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The map label for this gene is ogt [H]

Identifier: 118579766

GI number: 118579766

Start: 1416964

End: 1417437

Strand: Direct

Name: ogt [H]

Synonym: Ppro_1338

Alternate gene names: 118579766

Gene position: 1416964-1417437 (Clockwise)

Preceding gene: 118579764

Following gene: 118579768

Centisome position: 35.35

GC content: 63.92

Gene sequence:

>474_bases
ATGGTGCATTTTCAGGAAACCTGTCTGGGACGAATCGGCATCGCCGAGGAGGGGGGAGGCATCAGCCACCTCTTCCTGGG
GGGGGATAGCGTGCCGCGGGAGATGGAACAGGGCCAGACGCCGCTCTTGCGAGAAGCTTTTCAGCAACTTGAACGCTATC
TGGCGGGAGAACTGACCGATTTTTCCCTGGCGCTCATGCCGGACGGCACCCCCTTCATGCAGCGGGTGTGGCAGGAACTC
CGCCGGGTGCCCTACGGCCGGACGGCCAGCTACCGGGATATCGCCACCGCACTGGGCAACCCCAAAGCCGTGCGTGCGGT
GGGGATGGCCAACAACAGGAATCCGATCCCCATCTTCATCCCCTGCCACCGGATCATCGGCTGTAGCGGGAAGCTGGTGG
GCTACCGCGGCGGCTTGGAGATGAAGAGGCGGCTGCTGGAACTGGAGAGGGGGAGAGCGTGCCCTCAGGGATAG

Upstream 100 bases:

>100_bases
GTTGGGCACACCGGACCTCCTTCGGGAGGGGCAGTGTAAACCGCGCAGACTGCCCTTGTCAAAGGAGCGTTATGCTGCGG
GCATAGATAAGGAGGAAGCC

Downstream 100 bases:

>100_bases
AGGGTGATCAGCGCCACGCCGCAGAGCATCAGCAGTGTGCCTGCCAGTCGCCCGGCAATTCCCTGCTCATGGAACAGGAC
GTGTCCGTAGATGACTCCCA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MVHFQETCLGRIGIAEEGGGISHLFLGGDSVPREMEQGQTPLLREAFQQLERYLAGELTDFSLALMPDGTPFMQRVWQEL
RRVPYGRTASYRDIATALGNPKAVRAVGMANNRNPIPIFIPCHRIIGCSGKLVGYRGGLEMKRRLLELERGRACPQG

Sequences:

>Translated_157_residues
MVHFQETCLGRIGIAEEGGGISHLFLGGDSVPREMEQGQTPLLREAFQQLERYLAGELTDFSLALMPDGTPFMQRVWQEL
RRVPYGRTASYRDIATALGNPKAVRAVGMANNRNPIPIFIPCHRIIGCSGKLVGYRGGLEMKRRLLELERGRACPQG
>Mature_157_residues
MVHFQETCLGRIGIAEEGGGISHLFLGGDSVPREMEQGQTPLLREAFQQLERYLAGELTDFSLALMPDGTPFMQRVWQEL
RRVPYGRTASYRDIATALGNPKAVRAVGMANNRNPIPIFIPCHRIIGCSGKLVGYRGGLEMKRRLLELERGRACPQG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=79, Percent_Identity=48.1012658227848, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1788542, Length=151, Percent_Identity=42.3841059602649, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=45.6310679611651, Blast_Score=93, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=86, Percent_Identity=47.6744186046512, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=86, Percent_Identity=47.6744186046512, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=42.6829268292683, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17455; Mature: 17455

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHFQETCLGRIGIAEEGGGISHLFLGGDSVPREMEQGQTPLLREAFQQLERYLAGELTD
CCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCC
FSLALMPDGTPFMQRVWQELRRVPYGRTASYRDIATALGNPKAVRAVGMANNRNPIPIFI
CEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHEEEECCCCCCCCCEEEE
PCHRIIGCSGKLVGYRGGLEMKRRLLELERGRACPQG
ECHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVHFQETCLGRIGIAEEGGGISHLFLGGDSVPREMEQGQTPLLREAFQQLERYLAGELTD
CCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCC
FSLALMPDGTPFMQRVWQELRRVPYGRTASYRDIATALGNPKAVRAVGMANNRNPIPIFI
CEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHEEEECCCCCCCCCEEEE
PCHRIIGCSGKLVGYRGGLEMKRRLLELERGRACPQG
ECHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]