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The map label for this gene is ilvK [H]

Identifier: 118579763

GI number: 118579763

Start: 1410655

End: 1411728

Strand: Reverse

Name: ilvK [H]

Synonym: Ppro_1335

Alternate gene names: 118579763

Gene position: 1411728-1410655 (Counterclockwise)

Preceding gene: 118579765

Following gene: 118579762

Centisome position: 35.22

GC content: 60.89

Gene sequence:

>1074_bases
ATGGATATTCAAGTCCTTCCGCTCCCCGCGGAAAAAATGAAACAGAAAGCACAAGACGAAACCAAGCTCAGCTTTGGCCG
GCAATTCACCGACCGCATGCTGCTGGTGGAATGGAAGGCCGACCAGGGGTGGTGCGACGCGCGCATCCAGCCCTACGCTC
CCTTTGTACTTGATCCCTCCTGCCTGGTGTTCCACTACGCCCAGGAGATATTCGAAGGCCTGAAGGCCTACAAATGGGCC
GACGGCAGGATAGCGCTGTTCCGTCCCGAGATGAACGCACGGCGCTTCAACCAGTCCGCCGACCGGCTCTGCATGCCGGA
CGTGCCCGAGGAACTGTTCCTGGGTGGCATCGAGAAACTGCTCTCCCTGGAGAAGGAGTGGATTCCCGGCGCGCCGGGAA
CCTCGCTCTACATCCGGCCGGCCATGATCGCCGTGGAACCGGTGCTGGGCGTCAAGCCGTCAAGCCACTACTACTTCTTC
GTCATCCTCTCCCCGGTGGGCGCCTACTACTCCGCAGGCTTCAACCCGGTCAGCATCATGGTGGAAGACCACTACGTGCG
CGCCGTACCCGGCGGCACCGGCGAAGCCAAAACCGGCGGCAACTACGCCAGCTCGCTCAAGGGGGCGCTGGAGGCCAAGA
ACAAGGGCTATGACCAGGTGCTCTGGCTGGACGGCCGCGAACAGCGCTATGTCGAGGAAGTCGGATCCATGAACATGTTC
TTCGTCTATGGCGACACAATCGTTACCGCGGCCATGAACGGCTCGATCCTCTCCGGCATCACCCGCGATTCCATACTCAA
ACTGGCTGTCTCATTGGGATACCGGGCAGAGGAACGCAGCATCGAAATCAATGAGTTGATGGACGACATCAGGGGCGGCA
AGGTGACCGAGGCCTTTGGCAGCGGCACCGCGGCCGTGGTCTCGCCGGTGGGTAAACTCTGCTTCAAGGACGAGGTCGTC
CACCTGAGCGGAGGCACGGTGGGCGCGATAACCCAGAAACTGTACGACACCCTGACCGGCATCCAGACCGGAAAAATCAA
GGACGAGTTCGGCTGGGTCAGGTTCGTCGCCTGA

Upstream 100 bases:

>100_bases
CATGTTTTCCACGAATTTGTCAACAGGGTTTTTGGCCTTGCCGTAACGTAGCGGATATGATACAAAAATCTTCATTTAAT
GTTGTCCCGGAGGCGTTGTC

Downstream 100 bases:

>100_bases
CAGAGCGCGATCACGCACAATCCGCGCAGCCGCTCCCGGGCACGAGCCGTTTCCGGGGACGGCTGCGCAGGCAAATCCAC
AACCATGAGTCCAGCACACA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MDIQVLPLPAEKMKQKAQDETKLSFGRQFTDRMLLVEWKADQGWCDARIQPYAPFVLDPSCLVFHYAQEIFEGLKAYKWA
DGRIALFRPEMNARRFNQSADRLCMPDVPEELFLGGIEKLLSLEKEWIPGAPGTSLYIRPAMIAVEPVLGVKPSSHYYFF
VILSPVGAYYSAGFNPVSIMVEDHYVRAVPGGTGEAKTGGNYASSLKGALEAKNKGYDQVLWLDGREQRYVEEVGSMNMF
FVYGDTIVTAAMNGSILSGITRDSILKLAVSLGYRAEERSIEINELMDDIRGGKVTEAFGSGTAAVVSPVGKLCFKDEVV
HLSGGTVGAITQKLYDTLTGIQTGKIKDEFGWVRFVA

Sequences:

>Translated_357_residues
MDIQVLPLPAEKMKQKAQDETKLSFGRQFTDRMLLVEWKADQGWCDARIQPYAPFVLDPSCLVFHYAQEIFEGLKAYKWA
DGRIALFRPEMNARRFNQSADRLCMPDVPEELFLGGIEKLLSLEKEWIPGAPGTSLYIRPAMIAVEPVLGVKPSSHYYFF
VILSPVGAYYSAGFNPVSIMVEDHYVRAVPGGTGEAKTGGNYASSLKGALEAKNKGYDQVLWLDGREQRYVEEVGSMNMF
FVYGDTIVTAAMNGSILSGITRDSILKLAVSLGYRAEERSIEINELMDDIRGGKVTEAFGSGTAAVVSPVGKLCFKDEVV
HLSGGTVGAITQKLYDTLTGIQTGKIKDEFGWVRFVA
>Mature_357_residues
MDIQVLPLPAEKMKQKAQDETKLSFGRQFTDRMLLVEWKADQGWCDARIQPYAPFVLDPSCLVFHYAQEIFEGLKAYKWA
DGRIALFRPEMNARRFNQSADRLCMPDVPEELFLGGIEKLLSLEKEWIPGAPGTSLYIRPAMIAVEPVLGVKPSSHYYFF
VILSPVGAYYSAGFNPVSIMVEDHYVRAVPGGTGEAKTGGNYASSLKGALEAKNKGYDQVLWLDGREQRYVEEVGSMNMF
FVYGDTIVTAAMNGSILSGITRDSILKLAVSLGYRAEERSIEINELMDDIRGGKVTEAFGSGTAAVVSPVGKLCFKDEVV
HLSGGTVGAITQKLYDTLTGIQTGKIKDEFGWVRFVA

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=353, Percent_Identity=42.4929178470255, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI296010906, Length=353, Percent_Identity=42.2096317280453, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI38176287, Length=353, Percent_Identity=42.2096317280453, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI50658084, Length=320, Percent_Identity=41.5625, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI296010902, Length=350, Percent_Identity=37.4285714285714, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI296010900, Length=353, Percent_Identity=37.6770538243626, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI258614015, Length=261, Percent_Identity=41.7624521072797, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI48994963, Length=320, Percent_Identity=30, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568601, Length=367, Percent_Identity=39.7820163487738, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17565728, Length=361, Percent_Identity=34.0720221606648, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322608, Length=375, Percent_Identity=41.0666666666667, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322002, Length=371, Percent_Identity=41.5094339622642, Blast_Score=274, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24641779, Length=352, Percent_Identity=38.6363636363636, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39473; Mature: 39473

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQVLPLPAEKMKQKAQDETKLSFGRQFTDRMLLVEWKADQGWCDARIQPYAPFVLDPS
CCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEECCH
CLVFHYAQEIFEGLKAYKWADGRIALFRPEMNARRFNQSADRLCMPDVPEELFLGGIEKL
HHHHHHHHHHHHHHHHEEECCCEEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHH
LSLEKEWIPGAPGTSLYIRPAMIAVEPVLGVKPSSHYYFFVILSPVGAYYSAGFNPVSIM
HHHHHHHCCCCCCCEEEEEEEEEEEEHHHCCCCCCCEEEEEEECCCCHHHHCCCCEEEEE
VEDHYVRAVPGGTGEAKTGGNYASSLKGALEAKNKGYDQVLWLDGREQRYVEEVGSMNMF
EECCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCEEE
FVYGDTIVTAAMNGSILSGITRDSILKLAVSLGYRAEERSIEINELMDDIRGGKVTEAFG
EEECCEEEEEECCCHHHHCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCCEEEHHHC
SGTAAVVSPVGKLCFKDEVVHLSGGTVGAITQKLYDTLTGIQTGKIKDEFGWVRFVA
CCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MDIQVLPLPAEKMKQKAQDETKLSFGRQFTDRMLLVEWKADQGWCDARIQPYAPFVLDPS
CCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEECCH
CLVFHYAQEIFEGLKAYKWADGRIALFRPEMNARRFNQSADRLCMPDVPEELFLGGIEKL
HHHHHHHHHHHHHHHHEEECCCEEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHH
LSLEKEWIPGAPGTSLYIRPAMIAVEPVLGVKPSSHYYFFVILSPVGAYYSAGFNPVSIM
HHHHHHHCCCCCCCEEEEEEEEEEEEHHHCCCCCCCEEEEEEECCCCHHHHCCCCEEEEE
VEDHYVRAVPGGTGEAKTGGNYASSLKGALEAKNKGYDQVLWLDGREQRYVEEVGSMNMF
EECCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCEEE
FVYGDTIVTAAMNGSILSGITRDSILKLAVSLGYRAEERSIEINELMDDIRGGKVTEAFG
EEECCEEEEEECCCHHHHCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCCEEEHHHC
SGTAAVVSPVGKLCFKDEVVHLSGGTVGAITQKLYDTLTGIQTGKIKDEFGWVRFVA
CCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]