Click here to switch to the map view.

The map label for this gene is proS

Identifier: 118580173

GI number: 118580173

Start: 1884697

End: 1886427

Strand: Direct

Name: proS

Synonym: Ppro_1753

Alternate gene names: 118580173

Gene position: 1884697-1886427 (Clockwise)

Preceding gene: 118580171

Following gene: 118580174

Centisome position: 47.02

GC content: 60.77

Gene sequence:

>1731_bases
ATGTTTTATTCGCGTTATTTCATCCCAACTATCAAGGAGACCCCCTCCGACGCCGAGGTTGTTTCCCATCAACTGATGCT
GCGCGCCGGCATGATCCGTAAACTTGCGGCCGGGATCTACAACTACCTGCCGCTGGGGCTGCGCTCCATCCGTAAGTTCG
AGGCGATCGTCCGCGAGGAGATGAACAGGGCAGGGGCCATCGAGATGCTCATGCCCTCCGTGCAGCCGGCAGAACTGTGG
GAAGAATCGGGGCGCTGGTCCTTCTACGGCAAGGAACTGCTGCGCTTCAGGGACCGCAAGGATGGGGAGTTCTGCATGGG
GCCGACCCATGAAGAGGTTATTACCGATATGGTCCGGCGGGAGATTAAGAGCTACCGCCAGATGCCGGTCAACTTCTACC
AGATCCAGACCAAGTTTCGCGACGAAATCCGCCCTCGCTTCGGCCTGATGCGCGGCCGGGAATTCATCATGAAGGACGCC
TACTCCTTTGACGTGGATAGCGCGGCAGCGGACGGCTCCTATGAGCTGATGTTCCAGGCCTACATGCGCATCTTCGAGCG
CTGCGGCCTGAACTTCCGCGCGGTGGAGGCTGATACCGGCACCATCGGCGGTTCCTCATCCCATGAATTCATGGTCCTGG
CCGACTCCGGCGAGGATGCCATCGTCTCCTGCGATTCCTGCCGTTACGCCGCCAATGTGGAAAAGGCGGAGTCACGTCCC
GCCGCCGGCGCCAGCACTGAGGAACAGCTGGAGCTAACGAAAACCGCCACACCGGACATGAAGAGCATCGCCGATGTGGC
CGCCTTCCTGGGGCTGGCAACGGACAGGACCATCAAGGCGCTGGTCTACTCCTCCACCACCGGCGAGCATGTCATGGCCA
TCCTGCGCGGTGACCATGAACTGAACGAGATCAAGCTCAAGAACTGTCTTGGGTGGGACGAAATCCAGATGGCCACCGAC
GAGGAGATACTGGCTTACACCGGCTCTCCGGTCGGCTTCCTCGGGCCAATGGGGCTGAAGCAGGGGGTCGTGGTCGTTGC
CGACCTGGCGCTGCGAGGCATGGCCAATGCCGTGATCGGCGCCAATGAAAAGGACATGCACTACATAAATGCCAACCTGG
GGCGCGATTTTACGTCGGACCGCTTCGTCGACCTGCGAAACGTGGAGGCCGGCGATCCCTGCCCGCGCTGCGAGGGGGGC
AAACTGGAGATGTGGCGTGGCATCGAGGTGGGGCATGTCTTCAAGCTGGGAACCAAGTACTCCCAGGCCCTGGGAGCCAC
CTACCTGGACGCCGACGGCAAGGAGCAGGTCATCTTCATGGGCTGTTACGGCATCGGTATCGGCCGTACCGTTGCCGCCG
CCATCGAACAGAACCACGATGACAACGGCATCATTTTCCCGCTGCCGCTGGCGCCGTTCCATTGTTCGGTGGTGGCTCTG
AACACCAAGGACAAGGGTGTCATGGCAGCCGCCGAGGAGATGTACTTCAGGTTGGAGCAGCAGGGGATAGAGGTGCTCTT
CGATGACCGGGACGAGCGCCCCGGCGTCAAGTTCAAGGACAACGACCTGATCGGCATTCCGCTGCGTATCGTGGTGGGCA
GCAAGGGACTGTCCGAAGGCAAGGTTGAGGTCAAGATTCGCGCAAGCGGTGAGATGCTCCTGCTCTCTCCGGACGAAGCC
GTGGAGACCATCGTCCGCATGGTCAGGGATGCCGTGACAAGCGCCCAATAA

Upstream 100 bases:

>100_bases
GAGGATCAGTTCAACCGCTGCTCCGGCGCGGCCGGGTCTCTGCTCCTGTTTCCCGGAAAAACGCCGTAAGCAGTACTACT
TAACCATACAAGGGGTTCAG

Downstream 100 bases:

>100_bases
AGCTACAATTTCGAGGTTGTGATGCAGATAAGTATTTGGGACCCCCAGCACGAATCCATGCCGCGGGAGGAACTGGAACA
GCTTCAACTGGAACGACTCC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MFYSRYFIPTIKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKFEAIVREEMNRAGAIEMLMPSVQPAELW
EESGRWSFYGKELLRFRDRKDGEFCMGPTHEEVITDMVRREIKSYRQMPVNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
YSFDVDSAAADGSYELMFQAYMRIFERCGLNFRAVEADTGTIGGSSSHEFMVLADSGEDAIVSCDSCRYAANVEKAESRP
AAGASTEEQLELTKTATPDMKSIADVAAFLGLATDRTIKALVYSSTTGEHVMAILRGDHELNEIKLKNCLGWDEIQMATD
EEILAYTGSPVGFLGPMGLKQGVVVVADLALRGMANAVIGANEKDMHYINANLGRDFTSDRFVDLRNVEAGDPCPRCEGG
KLEMWRGIEVGHVFKLGTKYSQALGATYLDADGKEQVIFMGCYGIGIGRTVAAAIEQNHDDNGIIFPLPLAPFHCSVVAL
NTKDKGVMAAAEEMYFRLEQQGIEVLFDDRDERPGVKFKDNDLIGIPLRIVVGSKGLSEGKVEVKIRASGEMLLLSPDEA
VETIVRMVRDAVTSAQ

Sequences:

>Translated_576_residues
MFYSRYFIPTIKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKFEAIVREEMNRAGAIEMLMPSVQPAELW
EESGRWSFYGKELLRFRDRKDGEFCMGPTHEEVITDMVRREIKSYRQMPVNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
YSFDVDSAAADGSYELMFQAYMRIFERCGLNFRAVEADTGTIGGSSSHEFMVLADSGEDAIVSCDSCRYAANVEKAESRP
AAGASTEEQLELTKTATPDMKSIADVAAFLGLATDRTIKALVYSSTTGEHVMAILRGDHELNEIKLKNCLGWDEIQMATD
EEILAYTGSPVGFLGPMGLKQGVVVVADLALRGMANAVIGANEKDMHYINANLGRDFTSDRFVDLRNVEAGDPCPRCEGG
KLEMWRGIEVGHVFKLGTKYSQALGATYLDADGKEQVIFMGCYGIGIGRTVAAAIEQNHDDNGIIFPLPLAPFHCSVVAL
NTKDKGVMAAAEEMYFRLEQQGIEVLFDDRDERPGVKFKDNDLIGIPLRIVVGSKGLSEGKVEVKIRASGEMLLLSPDEA
VETIVRMVRDAVTSAQ
>Mature_576_residues
MFYSRYFIPTIKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKFEAIVREEMNRAGAIEMLMPSVQPAELW
EESGRWSFYGKELLRFRDRKDGEFCMGPTHEEVITDMVRREIKSYRQMPVNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
YSFDVDSAAADGSYELMFQAYMRIFERCGLNFRAVEADTGTIGGSSSHEFMVLADSGEDAIVSCDSCRYAANVEKAESRP
AAGASTEEQLELTKTATPDMKSIADVAAFLGLATDRTIKALVYSSTTGEHVMAILRGDHELNEIKLKNCLGWDEIQMATD
EEILAYTGSPVGFLGPMGLKQGVVVVADLALRGMANAVIGANEKDMHYINANLGRDFTSDRFVDLRNVEAGDPCPRCEGG
KLEMWRGIEVGHVFKLGTKYSQALGATYLDADGKEQVIFMGCYGIGIGRTVAAAIEQNHDDNGIIFPLPLAPFHCSVVAL
NTKDKGVMAAAEEMYFRLEQQGIEVLFDDRDERPGVKFKDNDLIGIPLRIVVGSKGLSEGKVEVKIRASGEMLLLSPDEA
VETIVRMVRDAVTSAQ

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=46.4601769911504, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1786392, Length=578, Percent_Identity=51.5570934256055, Blast_Score=590, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI115532348, Length=232, Percent_Identity=37.9310344827586, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI193203271, Length=106, Percent_Identity=33.9622641509434, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=538, Percent_Identity=31.0408921933086, Blast_Score=259, Evalue=6e-70,
Organism=Drosophila melanogaster, GI24656200, Length=332, Percent_Identity=34.9397590361446, Blast_Score=186, Evalue=5e-47,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PELPD (A1APU5)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901423.1
- ProteinModelPortal:   A1APU5
- SMR:   A1APU5
- STRING:   A1APU5
- GeneID:   4572579
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1753
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A1APU5
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64032; Mature: 64032

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYSRYFIPTIKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKFEAIVREE
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIEMLMPSVQPAELWEESGRWSFYGKELLRFRDRKDGEFCMGPTHEEVITDMVRR
HHHCCCCHHHCCCCCHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EIKSYRQMPVNFYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDVDSAAADGSYELMFQA
HHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YMRIFERCGLNFRAVEADTGTIGGSSSHEFMVLADSGEDAIVSCDSCRYAANVEKAESRP
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHCCCC
AAGASTEEQLELTKTATPDMKSIADVAAFLGLATDRTIKALVYSSTTGEHVMAILRGDHE
CCCCCCHHHEEEHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHEEEHHCCCC
LNEIKLKNCLGWDEIQMATDEEILAYTGSPVGFLGPMGLKQGVVVVADLALRGMANAVIG
CCHHHHHHCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCC
ANEKDMHYINANLGRDFTSDRFVDLRNVEAGDPCPRCEGGKLEMWRGIEVGHVFKLGTKY
CCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEECCCHH
SQALGATYLDADGKEQVIFMGCYGIGIGRTVAAAIEQNHDDNGIIFPLPLAPFHCSVVAL
HHHHCCEEECCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEE
NTKDKGVMAAAEEMYFRLEQQGIEVLFDDRDERPGVKFKDNDLIGIPLRIVVGSKGLSEG
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCEEECEEEEEECCCCCCCC
KVEVKIRASGEMLLLSPDEAVETIVRMVRDAVTSAQ
EEEEEEEECCCEEEECCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFYSRYFIPTIKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKFEAIVREE
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIEMLMPSVQPAELWEESGRWSFYGKELLRFRDRKDGEFCMGPTHEEVITDMVRR
HHHCCCCHHHCCCCCHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EIKSYRQMPVNFYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDVDSAAADGSYELMFQA
HHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YMRIFERCGLNFRAVEADTGTIGGSSSHEFMVLADSGEDAIVSCDSCRYAANVEKAESRP
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHCCCC
AAGASTEEQLELTKTATPDMKSIADVAAFLGLATDRTIKALVYSSTTGEHVMAILRGDHE
CCCCCCHHHEEEHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHEEEHHCCCC
LNEIKLKNCLGWDEIQMATDEEILAYTGSPVGFLGPMGLKQGVVVVADLALRGMANAVIG
CCHHHHHHCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCC
ANEKDMHYINANLGRDFTSDRFVDLRNVEAGDPCPRCEGGKLEMWRGIEVGHVFKLGTKY
CCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEECCCHH
SQALGATYLDADGKEQVIFMGCYGIGIGRTVAAAIEQNHDDNGIIFPLPLAPFHCSVVAL
HHHHCCEEECCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEE
NTKDKGVMAAAEEMYFRLEQQGIEVLFDDRDERPGVKFKDNDLIGIPLRIVVGSKGLSEG
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCEEECEEEEEECCCCCCCC
KVEVKIRASGEMLLLSPDEAVETIVRMVRDAVTSAQ
EEEEEEEECCCEEEECCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA