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The map label for this gene is aspA [H]

Identifier: 118579917

GI number: 118579917

Start: 1601812

End: 1603227

Strand: Direct

Name: aspA [H]

Synonym: Ppro_1493

Alternate gene names: 118579917

Gene position: 1601812-1603227 (Clockwise)

Preceding gene: 118579916

Following gene: 118579918

Centisome position: 39.97

GC content: 62.85

Gene sequence:

>1416_bases
ATGAAACCCTTTCGTAGCGAAAAAGACCTCCTGGGAGAACGGGAAATTCCCGTTGATGCGCTGTACGGCATTCACACCCT
GCGCGGGATGGAAAACTTTCCGCTTGCCCGTCGAACGGTGCATGGAGAGCTGATCCATGCCTATGGCGCCGTCAAGCTGG
CCTGCGCACGAACCAATCATGCTCTCGGCTGGTGGGATCAACCCAAGGCCGATGCCATACAACAGGCCTGCCGGGAAATG
ATGGCGGGACAACTGGATCGCCATATCATGGTGGATGCCCTGCAGGGCGGGGCGGGGACCTCCACCAATATGAATGTCAA
CGAGGTGCTGGCCAACCGTGCCCTGCAGCTGTTAGGCAGGTCACCTGGTGACTACGCAATTATCAGCCCGCTGGAAGACC
TGAATCTGCACCAGTCTACCAACGACACCTATCCCACGGCACTCAAGGTGGCTGCAATCACGCGGCTCAGGGCGCTGGAG
CAGGAGCTTATCGGGTTGCTGGAAGCGTTTCAGGAACGGGAGCGGGCCTTCAGCCACATCGTCAAGGTGGGACGAACCCA
GTATCAGGATGCCGTCCTGACCACATTGGGACGGTCCATGTCGGCCTATGCCGAAGCGTTCGGTCGCGACCGCTGGCGGG
TCTACAAGTGCGAGGAACGCCTGCGGGTCGTGAATCTGGGGGGAACCGCCATCGGTACCGGCCTGGCCGCACCGCGCCAA
TATATTTTCCAGGTGGTCGAGCAGTTGCGTGCCATCACCGGGCTGGGGCTGGCACGGGCCGAGAACCTGGTGGATGCGAC
CCAGAACACGGACGTCTTTGTGGAAGCCAGCGGCATGCTGAAGGCATGCGCCAGCAACCTGCTCAAGGTGGCGACTGACC
TGCGGCTCCTGTCGTCCGGTCCGGATGCGGGCCTGGGGGAGCTGCGTCTTCCTGCTCGCCAGGCCGGTTCCTCGATCATG
CCGGGCAAGGTCAACCCGGTCATACCCGAGGCCGTGTCCCAGGCGGCCATGAGTGTCATCGCCAATGATCAGGCAATCAC
CCTGGCCTGCTCCCTGGGCAGTCTGGAGCTGAACGCCTTCCTGCCGCTGATCGCCGATGCCCTGCTGGGGAGCCTGGACC
TGCTGGTTAATGCCTGCTCCCTGTTCAGCCGTCTGTGCGTGGCAGGGCTGGAGGCAGGGGAGGCCCGCTGTCTCCGGCAT
GTGGAAAATGCCACCGCCAGTATCACGGCGCTGGTGGATGCCGTCGGGTATCAGGCTGCCCAGGAGATCGCGGGGGACAT
GGCGGATGGGGGAAGTGTCCGTGAGGCCGTGGTTCGGCGCGGCCTGATGACGGCTGAAACCTTTGACGAGCTTGTCTCGG
CTGAATCCGTCACGCGGCTTGGTTCACCGCTGCCGGGGGAGAGGGGACGACAGTGA

Upstream 100 bases:

>100_bases
GTAAACAGGGCGTGGTGCAGAGGCTCAAAGAAATTAGGGAGGGCGCACAACGCGACCACTGTTTCTGAGCGGTACAAAAA
GACGTGGCTGCGGACAAACC

Downstream 100 bases:

>100_bases
AAGCCACACCAAAGGGGAACCGTCTCCACATCGGCCTGTTCGGACGCCGCAATGTGGGCAAATCCTCTCTTCTGAACTCC
CTGACCCGCCAGGATGCAGC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKPFRSEKDLLGEREIPVDALYGIHTLRGMENFPLARRTVHGELIHAYGAVKLACARTNHALGWWDQPKADAIQQACREM
MAGQLDRHIMVDALQGGAGTSTNMNVNEVLANRALQLLGRSPGDYAIISPLEDLNLHQSTNDTYPTALKVAAITRLRALE
QELIGLLEAFQERERAFSHIVKVGRTQYQDAVLTTLGRSMSAYAEAFGRDRWRVYKCEERLRVVNLGGTAIGTGLAAPRQ
YIFQVVEQLRAITGLGLARAENLVDATQNTDVFVEASGMLKACASNLLKVATDLRLLSSGPDAGLGELRLPARQAGSSIM
PGKVNPVIPEAVSQAAMSVIANDQAITLACSLGSLELNAFLPLIADALLGSLDLLVNACSLFSRLCVAGLEAGEARCLRH
VENATASITALVDAVGYQAAQEIAGDMADGGSVREAVVRRGLMTAETFDELVSAESVTRLGSPLPGERGRQ

Sequences:

>Translated_471_residues
MKPFRSEKDLLGEREIPVDALYGIHTLRGMENFPLARRTVHGELIHAYGAVKLACARTNHALGWWDQPKADAIQQACREM
MAGQLDRHIMVDALQGGAGTSTNMNVNEVLANRALQLLGRSPGDYAIISPLEDLNLHQSTNDTYPTALKVAAITRLRALE
QELIGLLEAFQERERAFSHIVKVGRTQYQDAVLTTLGRSMSAYAEAFGRDRWRVYKCEERLRVVNLGGTAIGTGLAAPRQ
YIFQVVEQLRAITGLGLARAENLVDATQNTDVFVEASGMLKACASNLLKVATDLRLLSSGPDAGLGELRLPARQAGSSIM
PGKVNPVIPEAVSQAAMSVIANDQAITLACSLGSLELNAFLPLIADALLGSLDLLVNACSLFSRLCVAGLEAGEARCLRH
VENATASITALVDAVGYQAAQEIAGDMADGGSVREAVVRRGLMTAETFDELVSAESVTRLGSPLPGERGRQ
>Mature_471_residues
MKPFRSEKDLLGEREIPVDALYGIHTLRGMENFPLARRTVHGELIHAYGAVKLACARTNHALGWWDQPKADAIQQACREM
MAGQLDRHIMVDALQGGAGTSTNMNVNEVLANRALQLLGRSPGDYAIISPLEDLNLHQSTNDTYPTALKVAAITRLRALE
QELIGLLEAFQERERAFSHIVKVGRTQYQDAVLTTLGRSMSAYAEAFGRDRWRVYKCEERLRVVNLGGTAIGTGLAAPRQ
YIFQVVEQLRAITGLGLARAENLVDATQNTDVFVEASGMLKACASNLLKVATDLRLLSSGPDAGLGELRLPARQAGSSIM
PGKVNPVIPEAVSQAAMSVIANDQAITLACSLGSLELNAFLPLIADALLGSLDLLVNACSLFSRLCVAGLEAGEARCLRH
VENATASITALVDAVGYQAAQEIAGDMADGGSVREAVVRRGLMTAETFDELVSAESVTRLGSPLPGERGRQ

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=458, Percent_Identity=38.2096069868996, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI87082375, Length=459, Percent_Identity=41.6122004357298, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=38.3442265795207, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17553882, Length=464, Percent_Identity=38.5775862068966, Blast_Score=297, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI32565146, Length=325, Percent_Identity=37.5384615384615, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324993, Length=458, Percent_Identity=39.0829694323144, Blast_Score=318, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24640177, Length=459, Percent_Identity=36.1655773420479, Blast_Score=291, Evalue=5e-79,
Organism=Drosophila melanogaster, GI24640179, Length=459, Percent_Identity=36.1655773420479, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI78710009, Length=460, Percent_Identity=36.7391304347826, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI24662684, Length=458, Percent_Identity=35.8078602620087, Blast_Score=278, Evalue=7e-75,
Organism=Drosophila melanogaster, GI24583245, Length=457, Percent_Identity=33.9168490153173, Blast_Score=258, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50597; Mature: 50597

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFRSEKDLLGEREIPVDALYGIHTLRGMENFPLARRTVHGELIHAYGAVKLACARTNH
CCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCC
ALGWWDQPKADAIQQACREMMAGQLDRHIMVDALQGGAGTSTNMNVNEVLANRALQLLGR
CCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
SPGDYAIISPLEDLNLHQSTNDTYPTALKVAAITRLRALEQELIGLLEAFQERERAFSHI
CCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKVGRTQYQDAVLTTLGRSMSAYAEAFGRDRWRVYKCEERLRVVNLGGTAIGTGLAAPRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCEEEECCCHHHHCCCCHHHH
YIFQVVEQLRAITGLGLARAENLVDATQNTDVFVEASGMLKACASNLLKVATDLRLLSSG
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCC
PDAGLGELRLPARQAGSSIMPGKVNPVIPEAVSQAAMSVIANDQAITLACSLGSLELNAF
CCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHH
LPLIADALLGSLDLLVNACSLFSRLCVAGLEAGEARCLRHVENATASITALVDAVGYQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEIAGDMADGGSVREAVVRRGLMTAETFDELVSAESVTRLGSPLPGERGRQ
HHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKPFRSEKDLLGEREIPVDALYGIHTLRGMENFPLARRTVHGELIHAYGAVKLACARTNH
CCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCC
ALGWWDQPKADAIQQACREMMAGQLDRHIMVDALQGGAGTSTNMNVNEVLANRALQLLGR
CCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
SPGDYAIISPLEDLNLHQSTNDTYPTALKVAAITRLRALEQELIGLLEAFQERERAFSHI
CCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKVGRTQYQDAVLTTLGRSMSAYAEAFGRDRWRVYKCEERLRVVNLGGTAIGTGLAAPRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCEEEECCCHHHHCCCCHHHH
YIFQVVEQLRAITGLGLARAENLVDATQNTDVFVEASGMLKACASNLLKVATDLRLLSSG
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCC
PDAGLGELRLPARQAGSSIMPGKVNPVIPEAVSQAAMSVIANDQAITLACSLGSLELNAF
CCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHH
LPLIADALLGSLDLLVNACSLFSRLCVAGLEAGEARCLRHVENATASITALVDAVGYQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEIAGDMADGGSVREAVVRRGLMTAETFDELVSAESVTRLGSPLPGERGRQ
HHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]