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The map label for this gene is yibK [C]

Identifier: 118580121

GI number: 118580121

Start: 1827002

End: 1827475

Strand: Direct

Name: yibK [C]

Synonym: Ppro_1700

Alternate gene names: 118580121

Gene position: 1827002-1827475 (Clockwise)

Preceding gene: 118580117

Following gene: 118580122

Centisome position: 45.58

GC content: 62.87

Gene sequence:

>474_bases
GTGCCGGAAACGCCTCCTTTCAACATCGTACTGGTAGAACCGGAAATTCCGCCCAACACCGGCAACATCGCCCGGCTCTG
CGCCGCAACCGGAACCGTGCTGCACCTGGTGGAGCCGTTGGGTTTCTCCATCGACGACCGCCAGCTGAAGCGGGCAGGCC
TTGATTACTGGGGGAGTGTCACCCTCAAGGTCTGGCAGCGTCTGGAGCAGGTCTGGCAGGAATTTCCCCGGGGGCGTTTC
TTCTATCTCAGCACCAAGGCCGGACGGAGCTACCTGGCGGCGGATTTCAGGCCCGGCGATTTCATCGTCTTCGGCAAGGA
GACCAAAGGGTTGCCAGCCGAGATCCTGGAGTGGAACTCCGAGACAGCCATCACCATCCCCATGCCGGCCAACGCGGTGC
GCAGCCTGAATCTCTCCACCTCGGCCGGCATCGTGCTCTACGAGGCGCTGCGCCAAGCCGGGCAGATCGTCTGA

Upstream 100 bases:

>100_bases
TTGCTTTTGTTAGGGAAGTGGGCTAGGCTGTTCAGCTTGAAGCCGTACCAGGTCCGCAAGACGCCCTGGTGTCCAATAAC
CACTTTTTCAGAGGATAACC

Downstream 100 bases:

>100_bases
CGAAACCATCTCCCTGGGGGTAACCATGTCCGAGCAGCGCTATCTGACCGCCCATCTGCCGGGGTGCGGCGGGGTCATCA
AACAGTCTCCCGACGATTTC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MPETPPFNIVLVEPEIPPNTGNIARLCAATGTVLHLVEPLGFSIDDRQLKRAGLDYWGSVTLKVWQRLEQVWQEFPRGRF
FYLSTKAGRSYLAADFRPGDFIVFGKETKGLPAEILEWNSETAITIPMPANAVRSLNLSTSAGIVLYEALRQAGQIV

Sequences:

>Translated_157_residues
MPETPPFNIVLVEPEIPPNTGNIARLCAATGTVLHLVEPLGFSIDDRQLKRAGLDYWGSVTLKVWQRLEQVWQEFPRGRF
FYLSTKAGRSYLAADFRPGDFIVFGKETKGLPAEILEWNSETAITIPMPANAVRSLNLSTSAGIVLYEALRQAGQIV
>Mature_156_residues
PETPPFNIVLVEPEIPPNTGNIARLCAATGTVLHLVEPLGFSIDDRQLKRAGLDYWGSVTLKVWQRLEQVWQEFPRGRFF
YLSTKAGRSYLAADFRPGDFIVFGKETKGLPAEILEWNSETAITIPMPANAVRSLNLSTSAGIVLYEALRQAGQIV

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=149, Percent_Identity=47.6510067114094, Blast_Score=136, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17446; Mature: 17315

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETPPFNIVLVEPEIPPNTGNIARLCAATGTVLHLVEPLGFSIDDRQLKRAGLDYWGSV
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHH
TLKVWQRLEQVWQEFPRGRFFYLSTKAGRSYLAADFRPGDFIVFGKETKGLPAEILEWNS
HHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCCCHHHHCCCC
ETAITIPMPANAVRSLNLSTSAGIVLYEALRQAGQIV
CCEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PETPPFNIVLVEPEIPPNTGNIARLCAATGTVLHLVEPLGFSIDDRQLKRAGLDYWGSV
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHH
TLKVWQRLEQVWQEFPRGRFFYLSTKAGRSYLAADFRPGDFIVFGKETKGLPAEILEWNS
HHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCCCHHHHCCCC
ETAITIPMPANAVRSLNLSTSAGIVLYEALRQAGQIV
CCEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]