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The map label for this gene is tpiA

Identifier: 118580116

GI number: 118580116

Start: 1822115

End: 1822870

Strand: Direct

Name: tpiA

Synonym: Ppro_1695

Alternate gene names: 118580116

Gene position: 1822115-1822870 (Clockwise)

Preceding gene: 118580115

Following gene: 118580117

Centisome position: 45.46

GC content: 62.96

Gene sequence:

>756_bases
ATGCGCACGCCGCTGATCGCAGGAAACTGGAAACTGCACAAGACCATTGCCGAGTCGTTGGCCATGGTGGATGAACTGAA
ACCGCTGGTGGCGGGAAGCAGGGGGGTGGAGATCGTCGTTGCTCCGGTGTTCACCGCCCTGAAAAGCGTTTCGTTCGCTC
TGAACGGTAGTGACATCGGGCTTGCCGCCCAGGACTGCTTCTGGGAAGAACAGGGGGCCTTCACCGGGGAGGTGTCGCCG
GCGCAGCTCAGGGATGCGGGGTGCAGTCATGTGATCATCGGGCATTCCGAGCGCCGCCAGCTCTTCGGCGAGACCGATGA
GGGGGTGAATCGCAAGGCCAGGGCGGCCGTGGCCGTCGGCCTGACCGCCATCATCTGCGTAGGGGAAACCATGGAGGAGC
GGGAGAGTCGCGCCACCTTCACCGTGGTGGGCAGGCAGGTTACGGCCGCCCTGGCCGGCTTCTTGTCCCACGAATTTTCC
CGCATCGTCATCGCCTACGAACCGGTCTGGGCCATCGGAACCGGCAAGACCGCCACGGACCAGCAGGCCCAGGAGGTTCA
CTGCTATATCCGTAACCTGGTCACCATGTCCATCAGCCAGGCGATTGCCGATTCCCTGCGTATCCTCTACGGTGGCAGCG
TCAAACCGGACAACATCAGGGGACTCATGGCGCAACCGGACATAGATGGAGCCCTGATCGGCGGCGCCAGCCTGAAGGCC
GCCTCCTTTGCCGAGATGGTGAATTTCCGGGGATGA

Upstream 100 bases:

>100_bases
ATTTCCACCGGCGGTGGCGCGTTCCTGGAGCTGCTGGAGGGCAAGGTTCTGCCCGGCGTGAAGGCGCTGGACATCAAGGT
CAAGAAATAGGGGGGAGTCC

Downstream 100 bases:

>100_bases
ACCTCGTGGCGTACGGAGAAACCGAATAATTCCTTTACAATGGAATGGTGCGTATGGTAGAAGAGGTATTCTATTGCGCA
GGGGTTACTGAATGCTGATT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRTPLIAGNWKLHKTIAESLAMVDELKPLVAGSRGVEIVVAPVFTALKSVSFALNGSDIGLAAQDCFWEEQGAFTGEVSP
AQLRDAGCSHVIIGHSERRQLFGETDEGVNRKARAAVAVGLTAIICVGETMEERESRATFTVVGRQVTAALAGFLSHEFS
RIVIAYEPVWAIGTGKTATDQQAQEVHCYIRNLVTMSISQAIADSLRILYGGSVKPDNIRGLMAQPDIDGALIGGASLKA
ASFAEMVNFRG

Sequences:

>Translated_251_residues
MRTPLIAGNWKLHKTIAESLAMVDELKPLVAGSRGVEIVVAPVFTALKSVSFALNGSDIGLAAQDCFWEEQGAFTGEVSP
AQLRDAGCSHVIIGHSERRQLFGETDEGVNRKARAAVAVGLTAIICVGETMEERESRATFTVVGRQVTAALAGFLSHEFS
RIVIAYEPVWAIGTGKTATDQQAQEVHCYIRNLVTMSISQAIADSLRILYGGSVKPDNIRGLMAQPDIDGALIGGASLKA
ASFAEMVNFRG
>Mature_251_residues
MRTPLIAGNWKLHKTIAESLAMVDELKPLVAGSRGVEIVVAPVFTALKSVSFALNGSDIGLAAQDCFWEEQGAFTGEVSP
AQLRDAGCSHVIIGHSERRQLFGETDEGVNRKARAAVAVGLTAIICVGETMEERESRATFTVVGRQVTAALAGFLSHEFS
RIVIAYEPVWAIGTGKTATDQQAQEVHCYIRNLVTMSISQAIADSLRILYGGSVKPDNIRGLMAQPDIDGALIGGASLKA
ASFAEMVNFRG

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=254, Percent_Identity=43.3070866141732, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI226529917, Length=254, Percent_Identity=43.3070866141732, Blast_Score=195, Evalue=3e-50,
Organism=Escherichia coli, GI1790353, Length=248, Percent_Identity=45.5645161290323, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=45.7831325301205, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=38.5542168674699, Blast_Score=175, Evalue=8e-45,
Organism=Drosophila melanogaster, GI28572008, Length=257, Percent_Identity=44.7470817120623, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28572006, Length=257, Percent_Identity=44.7470817120623, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28572004, Length=257, Percent_Identity=44.7470817120623, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PELPD (A1APN8)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901366.1
- ProteinModelPortal:   A1APN8
- SMR:   A1APN8
- STRING:   A1APN8
- GeneID:   4572430
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1695
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   A1APN8
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26762; Mature: 26762

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPLIAGNWKLHKTIAESLAMVDELKPLVAGSRGVEIVVAPVFTALKSVSFALNGSDIG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHEEECCCCCC
LAAQDCFWEEQGAFTGEVSPAQLRDAGCSHVIIGHSERRQLFGETDEGVNRKARAAVAVG
EEHHHHCCCCCCCEECCCCHHHHHHCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHH
LTAIICVGETMEERESRATFTVVGRQVTAALAGFLSHEFSRIVIAYEPVWAIGTGKTATD
HHHEEECCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCEEEEEECCEEEEECCCCCCH
QQAQEVHCYIRNLVTMSISQAIADSLRILYGGSVKPDNIRGLMAQPDIDGALIGGASLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEECCCCCCEEECCCCCCH
ASFAEMVNFRG
HHHHHHHHCCC
>Mature Secondary Structure
MRTPLIAGNWKLHKTIAESLAMVDELKPLVAGSRGVEIVVAPVFTALKSVSFALNGSDIG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHEEECCCCCC
LAAQDCFWEEQGAFTGEVSPAQLRDAGCSHVIIGHSERRQLFGETDEGVNRKARAAVAVG
EEHHHHCCCCCCCEECCCCHHHHHHCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHH
LTAIICVGETMEERESRATFTVVGRQVTAALAGFLSHEFSRIVIAYEPVWAIGTGKTATD
HHHEEECCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCEEEEEECCEEEEECCCCCCH
QQAQEVHCYIRNLVTMSISQAIADSLRILYGGSVKPDNIRGLMAQPDIDGALIGGASLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEECCCCCCEEECCCCCCH
ASFAEMVNFRG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA