALDDNLRRINPYVRVERHQL
LLTPENIPVIFAPCTIVVEAFDRADMKAMLVDSLLSSLAGVTVVAASGVAGYGSNNDITTRRVSRRLYLVGDSVSEAGPG
SGLMAPRVAIAAGHQANQVVRIILGEE

Sequences:

>Translated_267_residues
MIQIRLNEKTIMVDDGLTLAMLAKQRRPGADVLILNGFPAEDDTQINDGDAVFLIKRGEQPNEEELEWLMASRHTPGVHG
RLKGACVGIAGVGGLGSAVAVALARVGVGRLVIADFDLVEPSNLNRQQYFIDQLGMFKVDALDDNLRRINPYVRVERHQL
LLTPENIPVIFAPCTIVVEAFDRADMKAMLVDSLLSSLAGVTVVAASGVAGYGSNNDITTRRVSRRLYLVGDSVSEAGPG
SGLMAPRVAIAAGHQANQVVRIILGEE
>Mature_267_residues
MIQIRLNEKTIMVDDGLTLAMLAKQRRPGADVLILNGFPAEDDTQINDGDAVFLIKRGEQPNEEELEWLMASRHTPGVHG
RLKGACVGIAGVGGLGSAVAVALARVGVGRLVIADFDLVEPSNLNRQQYFIDQLGMFKVDALDDNLRRINPYVRVERHQL
LLTPENIPVIFAPCTIVVEAFDRADMKAMLVDSLLSSLAGVTVVAASGVAGYGSNNDITTRRVSRRLYLVGDSVSEAGPG
SGLMAPRVAIAAGHQANQVVRIILGEE

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=136, Percent_Identity=38.2352941176471, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787048, Length=189, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789177, Length=107, Percent_Identity=34.5794392523364, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=197, Percent_Identity=31.4720812182741, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=205, Percent_Identity=28.780487804878, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582879, Length=195, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 28691; Mature: 28691

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIRLNEKTIMVDDGLTLAMLAKQRRPGADVLILNGFPAEDDTQINDGDAVFLIKRGEQ
CEEEEECCEEEEEECCCEEEEHHHCCCCCCCEEEECCCCCCCCCEECCCCEEEEEECCCC
PNEEELEWLMASRHTPGVHGRLKGACVGIAGVGGLGSAVAVALARVGVGRLVIADFDLVE
CCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCC
PSNLNRQQYFIDQLGMFKVDALDDNLRRINPYVRVERHQLLLTPENIPVIFAPCTIVVEA
CCCCCHHHHHHHHCCCEEEECCCCCHHHCCCEEEEECEEEEECCCCCCEEEECHHHEEEH
FDRADMKAMLVDSLLSSLAGVTVVAASGVAGYGSNNDITTRRVSRRLYLVGDSVSEAGPG
HCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHCEEEEEEECCCCCCCCC
SGLMAPRVAIAAGHQANQVVRIILGEE
CCCCCCEEEEECCCCCCEEEEEEECCC
>Mature Secondary Structure
MIQIRLNEKTIMVDDGLTLAMLAKQRRPGADVLILNGFPAEDDTQINDGDAVFLIKRGEQ
CEEEEECCEEEEEECCCEEEEHHHCCCCCCCEEEECCCCCCCCCEECCCCEEEEEECCCC
PNEEELEWLMASRHTPGVHGRLKGACVGIAGVGGLGSAVAVALARVGVGRLVIADFDLVE
CCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCC
PSNLNRQQYFIDQLGMFKVDALDDNLRRINPYVRVERHQLLLTPENIPVIFAPCTIVVEA
CCCCCHHHHHHHHCCCEEEECCCCCHHHCCCEEEEECEEEEECCCCCCEEEECHHHEEEH
FDRADMKAMLVDSLLSSLAGVTVVAASGVAGYGSNNDITTRRVSRRLYLVGDSVSEAGPG
HCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHCEEEEEEECCCCCCCCC
SGLMAPRVAIAAGHQANQVVRIILGEE
CCCCCCEEEEECCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]