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The map label for this gene is ispG [H]

Identifier: 118580171

GI number: 118580171

Start: 1882551

End: 1883597

Strand: Direct

Name: ispG [H]

Synonym: Ppro_1751

Alternate gene names: 118580171

Gene position: 1882551-1883597 (Clockwise)

Preceding gene: 118580168

Following gene: 118580173

Centisome position: 46.97

GC content: 62.37

Gene sequence:

>1047_bases
ATGAAAAAAACAACCAGACAGATACGAATCGGTTCCGTTGCCATCGGAGGGGATGCGCCCTGCTCGGTGCAGTCCATGTG
CTCCACGGATACCCGCGATGTGGCCGCAACCCTGGCCCAGATCCAGCGGCTGGCAACTGCCGGCTGCGAAATCATCCGCT
GCGCAGTGCCGGACATGGATGCGGCGGTCGCCTTGGGACGCATAAAGCGGGAGAGTCCCATCCCTGTGGTTGCCGACATC
CATTTCGATTACAAGCTGGCCCTGGCCGTGCTGGAAGGGGGCATCGACGCCCTGCGCCTCAACCCGGGCAACATCGGCGA
GAAATGGAAGGTGGCCGAGGTGGTCAAGGCGGCGGCGGAGCGCCAGGTTCCCATCCGCATCGGCGTCAATGCCGGCTCAC
TGGAAAAGGAACTGCTGGCCAAGTATGGTCACCCAACCGCCGAAGCCATGGTGGAGTCGGCCCTGGGGCATGTGTCAATC
CTGGAGGAGCTCGGCTACCGGGAGATCAAGATATCCCTCAAGGCCTCGGATGTGATGAAGACCGTCAGCGCCTACCGTTT
ACTCTCCCGCAAGGTGGACTACCCCCTGCACATCGGCATCACCGAGGCGGGGACCATATTCTCCGGAACGGTCAAGTCAT
CGGTGGGGCTGGGTATTCTGCTGGCTGACGGCATCGGCGATACCCTGCGGGTCTCCCTCACCGGCGACGTGCTGGATGAG
GTCAGGGTGGGGTACGAGATCTTGAAGAGCCTGGGATTGCGCCAGCGCGGGGTGAATTTCGTATCCTGTCCCACCTGTGG
CAGGTGTCAGATCAACCTGGTCAAGGTTGCCGAGGAGGTGGAGCGGCGGCTGCAGGGGGTGGACAAGCGTATCACCGTGG
CTGTCATGGGATGTGTGGTCAACGGTCCGGGTGAGGCCCGGGAGGCGGACGTGGGCATTGCCGGCGGCAAGGGGGAGGGG
CTCCTCTTCCGTCATGGTGAAATAATCCGCAAGGTCCCCGAGGATCGGCTGGCCGATGCGCTGGTGGAAGAGGTGGAAAA
TCTGTAA

Upstream 100 bases:

>100_bases
AGGGACGGCAGATTACGGAAGAGTGCGGCTGGTTTTGCCATGGTTGCGCCGGCTTGTGCTTCTTGACATTTCCTCACCCG
ATTGGCAGTATGCCAATTCT

Downstream 100 bases:

>100_bases
GAAGGCAAACAGGACGCGTCGATAGTTGCTTCCTGACAGGTCGGACCTATCCAGGGACGGGCACCGGTTTCGGCGCCCGT
CCCTGTTTTTTTGCGGCATC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKTTRQIRIGSVAIGGDAPCSVQSMCSTDTRDVAATLAQIQRLATAGCEIIRCAVPDMDAAVALGRIKRESPIPVVADI
HFDYKLALAVLEGGIDALRLNPGNIGEKWKVAEVVKAAAERQVPIRIGVNAGSLEKELLAKYGHPTAEAMVESALGHVSI
LEELGYREIKISLKASDVMKTVSAYRLLSRKVDYPLHIGITEAGTIFSGTVKSSVGLGILLADGIGDTLRVSLTGDVLDE
VRVGYEILKSLGLRQRGVNFVSCPTCGRCQINLVKVAEEVERRLQGVDKRITVAVMGCVVNGPGEAREADVGIAGGKGEG
LLFRHGEIIRKVPEDRLADALVEEVENL

Sequences:

>Translated_348_residues
MKKTTRQIRIGSVAIGGDAPCSVQSMCSTDTRDVAATLAQIQRLATAGCEIIRCAVPDMDAAVALGRIKRESPIPVVADI
HFDYKLALAVLEGGIDALRLNPGNIGEKWKVAEVVKAAAERQVPIRIGVNAGSLEKELLAKYGHPTAEAMVESALGHVSI
LEELGYREIKISLKASDVMKTVSAYRLLSRKVDYPLHIGITEAGTIFSGTVKSSVGLGILLADGIGDTLRVSLTGDVLDE
VRVGYEILKSLGLRQRGVNFVSCPTCGRCQINLVKVAEEVERRLQGVDKRITVAVMGCVVNGPGEAREADVGIAGGKGEG
LLFRHGEIIRKVPEDRLADALVEEVENL
>Mature_348_residues
MKKTTRQIRIGSVAIGGDAPCSVQSMCSTDTRDVAATLAQIQRLATAGCEIIRCAVPDMDAAVALGRIKRESPIPVVADI
HFDYKLALAVLEGGIDALRLNPGNIGEKWKVAEVVKAAAERQVPIRIGVNAGSLEKELLAKYGHPTAEAMVESALGHVSI
LEELGYREIKISLKASDVMKTVSAYRLLSRKVDYPLHIGITEAGTIFSGTVKSSVGLGILLADGIGDTLRVSLTGDVLDE
VRVGYEILKSLGLRQRGVNFVSCPTCGRCQINLVKVAEEVERRLQGVDKRITVAVMGCVVNGPGEAREADVGIAGGKGEG
LLFRHGEIIRKVPEDRLADALVEEVENL

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=346, Percent_Identity=50, Blast_Score=352, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37156; Mature: 37156

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTTRQIRIGSVAIGGDAPCSVQSMCSTDTRDVAATLAQIQRLATAGCEIIRCAVPDMD
CCCCCCEEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
AAVALGRIKRESPIPVVADIHFDYKLALAVLEGGIDALRLNPGNIGEKWKVAEVVKAAAE
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
RQVPIRIGVNAGSLEKELLAKYGHPTAEAMVESALGHVSILEELGYREIKISLKASDVMK
CCCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHH
TVSAYRLLSRKVDYPLHIGITEAGTIFSGTVKSSVGLGILLADGIGDTLRVSLTGDVLDE
HHHHHHHHHHCCCCCEEEEEECCCCEEECCHHHCCCEEEEEECCCCCEEEEEEEHHHHHH
VRVGYEILKSLGLRQRGVNFVSCPTCGRCQINLVKVAEEVERRLQGVDKRITVAVMGCVV
HHHHHHHHHHCCCHHHCCCEEECCCCCCCEEHHHHHHHHHHHHHHCCCHHEEEEEEEEEE
NGPGEAREADVGIAGGKGEGLLFRHGEIIRKVPEDRLADALVEEVENL
CCCCCCCCCCCEEECCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTTRQIRIGSVAIGGDAPCSVQSMCSTDTRDVAATLAQIQRLATAGCEIIRCAVPDMD
CCCCCCEEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
AAVALGRIKRESPIPVVADIHFDYKLALAVLEGGIDALRLNPGNIGEKWKVAEVVKAAAE
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
RQVPIRIGVNAGSLEKELLAKYGHPTAEAMVESALGHVSILEELGYREIKISLKASDVMK
CCCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHH
TVSAYRLLSRKVDYPLHIGITEAGTIFSGTVKSSVGLGILLADGIGDTLRVSLTGDVLDE
HHHHHHHHHHCCCCCEEEEEECCCCEEECCHHHCCCEEEEEECCCCCEEEEEEEHHHHHH
VRVGYEILKSLGLRQRGVNFVSCPTCGRCQINLVKVAEEVERRLQGVDKRITVAVMGCVV
HHHHHHHHHHCCCHHHCCCEEECCCCCCCEEHHHHHHHHHHHHHHCCCHHEEEEEEEEEE
NGPGEAREADVGIAGGKGEGLLFRHGEIIRKVPEDRLADALVEEVENL
CCCCCCCCCCCEEECCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA