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The map label for this gene is nuoN [H]

Identifier: 118580059

GI number: 118580059

Start: 1751994

End: 1753385

Strand: Direct

Name: nuoN [H]

Synonym: Ppro_1636

Alternate gene names: 118580059

Gene position: 1751994-1753385 (Clockwise)

Preceding gene: 118580058

Following gene: 118580060

Centisome position: 43.71

GC content: 62.28

Gene sequence:

>1392_bases
ATGGCCACTGATCTTCTGTACGGCCTTCTGCCGGAACACATCCTCTTGGGCCTGATCCTGGTCCTCATGCTGCTGGAGAT
ACTCAGCGTTGACAAGCGTGCCGGCAGCGCCCTGTTCATCGCCTCGCTCCTGGCCGGCGCCGGCGTCCTGGTCATGCAGC
TTCAAACCGGGTACACTGCCGACATCGTCATGAATGAGATCCGCATCGACCGCTTCTCCGAGATCGGCCGCCTGATCATC
GTAAGCTGCGGCGCCATCCTGGGGGTCTACTCCCTCTCCAGCGAAGCGGGGCACAAATACTGGATCCTCATCGCCTCATC
GCTTCTGGGCGCCATGATCATCCTGGACAGCGCCGGCTTCATCTCGCTCTTCATGGGCATCGAGATCCTCTCGCTCCCCG
GCTTCGCCCTGATGGTCCTGAATAACGGCAAATCAACCGCCTCCGAAGGCTCCATCAAGTATCTCCTGCTCTCCTCCGTG
GCCACGGCACTGGTCCTGTTCGGACTCTCCTTGGTGTACGGCTCTACCGGCAACCTGAACATCAGTTCCTTCACCGCAGC
CGTCGCCACCGGCGGCGTGCAGAACCTGGCCGCCAGCGTCATGATCCTCTCTGGCTTCTTCCTGAAAGCCTCCGTGTTCC
CCTTCCACGGCTGGGCACCCGACGCCTACTCCAGCGCCCGTCTGCCGGTAACGGCTTTCCTGGCCTCCATCGTCAAGGCC
GCCGTGGTGCTGGGCCTGGTGCGCATCCTGGGCAACGCCGTACTCAACCCGGAAGCGGTCACCGTGATTGCGCTCCTCTC
CATGCTCTCCATGTTCTACGGCAACATCACCGCCATCCACCAGACCGCCTTCAAGAAGATGCTGGCCTACTCCTCCATCT
CCCACGCCGGCTACATGATGTTCGCCCTGGTTGACAACACCGGAGCCAGAACCGAGGCGCTTCTGTACTACGTGGCCGTC
TACGCCGTCACCACCATCACCGCCTGCGCCTGCTTCAGCATCTTGAGCGGCGAGGACGACAACCTGGACAACCTGAACGG
CATCTTCAGGAAGAAGCCGGTGGCCGCCATCCTGCTTTCCCTGTGCGTACTCTCCCTGGCCGGCATCCCGCCGCTGCCAG
GCTTCCTGGCCAAGTTCTTCGTCTTCAAGACCGTCATCGCCTCCGGTCACCTGACCGTCGCCGTCCTGGCCTTCGTTGCC
AGCTACATCGGCACCTTCTTCTACCTGGGCGTGGTGCTGCGGATGTTCCGCTCCGACGCCGAAACCGTGGAGCAGCCAGC
CAACGCCACCTGCCTCTGCTGGACCTGGGGCGGAGCGCTCCTGGGTACCCTGGCCCTGGCACTGTTCATGCTGCTCCCCA
ATATCTTCCACTGGGTGATGACGGGATTGTAG

Upstream 100 bases:

>100_bases
GCGCTTGGCACCCAGCCGGCAAACGCCCAGATCCAACCGGCGGCGGCACAACCGGCGGCGTCACCCGCGGCGCCCACACC
ATCTGTCAAGGGAGGCGCCA

Downstream 100 bases:

>100_bases
GCACTGCAGCTATTCGTAGAGAGCCTGTGAAACACACAGCGCCCCTGTCCGAATCAACGTGCGGACAGGGGCGCTGTTTT
TGAACGTCAAGAGGGTGGGT

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MATDLLYGLLPEHILLGLILVLMLLEILSVDKRAGSALFIASLLAGAGVLVMQLQTGYTADIVMNEIRIDRFSEIGRLII
VSCGAILGVYSLSSEAGHKYWILIASSLLGAMIILDSAGFISLFMGIEILSLPGFALMVLNNGKSTASEGSIKYLLLSSV
ATALVLFGLSLVYGSTGNLNISSFTAAVATGGVQNLAASVMILSGFFLKASVFPFHGWAPDAYSSARLPVTAFLASIVKA
AVVLGLVRILGNAVLNPEAVTVIALLSMLSMFYGNITAIHQTAFKKMLAYSSISHAGYMMFALVDNTGARTEALLYYVAV
YAVTTITACACFSILSGEDDNLDNLNGIFRKKPVAAILLSLCVLSLAGIPPLPGFLAKFFVFKTVIASGHLTVAVLAFVA
SYIGTFFYLGVVLRMFRSDAETVEQPANATCLCWTWGGALLGTLALALFMLLPNIFHWVMTGL

Sequences:

>Translated_463_residues
MATDLLYGLLPEHILLGLILVLMLLEILSVDKRAGSALFIASLLAGAGVLVMQLQTGYTADIVMNEIRIDRFSEIGRLII
VSCGAILGVYSLSSEAGHKYWILIASSLLGAMIILDSAGFISLFMGIEILSLPGFALMVLNNGKSTASEGSIKYLLLSSV
ATALVLFGLSLVYGSTGNLNISSFTAAVATGGVQNLAASVMILSGFFLKASVFPFHGWAPDAYSSARLPVTAFLASIVKA
AVVLGLVRILGNAVLNPEAVTVIALLSMLSMFYGNITAIHQTAFKKMLAYSSISHAGYMMFALVDNTGARTEALLYYVAV
YAVTTITACACFSILSGEDDNLDNLNGIFRKKPVAAILLSLCVLSLAGIPPLPGFLAKFFVFKTVIASGHLTVAVLAFVA
SYIGTFFYLGVVLRMFRSDAETVEQPANATCLCWTWGGALLGTLALALFMLLPNIFHWVMTGL
>Mature_462_residues
ATDLLYGLLPEHILLGLILVLMLLEILSVDKRAGSALFIASLLAGAGVLVMQLQTGYTADIVMNEIRIDRFSEIGRLIIV
SCGAILGVYSLSSEAGHKYWILIASSLLGAMIILDSAGFISLFMGIEILSLPGFALMVLNNGKSTASEGSIKYLLLSSVA
TALVLFGLSLVYGSTGNLNISSFTAAVATGGVQNLAASVMILSGFFLKASVFPFHGWAPDAYSSARLPVTAFLASIVKAA
VVLGLVRILGNAVLNPEAVTVIALLSMLSMFYGNITAIHQTAFKKMLAYSSISHAGYMMFALVDNTGARTEALLYYVAVY
AVTTITACACFSILSGEDDNLDNLNGIFRKKPVAAILLSLCVLSLAGIPPLPGFLAKFFVFKTVIASGHLTVAVLAFVAS
YIGTFFYLGVVLRMFRSDAETVEQPANATCLCWTWGGALLGTLALALFMLLPNIFHWVMTGL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=356, Percent_Identity=33.9887640449438, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1788831, Length=294, Percent_Identity=27.5510204081633, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788614, Length=360, Percent_Identity=21.9444444444444, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788827, Length=309, Percent_Identity=22.9773462783171, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788829, Length=357, Percent_Identity=22.4089635854342, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788613, Length=308, Percent_Identity=27.5974025974026, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 49097; Mature: 48966

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDLLYGLLPEHILLGLILVLMLLEILSVDKRAGSALFIASLLAGAGVLVMQLQTGYTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCH
DIVMNEIRIDRFSEIGRLIIVSCGAILGVYSLSSEAGHKYWILIASSLLGAMIILDSAGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCHH
ISLFMGIEILSLPGFALMVLNNGKSTASEGSIKYLLLSSVATALVLFGLSLVYGSTGNLN
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ISSFTAAVATGGVQNLAASVMILSGFFLKASVFPFHGWAPDAYSSARLPVTAFLASIVKA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AVVLGLVRILGNAVLNPEAVTVIALLSMLSMFYGNITAIHQTAFKKMLAYSSISHAGYMM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEE
FALVDNTGARTEALLYYVAVYAVTTITACACFSILSGEDDNLDNLNGIFRKKPVAAILLS
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
LCVLSLAGIPPLPGFLAKFFVFKTVIASGHLTVAVLAFVASYIGTFFYLGVVLRMFRSDA
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ETVEQPANATCLCWTWGGALLGTLALALFMLLPNIFHWVMTGL
HHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATDLLYGLLPEHILLGLILVLMLLEILSVDKRAGSALFIASLLAGAGVLVMQLQTGYTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCH
DIVMNEIRIDRFSEIGRLIIVSCGAILGVYSLSSEAGHKYWILIASSLLGAMIILDSAGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCHH
ISLFMGIEILSLPGFALMVLNNGKSTASEGSIKYLLLSSVATALVLFGLSLVYGSTGNLN
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ISSFTAAVATGGVQNLAASVMILSGFFLKASVFPFHGWAPDAYSSARLPVTAFLASIVKA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AVVLGLVRILGNAVLNPEAVTVIALLSMLSMFYGNITAIHQTAFKKMLAYSSISHAGYMM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEE
FALVDNTGARTEALLYYVAVYAVTTITACACFSILSGEDDNLDNLNGIFRKKPVAAILLS
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
LCVLSLAGIPPLPGFLAKFFVFKTVIASGHLTVAVLAFVASYIGTFFYLGVVLRMFRSDA
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ETVEQPANATCLCWTWGGALLGTLALALFMLLPNIFHWVMTGL
HHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA