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The map label for this gene is eno

Identifier: 118580076

GI number: 118580076

Start: 1772459

End: 1773748

Strand: Reverse

Name: eno

Synonym: Ppro_1654

Alternate gene names: 118580076

Gene position: 1773748-1772459 (Counterclockwise)

Preceding gene: 118580084

Following gene: 118580074

Centisome position: 44.26

GC content: 61.09

Gene sequence:

>1290_bases
ATGAGTGAAATTGTTGATATCTATGCCAGAGAGATCCTGGATTCCCGCGGTAACCCCACCCTGGAGGTCGATGTATTCCT
GGAGTCGGGCGCCTTCGGGCGGGCCGCCGTGCCGTCCGGGGCATCCACCGGCGAGCGCGAGGCGCTGGAATTGCGCGACG
GCGACATGGCCCGCTATCTGGGCAAGGGTGTCCTCAAGGCAGTGGATAACGTCAACAACGAAATCGCCGACGAACTGATC
GGCATGGATGCGGACGACCAGGTCGCCATCGACAACAAGATGCTGGAGCTGGACGGAACCGAGTTCAAGAGCAAGCTGGG
CGCCAATGCCACCCTGGGAGTCTCCCTGGCCGTTGCCAAGGCCGCGGCCGACGAGGCCGGTCTGTCCCTGCACAAATACA
TCGGCGGAGCCGGAGCCCGCGAGCTGCCGCTGCCCATGATGAACATCTTGAACGGTGGCGCCCACGCCGACAACAACGTG
GATATCCAGGAATTCATGATCATGCCGGCCGGCGCCCGCTGCTTCGCCGAAGCGCTGCGCATGGGGGCGGAAATTTTCCA
CGCCCTGAAATCTGTGCTGAAGGGCAAGGGATACAACACCTCCGTGGGCGACGAGGGGGGCTTCGCGCCCAACCTGAAAT
CCAACGAAGAGGCCCTGCAGGTGATTATGGAGGCCATCGTCAAGGCCGGCTACAAGCCGGGTGAAGACGTGCTGCTGGCC
CTGGACGTGGCTTCGTCGGAGCTGTTCAAAGATGGCATTTATACCCTGGAAAACGAGCAGCAAAAGCAGAAAACCGCCGC
CCAGCTGATTGATTTCTACGAGGATCTGGTCAACCGCTATCCGATCATCTCCATCGAAGACGGCATGGCCGAGAACGACT
GGGATGGCTGGAAACTGCTCACCGACCGCCTGGGCAAGCGCATCCAGATCGTGGGGGACGACCTGTTCGTCACCAACCCC
AGGATCCTGAAAGAGGGCATCCAGAAAGGTATCGCCAACTCGATCCTGGTCAAGCTCAACCAGATCGGCACCCTCACCGA
GACGCTGGAGGCGATCGAAATGGCCAAGCGCGCCGGCTACACCACGGTCATCTCCCACCGTTCCGGGGAGACCGAGGACA
CCACCCTGGCCGACCTGGCGGTAGCGGTCAATGCCGGCCAGATCAAGACCGGTTCCCTCTGCCGCACCGACCGGGTGGCC
AAGTACAACCAGCTCTTGCGCATCGAAGAGGAACTGGACGACTGCGCCCTGTTCAGGGGGCATGACGTGTTTTACAACCT
GAAGAAGTAG

Upstream 100 bases:

>100_bases
AGCGTTTGTATTCTCTTGACGTTGTGTGCTATAGAGATACAGTTTTCCCACACGGAGATACTATCTCCCAAATCACCACT
ACACGCAAGGGGGCAGTATA

Downstream 100 bases:

>100_bases
GTTCCAATCAAAATACGGAAACACAAAAAGGCGGGATATCCCGCCTTTTTGTGTTTCCGCCGACAAAGCCGCATGAACGC
CCCCTACCCTGAAGCGCACA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSEIVDIYAREILDSRGNPTLEVDVFLESGAFGRAAVPSGASTGEREALELRDGDMARYLGKGVLKAVDNVNNEIADELI
GMDADDQVAIDNKMLELDGTEFKSKLGANATLGVSLAVAKAAADEAGLSLHKYIGGAGARELPLPMMNILNGGAHADNNV
DIQEFMIMPAGARCFAEALRMGAEIFHALKSVLKGKGYNTSVGDEGGFAPNLKSNEEALQVIMEAIVKAGYKPGEDVLLA
LDVASSELFKDGIYTLENEQQKQKTAAQLIDFYEDLVNRYPIISIEDGMAENDWDGWKLLTDRLGKRIQIVGDDLFVTNP
RILKEGIQKGIANSILVKLNQIGTLTETLEAIEMAKRAGYTTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVA
KYNQLLRIEEELDDCALFRGHDVFYNLKK

Sequences:

>Translated_429_residues
MSEIVDIYAREILDSRGNPTLEVDVFLESGAFGRAAVPSGASTGEREALELRDGDMARYLGKGVLKAVDNVNNEIADELI
GMDADDQVAIDNKMLELDGTEFKSKLGANATLGVSLAVAKAAADEAGLSLHKYIGGAGARELPLPMMNILNGGAHADNNV
DIQEFMIMPAGARCFAEALRMGAEIFHALKSVLKGKGYNTSVGDEGGFAPNLKSNEEALQVIMEAIVKAGYKPGEDVLLA
LDVASSELFKDGIYTLENEQQKQKTAAQLIDFYEDLVNRYPIISIEDGMAENDWDGWKLLTDRLGKRIQIVGDDLFVTNP
RILKEGIQKGIANSILVKLNQIGTLTETLEAIEMAKRAGYTTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVA
KYNQLLRIEEELDDCALFRGHDVFYNLKK
>Mature_428_residues
SEIVDIYAREILDSRGNPTLEVDVFLESGAFGRAAVPSGASTGEREALELRDGDMARYLGKGVLKAVDNVNNEIADELIG
MDADDQVAIDNKMLELDGTEFKSKLGANATLGVSLAVAKAAADEAGLSLHKYIGGAGARELPLPMMNILNGGAHADNNVD
IQEFMIMPAGARCFAEALRMGAEIFHALKSVLKGKGYNTSVGDEGGFAPNLKSNEEALQVIMEAIVKAGYKPGEDVLLAL
DVASSELFKDGIYTLENEQQKQKTAAQLIDFYEDLVNRYPIISIEDGMAENDWDGWKLLTDRLGKRIQIVGDDLFVTNPR
ILKEGIQKGIANSILVKLNQIGTLTETLEAIEMAKRAGYTTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVAK
YNQLLRIEEELDDCALFRGHDVFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=54.0603248259861, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=51.6129032258064, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=51.6129032258064, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=438, Percent_Identity=51.5981735159817, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=46.7592592592593, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=27.893175074184, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=27.893175074184, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=27.893175074184, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI310129182, Length=121, Percent_Identity=33.0578512396694, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI310110045, Length=121, Percent_Identity=33.0578512396694, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI310120572, Length=121, Percent_Identity=33.0578512396694, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=64.622641509434, Blast_Score=534, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.4722222222222, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.4722222222222, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=46.5968586387435, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=50.8009153318078, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.6535796766744, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.6535796766744, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.6535796766744, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=50.1144164759725, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=426, Percent_Identity=53.2863849765258, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580916, Length=426, Percent_Identity=53.2863849765258, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580920, Length=426, Percent_Identity=53.2863849765258, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580914, Length=426, Percent_Identity=53.2863849765258, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281360527, Length=426, Percent_Identity=53.2863849765258, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17137654, Length=426, Percent_Identity=53.2863849765258, Blast_Score=411, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PELPD (A1APJ8)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901326.1
- ProteinModelPortal:   A1APJ8
- SMR:   A1APJ8
- STRING:   A1APJ8
- GeneID:   4572294
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1654
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   A1APJ8
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46549; Mature: 46418

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 338-338 BINDING 155-155 BINDING 164-164 BINDING 286-286 BINDING 313-313 BINDING 338-338 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIVDIYAREILDSRGNPTLEVDVFLESGAFGRAAVPSGASTGEREALELRDGDMARYL
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHH
GKGVLKAVDNVNNEIADELIGMDADDQVAIDNKMLELDGTEFKSKLGANATLGVSLAVAK
HHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCEEEECCCHHHHHHHCCCCEEHHHHHHHH
AAADEAGLSLHKYIGGAGARELPLPMMNILNGGAHADNNVDIQEFMIMPAGARCFAEALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEECCCCHHHHHHHHH
MGAEIFHALKSVLKGKGYNTSVGDEGGFAPNLKSNEEALQVIMEAIVKAGYKPGEDVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSELFKDGIYTLENEQQKQKTAAQLIDFYEDLVNRYPIISIEDGMAENDWDGWKLL
EEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
TDRLGKRIQIVGDDLFVTNPRILKEGIQKGIANSILVKLNQIGTLTETLEAIEMAKRAGY
HHHCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVAKYNQLLRIEEELDDCALFRG
EEEEECCCCCCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
HDVFYNLKK
CCEEEECCC
>Mature Secondary Structure 
SEIVDIYAREILDSRGNPTLEVDVFLESGAFGRAAVPSGASTGEREALELRDGDMARYL
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHH
GKGVLKAVDNVNNEIADELIGMDADDQVAIDNKMLELDGTEFKSKLGANATLGVSLAVAK
HHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCEEEECCCHHHHHHHCCCCEEHHHHHHHH
AAADEAGLSLHKYIGGAGARELPLPMMNILNGGAHADNNVDIQEFMIMPAGARCFAEALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEECCCCHHHHHHHHH
MGAEIFHALKSVLKGKGYNTSVGDEGGFAPNLKSNEEALQVIMEAIVKAGYKPGEDVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSELFKDGIYTLENEQQKQKTAAQLIDFYEDLVNRYPIISIEDGMAENDWDGWKLL
EEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
TDRLGKRIQIVGDDLFVTNPRILKEGIQKGIANSILVKLNQIGTLTETLEAIEMAKRAGY
HHHCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVAKYNQLLRIEEELDDCALFRG
EEEEECCCCCCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
HDVFYNLKK
CCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA