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The map label for this gene is grpE

Identifier: 118579829

GI number: 118579829

Start: 1498101

End: 1498673

Strand: Reverse

Name: grpE

Synonym: Ppro_1405

Alternate gene names: 118579829

Gene position: 1498673-1498101 (Counterclockwise)

Preceding gene: 118579830

Following gene: 118579828

Centisome position: 37.39

GC content: 59.86

Gene sequence:

>573_bases
ATGAAAAAGCATGTAACAGAAGAACAGAAAACTTCCGCCGCACCCGAGGCGGAACAGGCATCTCCCGAAAGCAGCGCTGC
CGAGGCTGCCACCCCGGAAGAACGCATCTCGCGGCTCGAGGAGCAACTGGCCGCCAAAGAGGCCGAGTGTCGCGAGAACT
GGGACCGTTTCGTGCGTGAACGCGCCGATCTGGAGAACTTCCGCAAACGGTCCAACCGGGAAAAGGAAGAGCTGCTCAAC
TACGGTACAAAATCACTACTTGAAGAGATACTGCCGGTGGTGGACAACCTGGAGCGAGCGCTTTCCCATGCCAACGAAAA
CGGTTCGACAGGCCTGACGGAAGGGGTCCAGATGATCCACGGCTTGCTGCTCAACGCCATGAAGAAGTTCGGAGTCACCC
CCCTGGAAACGTCCGGCGCCCCGTTCGACCCCTCCTTCCACCAGGCAATGACCCAGATCCCCACCGATGAGCACCCACCC
AATACGGTGGTGGAGGAGTTCCAGAAGGGCTACCTGCTCAAGGAGCGCCTGCTGCGTCCCGCCATGGTTTCGGTGGCCAC
GGCGCCCAAGTAA

Upstream 100 bases:

>100_bases
TCGACTATACAGCCCGCCTGTTGAGCCGTTTCCTGAACAACGACTGACCGCCAGGACATTCGCATCAAAAATGAAACGCA
CAAAAAGGACTCACTGAACC

Downstream 100 bases:

>100_bases
GCCCCGTTCCTCTCCGCGGAGGGTAAGCGGCGAACTTATTTTTCCCGGCGCCCTTGTTTTTCACCCCAACGATGACTACT
TTTGAGAGCAGAAAGAATTG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKKHVTEEQKTSAAPEAEQASPESSAAEAATPEERISRLEEQLAAKEAECRENWDRFVRERADLENFRKRSNREKEELLN
YGTKSLLEEILPVVDNLERALSHANENGSTGLTEGVQMIHGLLLNAMKKFGVTPLETSGAPFDPSFHQAMTQIPTDEHPP
NTVVEEFQKGYLLKERLLRPAMVSVATAPK

Sequences:

>Translated_190_residues
MKKHVTEEQKTSAAPEAEQASPESSAAEAATPEERISRLEEQLAAKEAECRENWDRFVRERADLENFRKRSNREKEELLN
YGTKSLLEEILPVVDNLERALSHANENGSTGLTEGVQMIHGLLLNAMKKFGVTPLETSGAPFDPSFHQAMTQIPTDEHPP
NTVVEEFQKGYLLKERLLRPAMVSVATAPK
>Mature_190_residues
MKKHVTEEQKTSAAPEAEQASPESSAAEAATPEERISRLEEQLAAKEAECRENWDRFVRERADLENFRKRSNREKEELLN
YGTKSLLEEILPVVDNLERALSHANENGSTGLTEGVQMIHGLLLNAMKKFGVTPLETSGAPFDPSFHQAMTQIPTDEHPP
NTVVEEFQKGYLLKERLLRPAMVSVATAPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=168, Percent_Identity=33.9285714285714, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1788967, Length=175, Percent_Identity=36, Blast_Score=117, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17552458, Length=150, Percent_Identity=30.6666666666667, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=157, Percent_Identity=31.2101910828025, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653432, Length=195, Percent_Identity=31.7948717948718, Blast_Score=93, Evalue=8e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PELPD (A1ANV1)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901079.1
- ProteinModelPortal:   A1ANV1
- SMR:   A1ANV1
- STRING:   A1ANV1
- GeneID:   4571974
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1405
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   A1ANV1
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21281; Mature: 21281

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHVTEEQKTSAAPEAEQASPESSAAEAATPEERISRLEEQLAAKEAECRENWDRFVRE
CCCCCCHHHHHHCCCCHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RADLENFRKRSNREKEELLNYGTKSLLEEILPVVDNLERALSHANENGSTGLTEGVQMIH
HHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
GLLLNAMKKFGVTPLETSGAPFDPSFHQAMTQIPTDEHPPNTVVEEFQKGYLLKERLLRP
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AMVSVATAPK
HHHHHHCCCC
>Mature Secondary Structure
MKKHVTEEQKTSAAPEAEQASPESSAAEAATPEERISRLEEQLAAKEAECRENWDRFVRE
CCCCCCHHHHHHCCCCHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RADLENFRKRSNREKEELLNYGTKSLLEEILPVVDNLERALSHANENGSTGLTEGVQMIH
HHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
GLLLNAMKKFGVTPLETSGAPFDPSFHQAMTQIPTDEHPPNTVVEEFQKGYLLKERLLRP
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AMVSVATAPK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA