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The map label for this gene is btuB [H]

Identifier: 118579896

GI number: 118579896

Start: 1573382

End: 1575433

Strand: Direct

Name: btuB [H]

Synonym: Ppro_1472

Alternate gene names: 118579896

Gene position: 1573382-1575433 (Clockwise)

Preceding gene: 118579895

Following gene: 118579897

Centisome position: 39.26

GC content: 56.14

Gene sequence:

>2052_bases
ATGAAGAAACAGAAGAGACGTTTTTCCGTCAGAATCAAGAAACGGGGTCTGCTGGCACCGGTGCTGGCGGTTTCCCTGCT
GTCGACACCAGCCATGGCCGATGACCAGGCGCCGAAGAGTGACGAAAGCGGCGATGTCTTCACCCTGGGCAACGTGGAGG
TTACCGGCAAGGGCGAGAGCATCAAGAACCCGGCCGTGGAGGTGGTCACCAGCGAGGATATGCTCCAGTTCGACACCAAG
ACCGTGTCCGAAGCAGCCAACCTGGTTCCCGGTGTCACCCTTGCCAAGATGGGGGCGCGCAACGAGTCCACGGTCTACGT
GCGCGGTTTCGACCTCAAACACGCGCCGCTGTTCATGGACGGCATCCCGATCTACGTCCCCTATGACGGCAATGTGGACC
TGGGGCGCTTCTTCACCTACGACCTGTCGGAGCTGGTCATCTCCAAGGGGTTTGCGTCGGTGCTCTACGGTCCCAACACC
ATGGGGGGCGCCATCAACATGGTCTCCAAGCGCCCCACCAAGGAGTTCGAGGGCAGTGCCGGCACCGGTTACAGCTCCGT
GGACCAGTACCACGCCTTCGCCAACTTCGGAACCAACCAGGGAAAATGGTACTTCCAGGGAGGCGGCTCCTGGTTCGACA
CCAACGGCTTCGAGATGTCGGAGCACTACCACAACACAACCTACGAGAACGGCGGCAAGCGCAACAACGCCTATGCCCAG
GACTGGAAGGTCAGCGGTAAGCTGGGGTTGACCCCCAACAGCACCGACGAGTACGCCCTGAGCTATATCTACCAGAACGG
CGAAAAGGGGGTGCCTCCCTACGCCGGCGACAATCTCACAAACCAGAAAAAGAAGTTCTGGCAGTGGCCCTACTGGAGAA
AGCAGAGCCTGTACTTCACCTCCAATACTGCCATCGGCGAGAAGAGTTATGTGAAGACCCGCGCCTATTATGATCGCTTC
AAAAACTCGCTTTTGATCTTCACCAATGACAGCTACAGCACCGTCACGCCCAACAGCGGCAACAGGAGCTGGTACGACGA
CTACACCTATGGCGCGTCCATCGAGGCTGGCACCAAACTGATCTCCAACAACGAAATCAAGCTGGCCTTTCTTTTCAAGG
ACGATGTCCATCGTGAGGACGATGATGCCACCGGTTCCCCACAGAAACGTTTTGAGGAGCGCATCTTCACCATCGCCCTG
GAGGACACGATCAAGCTGACCGACAAGCTCTCCGCCATACTGGGGGTCAGCTACGACTTCCAGAATACGGTCCAGGCCCA
GGACTACAATCCGATCGTCTCCGGCGGCCCCAATGTGCTGCACGATTTCGACCGTAAAAACAATAGCGACGTCAATGCCC
AGGCAGCGCTGTTCTATGCCTACTCCGACACCGGCAAGGTGCATGCCGCCGTGGCGCGCAAGAATCGTTACCCTTCCATC
AAGGATAAATACTCCTACCGGTTCAAGACCTATGTGCCAAACGAAAATCTGGATCCTGAAGTGTCCAATAACTATGAAAT
CGGTGTCGAGGATACATTTGCCAAAAATATTAGAGTCAAAAGCACCCTGTTTTTTAACGACATATCGAAATATATCCTGG
AAGTTAAAAACGTGGAGGGAACCAAGTCGCAGCTTCAGAACGTTGGCCACGTCCAGCGCTACGGCTACGAACTCGAGGCG
AACGCCGCTATTACCGAGAGCCTGGAAGCCGGTATGAACTATACCTTCATGTACAACAAGAACAGGACCAGCGATCTGGT
AATAACCGATCTTCCCAAGCACAAGTTCTTCGCCTACGGCACGTACTCCCCCATCAAGCAACTCAAGCTGTTCGCCTCAC
TGGAGTACGACACCAGGCGCTACAGTTCGTCCGACGGGGTGGATGAGGCGGGTGAGTTTGTGGTCATCAACACCAAGGCG
AGTTACGAGGCGATCAAGGACCTCGTGCTGGAGGCGGGGATCAACAACCTCCTGGACCGGGATTATGAGTTGACCGAGGG
CTATCCCGAGGCGGGCCGCACCTATTTCGTCCAGGCGCGCTACAAGTGGTGA

Upstream 100 bases:

>100_bases
AGGACAAGGAACAAGGCGTGGTTGACCACTGCCCCGGGTACTTCGCGTAACGCGACAATGCACTGCAATGATGCTGTGAA
CACACCATGAGGAGGAATCA

Downstream 100 bases:

>100_bases
GACGGTACTAGCTGAAAGACAAGGGACGTGACTCGTGACTGGTGACTGGGCCAGCCACGAGTCACCAGCCACGAGTTACG
AGTTACGAGTTACGGGGAGA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MKKQKRRFSVRIKKRGLLAPVLAVSLLSTPAMADDQAPKSDESGDVFTLGNVEVTGKGESIKNPAVEVVTSEDMLQFDTK
TVSEAANLVPGVTLAKMGARNESTVYVRGFDLKHAPLFMDGIPIYVPYDGNVDLGRFFTYDLSELVISKGFASVLYGPNT
MGGAINMVSKRPTKEFEGSAGTGYSSVDQYHAFANFGTNQGKWYFQGGGSWFDTNGFEMSEHYHNTTYENGGKRNNAYAQ
DWKVSGKLGLTPNSTDEYALSYIYQNGEKGVPPYAGDNLTNQKKKFWQWPYWRKQSLYFTSNTAIGEKSYVKTRAYYDRF
KNSLLIFTNDSYSTVTPNSGNRSWYDDYTYGASIEAGTKLISNNEIKLAFLFKDDVHREDDDATGSPQKRFEERIFTIAL
EDTIKLTDKLSAILGVSYDFQNTVQAQDYNPIVSGGPNVLHDFDRKNNSDVNAQAALFYAYSDTGKVHAAVARKNRYPSI
KDKYSYRFKTYVPNENLDPEVSNNYEIGVEDTFAKNIRVKSTLFFNDISKYILEVKNVEGTKSQLQNVGHVQRYGYELEA
NAAITESLEAGMNYTFMYNKNRTSDLVITDLPKHKFFAYGTYSPIKQLKLFASLEYDTRRYSSSDGVDEAGEFVVINTKA
SYEAIKDLVLEAGINNLLDRDYELTEGYPEAGRTYFVQARYKW

Sequences:

>Translated_683_residues
MKKQKRRFSVRIKKRGLLAPVLAVSLLSTPAMADDQAPKSDESGDVFTLGNVEVTGKGESIKNPAVEVVTSEDMLQFDTK
TVSEAANLVPGVTLAKMGARNESTVYVRGFDLKHAPLFMDGIPIYVPYDGNVDLGRFFTYDLSELVISKGFASVLYGPNT
MGGAINMVSKRPTKEFEGSAGTGYSSVDQYHAFANFGTNQGKWYFQGGGSWFDTNGFEMSEHYHNTTYENGGKRNNAYAQ
DWKVSGKLGLTPNSTDEYALSYIYQNGEKGVPPYAGDNLTNQKKKFWQWPYWRKQSLYFTSNTAIGEKSYVKTRAYYDRF
KNSLLIFTNDSYSTVTPNSGNRSWYDDYTYGASIEAGTKLISNNEIKLAFLFKDDVHREDDDATGSPQKRFEERIFTIAL
EDTIKLTDKLSAILGVSYDFQNTVQAQDYNPIVSGGPNVLHDFDRKNNSDVNAQAALFYAYSDTGKVHAAVARKNRYPSI
KDKYSYRFKTYVPNENLDPEVSNNYEIGVEDTFAKNIRVKSTLFFNDISKYILEVKNVEGTKSQLQNVGHVQRYGYELEA
NAAITESLEAGMNYTFMYNKNRTSDLVITDLPKHKFFAYGTYSPIKQLKLFASLEYDTRRYSSSDGVDEAGEFVVINTKA
SYEAIKDLVLEAGINNLLDRDYELTEGYPEAGRTYFVQARYKW
>Mature_683_residues
MKKQKRRFSVRIKKRGLLAPVLAVSLLSTPAMADDQAPKSDESGDVFTLGNVEVTGKGESIKNPAVEVVTSEDMLQFDTK
TVSEAANLVPGVTLAKMGARNESTVYVRGFDLKHAPLFMDGIPIYVPYDGNVDLGRFFTYDLSELVISKGFASVLYGPNT
MGGAINMVSKRPTKEFEGSAGTGYSSVDQYHAFANFGTNQGKWYFQGGGSWFDTNGFEMSEHYHNTTYENGGKRNNAYAQ
DWKVSGKLGLTPNSTDEYALSYIYQNGEKGVPPYAGDNLTNQKKKFWQWPYWRKQSLYFTSNTAIGEKSYVKTRAYYDRF
KNSLLIFTNDSYSTVTPNSGNRSWYDDYTYGASIEAGTKLISNNEIKLAFLFKDDVHREDDDATGSPQKRFEERIFTIAL
EDTIKLTDKLSAILGVSYDFQNTVQAQDYNPIVSGGPNVLHDFDRKNNSDVNAQAALFYAYSDTGKVHAAVARKNRYPSI
KDKYSYRFKTYVPNENLDPEVSNNYEIGVEDTFAKNIRVKSTLFFNDISKYILEVKNVEGTKSQLQNVGHVQRYGYELEA
NAAITESLEAGMNYTFMYNKNRTSDLVITDLPKHKFFAYGTYSPIKQLKLFASLEYDTRRYSSSDGVDEAGEFVVINTKA
SYEAIKDLVLEAGINNLLDRDYELTEGYPEAGRTYFVQARYKW

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=181, Percent_Identity=27.0718232044199, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 77044; Mature: 77044

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQKRRFSVRIKKRGLLAPVLAVSLLSTPAMADDQAPKSDESGDVFTLGNVEVTGKGES
CCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCC
IKNPAVEVVTSEDMLQFDTKTVSEAANLVPGVTLAKMGARNESTVYVRGFDLKHAPLFMD
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEECCCCCCCEEEC
GIPIYVPYDGNVDLGRFFTYDLSELVISKGFASVLYGPNTMGGAINMVSKRPTKEFEGSA
CEEEEEECCCCCCCCCEEEHHHHHHHHHCCCHHEEECCCCCCCCEECCCCCCCHHCCCCC
GTGYSSVDQYHAFANFGTNQGKWYFQGGGSWFDTNGFEMSEHYHNTTYENGGKRNNAYAQ
CCCCCHHHHHHHHHCCCCCCCEEEECCCCCEECCCCCCCHHHHCCCCCCCCCCCCCCCCC
DWKVSGKLGLTPNSTDEYALSYIYQNGEKGVPPYAGDNLTNQKKKFWQWPYWRKQSLYFT
CEEECCEECCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEE
SNTAIGEKSYVKTRAYYDRFKNSLLIFTNDSYSTVTPNSGNRSWYDDYTYGASIEAGTKL
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCCHHHCCEE
ISNNEIKLAFLFKDDVHREDDDATGSPQKRFEERIFTIALEDTIKLTDKLSAILGVSYDF
ECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHCEEEEEEECCHHHHHHHHHHHHCCCCCC
QNTVQAQDYNPIVSGGPNVLHDFDRKNNSDVNAQAALFYAYSDTGKVHAAVARKNRYPSI
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEHHCCCCCCC
KDKYSYRFKTYVPNENLDPEVSNNYEIGVEDTFAKNIRVKSTLFFNDISKYILEVKNVEG
CCCCCEEEEEECCCCCCCCCCCCCEEECCCCHHHCCEEEEEEEEHHHHHHHHHHHCCCCC
TKSQLQNVGHVQRYGYELEANAAITESLEAGMNYTFMYNKNRTSDLVITDLPKHKFFAYG
HHHHHHHCCHHHHCCEEEECCCHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCEEEEC
TYSPIKQLKLFASLEYDTRRYSSSDGVDEAGEFVVINTKASYEAIKDLVLEAGINNLLDR
CCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
DYELTEGYPEAGRTYFVQARYKW
CCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKKQKRRFSVRIKKRGLLAPVLAVSLLSTPAMADDQAPKSDESGDVFTLGNVEVTGKGES
CCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCC
IKNPAVEVVTSEDMLQFDTKTVSEAANLVPGVTLAKMGARNESTVYVRGFDLKHAPLFMD
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEECCCCCCCEEEC
GIPIYVPYDGNVDLGRFFTYDLSELVISKGFASVLYGPNTMGGAINMVSKRPTKEFEGSA
CEEEEEECCCCCCCCCEEEHHHHHHHHHCCCHHEEECCCCCCCCEECCCCCCCHHCCCCC
GTGYSSVDQYHAFANFGTNQGKWYFQGGGSWFDTNGFEMSEHYHNTTYENGGKRNNAYAQ
CCCCCHHHHHHHHHCCCCCCCEEEECCCCCEECCCCCCCHHHHCCCCCCCCCCCCCCCCC
DWKVSGKLGLTPNSTDEYALSYIYQNGEKGVPPYAGDNLTNQKKKFWQWPYWRKQSLYFT
CEEECCEECCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEE
SNTAIGEKSYVKTRAYYDRFKNSLLIFTNDSYSTVTPNSGNRSWYDDYTYGASIEAGTKL
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCCHHHCCEE
ISNNEIKLAFLFKDDVHREDDDATGSPQKRFEERIFTIALEDTIKLTDKLSAILGVSYDF
ECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHCEEEEEEECCHHHHHHHHHHHHCCCCCC
QNTVQAQDYNPIVSGGPNVLHDFDRKNNSDVNAQAALFYAYSDTGKVHAAVARKNRYPSI
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEHHCCCCCCC
KDKYSYRFKTYVPNENLDPEVSNNYEIGVEDTFAKNIRVKSTLFFNDISKYILEVKNVEG
CCCCCEEEEEECCCCCCCCCCCCCEEECCCCHHHCCEEEEEEEEHHHHHHHHHHHCCCCC
TKSQLQNVGHVQRYGYELEANAAITESLEAGMNYTFMYNKNRTSDLVITDLPKHKFFAYG
HHHHHHHCCHHHHCCEEEECCCHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCEEEEC
TYSPIKQLKLFASLEYDTRRYSSSDGVDEAGEFVVINTKASYEAIKDLVLEAGINNLLDR
CCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
DYELTEGYPEAGRTYFVQARYKW
CCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11598060 [H]