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The map label for this gene is thiH [H]

Identifier: 118579916

GI number: 118579916

Start: 1600358

End: 1601779

Strand: Direct

Name: thiH [H]

Synonym: Ppro_1492

Alternate gene names: 118579916

Gene position: 1600358-1601779 (Clockwise)

Preceding gene: 118579915

Following gene: 118579917

Centisome position: 39.93

GC content: 60.34

Gene sequence:

>1422_bases
ATGAGTTCCCTGCCATCCATGAAACTGAGCGCAGGCGCCGCCGACTTCATCGACGAGGCGTATCTGTTCGGCCTTCTGGG
AGGCAAGCGTCCCGAAGCGGCACGGGTTCGCGACATAATCGCCAAGAGCCTTGAAAAGCAGGCACTGGGTGTGGAGGAAA
CCGCGCAGCTTCTGCTGGCGGATACGCCTGAGCTGGTCGAGGAGATCTTTGCCGCGGCCCGCGAACTGAAAGAAAAGGTG
TACGGAAACAGGATCGTCCTGTTCGCCCCGCTCTACATCGGGAATAAATGTGTGAACGACTGCGCCTACTGCGCCTTCAA
GCGGAGCAATGCCAGCGCAATCCGGCGCACGCTGACCGAAGGCGAGATCCGCGGGCAGGTCGAAGCCCTGGAAAGCAAGG
GGCACAAACGGCTGATCCTGGTGTTCGGCGAACACCAGGCCTATGATGCCGAGTTCATCGCCCAGAGCGTCAGGACCGTC
TACGACACCAGGGGAAAGGGTGAGATCAGGCGGGTGAACATCAATGCCGCCCCCCTTGACCGGGAGGGGTATGCCATTGT
CAAGGCCGCCGGGATCGGCACCTACCAGATCTTTCACGAAACATACCATCACGAAACCTATGCCCGCCTGCATCCGCGGG
GCACCCGCAAGGCAAACTACCTCTACCGACTGGACGGACTCAACCGGGCCTTTGAGGCCGGCTGCGACGATGTGGGGCTG
GGGGTGCTCTTTGGTCTTGTGGATTGGAGATTCGAGGTGCTGGGCCTGGTCAGCCACGCCCTTCACCTGCAGCGGCGCTA
CAATGTGGGTCCCCACACCATCAGTTTTCCGCGCCTGCGGCCTGCCGCGGGTGTTGCGCTGGACAGGGAGCTCTTGGTTG
GCGACCAGGAATTCAAGCGGATCATCGCCATACTCCGTCTGGCTGTGCCCTATACCGGGCTCATCCTGACCGCCCGGGAA
AATGCCCAGGTACGCCGGGAATGCATCTCCTTAGGCGTTTCCCAGATCGACGCCGGCAGCCGCATCGAGCTGGGCGGCTA
TACCGAGGCCGGGGATGCTCAGGTGATGGAGCGGGAACAGTTCTCACTTGGCGACGTGCGTTCCCTGGACGAGGTCATGG
CCGAACTGATGGGAGCTGGCTATATTCCCAGTTTCTGCACCTCCTGCTACCGGGTCGGCAGAACCGGTGAACATTTCATG
GAATTCAGCATCCCCGGCTTCATAAAGGAATTCTGCACGCCCAATGCCCTGCTGACGCTGCAGGAATACTTGCTGGACTA
TGCGTCACCGTCCACCAGGGAAATCGGAGACAGGCTGATAGCGGAAGAACTGGCCAAGCTGGTCGATGGCCATGGTAAAC
AGGGCGTGGTGCAGAGGCTCAAAGAAATTAGGGAGGGCGCACAACGCGACCACTGTTTCTGA

Upstream 100 bases:

>100_bases
TCGTGCGCAGGATTCGCTCCATGGGCAGAACCATCGGCAGGGGGCGCGGCGATTCACCCAACCTGTCCGGGCGAAAGAGC
CGCACGAGCGAGGTGCTGCC

Downstream 100 bases:

>100_bases
GCGGTACAAAAAGACGTGGCTGCGGACAAACCATGAAACCCTTTCGTAGCGAAAAAGACCTCCTGGGAGAACGGGAAATT
CCCGTTGATGCGCTGTACGG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSSLPSMKLSAGAADFIDEAYLFGLLGGKRPEAARVRDIIAKSLEKQALGVEETAQLLLADTPELVEEIFAAARELKEKV
YGNRIVLFAPLYIGNKCVNDCAYCAFKRSNASAIRRTLTEGEIRGQVEALESKGHKRLILVFGEHQAYDAEFIAQSVRTV
YDTRGKGEIRRVNINAAPLDREGYAIVKAAGIGTYQIFHETYHHETYARLHPRGTRKANYLYRLDGLNRAFEAGCDDVGL
GVLFGLVDWRFEVLGLVSHALHLQRRYNVGPHTISFPRLRPAAGVALDRELLVGDQEFKRIIAILRLAVPYTGLILTARE
NAQVRRECISLGVSQIDAGSRIELGGYTEAGDAQVMEREQFSLGDVRSLDEVMAELMGAGYIPSFCTSCYRVGRTGEHFM
EFSIPGFIKEFCTPNALLTLQEYLLDYASPSTREIGDRLIAEELAKLVDGHGKQGVVQRLKEIREGAQRDHCF

Sequences:

>Translated_473_residues
MSSLPSMKLSAGAADFIDEAYLFGLLGGKRPEAARVRDIIAKSLEKQALGVEETAQLLLADTPELVEEIFAAARELKEKV
YGNRIVLFAPLYIGNKCVNDCAYCAFKRSNASAIRRTLTEGEIRGQVEALESKGHKRLILVFGEHQAYDAEFIAQSVRTV
YDTRGKGEIRRVNINAAPLDREGYAIVKAAGIGTYQIFHETYHHETYARLHPRGTRKANYLYRLDGLNRAFEAGCDDVGL
GVLFGLVDWRFEVLGLVSHALHLQRRYNVGPHTISFPRLRPAAGVALDRELLVGDQEFKRIIAILRLAVPYTGLILTARE
NAQVRRECISLGVSQIDAGSRIELGGYTEAGDAQVMEREQFSLGDVRSLDEVMAELMGAGYIPSFCTSCYRVGRTGEHFM
EFSIPGFIKEFCTPNALLTLQEYLLDYASPSTREIGDRLIAEELAKLVDGHGKQGVVQRLKEIREGAQRDHCF
>Mature_472_residues
SSLPSMKLSAGAADFIDEAYLFGLLGGKRPEAARVRDIIAKSLEKQALGVEETAQLLLADTPELVEEIFAAARELKEKVY
GNRIVLFAPLYIGNKCVNDCAYCAFKRSNASAIRRTLTEGEIRGQVEALESKGHKRLILVFGEHQAYDAEFIAQSVRTVY
DTRGKGEIRRVNINAAPLDREGYAIVKAAGIGTYQIFHETYHHETYARLHPRGTRKANYLYRLDGLNRAFEAGCDDVGLG
VLFGLVDWRFEVLGLVSHALHLQRRYNVGPHTISFPRLRPAAGVALDRELLVGDQEFKRIIAILRLAVPYTGLILTAREN
AQVRRECISLGVSQIDAGSRIELGGYTEAGDAQVMEREQFSLGDVRSLDEVMAELMGAGYIPSFCTSCYRVGRTGEHFME
FSIPGFIKEFCTPNALLTLQEYLLDYASPSTREIGDRLIAEELAKLVDGHGKQGVVQRLKEIREGAQRDHCF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=360, Percent_Identity=31.1111111111111, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 52637; Mature: 52506

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLPSMKLSAGAADFIDEAYLFGLLGGKRPEAARVRDIIAKSLEKQALGVEETAQLLLA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
DTPELVEEIFAAARELKEKVYGNRIVLFAPLYIGNKCVNDCAYCAFKRSNASAIRRTLTE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHCHHHHHHHHHHHHCCCCHHHHHHHHHH
GEIRGQVEALESKGHKRLILVFGEHQAYDAEFIAQSVRTVYDTRGKGEIRRVNINAAPLD
HHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
REGYAIVKAAGIGTYQIFHETYHHETYARLHPRGTRKANYLYRLDGLNRAFEAGCDDVGL
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHCCCCCCHH
GVLFGLVDWRFEVLGLVSHALHLQRRYNVGPHTISFPRLRPAAGVALDRELLVGDQEFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHH
IIAILRLAVPYTGLILTARENAQVRRECISLGVSQIDAGSRIELGGYTEAGDAQVMEREQ
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCHHHCCCCCEEEECCCCCCCCHHHHHHHH
FSLGDVRSLDEVMAELMGAGYIPSFCTSCYRVGRTGEHFMEFSIPGFIKEFCTPNALLTL
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCCCHHHHH
QEYLLDYASPSTREIGDRLIAEELAKLVDGHGKQGVVQRLKEIREGAQRDHCF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSLPSMKLSAGAADFIDEAYLFGLLGGKRPEAARVRDIIAKSLEKQALGVEETAQLLLA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
DTPELVEEIFAAARELKEKVYGNRIVLFAPLYIGNKCVNDCAYCAFKRSNASAIRRTLTE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHCHHHHHHHHHHHHCCCCHHHHHHHHHH
GEIRGQVEALESKGHKRLILVFGEHQAYDAEFIAQSVRTVYDTRGKGEIRRVNINAAPLD
HHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
REGYAIVKAAGIGTYQIFHETYHHETYARLHPRGTRKANYLYRLDGLNRAFEAGCDDVGL
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHCCCCCCHH
GVLFGLVDWRFEVLGLVSHALHLQRRYNVGPHTISFPRLRPAAGVALDRELLVGDQEFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHH
IIAILRLAVPYTGLILTARENAQVRRECISLGVSQIDAGSRIELGGYTEAGDAQVMEREQ
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCHHHCCCCCEEEECCCCCCCCHHHHHHHH
FSLGDVRSLDEVMAELMGAGYIPSFCTSCYRVGRTGEHFMEFSIPGFIKEFCTPNALLTL
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCCCHHHHH
QEYLLDYASPSTREIGDRLIAEELAKLVDGHGKQGVVQRLKEIREGAQRDHCF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]